Microbacterium phage MementoMori
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Detailed Information for Phage MementoMori
Discovery Information
Isolation HostMicrobacterium paraoxydans NWU1
Found ByElijah Washburn
Year Found2016
Location FoundLaVista, NE United States of America
Finding InstitutionNebraska Wesleyan University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature67°C
GPS Coordinates41.187567 N, 95.972347 W Map
Discovery NotesThis phage was isolated near the base of an oak tree. I dug about five inches down attempting to find soil near a root.
Naming NotesThis phage had very small plaques from the very first isolation. The plaques size and number made it difficult to tell if I had truly isolated it, as well it was very difficult to find the correct titre to get a webbed plate, I persevered through the challenges and eventually completed my work on the phage. I decided to name it memento mori in honor of the great tradition the ancient Romans held after victory, memento mori is meant to remind me to be humble while i think of my work here, and to thank all those who helped me through it.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 25, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1128
Genome length (bp)55572
Character of genome endsCircularly Permuted
GC Content70.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEI
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members

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Annotation StatusIn GenBank
Plaque NotesThis phage had very small clear plaques, that were circular and often seemed to overlap each other at lower titres.
Has been Phamerated?Yes
Gene List

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MementoMori_1
(ParB-like nuclease domain)

MementoMori_2
(queuine tRNA-ribosyltransferase)

MementoMori_3
(terminase, small subunit)

MementoMori_4
(terminase, large subunit)

MementoMori_5

MementoMori_6

MementoMori_7
(ThyX thymidylate synthase)

MementoMori_8

MementoMori_9

MementoMori_10

MementoMori_11

MementoMori_13
(portal protein)

MementoMori_14

MementoMori_15
(capsid morphogenesis protein, MuF-like)

MementoMori_16

MementoMori_17

MementoMori_18

MementoMori_19

MementoMori_20

MementoMori_21
(capsid maturation protease)

MementoMori_22

MementoMori_23
(major capsid protein)

MementoMori_24

MementoMori_25

MementoMori_26
(major tail protein)

MementoMori_27

MementoMori_28

MementoMori_29

MementoMori_30

MementoMori_31

MementoMori_32
(tape measure protein)

MementoMori_33
(minor tail protein)

MementoMori_34
(minor tail protein)

MementoMori_35
(minor tail protein)

MementoMori_36
(minor tail protein)

MementoMori_37

MementoMori_38

MementoMori_39
(lysin A)

MementoMori_40

MementoMori_41
(membrane protein)

MementoMori_42

MementoMori_43
(membrane protein)

MementoMori_44

MementoMori_45
(RecE-like exonuclease)

MementoMori_46
(ssDNA binding protein, ERF family)

MementoMori_47

MementoMori_48

MementoMori_49

MementoMori_50

MementoMori_51

MementoMori_52

MementoMori_53
(HNH endonuclease)

MementoMori_54
(helix-turn-helix DNA binding domain)

MementoMori_55

MementoMori_56

MementoMori_57

MementoMori_58

MementoMori_59
(helix-turn-helix DNA binding domain)

MementoMori_60

MementoMori_61
(ssDNA binding protein)

MementoMori_62

MementoMori_63

MementoMori_64

MementoMori_65
(excise)

MementoMori_66
(helix-turn-helix DNA binding protein)

MementoMori_67

MementoMori_68

MementoMori_69

MementoMori_70

MementoMori_71

MementoMori_72

MementoMori_73

MementoMori_74

MementoMori_75

MementoMori_76

MementoMori_77

MementoMori_78

MementoMori_79

MementoMori_80

MementoMori_81

MementoMori_82

MementoMori_83

MementoMori_84

MementoMori_85

MementoMori_86

MementoMori_87

MementoMori_88

MementoMori_89

MementoMori_90

MementoMori_91

MementoMori_92

MementoMori_93

MementoMori_94

MementoMori_95

MementoMori_96

MementoMori_97
(ParB-like nuclease domain)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH271303
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 45
Pitt Freezer Box Grid# E12
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload