| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Lunahalysis |
| Found By | Megan Dillingham |
| Year Found | 2016 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.980128 N, 87.511206 W Map |
| Discovery Notes | Sample was taken from a forested area located near a water park. Soil was near water, suggesting that during high waters (such as after a heavy rain), the sample would be submerged.
Soil was very moist, but not saturated. It was very smooth and contained a few small roots.
Date of collection: 8/28/2016
Time of collection: 4:20 P.M.
Depth of collection: 0cm-2.5cm
Direction facing during collection: 42° NE
Temperature (at time of collection):32°C
Weather summery: Precipitation at 15%, humidity at 57%, partly cloudy. |
| Naming Notes | Lunahalos has been observed to form three unique plaque morphologies: completely clear, completely turbid, and clear with a turbid ring. "Luna," the Latin word for moon, represents the different phases. "Halos," the Latin term for halo, represents the turbid ring. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 12, 2022 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 251 |
| Genome length (bp) | 59708 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Lunahalos is a lysogenic phage.In addition, it has produced clear-plaque mutants. |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | Lunahalos plaque morphology varies. Upon initial observation, plaques were small (2mm) and turbid. Later plates contained plaques with clear centers with turbid rings, turbid plaques between .5mm and 2mm, and clear-plaque mutants. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OQ709219 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2016WEKUlunahalosMD |
| SEA Lysate Titer |
2.9 × 10^9 pfu/mL |
| Date of SEA Lysate Titering |
Oct 20, 2016 |
| Pitt Freezer Box# |
37 |
| Pitt Freezer Box Grid# |
C9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
