Mycobacterium phage Trouble
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Detailed Information for Phage Trouble
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTrevor Sughrue
Year Found2008
Location FoundSanta Cruz, CA USA
Finding InstitutionUniversity of California, Santa Cruz
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates36.998917 N, 122.052767 W Map
Discovery NotesFrom Merryl Compost Heap, depth of ~2 inches, 60°F
From rich soil in a compost heap in Merrill College at UC Santa Cruz
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 17, 2012
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage45
Genome length (bp)52102
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGATGGTAA
GC Content63.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA1
Cluster Life CycleTemperate
Other Cluster Members

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Afua (None)

Ajaxious (None)

Anonia (None)

BaxterTD (None)

Blazer (None)

BlueWhiteSuper (None)

BrownWilse (None)

BuddyNugget (None)

BuzzKiehl (None)

Chara (None)

Clarabelle (None)

Cornelius42 (None)

Cujo (None)

Dannah (None)

Drogon (None)

EGMO (None)

Fido (None)

Fieldrain (None)

FireFlyEcho (None)

FrannyJoe (None)

Freedman (None)

Geezy (None)

Gracie1 (None)

Hata (None)

HerbSipe (None)

HotBoxer (None)

ILoveMyInterns (None)

Interokirkus (None)

JeanneMarie (None)

JeckyllnHyde (None)

Julio (None)

Kilomegawatt (None)

Krzemiun (None)

LaChupacabra (None)

Lakemo2013 (None)

LilaGirl (None)

LilMac (None)

Lintoc (None)

LittleDude (None)

McKinnon95 (None)

MelBell (None)

MelJoe (None)

MortyC137 (None)

Nakoi (None)

Noodle17 (None)

Norris007 (None)

Ousia (None)

Pbnep65 (None)

PDonaz (None)

PerryPatch (None)

Phern (None)

Poofy (None)

Pwombat (None)

Raziel (None)

RedJewel (None)

Roetering (None)

Rover (None)

Scooter (None)

Serena (None)

Shayla (None)

Skpmule (None)

SmallSoldier (None)

SnapDragon (None)

SoletiStotinki (None)

SoupAstar (None)

Star (None)

Tala (None)

Terrance (None)

Terrence (None)

Tiff5 (None)

TizzleFlarp (None)

TosSolNia (None)

TreeHugger (None)

UMulchMonster (None)

Unsequencer (None)

Yost (None)

ZheroDRT (None)

Abrogate (A1)

Acme (A1)

Adahisdi (A1)

Aeneas (A1)

AFIS (A1)

Alberto11 (A1)

Alsfro (A1)

Alvin (A1)

Anglerfish (A1)

Arcanine (A1)

Arlo (A1)

Atkinbua (A1)

Barriga (A1)

Beatrix (A1)

BeesKnees (A1)

Bethlehem (A1)

Big3 (A1)

Bigfoot (A1)

BigMamaLlama (A1)

BigMau (A1)

BigPaolini (A1)

BillKnuckles (A1)

Bircsak (A1)

Bison (A1)

Blue (A1)

Bob3 (A1)

Bones (A1)

BPBiebs31 (A1)

Briton15 (A1)

Brody (A1)

Bruns (A1)

Brutter19 (A1)

Bullseye1 (A1)

Bullseyed (A1)

Buttons (A1)

Bxb1 (A1)

CactusRose (A1)

Cagedmage (A1)

Cedar (A1)

Charlotte (A1)

ConceptII (A1)

Conlectus (A1)

Corky (A1)

Corvo (A1)

Crispicous1 (A1)

Cueylyss (A1)

DD5 (A1)

DoodleBob (A1)

Doom (A1)

Dreamboat (A1)

DreamCatcher (A1)

DrFeelGood (A1)

Dulcie (A1)

Dynamix (A1)

EasyLeonard (A1)

Edtherson (A1)

Euphoria (A1)

Fajezeel (A1)

Fascinus (A1)

Fenn (A1)

Ferocissimum (A1)

FlowerChild (A1)

Forsytheast (A1)

FourStarNutty (A1)

Francis47 (A1)

Froghopper (A1)

Fushigi (A1)

GageAP (A1)

Gompeii16 (A1)

Graduation (A1)

Greg (A1)

GummyWorm (A1)

HanShotFirst (A1)

HansPay (A1)

HermioneGrange (A1)

Homines (A1)

Hope4ever (A1)

Ichabod (A1)

ILeeKay (A1)

JackSparrow (A1)

Jasper (A1)

JC27 (A1)

JCfoboat (A1)

Kanely (A1)

KatAttack (A1)

KBG (A1)

Kelcifer (A1)

Killigrew (A1)

KingLeonidas (A1)

KSSJEB (A1)

Kugel (A1)

Kykar (A1)

KyMonks1A (A1)

Lamina13 (A1)

Lesedi (A1)

Littlefoot32 (A1)

Lockley (A1)

Long2B (A1)

Lopton (A1)

Magnar (A1)

Magnito (A1)

Makemake (A1)

Manatee (A1)

Marcell (A1)

Maroc7 (A1)

McGuire (A1)

MetalQZJ (A1)

Michley (A1)

Mimicat (A1)

Minion (A1)

Moose (A1)

Moter17 (A1)

MPlant7149 (A1)

MrGordo (A1)

Mryolo (A1)

Mule (A1)

Museum (A1)

Naira (A1)

NEHalo (A1)

Nepal (A1)

Nerujay (A1)

Nhonho (A1)

Niza (A1)

Oogway (A1)

Osoo (A1)

Oxente (A1)

PacerPaul (A1)

Papez (A1)

Pari (A1)

Parks1 (A1)

PattyP (A1)

Pepe (A1)

Perseus (A1)

Petp2012 (A1)

Petruchio (A1)

PherrisBueller (A1)

PhrostyMug (A1)

Pinto (A1)

Pippin (A1)

Pita2 (A1)

PSullivan (A1)

Raindrum (A1)

Rhynn (A1)

RidgeCB (A1)

Rigel (A1)

Ringer (A1)

Riporf (A1)

Rocky (A1)

Rohr (A1)

Rufus (A1)

Ruotula (A1)

Rutherferd (A1)

Sagefire (A1)

SarFire (A1)

SargentShorty9 (A1)

Scowl (A1)

Seabiscuit (A1)

SF1Gamma (A1)

SF5Eta (A1)

Sibs6 (A1)

SkiPole (A1)

Smairt (A1)

Smeagol (A1)

Snazzy (A1)

Solon (A1)

SpikeBT (A1)

Spite (A1)

SunTzu (A1)

SwissCheese (A1)

Switzer (A1)

Target (A1)

Tasp14 (A1)

TechBARfly (A1)

TheloniousMonk (A1)

Thor (A1)

Tommy (A1)

Traft412 (A1)

Treddle (A1)

Tripl3t (A1)

Trouble (A1)

Turj99 (A1)

U2 (A1)

Uffda (A1)

Venus (A1)

Violet (A1)

Watermelon (A1)

Wheeler (A1)

Widget (A1)

Wilkins (A1)

Zeeba (A1)

Zeeculate (A1)

Zelezo (A1)

Zephyr (A1)

Zeuska (A1)

Blitzkrieg (A10)

Chupacabra (A10)

ElSilvo (A10)

Goose (A10)

KittenMittens (A10)

Kristoff (A10)

Lindberg (A10)

OKCentral2016 (A10)

Rebeuca (A10)

RhynO (A10)

Severus (A10)

Trike (A10)

Twister (A10)

WalterMcMickey (A10)

Bachome (A11)

Bowtie (A11)

Bud (A11)

Ebony (A11)

Et2Brutus (A11)

Fibonacci (A11)

Flaverint (A11)

Hutc2 (A11)

IshmaelIII (A11)

Jabith (A11)

Joselito (A11)

Lucivia (A11)

Mabel (A11)

Mulciber (A11)

Munch (A11)

Orange (A11)

Salz (A11)

Snape (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Steamy (A12)

Phlei (A13)

Jeeves (A14)

Luchador (A14)

Onism (A14)

Anon (A15)

JSwag (A15)

KatherineG (A15)

LastResort (A15)

Nedarya (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Waits (A15)

EagleEye (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

AbbyPaige (A2)

Acolyte (A2)

Adzzy (A2)

Agape74 (A2)

AnnaL29 (A2)

Anselm (A2)

Aquarose (A2)

ArcherNM (A2)

AsepTech (A2)

Asuzu (A2)

Bactobuster (A2)

Baehexic (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Brooklyn (A2)

Buddy (A2)

Bugsy (A2)

Bulldog (A2)

Centaur (A2)

Changeling (A2)

Charity (A2)

Che12 (A2)

Cheriba (A2)

ChipMunk (A2)

ClumpyY (A2)

CRB1 (A2)

Crucio (A2)

Crush (A2)

D29 (A2)

Dalmatian (A2)

DBQu4n (A2)

Death (A2)

Drake55 (A2)

DudeLittle (A2)

Echild (A2)

ElFluff (A2)

Epoch (A2)

Equemioh13 (A2)

EvilGenius (A2)

Fameo (A2)

FiringLine (A2)

First (A2)

Flare16 (A2)

GaugeLDP (A2)

Georgie2 (A2)

Goethe (A2)

GoZags (A2)

GridersLegacy (A2)

Heffalump (A2)

IronMan (A2)

Iwokeuplikedis (A2)

Jaan (A2)

Jerm (A2)

Jinga3000 (A2)

JoshKayV (A2)

Journey13 (A2)

Jsquared (A2)

Kakar (A2)

Kalpine (A2)

Kempella (A2)

Kerberos (A2)

KingSt (A2)

Kupalo (A2)

L5 (A2)

Laderlappen (A2)

LadyBird (A2)

Larenn (A2)

Leogania (A2)

LilTurb (A2)

Lincaica11 (A2)

Lokk (A2)

Loser (A2)

Maduna (A2)

Malec (A2)

Masha1295 (A2)

Miko (A2)

MissWhite (A2)

MrBarnaby (A2)

Naji (A2)

NaSiaTalie (A2)

Nemo (A2)

NicoleTera (A2)

Norris (A2)

NothingSpecial (A2)

Odin (A2)

Ph8s (A2)

Phaded (A2)

Pim618 (A2)

Piro94 (A2)

Pomar16 (A2)

Power (A2)

Pukovnik (A2)

QueenB2 (A2)

QueenBeesly (A2)

Rachaly (A2)

Radar (A2)

Rauana (A2)

RedRock (A2)

Retro23 (A2)

Scorpio7 (A2)

SemperFi (A2)

Serenity (A2)

Shipshape (A2)

SnapTap (A2)

StarStuff (A2)

Stumpy (A2)

Superchunk (A2)

SweetiePie (A2)

Tiberius20 (A2)

TipsytheTRex (A2)

Tomathan (A2)

Trixie (A2)

Turbido (A2)

Tyrone (A2)

Updawg (A2)

Whabigail7 (A2)

WideWale (A2)

Woozle13 (A2)

Yahighness (A2)

Zaaboot (A2)

Anthony (A20)

ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

Anubis (A3)

Assassinator (A3)

AugsMagnumOpus (A3)

BabyRay (A3)

Beauxregard13 (A3)

BreSam8 (A3)

BuzzBuzz (A3)

Bxz2 (A3)

DaHudson (A3)

Daishi (A3)

Dieselweasel (A3)

Dumpling (A3)

EpicPhail (A3)

Farber (A3)

Fernando (A3)

FloMo (A3)

Fred313 (A3)

Gemma (A3)

GingkoMaracino (A3)

Gravata (A3)

Groupthink (A3)

Grum1 (A3)

GtownJaz (A3)

HelDan (A3)

Heliosoles (A3)

Hercules11 (A3)

Hookmount (A3)

Idleandcovert (A3)

Isca (A3)

JenCasNa (A3)

JeppNRM (A3)

JHC117 (A3)

Jobu08 (A3)

KADY (A3)

Kalb97 (A3)

Kalnoky (A3)

LadyPhaGa (A3)

Lambert1 (A3)

Lilith (A3)

Louie6 (A3)

LugYA (A3)

MadMarie (A3)

Mainiac (A3)

Malinsilva (A3)

Margo (A3)

Marie (A3)

Marius (A3)

MarQuardt (A3)

MatrixWest360 (A3)

Methuselah (A3)

Microwolf (A3)

Misomonster (A3)

MoneyMay (A3)

MuchMore (A3)

Noella (A3)

Norbert (A3)

OlanP (A3)

Ollie (A3)

Ostojic (A3)

P28Green (A3)

Panamaxus (A3)

Penny1 (A3)

PGHhamlin (A3)

Phantastic (A3)

PhishRPhriends (A3)

Phoxy (A3)

Phranny (A3)

Pistachio (A3)

Pocahontas (A3)

Popcicle (A3)

PotatoSplit (A3)

Puppy (A3)

PurpleHaze (A3)

QuinnKiro (A3)

Reba (A3)

Roadbarry13 (A3)

Rockstar (A3)

Romeo (A3)

Sabia (A3)

Sabinator (A3)

SoilDragon (A3)

Soshari (A3)

Spike509 (A3)

Stagni (A3)

Stephen (A3)

StepMih (A3)

Taurus (A3)

Texage (A3)

Tiffany (A3)

TNguyen7 (A3)

Todacoro (A3)

Veracruz (A3)

Vix (A3)

Watson (A3)

Wonder (A3)

Wooldri (A3)

Zetzy (A3)

AbbysRanger (A4)

Abdiel (A4)

Achebe (A4)

Albee (A4)

Annyong (A4)

Arturo (A4)

AvatarAhPeg (A4)

Avle17 (A4)

Baby16 (A4)

Backyardigan (A4)

Bacon (A4)

Badger (A4)

Bartimeaus (A4)

BellusTerra (A4)

Blackmoor (A4)

Bombshell (A4)

Broseidon (A4)

Bruiser (A4)

BubbleTrouble (A4)

Bumblebee11 (A4)

Burger (A4)

Caelakin (A4)

Camperdownii (A4)

CentreCat (A4)

Cerulean (A4)

ChampagnePapi (A4)

Cici (A4)

Cindaradix (A4)

Citius (A4)

Clarenza (A4)

Cocoaberry (A4)

Commander (A4)

Connomayer (A4)

DAgoa1 (A4)

Datway (A4)

DCNugh (A4)

Deano (A4)

Decimator (A4)

Dhanush (A4)

DirtyDunning (A4)

Druantia (A4)

Eagle (A4)

Eapen (A4)

Eris (A4)

Eros (A4)

Flapjack (A4)

Florean (A4)

Flux (A4)

Funston (A4)

FuZGrapes (A4)

Gadost (A4)

Gaius (A4)

HamSlice (A4)

Heisenburg (A4)

Holli (A4)

Houdini22 (A4)

ICleared (A4)

Iracema64 (A4)

Jaykayelowell (A4)

JetBlade (A4)

JoongJeon (A4)

Kampy (A4)

Katalie136 (A4)

KFPoly (A4)

Kingmustik0402 (A4)

Kratark (A4)

Kyee (A4)

Lemur (A4)

LeoAvram (A4)

LHTSCC (A4)

LittleB (A4)

LittleGuy (A4)

Lorenzo (A4)

LucyGoose (A4)

Massacre (A4)

Maverick (A4)

Mazhar510 (A4)

Medusa (A4)

MeeZee (A4)

Melvin (A4)

Millski (A4)

Milton (A4)

Miramae (A4)

Morpher26 (A4)

Morrow (A4)

Mundrea (A4)

Nemo27 (A4)

NorthStar (A4)

NotAPhaseMom (A4)

Nyxis (A4)

Obama12 (A4)

Ohfah (A4)

Palestino (A4)

Peaches (A4)

PeterPeter (A4)

Phacado (A4)

Phighter1804 (A4)

Pipcraft (A4)

Relief (A4)

Romney (A4)

Roosevelt (A4)

Ruin (A4)

Sabertooth (A4)

Shaka (A4)

SheldonCooper (A4)

Skipitt (A4)

SophiesChoice (A4)

Stasia (A4)

Thanksgivukkah (A4)

TimCurry (A4)

TinaFeyge (A4)

Tinybot (A4)

TiroTheta9 (A4)

Tpap (A4)

TygerBlood (A4)

Wander (A4)

Wilbur (A4)

Wile (A4)

Wizard007 (A4)

Xena (A4)

AgentM (A5)

Airmid (A5)

Aragog (A5)

Archetta (A5)

Barbarella (A5)

Benedict (A5)

Bluefalcon (A5)

Chadwick (A5)

Conspiracy (A5)

Coog (A5)

Cuco (A5)

Dublin (A5)

Electri (A5)

ElTiger69 (A5)

ForGetIt (A5)

George (A5)

HuhtaEnerson15 (A5)

Jabiru (A5)

Jovo (A5)

Lev2 (A5)

LittleCherry (A5)

MarysWell (A5)

Midas2 (A5)

Naca (A5)

Phlorence (A5)

Scorpia (A5)

Swirley (A5)

Tarynearal (A5)

Theia (A5)

Tiger (A5)

UnionJack (A5)

WootieJr (A5)

Yolo (A5)

Artemis2UCLA (A6)

Blue7 (A6)

CloudWang3 (A6)

DaVinci (A6)

EricB (A6)

Gladiator (A6)

GreedyLawyer (A6)

Gruunaga (A6)

Hammer (A6)

Hexamo (A6)

Isiphiwo (A6)

Jeffabunny (A6)

JewelBug (A6)

Jordennis (A6)

Kazan (A6)

Koko (A6)

McFly (A6)

Paper (A6)

Pmask (A6)

Priamo (A6)

SuperAwesome (A6)

ToneTone (A6)

VohminGhazi (A6)

Wiks (A6)

WunderPhul (A6)

Yokurt (A6)

Zaka (A6)

Zulu (A6)

HINdeR (A7)

Sheen (A7)

Timshel (A7)

Astro (A8)

Cyrano (A8)

Dixon (A8)

Expelliarmus (A8)

Fredward (A8)

Kirpan (A8)

Machaera (A8)

Mitchell (A8)

NearlyHeadless (A8)

Roary (A8)

Saintus (A8)

Shresh (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

BogosyJay (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

Dolasoaandri (A9)

DreamTeam1 (A9)

Eidsmoe (A9)

Elephantoon (A9)

ExplosioNervosa (A9)

HortumSL17 (A9)

Klaus (A9)

LoneWolf (A9)

Maminiaina (A9)

Myxus (A9)

Noelle (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Priya (A9)

Qobbit (A9)

Rahalelujah (A9)

Scherzo (A9)

ShadowHunter (A9)

SheaKeira (A9)

Toaka (A9)

Tubs (A9)

Ugenie5 (A9)

Yecey3 (A9)

Annotation StatusIn GenBank
Plaque NotesClear and fast growing. Plaque diameter 3 mm.
MorphotypeSiphoviridae
Number of Genes94
Has been Phamerated?Yes
Gene List

Click to View

Trouble_1
(HNH domain protein)

Trouble_2

Trouble_3

Trouble_4
(HNH domain protein)

Trouble_5
(tail protein)

Trouble_6

Trouble_7

Trouble_8

Trouble_9
(LysA)

Trouble_10
(LysB)

Trouble_11
(terminase large subunit)

Trouble_12
(portal protein)

Trouble_13
(capsid maturation protease)

Trouble_14
(scaffold protein)

Trouble_15
(major capsid protein)

Trouble_16

Trouble_17

Trouble_18

Trouble_19

Trouble_20

Trouble_21
(major tail subunit)

Trouble_22
(tail assembly chaperone)

Trouble_23
(tail assembly chaperone)

Trouble_24
(tape measure protein)

Trouble_25
(HNH domain protein)

Trouble_26
(minor tail subunit)

Trouble_27

Trouble_28
(minor tail subunit)

Trouble_29

Trouble_30

Trouble_31

Trouble_32
(minor tail subunit)

Trouble_33
(D-ala-D-ala carboxypeptidase)

Trouble_34

Trouble_35

Trouble_36

Trouble_37

Trouble_38
(serine integrase)

Trouble_39

Trouble_40

Trouble_41

Trouble_42

Trouble_43

Trouble_44

Trouble_45
(HNH domain protein)

Trouble_46
(DNA polymerase A)

Trouble_47

Trouble_48

Trouble_49

Trouble_50

Trouble_51

Trouble_52
(RNA polymerase sigma factor)

Trouble_53

Trouble_54
(RDF protein)

Trouble_55

Trouble_56

Trouble_57
(DNA primase)

Trouble_58
(DNA primase)

Trouble_59

Trouble_60

Trouble_61
(Endo VII protein)

Trouble_62

Trouble_63
(NrdH)

Trouble_64
(DnaB-like helicase)

Trouble_65

Trouble_66

Trouble_67
(DNA binding domain protein)

Trouble_68
(DNA methyltransferase)

Trouble_69

Trouble_70

Trouble_71

Trouble_72

Trouble_73

Trouble_74

Trouble_75
(immunity repressor)

Trouble_76

Trouble_77

Trouble_78

Trouble_79

Trouble_80

Trouble_81

Trouble_82

Trouble_83

Trouble_84

Trouble_85

Trouble_86

Trouble_87

Trouble_88

Trouble_89

Trouble_90

Trouble_91

Trouble_92

Trouble_93

Trouble_94

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKF024724
Refseq NumberNC_022062
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2008 UCSC Trouble TS
SEA Lysate Titer 8 x 10^9 pfu/mL
Date of SEA Lysate Titering Oct 29, 2008
Pitt Freezer Box# 3
Pitt Freezer Box Grid# C1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload