| Detailed Information for Phage Tangent |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Tanvi Shah |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.444221 N, 79.954441 W Map |
| Discovery Notes | Collected January 8th, 2017
Temperature was about 10 degress Fahrenheit
Dirt was very hard, partially frozen, and chunky
Collected from the bus stop outside of the Cathedral of Learning |
| Naming Notes | The name for this phage comes from my high school nickname. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 16, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2425 |
| Genome length (bp) | 58281 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DE |
| Subcluster | DE1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques about 0.25 -0.50 mm in diameter
Blurry edges (most likely caused by lysis of cells, not directly by phage particles)
Plaques round and slightly oblong (not perfectly circular)
Plaque morphology image from a 10^-3 serial dilution |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH976519 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
56 |
| Pitt Freezer Box Grid# |
E6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
