Arthrobacter phage Polka
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Detailed Information for Phage Polka
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByAudrey Jonas
Year Found2017
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.44485 N, 79.95301 W Map
Discovery NotesSoil was found around some broken sidewalk concrete. Dark gray in color and the consistency of wet sand.
Naming NotesMy best friend was a gym class polka champion in high school and after he told me the story I knew it had to be documented.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 11, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage642
Genome length (bp)38077
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceGAGTTGCCGGCA
GC Content66.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAS
SubclusterAS2
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque Notes1.5 mm clear plaques with cloudy edges
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Polka_1
(terminase, large subunit (ATPase domain))

Polka_2
(terminase, large subunit (DNA binding domain))

Polka_3
(terminase, large subunit (nuclease domain))

Polka_4
(RNA binding protein)

Polka_5
(portal protein)

Polka_6
(capsid maturation protease)

Polka_7
(major capsid protein)

Polka_8

Polka_9
(head-to-tail adaptor)

Polka_10
(head-to-tail stopper)

Polka_11

Polka_12
(tail terminator)

Polka_13
(major tail protein)

Polka_14
(tail assembly chaperone)

Polka_15
(tail assembly chaperone)

Polka_16
(tape measure protein)

Polka_17
(minor tail protein)

Polka_18
(minor tail protein)

Polka_19
(minor tail protein)

Polka_20
(minor tail protein)

Polka_21
(membrane protein)

Polka_22

Polka_23
(lysin A)

Polka_24
(membrane protein)

Polka_25

Polka_26

Polka_27

Polka_28

Polka_29

Polka_30

Polka_31
(HNH endonuclease)

Polka_32
(helix-turn-helix DNA binding protein)

Polka_33

Polka_34

Polka_35

Polka_36
(integrase, (Y-int))

Polka_37
(immunity repressor)

Polka_38
(helix-turn-helix DNA binding protein)

Polka_39
(excise)

Polka_40
(RecE-like exonuclease)

Polka_41
(RecT-like ssDNA binding protein)

Polka_42
(helix-turn-helix DNA binding protein)

Polka_43
(RusA-like resolvase (endonuclease))

Polka_44
(DNA binding protein)

Polka_45
(DNA methlyase)

Polka_46
(DNA methylase)

Polka_47

Polka_48

Polka_49

Polka_50

Polka_51
(2 TMH)

Polka_52
(Had VERY weak hits to toxin)

Polka_53
(deoxycytidylate deaminase)

Polka_54

Polka_55
(endonuclease)

Polka_56
(terminase, small subunit)

Polka_57

Polka_58

Polka_59

Polka_60

Polka_61

Polka_62

Polka_63

Polka_64

Polka_65

Polka_66

Polka_67

Polka_68
(membrane protein)

Polka_69

Polka_70
(helix-turn-helix DNA binding protein)

Polka_71
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH834624
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 6
Pitt Freezer Box Grid# B3
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload