| Detailed Information for Phage StCroix |
| Discovery Information |
| Isolation Host | Rhodococcus erythropolis RIA 643 |
| Found By | Bradley Koch and Brittany Lubich |
| Year Found | 2014 |
| Location Found | Hudson, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 44.979117 N, 92.759906 W Map |
| Discovery Notes | from same soil sample as Naiad, east bank of St Croix River, near kayak launch site, next to stream running from river to walking path; very wet soil with decayed plant material |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 21, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Genome length (bp) | 46619 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGTGAT |
| GC Content | 58.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CA |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | turbid plaques appearing after 48 hr culture at 30oC; separated from clear plaques in same sample (a clear plaque was isolated as Naiad) |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF324900 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.7 x 10^8 |
| Date of SEA Lysate Titering |
Dec 17, 2014 |
| Pitt Freezer Box# |
12 |
| Pitt Freezer Box Grid# |
H5 |
| Available Files |
| GenBank File for Phamerator | Download |