Mycobacterium phage Sneeze
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Detailed Information for Phage Sneeze
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMarina Grossi
Year Found2014
Location FoundPalo Verde, Costa Rica
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates10.4 N, 85.32 W Map
Discovery NotesI "adopted" Sneeze from Joseph Skibbens, a member of my SEA Phages course. Since I consistently did not get plaques on any of my plates, I obtained a plaque from one of Joseph's plates that had two plaques with very different morphologies. At the time the soil was collected in early January, the temperature was 85 degrees Fahrenheit and it was taken at a depth of 0.5". It was collected from a recently dried-up marsh.
Naming NotesI named Sneeze after the nickname my family gave to my dog.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 10, 2014
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage3162
Genome length (bp)42429
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTCCCATGGCAT
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterG
SubclusterG1
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe plaques are usually 1-2 mm in diameter, with turbid (but not very cloudy) centers. These plaques are surrounded by a very turbid "halo" that can easily be seen when held up to a light source. When left in the incubator, these "halos" can grow in diameter (about 1 mm after the 72 hour test).
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX534004
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.00x10^10 pfu/ml
Date of SEA Lysate Titering Mar 12, 2014
Pitt Freezer Box# 7
Pitt Freezer Box Grid# B1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload