The Actinobacteriophage Database

Mycobacterium phage Nicholasp3

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Detailed Information for Phage Nicholasp3
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Zach C & Raul O
Year Found	2012
Location Found	El Paso, TX USA
Finding Institution	University of Texas at El Paso
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	No
Isolation Temperature	Not entered
GPS Coordinates	31.8 N, 106.44 W Map
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Feb 5, 2013
Sequencing Facility	Virginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method	454 Pyrosequencing
Approximate Shotgun Coverage	1060
Sanger Finishing Reactions	15
Genome length (bp)	75822
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	TCGATCAGCC
GC Content	58.9%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	L
Subcluster	L2
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Acquire49 (L1) Appletree2 (L1) AvadaKedavra (L1) Calm (L1) CicholasNage (L1) DirkDirk (L1) Enceladus (L1) Halena (L1) JoeDirt (L1) LeBron (L1) MAckerman (L1) OhShagHennessy (L1) Rose5 (L1) Silverleaf (L1) Tyson (L1) UPIE (L1) Wamburgrxpress (L1) Wyatt2 (L1) Zaria (L1) Archie (L2) Baoshan (L2) Bazzle (L2) BigCheese (L2) BobsGarage (L2) Breezona (L2) Claus (L2) Crossroads (L2) DrSeegs (L2) Faith1 (L2) Finemlucis (L2) Gabriela (L2) Gardann (L2) GuuelaD (L2) Hafay (L2) Itos (L2) Kahlid (L2) Lewan (L2) LilDestine (L2) Loadrie (L2) Miley16 (L2) MkaliMitinis3 (L2) Netyap (L2) Nicholasp3 (L2) Rumpelstiltskin (L2) Tourach (L2) Vetrix (L2) Wigglewiggle (L2) Wilder (L2) Winky (L2) Zakai (L2) ZhongYanYuan (L2) Bellis (L3) Clautastrophe (L3) DuncansLeg (L3) Ellson (L3) Finnry (L3) Jobypre (L3) Jubie (L3) Kingsolomon (L3) Krypton555 (L3) Lolly9 (L3) Lumos (L3) MiniLon (L3) MiniMac (L3) Moostard (L3) MsGreen (L3) Nicholas (L3) Samty (L3) Snenia (L3) Whirlwind (L3) Bromden (L4) Chaser (L4) Douge (L4) DyoEdafos (L4) Aegeus (L5) Baudelaire (L5)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Nicholasp3_1 Nicholasp3_2 Nicholasp3_3 Nicholasp3_4 (terminase large subunit) Nicholasp3_5 (portal protein) Nicholasp3_6 (MuF-like minor capsid protein) Nicholasp3_7 (scaffolding protein) Nicholasp3_8 (major capsid protein) Nicholasp3_9 (head-to-tail adaptor) Nicholasp3_10 (head-to-tail stopper) Nicholasp3_11 Nicholasp3_12 (tail terminator) Nicholasp3_13 (major tail protein) Nicholasp3_14 (tail assembly chaperone) Nicholasp3_15 (tail assembly chaperone) Nicholasp3_16 (tape measure protein) Nicholasp3_17 (minor tail protein) Nicholasp3_18 (minor tail protein) Nicholasp3_19 (minor tail protein) Nicholasp3_20 (minor tail protein) Nicholasp3_21 (minor tail protein) Nicholasp3_22 (minor tail protein) Nicholasp3_23 Nicholasp3_24 (minor tail protein) Nicholasp3_25 Nicholasp3_26 Nicholasp3_27 (lysin A) Nicholasp3_28 (lysin B) Nicholasp3_29 Nicholasp3_30 Nicholasp3_31 Nicholasp3_32 (NrdH-like glutaredoxin) Nicholasp3_33 Nicholasp3_34 Nicholasp3_35 (nucleotidyltransferase) Nicholasp3_36 (Lsr2-like DNA bridging protein) Nicholasp3_37 Nicholasp3_38 (tyrosine integrase) Nicholasp3_39 Nicholasp3_40 (immunity repressor) Nicholasp3_41 (Cro protein) Nicholasp3_42 Nicholasp3_43 Nicholasp3_44 Nicholasp3_45 Nicholasp3_46 Nicholasp3_47 (esterase) Nicholasp3_48 Nicholasp3_49 Nicholasp3_50 Nicholasp3_51 Nicholasp3_52 Nicholasp3_53 Nicholasp3_54 Nicholasp3_55 (Ro-like RNA binding protein) Nicholasp3_56 Nicholasp3_57 Nicholasp3_58 Nicholasp3_59 Nicholasp3_60 Nicholasp3_61 Nicholasp3_62 Nicholasp3_63 (ERF family ssDNA binding protein) Nicholasp3_64 Nicholasp3_65 Nicholasp3_66 Nicholasp3_67 (HNH endonuclease) Nicholasp3_68 Nicholasp3_69 Nicholasp3_70 (DnaB-like dsDNA helicase) Nicholasp3_71 (WhiB family transcription factor) Nicholasp3_72 Nicholasp3_73 Nicholasp3_74 (phosphoribosyl pyrophosphate transferase) Nicholasp3_75 (nicotinate ribosyltransferase) Nicholasp3_76 (polynucleotide kinase) Nicholasp3_77 Nicholasp3_78 Nicholasp3_79 Nicholasp3_80 (RusA-like resolvase) Nicholasp3_81 (WhiB family transcription factor) Nicholasp3_82 (WhiB family transcription factor) Nicholasp3_83 Nicholasp3_84 Nicholasp3_85 (ssDNA-binding protein) Nicholasp3_86 Nicholasp3_87 Nicholasp3_88 (RNA ligase) Nicholasp3_89 Nicholasp3_90 (ClpP-like protease) Nicholasp3_91 Nicholasp3_92 Nicholasp3_93 Nicholasp3_94 Nicholasp3_95 (HNH endonuclease) Nicholasp3_96 Nicholasp3_97 Nicholasp3_98 (DnaQ-like DNA polymerase III subunit) Nicholasp3_99 Nicholasp3_100 Nicholasp3_101 Nicholasp3_102 Nicholasp3_103 Nicholasp3_104 Nicholasp3_105 Nicholasp3_107 Nicholasp3_111 Nicholasp3_117 Nicholasp3_118 Nicholasp3_120 Nicholasp3_121 Nicholasp3_123 Nicholasp3_125 Nicholasp3_126 Nicholasp3_127 (AAA-ATPase) Nicholasp3_128 Nicholasp3_129 Nicholasp3_130 Nicholasp3_131 Nicholasp3_132 Nicholasp3_133 Nicholasp3_134 Nicholasp3_135 Nicholasp3_136 Nicholasp3_137 Nicholasp3_138 Nicholasp3_139 Nicholasp3_140 Nicholasp3_141 Nicholasp3_142
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MF140422
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Not in Pitt Archives
SEA Designator	2012UTEPnicholasp3ZCRO
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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