Mycobacterium phage Thumb
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Detailed Information for Phage Thumb
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesThom3001
Found ByAna Garcia
Year Found2017
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates34.0732 N, 118.4505 W Map
Discovery NotesPhage Thom3001 was isolated from soil was collected from the ground (depth of 1 in). Taken from moist garden area from the "Hill" at UCLA. In close proximity to buildings and walkways. Phage was isolated via Direct plating at 30C.

Characterization ws performed by Team Legendary Lysis (Brian Aguirre, Ana Garcia, Juhee Lim, Malcolm Phung) and Team Drop it Lytics Hot (Zilla Wentworth, Paola Nol Bernardino, Joel Laskow, Carlos Rojas).

Name of Individual who collected sample: Ana Garcia
Midi-Lysate (Archive) Titer: 1 x 10^11
Date of Midi-Lysate Titer: 5-11-2017

MIMG103AL-S17 Instructor: Dr. Jordan Moberg Parker
Teaching Assistant: Ximena Ibarra
Undergraduate Assistant: Amara Pang
Laboratory Support: Dr. Krisanavane Reddi
Naming NotesThis phage was named Thumb because it sounds kind of like the previously rejected name and the instructor thought it was funny.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 10, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Genome length (bp)68408
Character of genome endsUnknown
GC Content57.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterH
SubclusterH1
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque Notes1mm clear
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG962376
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 45
Pitt Freezer Box Grid# D5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload