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Detailed Information for Phage MrAK
Discovery Information
Isolation HostPropionibacterium acnes ATCC 6919
Found ByGreg Asatrian
Year Found2011
Location FoundLos Angeles, CA United States
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature37°C
GPS Coordinates34.065 N, 118.4425 W Map
Discovery NotesPhage isolation performed by Gregory Asatrian, Kevork Khadarian,Cherise Meyerson, and Laya Reddy (UCLA). DNA preparation performed by Krisanavane Reddi (UCLA). Assembly performed by William Villella (UCLA) and confirmed by Charles Bowman (University of Pittsburgh). Genome annotation performed by Manami Kawakita, Jaclyn Matsuura, Charmian Wong, Jamie Yabuno, Kylie Wilson, Katie Thure, and Samantha Capati (UCLA). Quality control of annotation results performed by Jordan Moberg Parker and Dallas Mould (UCLA).
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 26, 2015
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage254
Genome length (bp)29726
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTCGTACGGCTT
GC Content54.4%
Sequencing NotesThere is a polymorphism - equal peaks for G and T at 15141.
Fasta file available?Yes: Download fasta file
Characterization
ClusterBU
Subcluster--
Other Cluster Members
Plaque Notesclear and circular with smooth edges;
average diameter of 7.3 mm
MorphotypeSiphoviridae
Final annotation complete?No
Number of Genes46
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKR337643
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload