Mycobacterium phage Pipsqueaks
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Detailed Information for Phage Pipsqueaks
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByVictoria Edmund and Taylor Gibson
Year Found2014
Location FoundCharleston, SC USA
Finding InstitutionCollege of Charleston
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates34.783667 N, 79.939806 W Map
Discovery NotesI chose to take a sample from a garden behind Addlestone Library. The soil sample was moist, soft, and very dark.
Naming NotesOur phage had very small plaques; therefore, we named our phage Pipsqueaks.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 11, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4988
Genome length (bp)43679
Character of genome ends3' Sticky Overhang
Overhang Length14 bases
Overhang SequenceCCCGCCGCAATGGG
GC Content66.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterN
Subcluster--
Other Cluster Members
MorphotypeSiphoviridae
Final annotation complete?Yes
Number of Genes73
Has been Phamerated?Yes
Gene List

Click to View

Pipsqueaks_1

Pipsqueaks_2
(terminase)

Pipsqueaks_3

Pipsqueaks_4
(portal)

Pipsqueaks_5
(capsid maturation protease)

Pipsqueaks_6
(major capsid)

Pipsqueaks_7
(head-to-tail-connector)

Pipsqueaks_8
(head-to-tail-connector)

Pipsqueaks_9
(head-to-tail-connector)

Pipsqueaks_10
(head-to-tail-connector)

Pipsqueaks_11
(head-to-tail-connector)

Pipsqueaks_12
(head-to-tail-connector)

Pipsqueaks_13
(Major Tail)

Pipsqueaks_14
(Tail Assembly Chaperone)

Pipsqueaks_15
(Tail Assembly Chaperone)

Pipsqueaks_16
(tape measure)

Pipsqueaks_17
(minor tail)

Pipsqueaks_18
(minor tail)

Pipsqueaks_19
(minor tail)

Pipsqueaks_20
(minor tail)

Pipsqueaks_21

Pipsqueaks_22
(minor tail)

Pipsqueaks_23

Pipsqueaks_24

Pipsqueaks_25

Pipsqueaks_26

Pipsqueaks_27

Pipsqueaks_28
(lysin A)

Pipsqueaks_29
(holin)

Pipsqueaks_30

Pipsqueaks_31

Pipsqueaks_32

Pipsqueaks_33

Pipsqueaks_34

Pipsqueaks_35

Pipsqueaks_36
(integrase (Y-int))

Pipsqueaks_37
(immunity repressor)

Pipsqueaks_38
(excise)

Pipsqueaks_39

Pipsqueaks_40

Pipsqueaks_41

Pipsqueaks_42

Pipsqueaks_43

Pipsqueaks_44

Pipsqueaks_45
(WhiB family transcription factor)

Pipsqueaks_46

Pipsqueaks_47
(RecE exonuclease)

Pipsqueaks_48
(RecT ssDNA binding protein)

Pipsqueaks_49
(DNA binding, putative HJC)

Pipsqueaks_50
(NrdH glutaredoxin)

Pipsqueaks_51

Pipsqueaks_52

Pipsqueaks_53

Pipsqueaks_54

Pipsqueaks_55

Pipsqueaks_56

Pipsqueaks_57

Pipsqueaks_58

Pipsqueaks_59

Pipsqueaks_60

Pipsqueaks_61

Pipsqueaks_62
(HNH Endonuclease)

Pipsqueaks_63

Pipsqueaks_64

Pipsqueaks_65

Pipsqueaks_66

Pipsqueaks_67

Pipsqueaks_68

Pipsqueaks_69

Pipsqueaks_70

Pipsqueaks_71

Pipsqueaks_72

Pipsqueaks_73
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKU935730
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload