Preparation: Protocols and Information
Preparation

Preparation

Protocols and instructions to get set up to begin phagehunting. Includes information on how/where to order lab supplies, how to make media, safety precautions, naming guidelines, etc.
Protocol TitleDescription/Goal
Preparing Working Media
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Finish preparing several common reagents (Phage Buffer, 7H9, MBTA) by adding final ingredients.
Preparing ADC
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Prepare ADC for use as the energy source for mycobacterial growth in 7H9 cultures or 7H10 plates.
Preparing Phage Buffer
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Prepare standard buffer for storing and diluting mycobacteriophages.
Preparing 7H9
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Prepare the base broth for use in growing mycobacterial cultures and plating mycobacterial lawns.
Ordering Information for Common Supplies
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This protocol contains the vendors from which we order the most common supplies, as well as the catalog numbers of those items.
M. smegmatis Media Additions
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A reference table for the amount of additives to be added to media of M. smegmatis, as well as markers’ colors to denote their presence.
Preparing 0.2% Glucose Agar Plates
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Prepare 0.2% glucose agar plates for growing M. smegmatis.
Preparing MBTA
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Prepare MBTA stock for later use in plating bacterial lawns.
Growing Mycobacterium smegmatis mc2 155
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To grow and maintain a plate stock of M. smegmatis mc2155, obtain a smooth starter culture for amplification using Tween®80, and grow bacteria for experiments.
Growing Mycobacterium smegmatis stlr 02
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To grow and maintain a plate stock of M. smegmatis stlr 02, obtain a smooth starter culture for amplification using Tween®80, and grow bacteria for experiments.