Mycobacterium phage Rolipoli
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| Detailed Information for Phage Rolipoli | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Zion McCoy |
| Year Found | 2017 |
| Location Found | Washington , DC USA |
| Finding Institution | Howard University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.9197 N, 77.0187 W Map |
| Discovery Notes | RoliPoli was discovered on the grounds of the Administrative building at the Illustrious Howard University. Researches dug about 7 cm into the top soil to extrude RoliPoli from the soil. |
| Naming Notes | The name RoliPoli came from this phage's constant activity. After numerous times of plating and very concentrated dilutions, I was finally able to get plaques from this phage that did not completely lyse the plate. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | University of Pittsburgh Genomics and Proteomics Core Laboratories |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | RoliPoli is a very clear phage which means he is lytic. There is no cloudiness in the plaques that he produces. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | RoliPoli is of only one morphology. Roli Poli had to go through three rounds of serial dilutions in order to purify him well enough to produce only one morphology of plaque. |
| Morphotype | Myoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 3.8 X 10^10 |
| Pitt Freezer Box# | 53 |
| Pitt Freezer Box Grid# | E4 |