Mycobacterium phage VasuNzinga
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Detailed Information for Phage VasuNzinga
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByVasupradha Girish, Nzinga Walker
Year Found2014
Location FoundWest Lafayette, IN US
Finding InstitutionPurdue University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates40.420934 N, 86.92076 W Map
Discovery NotesSample collected on 09/01/14, at 1:10 pm. The depth of the soil was around 3.5-4 cm. The approximate moisture of the soil was 70%. Was on the side of a path, covered with leaves. HTL titre: 7.5*10^7 pfu/ml.
Naming NotesNamed after the two people who isolated the phage: Vasupradha Girish and Nzinga Walker.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 6, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1246
Genome length (bp)64911
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceGCGCGCAGCGC
GC Content63.4%
Sequencing NotesThis phage was identified as being uncommon during a large DOGEMS screening run, then subsequently grown from frozen stock and sequenced.
Fasta file available?Yes: Download fasta file
Characterization
ClusterS
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe plaque was around 2mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

VasuNzinga_1

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VasuNzinga_14

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(HNH endonuclease)

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(DNA methylase)

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(terminase)

VasuNzinga_36
(portal protein)

VasuNzinga_37
(MuF-like minor capsid protein)

VasuNzinga_38
(capsid maturation protease)

VasuNzinga_39
(Capsid decoration protein)

VasuNzinga_40
(Major capsid protein)

VasuNzinga_41
(Head-to-tail connector complex protein)

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(head-to-tail connector complex protein)

VasuNzinga_45
(major tail protein)

VasuNzinga_46
(Tail assembly chaperone)

VasuNzinga_47
(Tail assembly chaperone)

VasuNzinga_48

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(minor tail protein)

VasuNzinga_50
(minor tail protein)

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(HNH endonuclease)

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(Lysin A)

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(Lysin B)

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(holin)

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VasuNzinga_64
(HTH DNA binding domain)

VasuNzinga_65
(exonuclease)

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(HNH endonuclease)

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(DprA-like DNA processing chain)

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(O-methyl transferase)

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(glycosyl transferase)

VasuNzinga_84
(Galactosyltransferase)

VasuNzinga_85

VasuNzinga_86
(glycosyltransferase)

VasuNzinga_87

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VasuNzinga_89

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(HNH endonuclease)

VasuNzinga_91
(Minor tail protein)

VasuNzinga_92
(minor tail protein)

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VasuNzinga_107

VasuNzinga_108

VasuNzinga_109

VasuNzinga_110

VasuNzinga_111
(glutamine amidotransferase)

VasuNzinga_112

VasuNzinga_113

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH727562
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 12
Pitt Freezer Box Grid# A12
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload