| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Lungile Marcia Mbambo |
| Year Found | 2013 |
| Location Found | Durban (KwaMashu), South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.835029 S , 31.014277 E Map |
| Discovery Notes | the soil sample was taken from KwaMashu township garden, the phage in lower dilutions(10-1) destroys all bacterial cells, it was observed the on the second day of the course which was excitting.the phage was purified 2 times for EM 3rd times for Titer. |
| Naming Notes | the name of the phage comes from the finder`s name and the behaviour of the phage to the solid agar surface. Malithi means "queen of war", the phage ate ever cell in the growth medium plate indicating that it is dominating the environment. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 13, 2014 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent Sequencing |
| Approximate Shotgun Coverage | 375 |
| Sanger Finishing Reactions | 11 |
| Genome length (bp) | 46870 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCTGCCGCCCGA |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | the plaques had a smooth surface, not toatally round in shape but some were and grew in different sizes. some of the plaques were bigger than the others while others remained tiny. increasing incubation time results in more clearer plaques |
| Morphotype | Siphoviridae |
| Number of Genes | 80 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KP027200 |
| Refseq Number | NC_026605 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
E8 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
