Mycobacterium phage TookTime
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| Detailed Information for Phage TookTime | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Svetlana Lazutkin, Dawna Montgomery |
| Year Found | 2017 |
| Location Found | Farmersville, TX USA |
| Finding Institution | Collin College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 21°C |
| GPS Coordinates | 33.171 N, 96.3627 W Map |
| Discovery Notes | The sample was collected at 8:15am, 08.30.17. The weather was partly cloudy. The soil was dry on the surface, slightly damp & compact at depth of 6 inches below surface. The spot was surrounded by a private fence on the east border, by a tree and firewood pile on the north border. The west border was a drop off in the yard with little grass.The south border was a garden with jalapeño plants. |
| Naming Notes | It took us a few times. So we choose the name TookTime for the phage name. Also, the word is an onomatopoeia, an imitation of sound of a clock: took time, took time, too time... |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | TookTime is a siphoviridae virus with an icosahedral capsid approximately 61 nm in diameter and a long flexible tail 200 nm long. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques were almost perfectly circular, with entire margins, clear, similar to each other in shape but slightly diverse in size. The largest plaques were 1.9mm in diameter. They reached this size when there was plenty of space to grow. In crowded conditions, the plagues were 0.5 mm and less. We counted 82 plaques total in the plate from dilution 10^-6 of a sample from the collected lysate. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 3.2X10^8 pfu/ml |
| Pitt Freezer Box# | 66 |
| Pitt Freezer Box Grid# | G4 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
