| Detailed Information for Phage Sadboi | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Michael Lauer |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.447276 N, 79.953995 W Map |
| Discovery Notes | found in a bed of flowers at the University of Pittsburgh outside of Crawford Hall. Each sample in the series was taken from different holes in the flowerbed. |
| Naming Notes | A catch phrase that I coined when you're having a depressing day; you are sadboi for the day. It is also used to reference people born in 1998; they are sadboi_98. I hope that this phage has 98 genes so that there will be a sadboi_98 gene. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 24, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 711 |
| Genome length (bp) | 67485 |
| Character of genome ends | Circularly Permuted |
| GC Content | 58.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DV |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | Lysate diluted and spotted, no mesas after 5 days. The center of the lytic clearings were picked and steaked on a PYCa plate in a patch test, and no lysogenic cells grew. |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | lytic, small, pinhole-like |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewSadboi_1 Sadboi_2 Sadboi_3 Sadboi_4 Sadboi_5 Sadboi_6 Sadboi_7 Sadboi_8 Sadboi_9 Sadboi_10 Sadboi_11 Sadboi_12 Sadboi_13 Sadboi_14 Sadboi_15 Sadboi_16 Sadboi_17 Sadboi_18 Sadboi_19 Sadboi_20 Sadboi_21 Sadboi_22 Sadboi_23 Sadboi_24 Sadboi_25 Sadboi_26 Sadboi_27 Sadboi_28 Sadboi_29 Sadboi_30 Sadboi_31 Sadboi_32 Sadboi_33 Sadboi_34 Sadboi_35 Sadboi_36 Sadboi_37 Sadboi_38 Sadboi_39 Sadboi_40 Sadboi_41 Sadboi_42 Sadboi_43 Sadboi_44 Sadboi_45 Sadboi_46 Sadboi_47 Sadboi_48 Sadboi_49 Sadboi_50 Sadboi_51 Sadboi_52 Sadboi_53 Sadboi_54 Sadboi_55 Sadboi_56 Sadboi_57 Sadboi_58 Sadboi_59 Sadboi_60 Sadboi_61 Sadboi_62 Sadboi_63 Sadboi_64 Sadboi_65 Sadboi_66 Sadboi_67 Sadboi_68 Sadboi_69 Sadboi_70 Sadboi_71 Sadboi_72 Sadboi_73 Sadboi_74 Sadboi_75 Sadboi_76 Sadboi_77 Sadboi_78 Sadboi_79 Sadboi_80 Sadboi_81 Sadboi_82 Sadboi_83 Sadboi_84 Sadboi_85 Sadboi_86 Sadboi_87 Sadboi_88 Sadboi_89 Sadboi_90 Sadboi_91 Sadboi_92 Sadboi_93 Sadboi_94 Sadboi_95 Sadboi_96 Sadboi_97 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN234232 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.0X10^11 pfu/mL |
| Pitt Freezer Box# | 96 |
| Pitt Freezer Box Grid# | E8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
