Extraction: Protocols and Information
Extraction
The methods used in our lab for extracting clean DNA for sequencing, restriction digests, and further investigations are described here. It is important to know the concentration of phage (titer) before starting.| Protocol Title | Description/Goal |
|---|---|
| PCI/SDS DNA Extraction Details Download pdf |
To extract a high yield, clean DNA sample from 1 mL high titer phage lysate. |
| PCI DNA Extraction Details Download pdf |
To extract a high yield, clean DNA sample from 15 mL high titer phage lysate. |
| Small-Scale Phage Concentration Details Download pdf |
To concentrate a phage sample using a small-scale prep. |
| Phage DNA Extraction Using the Promega Clean-Up Kit Details Download pdf |
Extract phage genomic DNA using the Promega DNA Clean-Up Kit. |