Mycobacterium phage Sisyphus
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| Detailed Information for Phage Sisyphus | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Natalie Sargent & Quinn Beato |
| Year Found | 2021 |
| Location Found | Boulder, CO United States |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 31°C |
| GPS Coordinates | 40.009123 N, 105.269114 W Map |
| Discovery Notes | Found under a tree near the base of a tree where there was dampened soil outside of the Porter biosciences building in the University of Colorado, Boulder. Climate is arid. The soil was found September 8th 2021 around 10:20 in the morning. |
| Naming Notes | Sisyphus was a Greek king who was punished by Hades to eternally roll a Boulder up a hill every day only to have it rumble back down before it could reach the top. Much like Sisyphus this phage was stubborn with purification and quality control gels and we kept having to use titer plates at lower and lower dilutions. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques are 3-4mm in diameter and clear meaning the phage is lytic. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.84e7 |
| Pitt Freezer Box# | 142 |
| Pitt Freezer Box Grid# | A12 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
