Mycobacterium phage Wildcat
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Detailed Information for Phage Wildcat
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJ. Falbo
Year Found2003
Location FoundLatrobe , PA USA
Finding InstitutionLatrobe High School
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates40.28113 N, 79.376446 W Map
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 8, 2006
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodSanger Sequencing
Genome length (bp)78296
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
GC Content57.2%
Sequencing NotesJuly 28, 2014

Wildcat's 3' overhang is included at the left end of the genome.

This is because in the early days of mycobacteriophage sequencing, 3' overhangs were often reported at the left end of genomes. This is true for L5, D29, TM4, and others. At some point, we decided to put 3' overhangs at the right end because then you're representing one of the two real strands of DNA completely. But some phages got grandfathered in with left-end 3' overhangs, since their genomes were already submitted to GenBank by the time we made the switch.

That explains why Wildcat's overhang is at the left end. It doesn't explain why only 10/11ths of the overhang is at the left end. That seems to have been an error in creating the final fasta file.
Fasta file available?Yes: Download fasta file
Characterization
ClusterV
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes148
Number of tRNAs24
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

Click to View

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Publication Info
Uploaded to GenBank?Yes
GenBank AccessionDQ398052
Refseq NumberNC_008206
Archiving Info
Archiving status Archived
Pitt Freezer Box# 8
Pitt Freezer Box Grid# A10
Available Files
Plaque PictureDownload
Virtual Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload