Mycobacterium phage MonsterSteve
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Detailed Information for Phage MonsterSteve
Discovery Information
Isolation HostMycobacterium smegmatis ATCC 607
Found ByMarta Heinz, Paige Beckman
Year Found2018
Location FoundBoulder, CO United States
Finding InstitutionUniversity of Colorado at Boulder
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates40.009 N, 105.2687 W Map
Discovery NotesSample site is a grassy mulched garden next to the Gold Biosciences building on the CU Campus. Soil sample collected at 10:16 am on 2/6/2018. Approximate temperature at time of collection was -1˚C.
Naming NotesSteve was an arbitrary name selection which was modified as "MonsterSteve" when the phage cleared all of our plates during the titer assay of a high titer lysate.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterA
SubclusterA1
Cluster Life CycleTemperate
Other Cluster Members

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Afua (None)

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WalterMcMickey (A10)

Bachome (A11)

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Lucivia (A11)

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Munch (A11)

Salz (A11)

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DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Steamy (A12)

Phlei (A13)

Jeeves (A14)

Luchador (A14)

Onism (A14)

Anon (A15)

JSwag (A15)

KatherineG (A15)

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Annotation StatusNot sequenced
Plaque NotesDuring a plaque assay, MonsterSteve produced 125 2-5mm in diameter cloudy plaques. We incubated the plate for 48 hrs at 37˚C.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload