Mycobacterium phage SchoolBus
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Detailed Information for Phage SchoolBus
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeam Afplaq
Year Found2017
Location FoundSanta Monica, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature35°C
GPS Coordinates34.031111 N, 118.473333 W Map
Discovery NotesPhage SchoolBus was isolated from soil, collected on 10/07/17 by Kyle Scranton,
at a depth of about 2 inches from soil taken under a park bench near a trash can. This sample was mostly dry soil w/ small pieces of wood. Air temp at time of collection was 26.7˚C.

SchoolBus was isolated by direct plating at 35C.

Characterization was performed by Team Afplaq (Jason Eigenbrood, Nima Hooshdaran, Kyle Scranton).

Phage morphology under EM shows siphoviridae with prolate head characteristic of cluster O phages.

Midi-Lysate (Archive) Titer: 1.6 x 1014
Date of High-titer Lysate Titering: 11/08/17

MIMG103AL-F17 Instructor: Dr. Amanda Freise
Teaching Assistant: Andrew Lund
Undergraduate Assistant: Keith Mitchell
Laboratory Support: Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker
Naming NotesWe decided to name our phage SchoolBus because it looked like a school bus to our team.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 21, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Genome length (bp)71651
Character of genome endsUnknown
Overhang Length4 bases
Overhang SequenceGTGT
GC Content65.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterO
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 5/15/2018)
Plaque NotesClear
Average size (and size range) diameter in mm: Average=1.0mm; Range=0.75mm-1.25mm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
EM PictureDownload