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Toolbox: Protocols and Information
Toolbox

Toolbox

The Toolbox was created to house those procedures that can be used in various parts of the workflow.
Protocol TitleDescription/Goal
Using a Gel-Doc Camera to Take Plaque Pictures
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To keep a visual record of plaque morphology using a GelDoc XR+ System.
Notebooks
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Measuring DNA Concentration by NanoDrop
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To quantify the amount of DNA in a phage or genomic DNA sample.
Picking a Plaque
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To obtain a liquid sample of phage from a plaque (or putative plaque) on a lawn of bacteria.
Making Serial Dilutions
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Create standard dilutions of a phage sample in order to perform plaque assays or titers.
Taking Plaque Pictures
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To keep a visual record of findings.
Titering
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To quantify the number of phage in a sample expressed as PFU (plaque forming unit)/mL.
Controls
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To ascertain whether the plaque detection system is working.
Obtaining GPS Coordinates
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Making Thumbnails for PhagesDB
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Describes how to select, crop, and prepare good pictures for use as thumbnail images on PhagesDB.org.
Lysate Archive Protocols
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If you submit information about a phage to PhagesDB.org, please submit a sample of the lysate to our archive inventory. If you are a member of the SEA-PHAGES program, the requested protocol for those samples is found here. Important changes for the 2015-16 school year are: 1. if you already have an MTA on file, there is no need for another. 2. We will send bar-coded tubes to all SEA-PHAGES members on October 12, 2015 if you enter enrollment numbers and shipping address at seaphages.org. Note: Complete Archiving Kits will only be shipped to Cohort 8 schools. All others will receive tubes and boxes, please evaluate and order appropriately. Please review complete protocol before processing archive samples. Protocol was modified August, 2015. Video Protocol