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Annotation: Protocols and Information
Annotation

Annotation

The genome editor used by phagehunters across the country is DNA Master. Our Annotation Guide included here gives very specific instruction on how we annotate our genomes. Included in annotation is gene prediction and functional assignments.
Protocol TitleDescription/Goal
A Collection of Annotated Maps
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Using a Local Database for Function Assignment
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To have easy access to current known protein function through use of a DNA Master created protein database.
References for Gene Functions
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DNA Master QuickStart Guide
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DNA Master QuickStart Guide
Reasons An Annotation May Be Returned During QC
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Several common things to look for to make sure you submit a high-quality annotation for QC.
Guiding Principles of Bacteriophage Genome Annotation
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Guiding Principles of Bacteriophage Genome Annotation
Function Assignment - Best Format
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Use this table to correctly transcribe functional assignments. This is not how to assign functions, but rather, once you know what function you want to assign, recording it in the best way possible.
Revised tRNA Protocol
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Available on-line tools have changed since the Annotation Guide was written. This protocol describes looking for relaxed tRNA structures using the tools that are now available.
Complete DNA Master Guide
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The complete Guide to DNA Master. Updated December 2014. Modest revisions for 2016-17. See "What's New" for further information. As the guide is improved, you will find updates available at this location.
What's New in the Annotation Guide for 2016-17 School Year
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To target the most prominent changes and/or updates to DNA Master and related materials for returning users. This document contains all previous What's New information.