Mycobacterium phage Xeno
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Detailed Information for Phage Xeno
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAmy Karlberg
Year Found2014
Location FoundNew Haven, CT USA
Finding InstitutionSouthern Connecticut State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature42°C
GPS Coordinates41.333611 N, 72.9525 W Map
Discovery NotesThe area that I collected my phage from is on a foot path and was about one foot away from a murky puddle in the dirt. It was taken from a soil sample with a green fuzzy substance on top. The depth of the sample was 1.5 cm and the temperature outside was 69 degrees F and sunny.
It has an average capsid head diameter of 61.5438 nm and a average tail length of 178.2136 nm. Xeno's capsid head standard deviation is 1.50897 nm and the tail length standard deviation is 8.37814 nm.
Naming NotesXeno is named after Xenophilius Lovegood from Harry Potter. I love Harry Potter, so I wanted to relate my phage to it in some way. Since Xenophilius Lovegood is a bit of an odd character, I thought it was fitting to name my mycobacteriophage after him.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 6, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage493
Genome length (bp)42395
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceCCCGCCGCCTTGG
GC Content66.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterN
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesXeno is lytic and has a diameter of 2 mm.
MorphotypeSiphoviridae
Number of Genes69
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKU935728
Refseq NumberNC_031243
Archiving Info
Archiving status Archived
SEA Designator 2014SCSUxenoAK
SEA Lysate Titer 4.6x 10^10
Date of SEA Lysate Titering Oct 13, 2014
Pitt Freezer Box# 10
Pitt Freezer Box Grid# C3
Available Files
EM PictureDownload
Final DNAMaster FileDownload