Mycobacterium phage Luboph
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Detailed Information for Phage Luboph
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByParker Sevier
Year Found2017
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates34.0665 N, 118.452 W Map
Discovery NotesPhage Luboph was isolated by Parker Sevier on 4/11/17 from moist surface soil collected from urban area, damp with plant growth. Air temp at time of collection was 20C. Luboph was isolated by enrichment plating at 30C (10X 7H9/glycerol medium, CaCl2, AD Supplement, M. smeg).

Characterization was performed by Team Natural Cellection (Nikolina Walas, Aika Miikeda, Lauren Brenner, Sai Alam) and Team Sick Virions (Alice Sukhina, Jinhua Shen, Andy Wei, Crystal Ma).

Phage Luboph appears to be a temperate phage that demonstrated turbid or clear plaque morphologies under different plating conditions. Turbid plaques occur consistently at 35˚C and 37˚C, and clear plaques are seen at 30˚C . Putative lysogen were isolated that release phage.

Name of Individual who collected sample: Parker Sevier
Midi-Lysate (Archive) Titer: 1x10e11 PFU/mL
Date of Midi-Lysate Titer: 5/10/17

Instructor: Dr. Jordan Moberg Parker
Teaching Assistant: Michelle Bina
Undergraduate Assistant: Rachel Zhu
Naming NotesLuboph is an English alphabet phonetical representation of a Russian word that means Love.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Plaque NotesPhage Luboph appears to be a temperate phage that demonstrated turbid or clear plaque morphologies under different plating conditions. Turbid plaques occur consistently at 35˚C and 37˚C, and clear plaques are seen at 30˚C .

Average size (and size range) diameter in mm: 3mm (1.0 - 6.0 mm in various environments but most consistently 3 mm)
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload