| Detailed Information for Phage Patio |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Former names | TeaPea |
| Found By | Sarah Kuchan |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.442840, -79.956205 Map |
| Discovery Notes | -at time of soil collection it was a dry overcast day and was 26 F
-The soil was frozen
-the weather conditions over the last 24 hours were around 10 F and windy + overcast
-the soil was collected less than an hour before class (this is new)
-the soil had fertilizer pellets (blue dots) on it
|
| Naming Notes | Originally, I wanted to name him TP because I found him on the patio of one of our residence halls- Towers Patio. However, when I read the naming rules I saw the names could not be only 2 letters, so I extended it to TeePee (I still get the same effect). Then, that name was already taken. To combat this catastrophe, I changed it to TeaPea. Like the tea you drink + peas you eat. Very clever. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 30, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3811 |
| Genome length (bp) | 66251 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGCCGCGTAC |
| GC Content | 65.6% |
| Sequencing Notes | 22% of reads call 10 G's instead of consensus 9 between 65907 - 65917 bp |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CR |
| Subcluster | CR3 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | -temperate phage |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | -large plaques
-halos in all of them
-range from 1 mm- 3mm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919542 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
8.0x10^9 |
| Date of SEA Lysate Titering |
Feb 29, 2016 |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
F7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
