The Actinobacteriophage Database

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Detailed Information for Phage RonRayGun
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Seth Boyd
Year Found	2012
Location Found	Arkadelphia, AR USA
Finding Institution	Ouachita Baptist University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	No
Isolation Temperature	Not entered
GPS Coordinates	34.125144 N, 93.052808 W Map
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Feb 4, 2013
Sequencing Facility	Virginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method	454 Pyrosequencing
Approximate Shotgun Coverage	460
Sanger Finishing Reactions	2
Genome length (bp)	42596
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	CGAAACGCCAT
GC Content	66.1%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	T
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Bernal13 Ibrahim Mendokysei Nairb RonRayGun Whitty ZenTime222
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	small, cloudy plaques meaning temperate phage.
Morphotype	Siphoviridae
Number of Genes	61
Has been Phamerated?	Yes
Gene List	Click to View RonRayGun_1 RonRayGun_2 (terminase small subunit) RonRayGun_3 (terminase) RonRayGun_4 (portal protein) RonRayGun_5 (MuF-like minor capsid protein) RonRayGun_6 (helix-turn-helix DNA binding protein) RonRayGun_7 RonRayGun_8 (major capsid protein) RonRayGun_9 RonRayGun_10 RonRayGun_11 RonRayGun_12 RonRayGun_13 RonRayGun_14 (major tail protein) RonRayGun_15 RonRayGun_16 RonRayGun_17 (tape measure protein) RonRayGun_18 (minor tail protein) RonRayGun_19 (minor tail protein) RonRayGun_20 RonRayGun_21 RonRayGun_22 (minor tail protein) RonRayGun_23 RonRayGun_24 RonRayGun_25 RonRayGun_26 RonRayGun_27 (lysin A) RonRayGun_28 (holin) RonRayGun_29 RonRayGun_30 RonRayGun_31 RonRayGun_32 RonRayGun_33 (tyrosine integrase) RonRayGun_34 (immunity repressor) RonRayGun_35 (MPME2 protein) RonRayGun_36 (helix-turn-helix DNA binding protein) RonRayGun_37 RonRayGun_38 RonRayGun_39 RonRayGun_40 RonRayGun_41 (WhiB family transcription factor) RonRayGun_42 RonRayGun_43 RonRayGun_44 (RecB-like exonuclease/helicase) RonRayGun_45 RonRayGun_46 RonRayGun_47 RonRayGun_48 RonRayGun_49 (helix-turn-helix DNA binding protein) RonRayGun_50 RonRayGun_51 RonRayGun_52 RonRayGun_53 RonRayGun_54 RonRayGun_55 RonRayGun_56 RonRayGun_57 RonRayGun_58 RonRayGun_59 (HNH endonuclease) RonRayGun_60 (DNA methylase) RonRayGun_61
Submitted Annotation in DNA Master Format	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KM591905
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	38
Pitt Freezer Box Grid#	44
Available Files
Plaque Picture	Download

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