Mycobacterium phage Muddy
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Detailed Information for Phage Muddy
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLilli Holst
Year Found2010
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramUnavailable
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates29.8587 S, 31.0218 E Map
Discovery NotesMuddy was the 1400th phage entered into the Mycobacteriophage DataBase!

This sample was scraped from the underside of a partially decomposed aubergine and was therefore largely decomposed vegetable matter. The sample was dark, moist, and worms and other insects were present.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 27, 2012
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage400
Sanger Finishing Reactions8
Genome length (bp)48228
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGTAGCGGCTT
GC Content58.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterSingleton
Subcluster--
Other Cluster Members
Plaque NotesPinprick sized clear circular plaques, but some lessening of turbidity in one direction in some cases.
MorphotypeSiphoviridae
Final annotation complete?Yes
Number of Genes71
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKF024728
Refseq NumberNC_022054
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload