Mycobacterium phage Zakhe101
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Detailed Information for Phage Zakhe101
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByZakhe M Mcunu
Year Found2014
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates29.817371 S, 30.942245 E Map
Discovery NotesThis phage was found near the laboratoy soil, the soil that it was extracted from was moist and dark. The colonies were so small and grow after a 24hr incubation at 37C. It is location 3A on the lysate prepared
Naming NotesThe name of this phage comes directly to my own name Zakhe meaning 'make'. This phage is morphologically different compare to other phages that were isolated as it had a prolate head and tail. the code 101 means that it usually have a spot that include plaques inside a rare feacture in the immunity test. Therefore its name is Zakhe101
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 29, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage215
Genome length (bp)69653
Character of genome ends3' Sticky Overhang
Overhang Length4 bases
Overhang SequenceGTGT
GC Content65.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterO
Subcluster--
Other Cluster Members
Plaque NotesThe plaques were a circular shape. the head of the phage is prolate similar to corndog and Dylan phages. From the digest picture it can be seen against Da Vinci on position 2 that it forms a clear spot.
MorphotypeSiphoviridae
Final annotation complete?No
Number of Genes123
Has been Phamerated?Yes
Gene List

Click to View

Zakhe101_1

Zakhe101_2

Zakhe101_3

Zakhe101_4

Zakhe101_5

Zakhe101_6
(DNA Methylase)

Zakhe101_7
(DNA Methylase)

Zakhe101_8

Zakhe101_9

Zakhe101_10

Zakhe101_11
(Endonuclease VII)

Zakhe101_12

Zakhe101_13

Zakhe101_14

Zakhe101_15

Zakhe101_16

Zakhe101_17

Zakhe101_18

Zakhe101_19

Zakhe101_20

Zakhe101_21
(DNA primase/polymerase)

Zakhe101_22

Zakhe101_23

Zakhe101_24
(WhiB transcriptional regulator)

Zakhe101_25

Zakhe101_26

Zakhe101_27

Zakhe101_28
(HNH Endonuclease)

Zakhe101_29

Zakhe101_30

Zakhe101_31
(Terminase large subunit)

Zakhe101_32
(Portal protein)

Zakhe101_33
(O-methyltransferase)

Zakhe101_34

Zakhe101_35
(Glycosyltransferase)

Zakhe101_36
(Glycosyltransferase)

Zakhe101_37

Zakhe101_38

Zakhe101_39
(Major capsid protein)

Zakhe101_40

Zakhe101_41

Zakhe101_42

Zakhe101_43

Zakhe101_44

Zakhe101_45

Zakhe101_46

Zakhe101_47

Zakhe101_48

Zakhe101_49

Zakhe101_50

Zakhe101_51

Zakhe101_52
(Tail assembly chaperone)

Zakhe101_53
(Tail Assembly Chaperone)

Zakhe101_54
(Tape measure protein)

Zakhe101_55
(minor tail protein)

Zakhe101_56
(minor tail subunit)

Zakhe101_57
(minor tail subunit)

Zakhe101_58
(minor tail subunit)

Zakhe101_59
(D-ala-D-ala- carboxypeptidase)

Zakhe101_60
(minor tail subunit)

Zakhe101_61
(minor tail subunit)

Zakhe101_62

Zakhe101_63

Zakhe101_64

Zakhe101_65
(LysA)

Zakhe101_66
(LysB)

Zakhe101_67
(holin)

Zakhe101_68

Zakhe101_69

Zakhe101_70

Zakhe101_71

Zakhe101_72
(HTH DNA binding protein)

Zakhe101_73

Zakhe101_74

Zakhe101_75

Zakhe101_76

Zakhe101_77

Zakhe101_78

Zakhe101_79

Zakhe101_80

Zakhe101_81

Zakhe101_82
(DNA Pol III)

Zakhe101_83

Zakhe101_84

Zakhe101_85

Zakhe101_86
(Ku protein)

Zakhe101_87

Zakhe101_88

Zakhe101_89
(ParB-like protein)

Zakhe101_90

Zakhe101_91

Zakhe101_92

Zakhe101_93

Zakhe101_94

Zakhe101_95

Zakhe101_96

Zakhe101_97

Zakhe101_98

Zakhe101_99

Zakhe101_100

Zakhe101_101

Zakhe101_102

Zakhe101_103

Zakhe101_104

Zakhe101_105

Zakhe101_106

Zakhe101_107

Zakhe101_108

Zakhe101_109

Zakhe101_110

Zakhe101_111

Zakhe101_112

Zakhe101_113

Zakhe101_114

Zakhe101_115

Zakhe101_116

Zakhe101_117

Zakhe101_118

Zakhe101_119

Zakhe101_120

Zakhe101_121

Zakhe101_122

Zakhe101_123

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT281796
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload