Arthrobacter phage SorJuana
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Detailed Information for Phage SorJuana
Discovery Information
Isolation HostArthrobacter sp. ATCC 21022
Found ByTrevor Cross
Year Found2014
Location FoundRoyersford, PA USA
Finding InstitutionCabrini University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.209448 N, 75.559227 W Map
Discovery NotesFound during the winter under a section of frozen water from the Schuylkill River.


Please note that phage lysate was made with glycerol for archiving purposes.
Naming NotesSor Juana was a nun during the 17th century in colonial Mexico who was known for her poetry and intellectual prowess.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 17, 2014
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage348
Genome length (bp)58979
Character of genome endsDirect Terminal Repeat
GC Content53.0%
Sequencing NotesThis phage is Amigo-like, and thus has the same pattern of ends. There's a terminal redundancy where one end is clearly defined (the right end as the genome is canonically oriented) and the other end is less clear. The left end was chosen to match Amigo's left end, and also matches the approximate boundary of the region of double coverage in the SorJuana sequencing data.

With the chosen end, the terminal repeat is 1589 bp, though this is approximate.
Fasta file available?Yes: Download fasta file
Characterization
ClusterAQ
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notes5 mm mostly clear plaques after 24 hours at 30⁰C on 4.5 mM CaCl2
MorphotypeSiphoviridae
Number of Genes86
Number of tRNAs7
Has been Phamerated?Yes
Gene List

Click to View

SorJuana_1

SorJuana_2

SorJuana_3

SorJuana_4

SorJuana_5

SorJuana_6

SorJuana_7

SorJuana_8

SorJuana_9

SorJuana_10

SorJuana_11

SorJuana_17

SorJuana_18
(minor tail protein)

SorJuana_19
(minor tail protein)

SorJuana_20

SorJuana_21

SorJuana_22
(terminase)

SorJuana_23
(portal protein)

SorJuana_24
(capsid maturation protease)

SorJuana_25

SorJuana_26
(major capsid protein)

SorJuana_27

SorJuana_28

SorJuana_29

SorJuana_30

SorJuana_31

SorJuana_32

SorJuana_33

SorJuana_34
(tail assembly chaperone)

SorJuana_35
(tail assembly chaperone)

SorJuana_36
(tape measure protein)

SorJuana_37
(minor tail protein)

SorJuana_38
(minor tail protein)

SorJuana_39

SorJuana_40

SorJuana_41

SorJuana_42
(endolysin)

SorJuana_43

SorJuana_44

SorJuana_45

SorJuana_46
(DnaQ-like DNA polymerase III subunit)

SorJuana_47

SorJuana_48

SorJuana_49

SorJuana_50

SorJuana_51
(RuvC-like resolvase)

SorJuana_52

SorJuana_53

SorJuana_54
(DNA polymerase I)

SorJuana_55
(transposase)

SorJuana_56
(DNA primase)

SorJuana_59

SorJuana_60
(RecA-like DNA recombinase)

SorJuana_61
(Cas4 family exonuclease)

SorJuana_62
(WhiB family transcription factor)

SorJuana_63

SorJuana_64
(polynucleotide kinase)

SorJuana_65
(RNA ligase)

SorJuana_66

SorJuana_67

SorJuana_68

SorJuana_69

SorJuana_70
(DNA helicase)

SorJuana_71

SorJuana_72

SorJuana_73

SorJuana_74

SorJuana_75
(DNA methyltransferase)

SorJuana_76
(MazG-like nucleotide pyrophosphohydrolase)

SorJuana_77

SorJuana_78

SorJuana_79

SorJuana_80

SorJuana_81

SorJuana_82

SorJuana_83

SorJuana_84

SorJuana_85

SorJuana_86

SorJuana_87

SorJuana_88

SorJuana_89

SorJuana_90

SorJuana_91

SorJuana_92

SorJuana_93

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKU160666
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 149
Pitt Freezer Box Grid# B4
Available Files
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload