| Detailed Information for Phage Sassafras |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Joshua Kammeraad |
| Year Found | 2010 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.789083 N, 86.104138 W Map |
| Discovery Notes | Soil saturated with water, sample taken at a depth of 5cm, 22 degrees C, urban setting, flat |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 13, 2020 |
| Sequencing Facility | Hope College |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 123 |
| Genome length (bp) | 57051 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Sassafras only produces one plaque morphology. Plaque diameters are only 1-2mm after 24 hours, but plaques grow steadily to over 1cm in diameter after a week of incubation. Low turbidity after 24 hours but then turbidity begins to range in a bulls-eye pattern that becomes more and then less turbid with distance from center. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW862989 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2010HOPEsassafrasJAK |
| Pitt Freezer Box# |
14 |
| Pitt Freezer Box Grid# |
30 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
