Mycobacterium phage Lionlamb89
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| Detailed Information for Phage Lionlamb89 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kimiko Gray and Vanessa Yepez |
| Year Found | 2017 |
| Location Found | Fayetteville, NC USA |
| Finding Institution | Fayetteville State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 35.033 N, 79.02 W Map |
| Discovery Notes | - Discovered August 27, 2017 in a field beside Food Lion. - Very warm that day, in the upper eighties. - Soil sample was taken next to an anthill, a couple of inches down into the topsoil. - Environment was fully exposed to sunlight. - Surrounding vegetation was scrub and dry grass; grew more lush further infield. |
| Naming Notes | The phage was found in a field beside a local Food Lion ("Lion"), but during the initial amplification step, it fizzled out out of the culture dishes, revealing its relatively delicate nature ("lamb"). 89 was added at the end was just because. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Phage is lysogenic due to a turbid plaque morphology. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques are turbid (meaning the phage is lysogenic), and are 1-2 milimeters in diameter. Lionlamb89 requires a higher concentration per aliquot to manifest on the culture plates. NOTE: Halfway through the semester, we used a new culture of M. smeg, and the plaques in the culture plates appeared only 1 mm in length. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.2e8 PFUs/ml |
| Pitt Freezer Box# | 65 |
| Pitt Freezer Box Grid# | H11 |
| Available Files | |
| Plaque Picture | Download |