| Detailed Information for Phage MrAK | |
| Discovery Information | |
| Isolation Host | Propionibacterium acnes ATCC 6919 |
| Found By | Greg Asatrian |
| Year Found | 2011 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.065 N, 118.4425 W Map |
| Discovery Notes | Phage isolation performed by Gregory Asatrian, Kevork Khadarian,Cherise Meyerson, and Laya Reddy (UCLA). DNA preparation performed by Krisanavane Reddi (UCLA). Assembly performed by William Villella (UCLA) and confirmed by Charles Bowman (University of Pittsburgh). Genome annotation performed by Manami Kawakita, Jaclyn Matsuura, Charmian Wong, Jamie Yabuno, Kylie Wilson, Katie Thure, and Samantha Capati (UCLA). Quality control of annotation results performed by Jordan Moberg Parker and Dallas Mould (UCLA). |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 26, 2015 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 254 |
| Genome length (bp) | 29726 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TCGTACGGCTT |
| GC Content | 54.4% |
| Sequencing Notes | There is a polymorphism - equal peaks for G and T at 15141. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BU |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAquarius ATCC29399BC ATCC29399BT Attacne BruceLethal Cota DrParker Enochoraptor Enoki Keiki Kubed Lauchelly Leviosa LilBandit MEAK Moyashi MrAK Ouroboros P1.1 P100.1 P100A P100D P101A P104A P104B P105 P106A P106C P106I P106L P106M P107A P107C P108C P14.4 P9.1 PA6 PAD20 PAS50 Pirate Procrass1 QueenBey Rileysaurus SKKY Solid Stormborn Supernova Wizzo |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | clear and circular with smooth edges; average diameter of 7.3 mm |
| Morphotype | Siphoviridae |
| Number of Genes | 46 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewMrAK_1 MrAK_2 MrAK_3 MrAK_4 MrAK_5 MrAK_6 MrAK_7 MrAK_8 MrAK_9 MrAK_10 MrAK_11 MrAK_12 MrAK_13 MrAK_14 MrAK_15 MrAK_16 MrAK_17 MrAK_18 MrAK_19 MrAK_20 MrAK_21 MrAK_22 MrAK_23 MrAK_24 MrAK_25 MrAK_26 MrAK_27 MrAK_28 MrAK_29 MrAK_30 MrAK_31 MrAK_32 MrAK_33 MrAK_34 MrAK_35 MrAK_36 MrAK_37 MrAK_38 MrAK_39 MrAK_40 MrAK_41 MrAK_42 MrAK_43 MrAK_44 MrAK_45 MrAK_46 |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KR337643 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | Mr. AK (PAPB2) |
| SEA Lysate Titer | 7.5x10^8 |
| Date of SEA Lysate Titering | Nov 23, 2011 |
| Pitt Freezer Box# | 28 |
| Pitt Freezer Box Grid# | D5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
