Microbacterium phage Pioneer3
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Detailed Information for Phage Pioneer3
Discovery Information
Isolation HostMicrobacterium paraoxydans NWU1
Found ByMorgan A. Shipley
Year Found2016
Location FoundLincoln, NE United States
Finding InstitutionNebraska Wesleyan University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates40.8 N, 96.6 W Map
Discovery NotesThe environmental sample was from a tree berm outside of a dormitory at Nebraska Wesleyan University. This sample produced the phage that completed the program.
Naming NotesI named this phage Pioneer3 because it is the name of the dormitory I live in, as well as where my original environmental sample came from to produce my phage, and 3 is my lucky number.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 25, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1255
Genome length (bp)62954
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length3191 bp
GC Content64.6%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 5/15/2018)
Plaque NotesThe plaques had long tails, making them siphoviridae. The heads were very dark in the Electron Microscopy image, meaning that they were packed with DNA from the high concentration.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.3x10^10
Date of SEA Lysate Titering Nov 8, 2016
Pitt Freezer Box# 45
Pitt Freezer Box Grid# F9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload