| Detailed Information for Phage Lysocool |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Lucas McClish & Tony Wolfe |
| Year Found | 2014 |
| Location Found | Columbus, OH United States |
| Finding Institution | The Ohio State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | Unavailable |
| Discovery Notes | Enrichment plates were obtained from other classmates cultures due to lack of phage from soil sample. Agarose gel electrophoresis produced what appeared to be unique DNA. |
| Naming Notes | It's a play of words off of lysosomes. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 719 |
| Genome length (bp) | 154462 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Other Cluster Members |
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| Plaque Notes | Was mostly lytic morphology with plaques about the size of an eraser head. For final dilution, 10^-5 appeared half lytic, half temperate; 10^-4 however appeared purely lytic. |
| Morphotype | Myoviridae |
| Final annotation complete? | No |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
