Mycobacterium phage Liloa
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Detailed Information for Phage Liloa
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKasie Stephens
Year Found2015
Location FoundCullowhee, NC USA
Finding InstitutionWestern Carolina University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates35.485086 N, 82.996489 W Map
Discovery NotesMy sample was taken from a compost sample in a WCU faculty member's backyard. Dr. Marth's sample had loads of phage in it. My average tail size of my phage was 0.204 um and the average head size was 0.062325 um. My DNA yield was 179.4 ng/um.
Naming NotesLiloa stands for "Lil Orphan Annie". I acquired Liloa when my Phage Hunters instructor wanted me to further move his sample along. Since I was having a difficult time isolating my own phage I took Liloa under my wing and grew her up from the very beginning. She was an orphan and I so to speak "adopted" her.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotation StatusNot sequenced
Plaque NotesMy phage produces small and medium sized lytic plaques that are characteristic of my phage. Plaques can generally be seen after 24 hours and my plaque sizes stayed consistent the entire duration of my Phage Hunters course.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2*10^11 pfu/ml
Date of SEA Lysate Titering Oct 21, 2015
Pitt Freezer Box# 19
Pitt Freezer Box Grid# G7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload