CDS 1 - 525
  /gene="1"
  /product="gp1"
  /function="hypothetical protein"
  /locus tag="Blage_1"
    /note=Original Glimmer call @bp 1 has strength 14.82; Genemark calls start at 1
    /note=SSC: 1-525 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein FDJ36_gp01 [Microbacterium phage Ilzat] ],,NCBI, q1:s1 100.0% 4.58426E-121 GAP: 0 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.761, -5.135095111895543, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDJ36_gp01 [Microbacterium phage Ilzat] ],,YP_009623165,100.0,4.58426E-121 SIF-HHPRED: 38-mer peptide; De Novo design, Covalent constrained peptide, DE NOVO PROTEIN; NMR {N/A},,,5WOD_A,14.9425,24.1 SIF-Syn: None
    /note=Start: 1
    /note=Gl/Gm favored: Yes
    /note=Gap: 184
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=1.8  Final=-5.1  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: NKF, no matches with an e value below e-7 
    /note=SIF-HHPRED: NKF, No viable hits over 90% probability, top hit 24% prob

CDS 522 - 1922
  /gene="2"
  /product="gp2"
  /function="terminase"
  /locus tag="Blage_2"
    /note=Original Glimmer call @bp 522 has strength 10.3; Genemark calls start at 522
    /note=SSC: 522-1922 CP: yes SCS: both ST: SS BLAST-Start: [terminase large subunit [Microbacterium phage Ilzat] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.908, -4.757883910852364, no F: terminase SIF-BLAST: ,,[terminase large subunit [Microbacterium phage Ilzat] ],,YP_009623166,100.0,0.0 SIF-HHPRED: Terminase, large subunit; phage defense, pattern-recognition receptor, nlr, stand, atpase, ANTIVIRAL PROTEIN; HET: ATP;{Salmonella enterica},,,8DGC_F,97.4249,100.0 SIF-Syn: yes, upstream of portal in Axipup and other EA1 phages
    /note=Start: 522
    /note=Gl/Gm favored: Yes
    /note=Gap: -4
    /note=Cp: Yes but some CP past the stop codon
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z = 1.9 Final = -4.8 Best = Yes
    /note=Q1s1: Yes 
    /note=SIF-BLASTp: terminase, Axipup, eval= 0, 100% alignment
    /note=SIF-HHPRED: terminase large subunit, E coli phage Phi-V1, 8DGC_F, 97% coverage, 100% probability

CDS 1953 - 3350
  /gene="3"
  /product="gp3"
  /function="portal protein"
  /locus tag="Blage_3"
    /note=Original Glimmer call @bp 1953 has strength 15.89; Genemark calls start at 1953
    /note=SSC: 1953-3350 CP: yes SCS: both ST: SS BLAST-Start: [portal protein [Microbacterium phage Den3] ],,NCBI, q1:s1 100.0% 0.0 GAP: 30 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.882, -2.5823297389851954, yes F: portal protein SIF-BLAST: ,,[portal protein [Microbacterium phage Den3] ],,QDP43993,100.0,0.0 SIF-HHPRED: Portal protein; Bacteriophage, SPP1, Portal Protein, Head completion proteins, Connector Complex, DNA Channel, VIRAL PROTEIN; 2.7A {Bacillus subtilis},,,7Z4W_D,90.1075,99.9 SIF-Syn: yes, downstream of terminase and upstream of MuF-like minor capsid protein
    /note=start: 1953
    /note=Gl/Gm favored: Both 
    /note=Gap: 30 bp gap
    /note=CP: yes
    /note=most annotated: Yes
    /note=most conserved: Yes
    /note=SD Z= 2.9 Final= -2.6, best score
    /note=Q1:S1: yes
    /note=SIF-BLASTp: portal, Den3, eval= 0, 100% alignment
    /note=SIF-HHPRED: portal, Bacillus phage SPP1, 7Z4W_D, 90% coverage, 99.9% probability

CDS 3356 - 4024
  /gene="4"
  /product="gp4"
  /function="hypothetical protein"
  /locus tag="Blage_4"
    /note=Original Glimmer call @bp 3356 has strength 11.81; Genemark calls start at 3356
    /note=SSC: 3356-4024 CP: yes SCS: both ST: SS BLAST-Start: [head morphogenesis [Microbacterium phage Ilzat] ],,NCBI, q1:s1 100.0% 4.6179E-162 GAP: 5 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.087, -4.645163458565996, no F: hypothetical protein SIF-BLAST: ,,[head morphogenesis [Microbacterium phage Ilzat] ],,YP_009623168,100.0,4.6179E-162 SIF-HHPRED:  SIF-Syn: none
    /note=start: 3356
    /note=Gl/Gm favored: Both 
    /note=Gap: 5
    /note=CP: yes
    /note=most annotated: Yes
    /note=most conserved: Yes
    /note=SD Z= 2.1 Final= -4.6, best score
    /note=Q1:S1: yes
    /note=SIF-BLASTp: MuF-like minor capsid, Axipup, eval= 0, 100% alignment
    /note=SIF-HHPRED: phage mu F,  PF04233.17, 43% coverage, 99.7% probability
    /note=Note- functions list from Seaphages no longer calls MuF-like proteins so calling NKF

CDS 4108 - 4668
  /gene="5"
  /product="gp5"
  /function="scaffolding protein"
  /locus tag="Blage_5"
    /note=Original Glimmer call @bp 4108 has strength 11.47; Genemark calls start at 4108
    /note=SSC: 4108-4668 CP: yes SCS: both ST: SS BLAST-Start: [scaffolding protein [Microbacterium phage AxiPup] ],,NCBI, q1:s1 100.0% 7.90825E-131 GAP: 83 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.007, -3.222210725506937, yes F: scaffolding protein SIF-BLAST: ,,[scaffolding protein [Microbacterium phage AxiPup] ],,AUX82654,100.0,7.90825E-131 SIF-HHPRED: PROTEIN GRPE; CHAPERONE, CHAPERONE CYCLE, COMPLEMENTARY ASSAY; 4.094A {GEOBACILLUS KAUSTOPHILUS},,,4ANI_B,78.4946,92.7 SIF-Syn: yes, upstream of major capsid and 2 genes downstream of portal
    /note=start: 4108
    /note=Gl/Gm favored: Both 
    /note=Gap: 83
    /note=CP: yes
    /note=most annotated: Yes
    /note=most conserved: Yes
    /note=SD Z= 3.0 Final= -3.2, best score
    /note=Q1:S1: yes
    /note=SIF-BLAST: scaffold, Axipup, eval= 8e-131, 100% alignment
    /note=SIF-HHPRED: chaperone protein,  Geobacillus, 4ANI_B, 78% coverage, 93% probability

CDS 4708 - 5634
  /gene="6"
  /product="gp6"
  /function="major capsid protein"
  /locus tag="Blage_6"
    /note=Original Glimmer call @bp 4708 has strength 18.11; Genemark calls start at 4708
    /note=SSC: 4708-5634 CP: yes SCS: both ST: SS BLAST-Start: [major capsid protein [Microbacterium phage MonChoix] ],,NCBI, q1:s1 100.0% 0.0 GAP: 39 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.174, -2.305049668942183, yes F: major capsid protein SIF-BLAST: ,,[major capsid protein [Microbacterium phage MonChoix] ],,YP_009908645,100.0,0.0 SIF-HHPRED: major capsid protein; acne, bacteriophage, HK97-like, VIRUS; 3.7A {Propionibacterium phage PA6},,,3JB5_F,94.1558,100.0 SIF-Syn: yes downstream of scaffolding protein in axipup
    /note=start: 4708
    /note=Gl/Gm favored: Both 
    /note=Gap: 39
    /note=CP: yes
    /note=most annotated: No, does not have the most annotated start
    /note=most conserved: Yes for EA1s
    /note=SD Z= 3.2 Final= -2.3, best score
    /note=Q1:S1: yes
    /note=SIF-BLASTp: major capsid, Monchoix, eval= 0, 100% alignment
    /note=SIF-HHPRED: major capsid, Propionibacteria phage PAS, 94% coverage, 100% probability

CDS 5631 - 5879
  /gene="7"
  /product="gp7"
  /function="hypothetical protein"
  /locus tag="Blage_7"
    /note=Original Glimmer call @bp 5631 has strength 8.35; Genemark calls start at 5631
    /note=SSC: 5631-5879 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_KNOX_7 [Microbacterium phage Knox] ],,NCBI, q1:s1 100.0% 2.56416E-47 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.868, -4.9056006736197375, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_KNOX_7 [Microbacterium phage Knox] ],,AUX82968,100.0,2.56416E-47 SIF-HHPRED: V-type proton ATPase subunit G; V-ATPase, rotary ATPase, complex, MEMBRANE PROTEIN; 3.9A {Citrus limon},,,7UWB_J,51.2195,69.2 SIF-Syn: none
    /note=start: 5631
    /note=Gl/Gm favored: Both 
    /note=Gap: -4
    /note=CP: no, tiny peak not included but is longest orf
    /note=most annotated: yes
    /note=most conserved: Yes 
    /note=SD Z= 1.9 Final= -4.9, best score
    /note=Q1:S1: yes
    /note=SIF-BLASTp: NKF, no hits with an e-value below 10-7
    /note=SIF-HHPRED: NKF, no supporting hits with probability above 90%, top hit 69%

CDS 5951 - 6085
  /gene="8"
  /product="gp8"
  /function="hypothetical protein"
  /locus tag="Blage_8"
    /note=Original Glimmer call @bp 5951 has strength 15.9; Genemark calls start at 5942
    /note=SSC: 5951-6085 CP: yes SCS: both-gl ST: NI BLAST-Start: [hypothetical protein PBI_LUDGATE_8 [Microbacterium phage Ludgate] ],,NCBI, q1:s1 100.0% 9.04422E-23 GAP: 71 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.947, -2.794070146961553, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_LUDGATE_8 [Microbacterium phage Ludgate] ],,AUX83032,100.0,9.04422E-23 SIF-HHPRED: g.41.13.1 (A:143-178) Hypothetical protein Ta0289 C-terminal domain {Thermoplasma acidophilum [TaxId: 2303]} | CLASS: Small proteins, FOLD: Rubredoxin-like, SUPFAM: Hypothetical protein Ta0289 C-terminal domain, FAM: Hypothetical protein Ta0289 C-terminal domain,,,SCOP_d1pvma3,40.9091,40.0 SIF-Syn: none
    /note=start: 5951
    /note=Gl/Gm favored: Glimmer
    /note=Gap: 71
    /note=CP: yes
    /note=most annotated: no but inconclusive 
    /note=most conserved: NI
    /note=SD Z= 2.9 Final= -2.8, best score
    /note=Q1:S1: yes
    /note=SIF-BLASTp: NKF, no hits with an e-value below 10-7
    /note=SIF-HHPRED: NKF, no supporting hits with probability above 90%, top hit 40%

CDS 6114 - 6527
  /gene="9"
  /product="gp9"
  /function="hypothetical protein"
  /locus tag="Blage_9"
    /note=Original Glimmer call @bp 6114 has strength 9.88; Genemark calls start at 6114
    /note=SSC: 6114-6527 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_BIGREDCLIFFORD_9 [Microbacterium phage BigRedClifford]],,NCBI, q1:s1 100.0% 5.95296E-96 GAP: 28 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.947, -2.794070146961553, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_BIGREDCLIFFORD_9 [Microbacterium phage BigRedClifford]],,QKY79252,100.0,5.95296E-96 SIF-HHPRED: RNA polymerase sigma factor RpoN; Sigma-54, sigma factors, solution structure, transcription, RNA polymerase; NMR {Aquifex aeolicus},,,2AHQ_A,21.8978,72.0 SIF-Syn: none
    /note=start: 6114
    /note=Gl/Gm favored: Both 
    /note=Gap: 28
    /note=CP: yes
    /note=most annotated: yes
    /note=most conserved: Yes 
    /note=SD Z= 2.9 Final= -2.8, best score
    /note=Q1:S1: yes
    /note=SIF-BLASTp: NKF, no hits with an e-value below 10-7
    /note=SIF-HHPRED: NKF, no supporting hits with probability above 90%, top hit 72%

CDS 6493 - 6906
  /gene="10"
  /product="gp10"
  /function="hypothetical protein"
  /locus tag="Blage_10"
    /note=Original Glimmer call @bp 6493 has strength 12.9; Genemark calls start at 6505
    /note=SSC: 6493-6906 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein PBI_AXIPUP_10 [Microbacterium phage AxiPup] ],,NCBI, q1:s1 100.0% 1.51898E-91 GAP: -35 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.655, -3.0713502170045657, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_AXIPUP_10 [Microbacterium phage AxiPup] ],,AUX82659,100.0,1.51898E-91 SIF-HHPRED: Gifsy-2 prophage ATP-binding sugar transporter-like protein; beta barrel, 4 helix bundle, Structural Genomics, PSI-2, Protein Structure Initiative, Midwest Center for Structural Genomics; HET: MSE; 2.7A {Salmonella typhimurium LT2} SCOP: b.106.1.2, l.1.1.1,,,2PP6_A,80.292,70.4 SIF-Syn: none
    /note=start: 6493
    /note=Gl/Gm favored: Both 
    /note=Gap: -35
    /note=CP: yes
    /note=most annotated: yes
    /note=most conserved: Yes 
    /note=SD Z= 2.7 Final= -3.1, best score
    /note=Q1:S1: yes
    /note=SIF-BLASTp: NKF, no hits with an e-value below 10-7
    /note=SIF-HHPRED: NKF, no supporting hits with probability above 90%, top hit 70.4%

CDS 6903 - 7244
  /gene="11"
  /product="gp11"
  /function="hypothetical protein"
  /locus tag="Blage_11"
    /note=Original Glimmer call @bp 6903 has strength 13.69; Genemark calls start at 6903
    /note=SSC: 6903-7244 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_AXIPUP_11 [Microbacterium phage AxiPup] ],,NCBI, q1:s1 100.0% 1.26537E-75 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.947, -2.583959800616441, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_AXIPUP_11 [Microbacterium phage AxiPup] ],,AUX82660,100.0,1.26537E-75 SIF-HHPRED: Minor_capsid_2 ; Minor capsid protein,,,PF11114.11,84.9557,99.6 SIF-Syn: none
    /note=start: 6903
    /note=Gl/Gm favored: Both 
    /note=Gap: -4
    /note=CP: yes
    /note=most annotated: yes
    /note=most conserved: Yes 
    /note=SD Z= 2.9 Final= -2.5, best score
    /note=Q1:S1: yes
    /note=SIF-BLASTp: minor capsid, HerculesXL, 76% alignment, e-value= 8.7x10-37, no other phages called it and no PDB evidence
    /note=SIF-HHPRED: minor capsid, PF11114.11, 99.6% probability, 85% coverage....inconclusive according to Hendrix lab info from the forum so NKF

CDS 7247 - 7606
  /gene="12"
  /product="gp12"
  /function="tail terminator"
  /locus tag="Blage_12"
    /note=Original Glimmer call @bp 7247 has strength 14.87; Genemark calls start at 7247
    /note=SSC: 7247-7606 CP: yes SCS: both ST: SS BLAST-Start: [tail terminator [Microbacterium phage AxiPup] ],,NCBI, q1:s1 100.0% 1.63819E-79 GAP: 2 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.299, -3.978223840957539, yes F: tail terminator SIF-BLAST: ,,[tail terminator [Microbacterium phage AxiPup] ],,AUX82661,100.0,1.63819E-79 SIF-HHPRED: TAIL-TO-HEAD JOINING PROTEIN GP17; VIRAL PROTEIN, VIRAL INFECTION, TAILED BACTERIOPHAGE, SIPHOVIRIDAE, SPP1, VIRAL ASSEMBLY, HEAD-TO-TAIL INTERFACE, DNA GATEKEEPER, ALLOSTERIC MECHANISM; 7.2A {BACILLUS PHAGE SPP1},,,5A21_G,73.1092,98.2 SIF-Syn: none but two upstream of major tail protein
    /note=start: 7247
    /note=Gl/Gm favored: Both 
    /note=Gap: 2
    /note=CP: yes
    /note=most annotated: yes
    /note=most conserved: Yes 
    /note=SD Z= 2.3 Final= -4, best score
    /note=Q1:S1: yes
    /note=SIF-BLASTp: tail terminator, Axipup, e-value=e-79, 100% alignment
    /note=SIF-HHPRED: head to tail joining protein, Bacillus phage SPP1, 98% probability, 73% coverage
    /note=
    /note=Note: Can call as tail terminator since we have a functional HHPRED hit with 5A21_G

CDS 7603 - 7839
  /gene="13"
  /product="gp13"
  /function="hypothetical protein"
  /locus tag="Blage_13"
    /note=Original Glimmer call @bp 7603 has strength 14.02; Genemark calls start at 7603
    /note=SSC: 7603-7839 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_AXIPUP_13 [Microbacterium phage AxiPup] ],,NCBI, q1:s1 100.0% 6.54542E-49 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.101, -2.17469248771465, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_AXIPUP_13 [Microbacterium phage AxiPup] ],,AUX82662,100.0,6.54542E-49 SIF-HHPRED: RNase_II_C_S1 ; RNase II-type exonuclease C-terminal S1 domain,,,PF18614.4,47.4359,66.7 SIF-Syn: none
    /note=start: 7603
    /note=Gl/Gm favored: Both 
    /note=Gap: -4
    /note=CP: yes
    /note=most annotated: yes
    /note=most conserved: Yes 
    /note=SD Z= 3.1 Final= -2.2, best score
    /note=Q1:S1: yes
    /note=SIF-BLASTp: NKF, no hits with an e-value below 10-7
    /note=SIF-HHPRED: NKF, no supporting hits with probability above 90%, top hit 67%

CDS 7854 - 8351
  /gene="14"
  /product="gp14"
  /function="major tail protein"
  /locus tag="Blage_14"
    /note=Original Glimmer call @bp 7854 has strength 14.52; Genemark calls start at 7854
    /note=SSC: 7854-8351 CP: yes SCS: both ST: SS BLAST-Start: [major tail protein [Microbacterium phage AxiPup] ],,NCBI, q1:s1 100.0% 2.14785E-114 GAP: 14 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.853, -2.6453814847322845, yes F: major tail protein SIF-BLAST: ,,[major tail protein [Microbacterium phage AxiPup] ],,AUX82663,100.0,2.14785E-114 SIF-HHPRED: Phage_TTP_11 ; Phage tail tube, TTP, lambda-like,,,PF16460.8,66.0606,88.6 SIF-Syn: upstream of tail assembly chaperone in Axipup
    /note=start: 7854
    /note=Gl/Gm favored: Both 
    /note=Gap: 14
    /note=CP: yes
    /note=most annotated: yes
    /note=most conserved: Yes 
    /note=SD Z= 2.9 Final= -2.6, best score
    /note=Q1:S1: yes
    /note=SIF-BLASTp: major tail protein, Axipup, 100% alignment, eval= e-114
    /note=SIF-HHPRED: NKF, no supporting hits with probability above 90%, top hit 88.6% with phage tail tube protein pfam

CDS 8382 - 8951
  /gene="15"
  /product="gp15"
  /function="tail assembly chaperone"
  /locus tag="Blage_15"
    /note=Original Glimmer call @bp 8382 has strength 14.37; Genemark calls start at 8382
    /note=SSC: 8382-8951 CP: yes SCS: both ST: SS BLAST-Start: [tail assembly chaperone [Microbacterium phage BigRedClifford]],,NCBI, q1:s1 100.0% 4.01475E-135 GAP: 30 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.947, -2.794070146961553, yes F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Microbacterium phage BigRedClifford]],,QKY79258,100.0,4.01475E-135 SIF-HHPRED: NUCLEAR DISTRIBUTION PROTEIN NUDE-LIKE 1; DEVELOPMENTAL PROTEIN, NUCLEAR PROTEIN, NEUROGENESIS, CYTOSKELETON, LIS1 BINDING, DIFFERENTIATION, PHOSPHORYLATION, TRANSPORT, MICROTUBULE, MICROTUBULE ASSOCIATED MIGRATION; HET: MSE; 2.24A {RATTUS NORVEGICUS},,,2V71_A,36.5079,65.3 SIF-Syn: yes, upstream of tail assembly chaperone in AxiPup
    /note=start: 8382
    /note=Gl/Gm favored: Both 
    /note=Gap: 30
    /note=CP: yes
    /note=most annotated: yes
    /note=most conserved: Yes 
    /note=SD Z= 2.9 Final= -2.8, best score
    /note=Q1:S1: yes
    /note=SIF-BLASTp: tail assembly chaperone, BigRedClifford, 100% align, eval = e-135
    /note=SIF-HHPRED: NKF, no supporting hits with probability above 90%, top hit 65%
    /note=Note: SEA forum indicates that the two genes between major tail and tapemeasure in EA1 phages are TAC, no frameshift present

CDS 8966 - 9337
  /gene="16"
  /product="gp16"
  /function="tail assembly chaperone"
  /locus tag="Blage_16"
    /note=Original Glimmer call @bp 8966 has strength 3.59; Genemark calls start at 8966
    /note=SSC: 8966-9337 CP: yes SCS: both ST: SS BLAST-Start: [tail assembly chaperone [Microbacterium phage HerculesXL]],,NCBI, q1:s197 100.0% 2.37542E-56 GAP: 14 bp gap LO: no RBS: Kibler 6, Karlin Medium, 0.835, -7.814653633833339, no F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Microbacterium phage HerculesXL]],,UVG33802,31.5625,2.37542E-56 SIF-HHPRED: NinF ; NinF protein,,,PF05810.15,21.9512,80.4 SIF-Syn: yes, downstream of tail assembly chaperone and upstream of tape measure protein in AxiPup
    /note=start: 8966
    /note=Gl/Gm favored: Both 
    /note=Gap: 14
    /note=CP: yes
    /note=most annotated: yes
    /note=most conserved: Yes 
    /note=SD Z= 0.8 Final= -7.8, best score: no
    /note=Q1:S1: yes
    /note=SIF-BLASTp: tail assembly chaperone, HerculesXL, 31% coverage, eval= e-56
    /note=SIF-HHPRED: NKF, no supporting hits with probability above 90%, top hit 80%
    /note=Note: SEA forum indicates that the two genes between major tail  and tapemeasure in EA1 phages are TAC, no frameshift present.

CDS 9362 - 11632
  /gene="17"
  /product="gp17"
  /function="tape measure protein"
  /locus tag="Blage_17"
    /note=Original Glimmer call @bp 9362 has strength 19.9; Genemark calls start at 9362
    /note=SSC: 9362-11632 CP: yes SCS: both ST: SS BLAST-Start: [tape measure protein [Microbacterium phage BigRedClifford]],,NCBI, q1:s1 100.0% 0.0 GAP: 24 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.174, -1.953940808934884, yes F: tape measure protein SIF-BLAST: ,,[tape measure protein [Microbacterium phage BigRedClifford]],,QKY79260,100.0,0.0 SIF-HHPRED: Tape Measure Protein, gp57; phage tail, tail tip, tape measure protein, VIRAL PROTEIN; 3.7A {Staphylococcus virus 80alpha},,,6V8I_CF,18.6508,100.0 SIF-Syn: Yes, downstream of tail assembly chaperone and upstream of minor tail protein in phage AxiPup
    /note=Start: 9362
    /note=Gl/Gm favored: Both
    /note=Gap: 24
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=3.2  Final=-2.0  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: Tape measure protein, BigRedClifford, 100% coverage, eval=0
    /note=SIF-HHPRED: Tape measure protein, Staphylococcus virus 80alpha (6V8I_CF), 100% probability, 99.8% coverage

CDS 11629 - 12402
  /gene="18"
  /product="gp18"
  /function="minor tail protein"
  /locus tag="Blage_18"
    /note=Original Glimmer call @bp 11629 has strength 14.97; Genemark calls start at 11629
    /note=SSC: 11629-12402 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Microbacterium phage BigRedClifford]],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.378, -3.8079208184165134, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage BigRedClifford]],,QKY79261,100.0,0.0 SIF-HHPRED: ORF46; Distal tail protein, Receptor-binding protein, Phage baseplate, host adsorption apparatus, genome injection device, VIRAL PROTEIN; 3.8A {Lactococcus phage TP901-1},,,4V96_AU,99.2218,99.9 SIF-Syn: Yes, downstream of minor tail protein in phage AxiPup.
    /note=Start: 11629
    /note=Gl/Gm favored: Both
    /note=Gap: -4
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.4  Final=-3.8  Best?=Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: Minor tail protein, BigRedClifford, 100% coverage, eval=0
    /note=SIF-HHPRED: Distal tail protein, Lactococcus phage TP901-1 (4V96_AU), probability 99.9%, coverage 99%

CDS 12402 - 14828
  /gene="19"
  /product="gp19"
  /function="minor tail protein"
  /locus tag="Blage_19"
    /note=Original Glimmer call @bp 12402 has strength 16.47; Genemark calls start at 12402
    /note=SSC: 12402-14828 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Microbacterium phage Oxtober96] ],,NCBI, q1:s1 100.0% 0.0 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.797, -2.7653702713535186, yes F: minor tail protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage Oxtober96] ],,QIN93951,100.0,0.0 SIF-HHPRED: Tail-Associated Lysin, gp59; phage tail, tail tip, tape measure protein, VIRAL PROTEIN; 3.7A {Staphylococcus virus 80alpha},,,6V8I_BE,39.2327,99.9 SIF-Syn: Yes, downstream of minor tail protein in BigRedClifford
    /note=Start: 12402
    /note=Gl/Gm favored: Both
    /note=Gap: -1
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.8  Final=-2.8  Best?=Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: Minor tail protein, BigRedClifford 100% coverage, eval=0
    /note=SIF-HHPRED: Tail-Associated Lysin, Staphylococcus virus 80alpha, 99.9% probability, 39% coverage

CDS 14828 - 15013
  /gene="20"
  /product="gp20"
  /function="minor tail protein"
  /locus tag="Blage_20"
    /note=Original Glimmer call @bp 14828 has strength 11.46; Genemark calls start at 14828
    /note=SSC: 14828-15013 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein FDJ36_gp20 [Microbacterium phage Ilzat] ],,NCBI, q1:s2 100.0% 2.51937E-35 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.462, -3.5660483139923818, yes F: minor tail protein SIF-BLAST: ,,[hypothetical protein FDJ36_gp20 [Microbacterium phage Ilzat] ],,YP_009623184,98.3871,2.51937E-35 SIF-HHPRED: CagX ; Conjugal transfer protein,,,PF03524.18,70.4918,87.9 SIF-Syn: between tapemeasure and endolysin
    /note=Start: 14828
    /note=Gl/Gm favored: Both
    /note=Gap: -1
    /note=Cp: yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.5  Final=-3.6  Best?=Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: no functional hits below e-7
    /note=SIF-HHPRED: a number of hits above 70% with hydrolases and based on SEA forum genes in the region between tapemeasure and endolysin should be called minor tail protein

CDS 15010 - 15606
  /gene="21"
  /product="gp21"
  /function="minor tail protein"
  /locus tag="Blage_21"
    /note=Original Glimmer call @bp 15010 has strength 8.21; Genemark calls start at 15010
    /note=SSC: 15010-15606 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Microbacterium phage Figueroism]],,NCBI, q1:s1 100.0% 1.29895E-139 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.009, -4.601664605840989, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage Figueroism]],,UVD40576,98.9899,1.29895E-139 SIF-HHPRED: XYLANASE; HYDROLASE, XYLOGLUCAN, CBM4-2, X2 L110F, CH-PI INTERACTION, ENGINEERED CONSTRUCT; HET: GLC, BGC, XYS; 1.0A {RHODOTHERMUS MARINUS} SCOP: b.18.1.14, l.1.1.1,,,4BJ0_A,24.2424,50.9 SIF-Syn: between tapemeasure and lysin
    /note=Start: 15010
    /note=Gl/Gm favored: Both
    /note=Gap: -4
    /note=Cp: Yes (but missing small portion at end)
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.0  Final=-4.6  Best?= yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: minor tail protein, Figueroism, 98% coverage and eval = e-139
    /note=SIF-HHPRED: No functional hits with a probability above 90% but hydrolases with 50% probability
    /note=Note: based on SEA forum genes in the region between tapemeasure and endolysin should be called minor tail protein

CDS 15606 - 16094
  /gene="22"
  /product="gp22"
  /function="minor tail protein"
  /locus tag="Blage_22"
    /note=Original Glimmer call @bp 15606 has strength 14.77; Genemark calls start at 15606
    /note=SSC: 15606-16094 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_PEEP_22 [Microbacterium phage Peep] ],,NCBI, q1:s1 100.0% 1.79838E-111 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.536, -3.389881189012514, yes F: minor tail protein SIF-BLAST: ,,[hypothetical protein PBI_PEEP_22 [Microbacterium phage Peep] ],,AUX83108,99.3827,1.79838E-111 SIF-HHPRED: Gp15 protein; Listeria, homotrimeric, receptor binding protein, Bacteriophage, VIRAL PROTEIN; HET: ACT, 1PE; 1.7A {Listeria phage PSA},,,6R5W_B,72.8395,99.4 SIF-Syn: between tapemeasure and endolysin
    /note=Start: 15606
    /note=Gl/Gm favored: Both
    /note=Gap: -1
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.5  Final=-3.4  Best?=Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: minor tail protein, Figuerism, 99% coverage, eval=-111
    /note=SIF-HHPRED: Receptor binding protein, Listeria phage PSA (6R5W_B), 73% coverage, 99% probability.

CDS 16094 - 18229
  /gene="23"
  /product="gp23"
  /function="minor tail protein"
  /locus tag="Blage_23"
    /note=Original Glimmer call @bp 16133 has strength 10.24; Genemark calls start at 16094
    /note=SSC: 16094-18229 CP: yes SCS: both-gm ST: SS BLAST-Start: [minor tail protein [Microbacterium phage Gargoyle] ],,NCBI, q1:s1 100.0% 0.0 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.99, -4.582905859694018, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage Gargoyle] ],,AWN03478,100.0,0.0 SIF-HHPRED: GH18_CFLE_spore_hydrolase; Cortical fragment-lytic enzyme (CFLE) is a peptidoglycan hydrolase involved in  bacterial endospore germination.,,,cd02874,46.4135,100.0 SIF-Syn: between tapemeasure and endolysin
    /note=Start: 16094
    /note=Gl/Gm favored: GeneMark Only
    /note=Gap: -1
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.0  Final=-4.6  Best?= No (but only second by 0.02)
    /note=Q1s1: Yes
    /note=SIF-BLASTp: Minor tail protein, Riyhil, 99.9% coverage, eval=0
    /note=SIF-HHPRED: Chitinase, Chromobacterium violaceum, 99.9 % probability, 47.2% coverage

CDS 18259 - 19065
  /gene="24"
  /product="gp24"
  /function="endolysin"
  /locus tag="Blage_24"
    /note=Original Glimmer call @bp 18259 has strength 12.76; Genemark calls start at 18259
    /note=SSC: 18259-19065 CP: yes SCS: both ST: SS BLAST-Start: [lysin A [Microbacterium phage Knox] ],,NCBI, q1:s1 100.0% 0.0 GAP: 29 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.947, -2.5052746077145835, yes F: endolysin SIF-BLAST: ,,[lysin A [Microbacterium phage Knox] ],,AUX82985,100.0,0.0 SIF-HHPRED: LysB4; endolysin, LAS type enzyme, L-Alanoyl D-Glutamate endopeptidase, HYDROLASE; 2.4A {Bacillus phage B4},,,6AKV_A,40.6716,98.9 SIF-Syn: Downstream of minor tail protein in AxiPup
    /note=Start: 18259
    /note=Gl/Gm favored: Both
    /note=Gap: 29
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.9  Final=-2.5  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: Lysin A, Knox , 100% alignment, eval=0
    /note=SIF-HHPRED: endolysin, B. subtilis phage B4, 6AKV_A, 99% probability, 40% coverage

CDS 19100 - 19489
  /gene="25"
  /product="gp25"
  /function="membrane protein"
  /locus tag="Blage_25"
    /note=Original Glimmer call @bp 19100 has strength 14.53; Genemark calls start at 19100
    /note=SSC: 19100-19489 CP: yes SCS: both ST: SS BLAST-Start: [membrane protein [Microbacterium phage Ilzat] ],,NCBI, q1:s1 100.0% 3.40859E-88 GAP: 34 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.947, -2.970161406017234, yes F: membrane protein SIF-BLAST: ,,[membrane protein [Microbacterium phage Ilzat] ],,YP_009623189,100.0,3.40859E-88 SIF-HHPRED: Outer capsid protein sigma-1; cell attachment protein, reovirus sigma1, coiled coil, beta-spiral repeat, VIRAL PROTEIN; 2.15A {Mammalian orthoreovirus 3 Dearing},,,6GAP_C,58.9147,90.5 SIF-Syn: upstream of holin and downstream of Lysin A in AxiPup
    /note=Start: 19100
    /note=Gl/Gm favored: Both
    /note=Gap: 34
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.9  Final=-2.9  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: Membrane protein, AxiPup, 100% alignment, eval=-88
    /note=SIF-HHPRED: Outer capsid protein sigma-1, Mammalian orthoreovirus 3 Dearing (6GAP_C), 90.5% probability, 59% coverage
    /note=Note: Two pieces of evidence used to call membrane protein from SOSUI and TMHMM

CDS 19494 - 19835
  /gene="26"
  /product="gp26"
  /function="holin"
  /locus tag="Blage_26"
    /note=Original Glimmer call @bp 19494 has strength 18.14; Genemark calls start at 19494
    /note=SSC: 19494-19835 CP: yes SCS: both ST: SS BLAST-Start: [holin [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 1.48707E-68 GAP: 4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.08, -2.297359520375101, yes F: holin SIF-BLAST: ,,[holin [Microbacterium phage Teagan] ],,YP_009908728,100.0,1.48707E-68 SIF-HHPRED: Phage_holin_5_1 ; Bacteriophage A118-like holin, Hol118,,,PF06946.14,73.4513,99.7 SIF-Syn: two genes downstream of lysin A in Axipup and other EA1 phages
    /note=Start: 19494
    /note=Gl/Gm favored: Both
    /note=Gap: 4
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=3.1  Final=-2.3  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: Holin, microbacterium phage Teagan, 100% coverage, eval=-68
    /note=SIF-HHPRED: Bacteriophage-like holin, Hol118, PF06946.14, 99.9% probability, 73.5% coverage
    /note=Note: has 3 TmHs

CDS 19895 - 20305
  /gene="27"
  /product="gp27"
  /function="hypothetical protein"
  /locus tag="Blage_27"
    /note=Original Glimmer call @bp 19895 has strength 11.83; Genemark calls start at 19895
    /note=SSC: 19895-20305 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein H3N90_gp27 [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 4.21819E-94 GAP: 59 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.062, -4.34663776219455, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein H3N90_gp27 [Microbacterium phage Teagan] ],,YP_009908729,100.0,4.21819E-94 SIF-HHPRED: DUF3016 ; Protein of unknown function (DUF3016),,,PF11454.11,33.8235,76.0 SIF-Syn: None
    /note=Start: 19895
    /note=Gl/Gm favored: Both
    /note=Gap: 59
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z= 2.1 Final=-4.3  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7
    /note=SIF-HHPRED: No functional hits above 90% probability, top hit 76% prob

CDS complement (20373 - 20561)
  /gene="28"
  /product="gp28"
  /function="hypothetical protein"
  /locus tag="Blage_28"
    /note=Original Glimmer call @bp 20561 has strength 19.12; Genemark calls start at 20561
    /note=SSC: 20561-20373 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein FDJ36_gp27 [Microbacterium phage Ilzat] ],,NCBI, q1:s1 100.0% 1.02868E-36 GAP: 70 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.947, -2.442961286954254, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDJ36_gp27 [Microbacterium phage Ilzat] ],,YP_009623191,100.0,1.02868E-36 SIF-HHPRED: DUF4846 ; Domain of unknown function (4846),,,PF16138.8,67.7419,78.9 SIF-Syn: None
    /note=Start: 20561
    /note=Gl/Gm favored: Both
    /note=Gap: 70 bp gap 
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.9  Final=-2.4  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 78.9% prob

CDS complement (20632 - 20802)
  /gene="29"
  /product="gp29"
  /function="hypothetical protein"
  /locus tag="Blage_29"
    /note=Original Glimmer call @bp 20784 has strength 15.45; Genemark calls start at 20784
    /note=SSC: 20802-20632 CP: yes SCS: both-cs ST: SS BLAST-Start: [hypothetical protein PBI_AXIPUP_29 [Microbacterium phage AxiPup] ],,NCBI, q1:s1 100.0% 1.28306E-33 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.469, -5.702527616004429, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_AXIPUP_29 [Microbacterium phage AxiPup] ],,AUX82678,100.0,1.28306E-33 SIF-HHPRED: 5-chloro-2-hydroxyhydroquinone dehydrochlorinase (TftG); LYASE; 1.75A {Burkholderia cepacia} SCOP: d.58.4.0,,,4LBH_A,58.9286,89.5 SIF-Syn: None
    /note=Start: 20802
    /note=Gl/Gm favored: Both
    /note=Gap: -4
    /note=Cp: Yes
    /note=Most Annotated: yes
    /note=Most Conserved: Yes
    /note=SD: Z=1.5  Final=-5.7  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 89.5% prob (PDB hit- 5-chloro-2-hydroxyhydroquinone dehydrochlorinase, Burkholderia cepacia)

CDS complement (20799 - 20957)
  /gene="30"
  /product="gp30"
  /function="hypothetical protein"
  /locus tag="Blage_30"
    /note=Original Glimmer call @bp 20957 has strength 10.18; Genemark calls start at 20957
    /note=SSC: 20957-20799 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_AXIPUP_30 [Microbacterium phage AxiPup] ],,NCBI, q1:s1 100.0% 3.03491E-28 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.254, -4.075451192669358, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_AXIPUP_30 [Microbacterium phage AxiPup] ],,AUX82679,100.0,3.03491E-28 SIF-HHPRED: PROTEIN (FLIG); FLAGELLAR MOTOR SWITCH PROTEIN, STRUCTURAL PROTEIN; 2.2A {Thermotoga maritima} SCOP: a.118.14.1,,,1QC7_A,84.6154,86.8 SIF-Syn: None
    /note=Start: 20957
    /note=Gl/Gm favored: Both
    /note=Gap: -4
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.3  Final=-4.0  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF- BLAST: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No functional hits with a probability above 90%, top hit 86.8% with flagellar motor switch protein

CDS complement (20954 - 21796)
  /gene="31"
  /product="gp31"
  /function="hypothetical protein"
  /locus tag="Blage_31"
    /note=Original Glimmer call @bp 21796 has strength 12.83; Genemark calls start at 21796
    /note=SSC: 21796-20954 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein FDJ36_gp30 [Microbacterium phage Ilzat] ],,NCBI, q1:s1 100.0% 0.0 GAP: 43 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.484, -6.242072432500853, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDJ36_gp30 [Microbacterium phage Ilzat] ],,YP_009623194,100.0,0.0 SIF-HHPRED: Antitermination protein; RNA polymerase, DNA Binding, transcription, Q-dependent antitermination, Q antitermination factor, GENE REGULATION, TRANSFERASE-DNA-RNA complex;{Escherichia coli},,,7UBN_Q,15.3571,95.1 SIF-Syn: None
    /note=Start: 21796
    /note=Gl/Gm favored: Both
    /note=Gap: 43 
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=1.5  Final=-6.2  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: ssDNA binding protein, Gardenstate, 97% coverage, eval= e-8
    /note=SIF-HHPRED: Antitermination protein (lambda phage), E. Coli (7UBJ_A), 15% coverage, 94.6% probability
    /note=Note: not calling SSB protein, because HHPRED hit has a low percent coverage and there is a lack of evidence supporting it being single stranded, as well as there only being one supporting BLAST hit

CDS complement (21840 - 22178)
  /gene="32"
  /product="gp32"
  /function="membrane protein"
  /locus tag="Blage_32"
    /note=Original Glimmer call @bp 22178 has strength 5.39; Genemark calls start at 22178
    /note=SSC: 22178-21840 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein H3N90_gp32 [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 1.02176E-74 GAP: 9 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.091, -2.213540211474171, yes F: membrane protein SIF-BLAST: ,,[hypothetical protein H3N90_gp32 [Microbacterium phage Teagan] ],,YP_009908734,100.0,1.02176E-74 SIF-HHPRED: Inner membrane lipoprotein YiaD; Dot/Icm, Secretion, T4SS, TRANSLOCASE; 4.6A {Legionella pneumophila},,,7MUY_BK,41.0714,93.7 SIF-Syn: none
    /note=Gl/Gm favored: Both
    /note=Gap: 9
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=3.1  Final=-2.2  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: SIF-BLASTp: No functional hits with an eval above -7 
    /note=SIF-HHPRED: Inner membrane lipoprotein, Legionella pneumophila, 41% coverage, 93.7% prob
    /note=Note: Two pieces of evidence used to call membrane protein from SOSUI and TMHMM

CDS complement (22188 - 22430)
  /gene="33"
  /product="gp33"
  /function="membrane protein"
  /locus tag="Blage_33"
    /note=Original Glimmer call @bp 22430 has strength 15.75; Genemark calls start at 22430
    /note=SSC: 22430-22188 CP: yes SCS: both ST: SS BLAST-Start: [membrane protein [Microbacterium phage BigRedClifford]],,NCBI, q1:s1 100.0% 1.21954E-49 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.035, -2.394485424036831, yes F: membrane protein SIF-BLAST: ,,[membrane protein [Microbacterium phage BigRedClifford]],,QKY79276,100.0,1.21954E-49 SIF-HHPRED: Regulatory peptide; Transmembrane helical complex, MEMBRANE PROTEIN; NMR {N/A},,,2MC7_A,28.75,80.3 SIF-Syn: Yes, upstream of DNA recombinase in Microbacterium phage AxiPup.
    /note=Start: 22430
    /note=Gl/Gm favored: Both
    /note=Gap: -4
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes (Called 100% of time when present)
    /note=SD: Z=3.0 Final=-2.4  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: Membrane protein, BigRedClifford (QKY79276), 100% probability, eval=-49 
    /note=SIF-HHPRED: Membrane protein, Salmonella enterica subsp. enterica serovar Typhimurium (2MC7_A), 80.3% probability, 28.8% coverage
    /note=Note: Three pieces of evidence used to call membrane protein from SOSUI (1) and TMHMM (2)

CDS complement (22427 - 24097)
  /gene="34"
  /product="gp34"
  /function="DNA primase/helicase"
  /locus tag="Blage_34"
    /note=Original Glimmer call @bp 24028 has strength 11.66; Genemark calls start at 24097
    /note=SSC: 24097-22427 CP: yes SCS: both-gm ST: SS BLAST-Start: [DNA recombinase [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 0.0 GAP: -25 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.017, -2.4329349954350334, yes F: DNA primase/helicase SIF-BLAST: ,,[DNA recombinase [Microbacterium phage Teagan] ],,YP_009908736,100.0,0.0 SIF-HHPRED: DNA primase/helicase; primase, helicase, dNTPase, DNA replication, TRANSFERASE; 3.45A {Enterobacteria phage T7} SCOP: c.37.1.11, e.13.1.2,,,1Q57_D,44.964,99.7 SIF-Syn: Yes, upstream of nuclease in AxiPup
    /note=Start: 24097
    /note=Gl/Gm favored: Gm
    /note=Gap: -25
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=3.0  Final=-2.4  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: DNA recombinase, Greys (QCG78236), 100% coverage, eval=0
    /note=SIF-HHPRED: DNA primase/helicase, Enterobacteria phage T7  (1Q57_D), 99.7% probability, 44.9% coverage.

CDS complement (24073 - 24366)
  /gene="35"
  /product="gp35"
  /function="nuclease"
  /locus tag="Blage_35"
    /note=Original Glimmer call @bp 24366 has strength 4.71; Genemark calls start at 24366
    /note=SSC: 24366-24073 CP: yes SCS: both ST: SS BLAST-Start: [endonuclease [Microbacterium phage Ilzat] ],,NCBI, q1:s1 100.0% 1.24499E-65 GAP: 3 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.142, -6.678258492274136, no F: nuclease SIF-BLAST: ,,[endonuclease [Microbacterium phage Ilzat] ],,YP_009623198,100.0,1.24499E-65 SIF-HHPRED: Nuclease; Nuclease, HYDROLASE; HET: SO4; 1.85A {Salmonella phage SETP3} SCOP: c.52.1.35,,,4QBN_A,94.8454,99.9 SIF-Syn: Yes, downstream of DNA recombinase in Microbacterium phage AxiPup.
    /note=Start: 24366
    /note=Gl/Gm favored: Both 
    /note=Gap: 3
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=1.1  Final=-6.7  Best?= No, but LORF
    /note=Q1s1: Yes
    /note=SIF-BLASTp: Nuclease, AxiPup & Knox (YP_009623198), 100% coverage, eval=-65
    /note=SIF-HHPRED: Nuclease, Salmonella phage SEPT3 (4QBN_A), 99.9% probability, 94.8% coverage

CDS complement (24370 - 25209)
  /gene="36"
  /product="gp36"
  /function="hypothetical protein"
  /locus tag="Blage_36"
    /note=Original Glimmer call @bp 25209 has strength 17.0; Genemark calls start at 25209
    /note=SSC: 25209-24370 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein H3N90_gp36 [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 0.0 GAP: 32 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.655, -3.2123487306667524, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein H3N90_gp36 [Microbacterium phage Teagan] ],,YP_009908738,100.0,0.0 SIF-HHPRED: Transcription termination factor Rho; transcription termination factor, RNA helicase, ATP-dependent, molecular motor, TRANSCRIPTION; HET: ATP;{Mycobacterium tuberculosis},,,7OQH_D,37.276,96.4 SIF-Syn: None
    /note=Start: 25209
    /note=Gl/Gm favored: Both
    /note=Gap: 32
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.7  Final=-3.2  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: Transcription termination factor, Mycobacterium tuberculosis (7OQH_D), 96.4% probability, 37.3 % coverage.

CDS complement (25242 - 25910)
  /gene="37"
  /product="gp37"
  /function="RecA-like DNA recombinase"
  /locus tag="Blage_37"
    /note=Original Glimmer call @bp 25910 has strength 14.06; Genemark calls start at 25910
    /note=SSC: 25910-25242 CP: yes SCS: both ST: SS BLAST-Start: [AAA-ATPase [Microbacterium phage Ilzat] ],,NCBI, q1:s1 100.0% 9.84616E-162 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.077, -4.455662434380964, no F: RecA-like DNA recombinase SIF-BLAST: ,,[AAA-ATPase [Microbacterium phage Ilzat] ],,YP_009623200,100.0,9.84616E-162 SIF-HHPRED: c.37.1.11 (A:15-282) RecA protein, ATPase-domain {Deinococcus radiodurans [TaxId: 1299]} | CLASS: Alpha and beta proteins (a/b), FOLD: P-loop containing nucleoside triphosphate hydrolases, SUPFAM: P-loop containing nucleoside triphosphate hydrolases, FAM: RecA protein-like (ATPase-domain),,,SCOP_d1xp8a1,75.2252,98.7 SIF-Syn: Yes, upstream of exonuclease in Mycobacterium phage AxiPup
    /note=Start: 25910
    /note=Gl/Gm favored: Both
    /note=Gap: -4
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.1  Final=-4.5  Best?= No, but LORF
    /note=Q1s1: Yes
    /note=SIF-BLASTp: AAA-ATPase, AxiPup and other similar EA1 phages (YP_009623200), 100% coverage, eval=-162
    /note=SIF-HHPRED: RecA protein, Mycobacterium tuberculosis (4PPF_A), 98.7% probability, 77.0% coverage

CDS complement (25907 - 27082)
  /gene="38"
  /product="gp38"
  /function="RecB-like exonuclease/helicase"
  /locus tag="Blage_38"
    /note=Original Glimmer call @bp 27082 has strength 11.11; Genemark calls start at 27091
    /note=SSC: 27082-25907 CP: yes SCS: both-gl ST: SS BLAST-Start: [exonuclease [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 0.0 GAP: -14 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.376, -3.670670413150579, no F: RecB-like exonuclease/helicase SIF-BLAST: ,,[exonuclease [Microbacterium phage Teagan] ],,YP_009908740,100.0,0.0 SIF-HHPRED: ATP-dependent DNA helicase (UvrD/REP); DNA, DNA BINDING PROTEIN, DNA BINDING PROTEIN-DNA complex; 3.5A {Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)},,,6PPU_A,83.6317,99.8 SIF-Syn: Yes, downstream of RecA-like recombinase and upstream of DNA polymerase I in WildNOut
    /note=Start: 2708
    /note=Gl/Gm favored: Gl
    /note=Gap: -14
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.4  Final=-3.7  Best?= Yes (by a margin of more than 1.0) 
    /note=Q1s1: Yes
    /note=SIF-BLASTp: Exonuclease, Axipup & Knox (YP_009908740), 100% coverage, eval=0
    /note=SIF-HHPRED: ATP dependent DNA helicase (6PPU_A), 99.8% probability, 84% coverage.
    /note=Note: has both helicase and nuclease domains based on HHpred

CDS complement (27069 - 28934)
  /gene="39"
  /product="gp39"
  /function="DNA polymerase I"
  /locus tag="Blage_39"
    /note=Original Glimmer call @bp 28934 has strength 12.56; Genemark calls start at 28925
    /note=SSC: 28934-27069 CP: yes SCS: both-gl ST: SS BLAST-Start: [DNA polymerase I [Microbacterium phage BigRedClifford]],,NCBI, q1:s1 100.0% 0.0 GAP: 623 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.873, -2.6013996449736907, yes F: DNA polymerase I SIF-BLAST: ,,[DNA polymerase I [Microbacterium phage BigRedClifford]],,QKY79282,100.0,0.0 SIF-HHPRED: DNA polymerase I; mycobacteria, DNA polymerase, Flap endonuclease, TRANSFERASE; 2.713A {Mycolicibacterium smegmatis},,,6VDE_A,99.0338,100.0 SIF-Syn: Yes, downstream of RecB-like exonuclease/helicase in WildNout
    /note=Start: 28934
    /note=Gl/Gm favored: Gl
    /note=Gap: 623
    /note=Cp: Yes
    /note=Most Annotated: yes
    /note=Most Conserved: yes
    /note=SD: Z=2.9  Final=-2.6  Best?= Yes by a large margin
    /note=Q1s1: Yes
    /note=SIF-BLASTp: DNA polymerase 1, BigRedClifford (QKY79282), 100% coverage, eval=0
    /note=SIF-HHPRED: DNA polymerase I (6VDE_A), M. smegmatis, 99% coverage, 100% prob
    /note=Note: no coding potential in large gap with possible start and stop codons

CDS 29558 - 29674
  /gene="40"
  /product="gp40"
  /locus tag="Blage_40"
    /note=Genemark calls start at 29558
    /note=SSC: 29558-29674 CP: no SCS: genemark ST: NI BLAST-Start:  GAP: 623 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.965, -5.383507109175471, no F:  SIF-BLAST:  SIF-HHPRED:  SIF-Syn: 
    /note=deleting because in forward direction and overlaps reverse genes

CDS complement (29134 - 29559)
  /gene="41"
  /product="gp41"
  /function="hypothetical protein"
  /locus tag="Blage_41"
    /note=Original Glimmer call @bp 29559 has strength 17.64; Genemark calls start at 29559
    /note=SSC: 29559-29134 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein FDJ36_gp39 [Microbacterium phage Ilzat] ],,NCBI, q1:s1 100.0% 2.43169E-91 GAP: 170 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.947, -2.442961286954254, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDJ36_gp39 [Microbacterium phage Ilzat] ],,YP_009623203,100.0,2.43169E-91 SIF-HHPRED: 60S ACIDIC RIBOSOMAL PROTEIN P2; RIBOSOMAL PROTEIN, RIBONUCLEOPROTEIN, RIBOSOME, TRANSLATION, DIMERIZATION, PHOSPHOPROTEIN; NMR {HOMO SAPIENS},,,2W1O_A,39.0071,83.2 SIF-Syn: None
    /note=Start: 29559
    /note=Gl/Gm favored: Both
    /note=Gap: 170
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.9  Final=-2.4  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 83.2% prob

CDS complement (29730 - 31118)
  /gene="42"
  /product="gp42"
  /function="DNA helicase"
  /locus tag="Blage_42"
    /note=Original Glimmer call @bp 31118 has strength 14.23; Genemark calls start at 31118
    /note=SSC: 31118-29730 CP: yes SCS: both ST: SS BLAST-Start: [DNA helicase [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 0.0 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.295, -3.9859767531965447, no F: DNA helicase SIF-BLAST: ,,[DNA helicase [Microbacterium phage Teagan] ],,YP_009908743,100.0,0.0 SIF-HHPRED: ATP-dependent DNA helicase uvsW; ATP-dependant helicase, T4-bacteriophage, Recombination, HYDROLASE; 2.7A {Enterobacteria phage T4},,,2OCA_A,98.0519,100.0 SIF-Syn: Yes, upstream of phosphoesterase in Microbacterium phage BigRedClifford.
    /note=Start: 31118
    /note=Gl/Gm favored: Both
    /note=Gap: -1
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.3  Final=4.0  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: DNA helicase, AxiPup (YP_009908743), 100% coverage, eval=0
    /note=SIF-HHPRED: ATP-dependent DNA helicase uvsW, Enterobacteria phage T4 (2OCA_A), 100% probability, 98.1% coverage

CDS complement (31118 - 31711)
  /gene="43"
  /product="gp43"
  /function="phosphoesterase"
  /locus tag="Blage_43"
    /note=Original Glimmer call @bp 31711 has strength 14.92; Genemark calls start at 31711
    /note=SSC: 31711-31118 CP: yes SCS: both ST: SS BLAST-Start: [RNA ligase [Microbacterium phage Ilzat] ],,NCBI, q1:s1 100.0% 2.88912E-140 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.946, -4.738143471880277, no F: phosphoesterase SIF-BLAST: ,,[RNA ligase [Microbacterium phage Ilzat] ],,YP_009623205,100.0,2.88912E-140 SIF-HHPRED: RNA 2`,3`-cyclic phosphodiesterase; RNA repair, CPDase, 2, 3 -cyclic phosphodiesterase, HYDROLASE; HET: SO4, GOL; 1.6A {Deinococcus radiodurans} SCOP: d.61.1.0,,,5H7E_A,80.203,99.7 SIF-Syn: Yes, downstream of DNA helicase and upstream of MazG-like nucleotide pyrophosphohydrolase in Microbacterium phage BigRedClifford.
    /note=Start: 31711
    /note=Gl/Gm favored: Both
    /note=Gap: -4
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes, called 100% of time when present
    /note=SD: Z=1.9 Final=-4.7  Best?= No, but is LORF
    /note=Q1s1: Yes
    /note=SIF-BLASTp: Phosphoesterase, Oxtober96(YP_00962305), 100% coverage, eval=-140
    /note=SIF-HHPRED: RNA 2’,3’-cyclic phosphodiesterase, Deinococcus radiodurans (5H7E_A), 99.7% probability, 80.2% coverage

CDS complement (31708 - 32004)
  /gene="44"
  /product="gp44"
  /function="hypothetical protein"
  /locus tag="Blage_44"
    /note=Original Glimmer call @bp 32004 has strength 13.71; Genemark calls start at 32004
    /note=SSC: 32004-31708 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_KNOX_43 [Microbacterium phage Knox] ],,NCBI, q1:s1 100.0% 1.14194E-60 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.947, -2.523003374675015, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_KNOX_43 [Microbacterium phage Knox] ],,AUX83004,100.0,1.14194E-60 SIF-HHPRED: HTH-TYPE TRANSCRIPTIONAL REGULATOR MGRA; TRANSCRIPTIONAL REGULATOR, MULTIDRUG RESISTANCE REGULATOR, VIRULENCE DETERMINANT, TRANSCRIPTIONAL FACTORS; HET: MSE; 2.8A {STAPHYLOCOCCUS AUREUS} SCOP: a.4.5.28, l.1.1.1,,,2BV6_A,39.7959,65.0 SIF-Syn: None
    /note=Start: 32004
    /note=Gl/Gm favored: Both
    /note=Gap: -1
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.9  Final=-2.5  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 65% prob

CDS complement (32004 - 32282)
  /gene="45"
  /product="gp45"
  /function="hypothetical protein"
  /locus tag="Blage_45"
    /note=Original Glimmer call @bp 32282 has strength 14.99; Genemark calls start at 32282
    /note=SSC: 32282-32004 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_KNOX_44 [Microbacterium phage Knox] ],,NCBI, q1:s1 100.0% 5.60566E-61 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.984, -4.59534204625343, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_KNOX_44 [Microbacterium phage Knox] ],,AUX83005,100.0,5.60566E-61 SIF-HHPRED: RcnB ; Nickel/cobalt transporter regulator,,,PF11776.11,11.9565,82.3 SIF-Syn: None
    /note=Start: 32282
    /note=Gl/Gm favored: Both
    /note=Gap: -1
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes, called 100% of the time when present
    /note=SD: Z=2.0  Final=-4.6  Best?= Yes 
    /note=Q1s1: Yes 
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 82.3% prob

CDS complement (32282 - 33088)
  /gene="46"
  /product="gp46"
  /function="MazG-like nucleotide pyrophosphohydrolase"
  /locus tag="Blage_46"
    /note=Original Glimmer call @bp 33088 has strength 18.93; Genemark calls start at 33088
    /note=SSC: 33088-32282 CP: yes SCS: both ST: SS BLAST-Start: [nucleotide pyrophosphohydrolase [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.679, -3.1604619823777065, no F: MazG-like nucleotide pyrophosphohydrolase SIF-BLAST: ,,[nucleotide pyrophosphohydrolase [Microbacterium phage Teagan] ],,YP_009908747,100.0,0.0 SIF-HHPRED: MazG-like pyrophosphohydrolase (MazZ); S-2L, MazG-like pyrophosphohydrolase, MazZ, VIRAL PROTEIN; HET: MN, DGI, SO4; 1.43A {Cyanophage S-2L},,,7ODY_B,56.3433,97.9 SIF-Syn: MazG-like nucleotide pyrophosphohydrolase upstream and downstream of conserved NKF in EA1 phages.
    /note=Start: 33088
    /note=Gl/Gm favored: Both
    /note=Gap: -4
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes, called 100% of time when present
    /note=SD: Z=2.7  Final=-3.2  Best?= Yes
    /note=Q1s1: Yes 
    /note=SIF-BLASTp: Nucleotide pyrophosphohydrolase, AxiPup (YP_009908747), 100% coverage, eval=0
    /note=SIF-HHPRED: MazG-like pyrophosphohydrolase, Cyanophage S-2L (7ODY_B), 97.9% probability, 56% coverage

CDS complement (33085 - 33810)
  /gene="47"
  /product="gp47"
  /function="hypothetical protein"
  /locus tag="Blage_47"
    /note=Original Glimmer call @bp 33810 has strength 10.67; Genemark calls start at 33810
    /note=SSC: 33810-33085 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein H3N90_gp46 [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 1.15532E-176 GAP: -23 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.264, -6.125680687170574, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein H3N90_gp46 [Microbacterium phage Teagan] ],,YP_009908748,100.0,1.15532E-176 SIF-HHPRED: T3SS_needle_E ; Type III secretion system, cytoplasmic E component of needle,,,PF08988.13,21.1618,59.8 SIF-Syn: None
    /note=Start: 33810
    /note=Gl/Gm favored: Both
    /note=Gap: -23
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=1.3  Final=-6.1  Best?=No chosen based on gap and its being the most annotated and conserved start
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 59.8% prob

CDS complement (33788 - 34369)
  /gene="48"
  /product="gp48"
  /function="thymidylate kinase"
  /locus tag="Blage_48"
    /note=Original Glimmer call @bp 34369 has strength 14.39; Genemark calls start at 34369
    /note=SSC: 34369-33788 CP: yes SCS: both ST: SS BLAST-Start: [thymidylate kinase [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 3.3265E-141 GAP: 12 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.185, -2.0720764396375664, yes F: thymidylate kinase SIF-BLAST: ,,[thymidylate kinase [Microbacterium phage Teagan] ],,YP_009908749,99.4819,3.3265E-141 SIF-HHPRED: Thymidylate kinase; TMK, kinase, thymidylate kinase, MRSA, pipiridine, transferase-transferase inhibitor complex; HET: T05; 1.55A {Staphylococcus aureus subsp. aureus} SCOP: c.37.1.0,,,4HLC_B,63.7306,99.5 SIF-Syn: Yes, upstream of glycosyltransferase in Microbacterium phage BigRedClifford.
    /note=Start: 34369
    /note=Gl/Gm favored: Both
    /note=Gap: 12 
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes, called 100% of time when present
    /note=SD: Z=3.2  Final=-2.1  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: Thymidylate kinase, Teagan (YP_009908749), 100% coverage, eval=-141
    /note=SIF-HHPRED: Thymidylate kinase, Stapylococcus aureus subspecies aureus (4HLC_B), 99.5% probability, 63.7% coverage

CDS complement (34382 - 35314)
  /gene="49"
  /product="gp49"
  /function="glycosyltransferase"
  /locus tag="Blage_49"
    /note=Original Glimmer call @bp 35314 has strength 14.25; Genemark calls start at 35314
    /note=SSC: 35314-34382 CP: yes SCS: both ST: SS BLAST-Start: [glycosyltransferase [Microbacterium phage Ilzat] ],,NCBI, q1:s1 100.0% 0.0 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.967, -6.157910251162862, no F: glycosyltransferase SIF-BLAST: ,,[glycosyltransferase [Microbacterium phage Ilzat] ],,YP_009623211,100.0,0.0 SIF-HHPRED: ATP synthase subunits region ORF 6; majastridin, ATPase operon, glycosyl transferase, Rossmann fold, sulphur SAD, TRANSFERASE; HET: GOL; 1.1A {Rhodobacter blasticus},,,2NXV_B,31.6129,99.4 SIF-Syn: Yes, downstream of thymidylate kinase in Microbacterium phage BigRedClifford.
    /note=Start: 35314
    /note=Gl/Gm favored: Both
    /note=Gap: -8
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.0  Final=-6.2  Best?=No, but LORF
    /note=Q1s1: Yes
    /note=SIF-BLASTp: Glycosyltransferase, AxiPup (YP_009623211), 100% coverage, eval=0
    /note=SIF-HHPRED: Glycosyltransferase, Mycobacterium tuberculosis (4FIX_A), 99% probability, 72% coverage

CDS complement (35307 - 35540)
  /gene="50"
  /product="gp50"
  /function="hypothetical protein"
  /locus tag="Blage_50"
    /note=Original Glimmer call @bp 35441 has strength 9.75; Genemark calls start at 35540
    /note=SSC: 35540-35307 CP: yes SCS: both-gm ST: NI BLAST-Start: [hypothetical protein H3N90_gp49 [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 2.4477E-51 GAP: 62 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.072, -4.325675514574479, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein H3N90_gp49 [Microbacterium phage Teagan] ],,YP_009908751,100.0,2.4477E-51 SIF-HHPRED: Protein prgH; FHA domain, CELL INVASION; 1.858A {Salmonella enterica subsp. enterica serovar Typhimurium},,,4G2S_B,31.1688,72.8 SIF-Syn: None
    /note=Start: 35540
    /note=Gl/Gm favored: Gm
    /note=Gap: 62
    /note=Cp: Yes
    /note=Most Annotated: no but only 2 out of 4 annotated, is LOR
    /note=Most Conserved: no but only 2 out of 4 annotated, is LOR
    /note=SD: Z=2.1  Final=-4.3  Best?= Yes
    /note=Q1s1: yes
    /note=SIF-BLASTp: NKF, no functional matches with an eval below -7 
    /note=SIF-HHPRED: NKF, no functional hits over 90% probability, top hit 72.8% prob

CDS complement (35603 - 35899)
  /gene="51"
  /product="gp51"
  /function="membrane protein"
  /locus tag="Blage_51"
    /note=Original Glimmer call @bp 35899 has strength 10.59; Genemark calls start at 35899
    /note=SSC: 35899-35603 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein FDJ36_gp49 [Microbacterium phage Ilzat] ],,NCBI, q1:s1 100.0% 2.1759E-61 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.101, -2.253377680616507, yes F: membrane protein SIF-BLAST: ,,[hypothetical protein FDJ36_gp49 [Microbacterium phage Ilzat] ],,YP_009623213,98.9796,2.1759E-61 SIF-HHPRED: DUF6479 ; Family of unknown function (DUF6479),,,PF20087.2,50.0,87.9 SIF-Syn: none
    /note=Start: 35899
    /note=Gl/Gm favored: Both
    /note=Gap: -4
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes, called 100% of time when present
    /note=SD: Z=3.1  Final= -2.2 Best?= Yes, only start available 
    /note=Q1s1: Yes
    /note=SIF-BLASTp: Membrane Protein, Clancy, 100% coverage, eval=-61
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 87.9% prob
    /note=Note: Two pieces of evidence used to call membrane protein from SOSUI and TMHMM

CDS complement (35896 - 36663)
  /gene="52"
  /product="gp52"
  /function="thymidylate synthase"
  /locus tag="Blage_52"
    /note=Original Glimmer call @bp 36663 has strength 14.23; Genemark calls start at 36663
    /note=SSC: 36663-35896 CP: yes SCS: both ST: SS BLAST-Start: [thymidylate synthase [Microbacterium phage Ilzat] ],,NCBI, q1:s1 100.0% 0.0 GAP: 119 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.947, -2.523003374675015, yes F: thymidylate synthase SIF-BLAST: ,,[thymidylate synthase [Microbacterium phage Ilzat] ],,YP_009623214,100.0,0.0 SIF-HHPRED: Thymidylate synthase; structural genomics, PSI-Biology, Midwest Center for Structural Genomics, MCSG, Structures of Mtb Proteins Conferring Susceptibility to; HET: UMP; 2.71A {Staphylococcus aureus} SCOP: d.117.1.1,,,4DQ1_B,90.9804,100.0 SIF-Syn: near (3 genes away) thymidylate kinase in several phages
    /note=Start: 36663
    /note=Gl/Gm favored: Both
    /note=Gap: 119 2nd best gap, but the first (44) has a TTG start codon
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes, called 100% of time when present
    /note=SD: Z=2.9  Final=-2.5  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: Thymidylate synthase, AxiPup (YP_009623214), 100% coverage, eval=0
    /note=SIF-HHPRED: Thymidylate synthase, Staphylococcus aureus (4DQ1_B), 100% probability, 91% coverage

CDS complement (36783 - 37013)
  /gene="53"
  /product="gp53"
  /function="hypothetical protein"
  /locus tag="Blage_53"
    /note=Original Glimmer call @bp 36995 has strength 9.83; Genemark calls start at 36995
    /note=SSC: 37013-36783 CP: yes SCS: both-cs ST: SS BLAST-Start: [hypothetical protein PBI_KNOX_52 [Microbacterium phage Knox] ],,NCBI, q1:s1 100.0% 2.31988E-48 GAP: 60 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.588, -6.672171187999036, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_KNOX_52 [Microbacterium phage Knox] ],,AUX83013,100.0,2.31988E-48 SIF-HHPRED: LRC5; phycobilisome, Complex, PHOTOSYNTHESIS; HET: CYC, PEB, MEN, PUB; 2.8A {Porphyridium purpureum},,,6KGX_3H,76.3158,67.4 SIF-Syn: None
    /note=Start: 37013
    /note=Gl/Gm favored: no
    /note=Gap: 60
    /note=Cp: Yes
    /note=Most Annotated: yes
    /note=Most Conserved: Yes
    /note=SD: Z=1.6  Final=-6.7  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No functional hits with a probability above 90%, top hit 67.4%

CDS complement (37074 - 37298)
  /gene="54"
  /product="gp54"
  /function="hypothetical protein"
  /locus tag="Blage_54"
    /note=Original Glimmer call @bp 37298 has strength 18.83; Genemark calls start at 37379
    /note=SSC: 37298-37074 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein FDJ36_gp52 [Microbacterium phage Ilzat] ],,NCBI, q1:s1 100.0% 1.11884E-46 GAP: 64 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.101, -2.253377680616507, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDJ36_gp52 [Microbacterium phage Ilzat] ],,YP_009623216,100.0,1.11884E-46 SIF-HHPRED: Elongation Factor G; L11, EF-G, cryo-EM, 70S E.coli ribosome, GTP state, RIBOSOME; 18.0A {Escherichia coli} SCOP: i.1.1.1,,,1JQS_B,35.1351,49.2 SIF-Syn: None
    /note=Start: 37298
    /note=Gl/Gm favored: Gl
    /note=Gap: 64
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=3.1  Final=-2.3  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 49% prob

CDS complement (37363 - 37629)
  /gene="55"
  /product="gp55"
  /function="hypothetical protein"
  /locus tag="Blage_55"
    /note=Original Glimmer call @bp 37629 has strength 17.18; Genemark calls start at 37629
    /note=SSC: 37629-37363 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_BIGREDCLIFFORD_54 [Microbacterium phage BigRedClifford]],,NCBI, q1:s1 100.0% 4.08203E-53 GAP: 71 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.101, -2.1123791669543204, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_BIGREDCLIFFORD_54 [Microbacterium phage BigRedClifford]],,QKY79297,100.0,4.08203E-53 SIF-HHPRED: COILED-COIL PEPTIDE CC-PIL; DE NOVO PROTEIN; HET: PHI; 1.59A {N/A},,,4DZN_A,30.6818,84.9 SIF-Syn: None
    /note=Start: 37629
    /note=Gl/Gm favored: Both
    /note=Gap: 71
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes, called 100% of time when present
    /note=SD: Z=3.1  Final=-2.1  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 84.9% prob

CDS complement (37701 - 37997)
  /gene="56"
  /product="gp56"
  /function="hypothetical protein"
  /locus tag="Blage_56"
    /note=Original Glimmer call @bp 37997 has strength 16.08; Genemark calls start at 37997
    /note=SSC: 37997-37701 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein H3N90_gp55 [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 1.27227E-64 GAP: 91 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.035, -2.253486910374644, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein H3N90_gp55 [Microbacterium phage Teagan] ],,YP_009908757,100.0,1.27227E-64 SIF-HHPRED: Hydrogenase/urease nickel incorporation protein hypA; metallochaperone, hydrogenase, Metal-binding, Nickel, METAL-BINDING PROTEIN, METAL BINDING PROTEIN; HET: ZN; NMR {Helicobacter pylori},,,2KDX_A,24.4898,96.7 SIF-Syn: None
    /note=Start: 37997
    /note=Gl/Gm favored: Both
    /note=Gap: 91 (smallest gap)
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=3.0  Final=-2.3  Best?= Yes
    /note=Q1S1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: Zn ribbon containing protein, 4V9N_D5, H.pylori, 96.5% prob and 23% coverage; plus other zinc finger domain hits
    /note=conserved domain hit with zinc finger domain

CDS complement (38089 - 38283)
  /gene="57"
  /product="gp57"
  /function="hypothetical protein"
  /locus tag="Blage_57"
    /note=Original Glimmer call @bp 38283 has strength 11.93; Genemark calls start at 38283
    /note=SSC: 38283-38089 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein FDJ36_gp55 [Microbacterium phage Ilzat] ],,NCBI, q1:s1 100.0% 2.01918E-39 GAP: 38 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.675, -5.830816207622988, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDJ36_gp55 [Microbacterium phage Ilzat] ],,YP_009623219,100.0,2.01918E-39 SIF-HHPRED: EFh_HEF_CBN; EF-hand, calcium binding motif, found in Drosophila melanogaster calbindin-32 (CBN) and similar proteins.,,,cd16179,90.625,92.5 SIF-Syn: None
    /note=Start: 38283
    /note=Gl/Gm favored: Both
    /note=Gap: 38
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes, called 100% of time when present
    /note=SD: Z=1.7  Final=-5.8  Best?= No, but is LORF
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit cd16179- calcium binding motif in Drosophila, 92% prob

CDS complement (38322 - 38627)
  /gene="58"
  /product="gp58"
  /function="hypothetical protein"
  /locus tag="Blage_58"
    /note=Original Glimmer call @bp 38627 has strength 7.77; Genemark calls start at 38627
    /note=SSC: 38627-38322 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein H3N90_gp57 [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 7.065E-67 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.824, -5.386343183173524, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein H3N90_gp57 [Microbacterium phage Teagan] ],,YP_009908759,100.0,7.065E-67 SIF-HHPRED: FliG_M ; FliG middle domain,,,PF14841.9,39.604,66.0 SIF-Syn: None
    /note=Start: 38627
    /note=Gl/Gm favored: Both
    /note=Gap: -4
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes, called 100% of time when present
    /note=SD: Z=1.8  Final=-5.4  Best?= No, but preferred overlap
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 66%

CDS complement (38624 - 38809)
  /gene="59"
  /product="gp59"
  /function="hypothetical protein"
  /locus tag="Blage_59"
    /note=Original Glimmer call @bp 38809 has strength 10.71; Genemark calls start at 38809
    /note=SSC: 38809-38624 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein H3N90_gp58 [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 3.19984E-37 GAP: 1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.424, -5.78257814227218, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein H3N90_gp58 [Microbacterium phage Teagan] ],,YP_009908760,100.0,3.19984E-37 SIF-HHPRED: NPHI_C ; Nucleoside triphosphatase I C-terminal,,,PF08469.13,18.0328,77.0 SIF-Syn: None
    /note=Start: 38809
    /note=Gl/Gm favored: Both
    /note=Gap: 1
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=1.4  Final=-5.8  Best?= Yes only available start
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 77% prob

CDS complement (38811 - 39542)
  /gene="60"
  /product="gp60"
  /function="hypothetical protein"
  /locus tag="Blage_60"
    /note=Original Glimmer call @bp 39542 has strength 13.4; Genemark calls start at 39542
    /note=SSC: 39542-38811 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein H3N90_gp59 [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 2.28744E-175 GAP: 93 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.035, -2.253486910374644, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein H3N90_gp59 [Microbacterium phage Teagan] ],,YP_009908761,100.0,2.28744E-175 SIF-HHPRED: Histone_HNS ; H-NS histone family,,,PF00816.24,16.8724,74.3 SIF-Syn: None
    /note=Start: 39542
    /note=Gl/Gm favored: Both
    /note=Gap: 93
    /note=Cp: Yes
    /note=Most Annotated: Yes
    /note=Most Conserved: Yes
    /note=SD: Z=3.0  Final=-2.3  Best?= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 74.3% prob

CDS complement (39636 - 40022)
  /gene="61"
  /product="gp61"
  /function="hypothetical protein"
  /locus tag="Blage_61"
    /note=Original Glimmer call @bp 39974 has strength 19.98; Genemark calls start at 40022
    /note=SSC: 40022-39636 CP: yes SCS: both-gm ST: SS BLAST-Start: [hypothetical protein H3N90_gp60 [Microbacterium phage Teagan] ],,NCBI, q1:s1 100.0% 6.08986E-88 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.404, -3.9616183113819163, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein H3N90_gp60 [Microbacterium phage Teagan] ],,YP_009908762,100.0,6.08986E-88 SIF-HHPRED: c.115.1.1 (A:) Hypothetical protein MTH777 (MT0777) {Methanobacterium thermoautotrophicum [TaxId: 145262]} | CLASS: Alpha and beta proteins (a/b), FOLD: Hypothetical protein MTH777 (MT0777), SUPFAM: Hypothetical protein MTH777 (MT0777), FAM: Hypothetical protein MTH777 (MT0777),,,SCOP_d1kjna_,49.2188,64.4 SIF-Syn: none
    /note=Start: 40022
    /note=Gl/Gm favored: Gm
    /note=Gap: -4
    /note=Cp: Yes
    /note=Most Annotated: yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.4  Final=-4.0  Best?= Yes, preferred gap and LORF
    /note=Q1s1: yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 64% prob

CDS complement (40019 - 40378)
  /gene="62"
  /product="gp62"
  /function="hypothetical protein"
  /locus tag="Blage_62"
    /note=Original Glimmer call @bp 40276 has strength 6.13; Genemark calls start at 40378
    /note=SSC: 40378-40019 CP: yes SCS: both-gm ST: SS BLAST-Start: [hypothetical protein SEA_BIGREDCLIFFORD_61 [Microbacterium phage BigRedClifford]],,NCBI, q1:s1 100.0% 2.26326E-78 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.881, -5.563840133996638, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_BIGREDCLIFFORD_61 [Microbacterium phage BigRedClifford]],,QKY79304,100.0,2.26326E-78 SIF-HHPRED: SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT- CONTAINING PROTEIN 2; CHAPERONE-SIGNALING PROTEIN COMPLEX; 1.803A {SACCHAROMYCES CEREVISIAE},,,3ZDM_A,35.2941,78.4 SIF-Syn: none
    /note=Start: 40378
    /note=Gl/Gm favored: Gm
    /note=Gap: -4
    /note=Cp: Yes
    /note=Most Annotated: yes
    /note=Most Conserved: Yes
    /note=SD: Z=1.9  Final=-5.6  Best?= No
    /note=Q1s1: yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 78.4% prob

CDS complement (40375 - 40608)
  /gene="63"
  /product="gp63"
  /function="hypothetical protein"
  /locus tag="Blage_63"
    /note=Original Glimmer call @bp 40557 has strength 3.83; Genemark calls start at 40608
    /note=SSC: 40608-40375 CP: yes SCS: both-gm ST: SS BLAST-Start: [hypothetical protein PBI_NATTLES_61 [Microbacterium phage Nattles] ],,NCBI, q1:s1 100.0% 8.27376E-50 GAP: 561 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.894, -4.848494831654203, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_NATTLES_61 [Microbacterium phage Nattles] ],,AVJ50534,100.0,8.27376E-50 SIF-HHPRED: PDI_a_PDI_a`_C; PDIa family, C-terminal TRX domain (a`) subfamily; composed of the C-terminal redox active a` domains of PDI, ERp72, ERp57 (or ERp60) and EFP1.,,,cd02995,71.4286,64.0 SIF-Syn: none
    /note=Start: 40608
    /note=Gl/Gm favored: GM
    /note=Gap: 561
    /note=Cp: Yes
    /note=Most Annotated: yes
    /note=Most Conserved: Yes
    /note=SD: Z=1.9  Final=-4.9  Best?= no
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 64% prob

CDS 41170 - 41613
  /gene="64"
  /product="gp64"
  /function="hypothetical protein"
  /locus tag="Blage_64"
    /note=Original Glimmer call @bp 41170 has strength 14.38; Genemark calls start at 40984
    /note=SSC: 41170-41613 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein SEA_SONOFLEVI_62 [Microbacterium phage SonOfLevi]],,NCBI, q1:s1 100.0% 1.00532E-99 GAP: 561 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.947, -2.442961286954254, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_SONOFLEVI_62 [Microbacterium phage SonOfLevi]],,QHJ86258,100.0,1.00532E-99 SIF-HHPRED: Glyco_hydro_65C ; Glycosyl hydrolase family 65, C-terminal domain,,,PF03633.18,19.7279,39.2 SIF-Syn: none
    /note=Start: 41170
    /note=Gl/Gm favored: Gl
    /note=Gap: 561
    /note=Cp: Yes
    /note=Most Annotated: yes
    /note=Most Conserved: Yes
    /note=SD: Z=2.9  Final=-2.4  Best= Yes
    /note=Q1s1: Yes
    /note=SIF-BLASTp: No functional hits with an eval below -7 
    /note=SIF-HHPRED: No viable hits with a probability above 90%, top hit 39% prob