| Detailed Information for Phage Percastrophe | |
| Discovery Information | |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Former names | Percyverance |
| Found By | Percyval Seddoh |
| Year Found | 2017 |
| Location Found | Denton, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.19747 N, 97.132884 W Map |
| Discovery Notes | The soil was clumpy when it was obtained from a grassy area near bushes away from walking areas. The soil was dark and slightly moist. |
| Naming Notes | This was my 5th soil sample additionally, I adopted the phage from a classmate. For me the most important part of science can at times to catastrophic, and I had to learn this lesson. I had 4 failures prior to this phage. I fused my name "Percyval" with "catastrophe" to make "Percastrophe". |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 2, 2017 |
| Sequencing Facility | University of North Texas |
| Shotgun Sequencing Method | Illumina Sequencing |
| Genome length (bp) | 45999 |
| Character of genome ends | Unknown |
| Direct Terminal Repeat Length | 264 bp |
| End Determination Notes | While one end of the terminal repeat was obvious in the sequencing reads for this project, the other end was far less so. A buildup of reads was observed at a position that matched the right side of the terminal repeat in other similar phages, so that was selected as the right end of the terminal repeat. |
| GC Content | 59.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BF |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | My plaques are clear, circular, and approximately 3mm in diameter. |
| Morphotype | Podoviridae |
| Number of Genes | 64 |
| Number of tRNAs | 22 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPercastrophe_1 Percastrophe_2 Percastrophe_3 Percastrophe_4 Percastrophe_5 Percastrophe_6 Percastrophe_7 Percastrophe_8 Percastrophe_9 Percastrophe_10 Percastrophe_11 Percastrophe_12 Percastrophe_13 Percastrophe_36 Percastrophe_37 Percastrophe_38 Percastrophe_39 Percastrophe_40 Percastrophe_41 Percastrophe_42 Percastrophe_43 Percastrophe_44 Percastrophe_45 Percastrophe_46 Percastrophe_47 Percastrophe_48 Percastrophe_49 Percastrophe_50 Percastrophe_51 Percastrophe_52 Percastrophe_53 Percastrophe_54 Percastrophe_55 Percastrophe_56 Percastrophe_57 Percastrophe_58 Percastrophe_59 Percastrophe_60 Percastrophe_61 Percastrophe_62 Percastrophe_63 Percastrophe_64 Percastrophe_65 Percastrophe_66 Percastrophe_67 Percastrophe_68 Percastrophe_69 Percastrophe_70 Percastrophe_71 Percastrophe_72 Percastrophe_73 Percastrophe_74 Percastrophe_75 Percastrophe_76 Percastrophe_77 Percastrophe_78 Percastrophe_79 Percastrophe_80 Percastrophe_81 Percastrophe_82 Percastrophe_83 Percastrophe_84 Percastrophe_85 Percastrophe_86 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG663583 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 4.0 x 10^9 |
| Pitt Freezer Box# | 70 |
| Pitt Freezer Box Grid# | D2 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
