Mycobacterium phage SchoolBus
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Detailed Information for Phage SchoolBus
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeam Afplaq
Year Found2017
Location FoundSanta Monica, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature35°C
GPS Coordinates34.031111 N, 118.473333 W Map
Discovery NotesPhage SchoolBus was isolated from soil, collected on 10/07/17 by Kyle Scranton,
at a depth of about 2 inches from soil taken under a park bench near a trash can. This sample was mostly dry soil w/ small pieces of wood. Air temp at time of collection was 26.7˚C.

SchoolBus was isolated by direct plating at 35C.

Characterization was performed by Team Afplaq (Jason Eigenbrood, Nima Hooshdaran, Kyle Scranton).

Phage morphology under EM shows siphoviridae with prolate head characteristic of cluster O phages.

Midi-Lysate (Archive) Titer: 1.6 x 1014
Date of High-titer Lysate Titering: 11/08/17

MIMG103AL-F17 Instructor: Dr. Amanda Freise
Teaching Assistant: Andrew Lund
Undergraduate Assistant: Keith Mitchell
Laboratory Support: Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker
Naming NotesWe decided to name our phage SchoolBus because it looked like a school bus to our team.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 21, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1254
Genome length (bp)71651
Character of genome endsUnknown
Overhang Length4 bases
Overhang SequenceGTGT
GC Content65.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterO
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesClear
Average size (and size range) diameter in mm: Average=1.0mm; Range=0.75mm-1.25mm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

SchoolBus_1

SchoolBus_2

SchoolBus_3

SchoolBus_4

SchoolBus_5
(DNA methylase)

SchoolBus_6
(DNA methylase)

SchoolBus_7

SchoolBus_8

SchoolBus_9
(endonuclease VII)

SchoolBus_10

SchoolBus_11

SchoolBus_12
(exonuclease)

SchoolBus_13

SchoolBus_14

SchoolBus_15

SchoolBus_16

SchoolBus_17

SchoolBus_18

SchoolBus_19
(DNA primase/polymerase)

SchoolBus_20

SchoolBus_21

SchoolBus_22
(MPME 1 protein)

SchoolBus_23
(WhiB family transcription factor)

SchoolBus_24

SchoolBus_25

SchoolBus_26

SchoolBus_27
(HNH endonuclease)

SchoolBus_28

SchoolBus_29

SchoolBus_30
(terminase large subunit)

SchoolBus_31
(HNH endonuclease)

SchoolBus_32
(portal protein)

SchoolBus_33
(O-methyltransferase)

SchoolBus_34
(glycosyltransferase)

SchoolBus_35
(glycosyltransferase)

SchoolBus_36
(glycosyltransferase)

SchoolBus_37
(capsid maturation protease)

SchoolBus_38

SchoolBus_39
(major capsid protein)

SchoolBus_40

SchoolBus_41
(head-to-tail connector complex protein)

SchoolBus_42

SchoolBus_43
(head-to-tail connector complex protein)

SchoolBus_44

SchoolBus_45

SchoolBus_46
(head-to-tail connector complex protein)

SchoolBus_47
(major tail protein)

SchoolBus_48

SchoolBus_49

SchoolBus_50

SchoolBus_51
(helix-turn-helix DNA binding domain protein)

SchoolBus_52
(tail assembly chaperone)

SchoolBus_53
(tail assembly chaperone)

SchoolBus_54
(tape measure protein)

SchoolBus_55
(minor tail protein)

SchoolBus_56
(minor tail protein)

SchoolBus_57
(minor tail protein)

SchoolBus_58
(minor tail protein)

SchoolBus_59
(minor tail protein)

SchoolBus_60
(minor tail protein)

SchoolBus_61
(minor tail protein)

SchoolBus_62

SchoolBus_63

SchoolBus_64
(minor tail protein)

SchoolBus_65

SchoolBus_66
(lysin A)

SchoolBus_67
(lysin B)

SchoolBus_68
(holin)

SchoolBus_69

SchoolBus_70

SchoolBus_71

SchoolBus_72

SchoolBus_73
(helix-turn-helix DNA binding domain protein)

SchoolBus_74

SchoolBus_75

SchoolBus_76

SchoolBus_77

SchoolBus_78

SchoolBus_79

SchoolBus_80
(DNA polymerase III sliding clamp beta)

SchoolBus_81
(band-7-like membrane protein)

SchoolBus_82

SchoolBus_83

SchoolBus_84

SchoolBus_85
(helix-turn-helix DNA binding domain protein)

SchoolBus_86
(dsDNA break-binding protein)

SchoolBus_87

SchoolBus_88

SchoolBus_89
(dsDNA partitioning protein)

SchoolBus_90

SchoolBus_91

SchoolBus_92

SchoolBus_93

SchoolBus_94

SchoolBus_95
(AAA-ATPase)

SchoolBus_96

SchoolBus_97

SchoolBus_98

SchoolBus_99

SchoolBus_100

SchoolBus_101

SchoolBus_102

SchoolBus_103

SchoolBus_104

SchoolBus_105

SchoolBus_106
(helix-turn-helix DNA binding domain protein)

SchoolBus_107

SchoolBus_108

SchoolBus_109

SchoolBus_110

SchoolBus_111

SchoolBus_112

SchoolBus_113

SchoolBus_114

SchoolBus_115

SchoolBus_116

SchoolBus_117

SchoolBus_118

SchoolBus_119

SchoolBus_120

SchoolBus_121

SchoolBus_122

SchoolBus_123

SchoolBus_124

SchoolBus_125

SchoolBus_126

SchoolBus_127

SchoolBus_128

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH020246
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 58
Pitt Freezer Box Grid# F7
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload