Mycobacterium phage SkinnyMini
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| Detailed Information for Phage SkinnyMini | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Timothy |
| Found By | Lydia Buzzard |
| Year Found | 2013 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | Unavailable |
| Discovery Notes | I collected my soil sample from dry topsoil outside the EBS building on Western Kentucky University's campus. The area is grassy, receives little foot traffic, and is surrounded by trees and other foliage. At the time of collection, the soil was covered with leaves. I believe that my phage is an A4 phage. I reached this conclusion after comparing pictures of my gel from gel electrophoresis with other gels in the database. My gel pattern best resembles those of other sequenced A4 phages. However, I cannot know for sure which cluster my phage belongs to without sequencing its DNA. There are no plans to sequence SkinnyMini's DNA at this point. |
| Naming Notes | I named this phage SkinnyMini because its plaques were so small compared to those of my classmates' phages. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | I believe that my phage is temperate based on the turbidity of its plaques. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques are very small (about 2mm in diameter or less) and turbid. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2013WEKUSkinnyMiniLMB |
| SEA Lysate Titer | 1.57x10^9pfu/mL |
| Date of SEA Lysate Titering | Oct 18, 2013 |
| Pitt Freezer Box# | 5 |
| Pitt Freezer Box Grid# | F2 |