Mycobacterium phage ZygoTaiga
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Detailed Information for Phage ZygoTaiga
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCarmen Aguirre
Year Found2012
Location FoundDuluth, MN United States of America
Finding InstitutionCollege of St. Scholastica
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates46.8 N, 92.08 W Map
Discovery NotesThe phage we isolated was from a park in down town duluth. The location was surrounded by streets on all sides and had a playground within 300 feet. It was a grassy, and damp area near trees. I isolated the phage around 12:00am; the night was cloudy, foggy and colder (around 40*F) which made the soil sample very damp.
Naming NotesA blend of the Taiga forest near where I found my phage and zygote cause our phage was one of a kind.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 12, 2013
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage110
Sanger Finishing Reactions2
Genome length (bp)157204
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Other Cluster Members

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Boonesok (None)

CLach (None)

DavidMina (None)

DitchDweller (None)

Elpis (None)

Genet (None)

Hard2get (None)

Hippocrates (None)

Kali (None)

LittleSpot (None)

Lsquared (None)

Marlex (None)

Minipoke (None)

PeeWee (None)

Pesky7 (None)

Phomnie (None)

Phyllis (None)

Roku (None)

Sagamore (None)

Samarai (None)

TinyC (None)

Trilla23 (None)

Turret (None)

Squidney (A3)

Ading (C1)

Adlitam (C1)

Alice (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Atlas (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Breeniome (C1)

Bxz1 (C1)

Cali (C1)

Catera (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Delilah (C1)

Derek (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Exxus285 (C1)

Flabslab (C1)

FoxtrotP1 (C1)

FrayBell (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

HarrySpotter (C1)

HyRo (C1)

InterFolia (C1)

Iota (C1)

Isee1101 (C1)

JennanaJr (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LRRHood (C1)

Lukilu (C1)

Lysocool (C1)

Melpomini (C1)

MePac (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Napoleon13 (C1)

Nappy (C1)

Nicolasphaqe (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patch (C1)

Patter (C1)

Phox (C1)

Pier (C1)

Pio (C1)

Pleione (C1)

QBert (C1)

Rizal (C1)

SandySandal (C1)

ScottMcG (C1)

Sebastian (C1)

Sebata (C1)

ShankPowerGod (C1)

Shaqnato (C1)

ShiaLabeouf (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

Stubby (C1)

Teardrop (C1)

TheO (C1)

Thuathua (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Tyke (C1)

Wally (C1)

Willis (C1)

Yucca (C1)

Zeenon (C1)

ZygoTaiga (C1)

Matcha (C2)

Myrna (C2)

Phabba (C2)

SimSimmers (C2)

SrishMeg2525 (C2)

Plaque NotesOur phage that we isolated are very small, clear plaques and this shows to be very consistent throughout our purification and empirical testing. From my observations in the past 2 months I have come to a conclusion that my plaque seems to be a lytic phage (of course there is no certainty in that but it has shown no expression of temperate characteristics). Our phage is very slow growing and can be somewhat difficult to work with, but our plates have come out very consistent.
MorphotypeMyoviridae
Final annotation complete?Yes
Number of Genes232
Number of tRNAs32
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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ZygoTaiga_1

ZygoTaiga_2

ZygoTaiga_3
(nucleotidyltransferase)

ZygoTaiga_4

ZygoTaiga_5

ZygoTaiga_6

ZygoTaiga_7

ZygoTaiga_8

ZygoTaiga_9

ZygoTaiga_10

ZygoTaiga_11

ZygoTaiga_12

ZygoTaiga_13

ZygoTaiga_14

ZygoTaiga_15

ZygoTaiga_16

ZygoTaiga_17

ZygoTaiga_18

ZygoTaiga_19
(Histidine Triad domain)

ZygoTaiga_20

ZygoTaiga_21

ZygoTaiga_22

ZygoTaiga_23

ZygoTaiga_24

ZygoTaiga_25

ZygoTaiga_26

ZygoTaiga_27

ZygoTaiga_28

ZygoTaiga_29
(Band 7)

ZygoTaiga_30

ZygoTaiga_31

ZygoTaiga_32

ZygoTaiga_33

ZygoTaiga_34

ZygoTaiga_35

ZygoTaiga_36

ZygoTaiga_37

ZygoTaiga_38

ZygoTaiga_39

ZygoTaiga_40

ZygoTaiga_41

ZygoTaiga_42

ZygoTaiga_43

ZygoTaiga_44

ZygoTaiga_45

ZygoTaiga_46

ZygoTaiga_47

ZygoTaiga_48

ZygoTaiga_49

ZygoTaiga_50

ZygoTaiga_51

ZygoTaiga_52

ZygoTaiga_53

ZygoTaiga_54

ZygoTaiga_55

ZygoTaiga_56

ZygoTaiga_57

ZygoTaiga_58

ZygoTaiga_59

ZygoTaiga_60

ZygoTaiga_61

ZygoTaiga_62

ZygoTaiga_63
(HTH DNA binding domain)

ZygoTaiga_64

ZygoTaiga_65

ZygoTaiga_66

ZygoTaiga_67

ZygoTaiga_68

ZygoTaiga_69

ZygoTaiga_70

ZygoTaiga_71

ZygoTaiga_72

ZygoTaiga_73

ZygoTaiga_74

ZygoTaiga_75

ZygoTaiga_76

ZygoTaiga_77

ZygoTaiga_78

ZygoTaiga_79

ZygoTaiga_80

ZygoTaiga_81

ZygoTaiga_82
(ParB-like)

ZygoTaiga_87

ZygoTaiga_88

ZygoTaiga_89
(Portal)

ZygoTaiga_90

ZygoTaiga_91

ZygoTaiga_92
(ThyX-like)

ZygoTaiga_93

ZygoTaiga_94
(Phosphoesterase)

ZygoTaiga_95
(LysM)

ZygoTaiga_96

ZygoTaiga_97

ZygoTaiga_98
(Major capsid)

ZygoTaiga_99
(zinc-finger DNA binding domain)

ZygoTaiga_100

ZygoTaiga_101

ZygoTaiga_102

ZygoTaiga_103

ZygoTaiga_104

ZygoTaiga_105

ZygoTaiga_106

ZygoTaiga_107

ZygoTaiga_108

ZygoTaiga_109

ZygoTaiga_110

ZygoTaiga_111

ZygoTaiga_112

ZygoTaiga_113

ZygoTaiga_114

ZygoTaiga_115

ZygoTaiga_116

ZygoTaiga_117

ZygoTaiga_118

ZygoTaiga_119
(N-acetyl transferase)

ZygoTaiga_120

ZygoTaiga_121

ZygoTaiga_122

ZygoTaiga_123

ZygoTaiga_124

ZygoTaiga_125

ZygoTaiga_126

ZygoTaiga_127

ZygoTaiga_128
(Tail assembly chaperone)

ZygoTaiga_129
(Tail assembly chaperone)

ZygoTaiga_130
(Tapemeasure)

ZygoTaiga_131

ZygoTaiga_132

ZygoTaiga_133

ZygoTaiga_134
(Minor tail protein)

ZygoTaiga_135

ZygoTaiga_136
(Minor tail protein)

ZygoTaiga_137

ZygoTaiga_138
(Baseplate J)

ZygoTaiga_139
(HNH endonuclease domain)

ZygoTaiga_140

ZygoTaiga_141

ZygoTaiga_142

ZygoTaiga_143

ZygoTaiga_144

ZygoTaiga_145

ZygoTaiga_146

ZygoTaiga_147

ZygoTaiga_148

ZygoTaiga_149

ZygoTaiga_150

ZygoTaiga_153

ZygoTaiga_154
(Peptidyl tRNA hydrolase domain)

ZygoTaiga_169
(D-Ala D-Ala carboxypeptidase)

ZygoTaiga_170

ZygoTaiga_182

ZygoTaiga_183

ZygoTaiga_184
(Helicase)

ZygoTaiga_185

ZygoTaiga_186

ZygoTaiga_187

ZygoTaiga_188

ZygoTaiga_189

ZygoTaiga_190

ZygoTaiga_191

ZygoTaiga_192

ZygoTaiga_193

ZygoTaiga_194

ZygoTaiga_195
(ArgD)

ZygoTaiga_196

ZygoTaiga_197

ZygoTaiga_198

ZygoTaiga_199
(DnaC)

ZygoTaiga_200
(DnaB-like Helicase)

ZygoTaiga_201

ZygoTaiga_202
(DNA primase)

ZygoTaiga_203

ZygoTaiga_204
(DnaJ)

ZygoTaiga_205

ZygoTaiga_206
(DNA polymerase III alpha subunit)

ZygoTaiga_207
(IF1)

ZygoTaiga_208
(RecA)

ZygoTaiga_209

ZygoTaiga_210

ZygoTaiga_211

ZygoTaiga_212
(RusA)

ZygoTaiga_213

ZygoTaiga_214

ZygoTaiga_215

ZygoTaiga_216

ZygoTaiga_217

ZygoTaiga_218

ZygoTaiga_219

ZygoTaiga_222

ZygoTaiga_223

ZygoTaiga_224

ZygoTaiga_225

ZygoTaiga_226

ZygoTaiga_227

ZygoTaiga_228
(Ro)

ZygoTaiga_229

ZygoTaiga_230

ZygoTaiga_231

ZygoTaiga_232

ZygoTaiga_233

ZygoTaiga_234

ZygoTaiga_235

ZygoTaiga_236
(glycosyltransferase)

ZygoTaiga_237

ZygoTaiga_238

ZygoTaiga_239
(galactosyltransferase)

ZygoTaiga_240

ZygoTaiga_241
(glycosyltransferase)

ZygoTaiga_242
(putative casid decoration, structural)

ZygoTaiga_243

ZygoTaiga_244

ZygoTaiga_245

ZygoTaiga_246

ZygoTaiga_247
(LysA)

ZygoTaiga_248

ZygoTaiga_249
(LysB)

ZygoTaiga_250

ZygoTaiga_251

ZygoTaiga_252

ZygoTaiga_253

ZygoTaiga_254

ZygoTaiga_255
(Pnk)

ZygoTaiga_256

ZygoTaiga_257

ZygoTaiga_258
(Ser/Thr kinase)

ZygoTaiga_259

ZygoTaiga_260

ZygoTaiga_261
(PurA)

ZygoTaiga_262

ZygoTaiga_263

ZygoTaiga_264

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKM881426
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload