Gordonia phage Stormie
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| Detailed Information for Phage Stormie | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Leah N Dickey |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 27.164 N, 57.373 W Map |
| Discovery Notes | Discovered on Jan. 13, 2016 outside of a friend's house underneath a dead bush. 24 hours previous to the soil collection the weather was partly cloudy and windy with moderate-to-heavy snowfall and temperatures ~20 degrees F and below. It was 7 degrees F and sunny when I collected Stormie's soil sample. I used a clean plastic spoon to transer ~ 1/2 cup of soil into a clean, unused plastic bag. The soil was dry, cold, clumpy, malleable, and thick with roots, dead leaves, twigs, and of course, snow. The soil sample was stored in my backpack at room temperature (~70 deg F) for about one hour before enrichment. |
| Naming Notes | I named my phage Stormie because I collected her soil sample right after a snowstorm hit the area. I doubted Stormie's capabilities and had no hope in finding a phage in this sample, but here she is. Stormie even held her ground when a contaminant made its way into the enrichment spot test and tried to take over her plaque. She's obviously the most fierce phage you'll ever find. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | When I purified the smallest morphologies I checked the plates twice: after 48 hours and after 5 days. 48 hours post incubation the 10^0 and 10^ -1 plates were cleared, and there were barely any Gordonia terrae host cells on the webbed plate. 5 days post incubation the 10^0 and 10^-1 plates had orange Gordonia host cells to the point where it looked like I poured a bacteria lawn without introducing it to a phage. The webbed plate looked completely different--there was still about the same amount of Gordonia cells present that I observed 48 hours post, however the plaques looked smaller and there wasn't any Gordonia separating them. So, it seems as though Stormie may go through the lytic AND the lysogenic cycle. Stormie keeps getting more and more complex as my research unfolds--amazing! |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Stormie actually has two unique morphologies. The first morphology consists of extremely small plaques while the other morphology consists of small, yet significantly larger plaques. Both morphologies were separately purified and the results were the same for both. Please refer to the image provided to see what Stormie's morphologies look like! |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 3 |
| Pitt Freezer Box Grid# | H1 |
| Available Files | |
| Plaque Picture | Download |
