Streptomyces phage Percastrophe
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Detailed Information for Phage Percastrophe
Discovery Information
Isolation HostStreptomyces griseus ATCC 10137
Former namesPercyverance
Found ByPercyval Seddoh
Year Found2017
Location FoundDenton, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates33.19747 N, 97.132884 W Map
Discovery NotesThe soil was clumpy when it was obtained from a grassy area near bushes away from walking areas. The soil was dark and slightly moist.
Naming NotesThis was my 5th soil sample additionally, I adopted the phage from a classmate. For me the most important part of science can at times to catastrophic, and I had to learn this lesson. I had 4 failures prior to this phage. I fused my name "Percyval" with "catastrophe" to make "Percastrophe".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 2, 2017
Sequencing FacilityUniversity of North Texas
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage279
Genome length (bp)45999
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length264 bp
End Determination NotesWhile one end of the terminal repeat was obvious in the sequencing reads for this project, the other end was far less so. A buildup of reads was observed at a position that matched the right side of the terminal repeat in other similar phages, so that was selected as the right end of the terminal repeat.
GC Content59.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBF
Subcluster--
Other Cluster Members
Plaque NotesMy plaques are clear, circular, and approximately 3mm in diameter.
Final annotation complete?No
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload