CDS complement (706 - 942) /gene="1" /product="gp1" /function="membrane protein" /locus tag="FrankDeliGuy_1" /note=Original Glimmer call @bp 942 has strength 6.1; Genemark calls start at 942 /note=SSC: 942-706 CP: yes SCS: both ST: SS BLAST-Start: [membrane protein [Microbacterium phage Wheelie]],,NCBI, q1:s1 100.0% 1.04956E-42 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.905, -2.9063687850157054, yes F: membrane protein SIF-BLAST: ,,[membrane protein [Microbacterium phage Wheelie]],,UVG33953,100.0,1.04956E-42 SIF-HHPRED: SIF-Syn: NKF- closely related phages have NKF /note=2 domains found by TMHMM and SOUSI /note=Start 942 has coding potential, supported by both glimmer and genemark, starterator, BLAST, has -4 bp gap, LORF and best RBS. Function is membrane protein shown by BLAST, TMHMM and SOUSI. CDS complement (939 - 1115) /gene="2" /product="gp2" /function="hypothetical protein" /locus tag="FrankDeliGuy_2" /note=Original Glimmer call @bp 1115 has strength 14.27; Genemark calls start at 1115 /note=SSC: 1115-939 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_LONELYSOIL_3 [Microbacterium phage Lonelysoil]],,NCBI, q1:s1 100.0% 5.26531E-32 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.905, -3.8154491356968365, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_LONELYSOIL_3 [Microbacterium phage Lonelysoil]],,WNM75114,100.0,5.26531E-32 SIF-HHPRED: SIF-Syn: NKF- closely related phages have NKF /note=Start 1115 has coding potential, is supported by both glimmer, genemark, starterator, many 1:1 hits in BLAST, -4 bp gap, best RBS. NKF determined by BLAST, HHPred, SYN, and Mem. CDS complement (1112 - 1459) /gene="3" /product="gp3" /function="hypothetical protein" /locus tag="FrankDeliGuy_3" /note=Original Glimmer call @bp 1459 has strength 3.99; Genemark calls start at 1498 /note=SSC: 1459-1112 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein SEA_BABYDOTZ_3 [Microbacterium phage BabyDotz] ],,NCBI, q1:s1 100.0% 1.01549E-77 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.409, -5.8787031795312865, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_BABYDOTZ_3 [Microbacterium phage BabyDotz] ],,WNT45258,100.0,1.01549E-77 SIF-HHPRED: SIF-Syn: NKF- closely related phages have NKF /note=Start 1459 is the most supported. It has coding potential, its supported by Glimmer, Starterator, has multiple 1:1 BLAST hits, has preffered gap of -4. Does not have LORF or best RBS, however the rest of the evidence outweighs this. NKF determined by BLAST, HHPred, SYN and Mem. CDS complement (1456 - 1641) /gene="4" /product="gp4" /function="hypothetical protein" /locus tag="FrankDeliGuy_4" /note=Original Glimmer call @bp 1641 has strength 5.49 /note=SSC: 1641-1456 CP: no SCS: glimmer ST: SS BLAST-Start: [hypothetical protein [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 2.62648E-32 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.956, -4.821320819095688, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein [Microbacterium phage Judebell]],,XCG97219,96.7213,2.62648E-32 SIF-HHPRED: SIF-Syn: NKF- closely related phages have NKF /note=Start 1641 is the most supported. It does not have coding potential, however it is supported by Glimmer, Starterator, has multiple blast hits, has perffered gap. It does not have LORF or best RBS but the other evidence outweighs this. NKF determined by BLAST, HHPred, and SYN. CDS complement (1638 - 1862) /gene="5" /product="gp5" /function="hypothetical protein" /locus tag="FrankDeliGuy_5" /note=Original Glimmer call @bp 1862 has strength 8.99; Genemark calls start at 1862 /note=SSC: 1862-1638 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein HOT29_gp007 [Microbacterium phage Squash] ],,NCBI, q1:s1 100.0% 3.02035E-42 GAP: 46 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.905, -2.9063687850157054, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein HOT29_gp007 [Microbacterium phage Squash] ],,YP_009801746,94.5946,3.02035E-42 SIF-HHPRED: SIF-Syn: Homologues in the neighborhood had no known functions /note=Start 1862 for FrankDeliGuy gene 6 is the most supported. It`s supported by both glimmer and genemark, having coding potential present. The starterator results have this start highly conserved within its genome, and also having the most manual annotations. NKF here as determined by Blast, HHPred, SYN and Mem. CDS complement (1909 - 2283) /gene="6" /product="gp6" /function="hypothetical protein" /locus tag="FrankDeliGuy_6" /note=Original Glimmer call @bp 2283 has strength 11.24; Genemark calls start at 2283 /note=SSC: 2283-1909 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_BABYDOTZ_6 [Microbacterium phage BabyDotz]],,NCBI, q1:s1 77.4194% 2.65489E-59 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.14, -2.417348306996335, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_BABYDOTZ_6 [Microbacterium phage BabyDotz]],,WNT45261,77.1186,2.65489E-59 SIF-HHPRED: SIF-Syn: Homologues in the neighborhood had no known function. /note=Start 2283 for FrankDeliGuy gene 7 is the most supported. It`s supported by both glimmer and genemark, having coding potential present. The starterator results have this start highly conserved within its genome, and also having the only manual annotation. NKF here as determined by Blast, HHPred, SYN and Mem. CDS complement (2280 - 2996) /gene="7" /product="gp7" /function="membrane protein" /locus tag="FrankDeliGuy_7" /note=Original Glimmer call @bp 2996 has strength 10.13; Genemark calls start at 2996 /note=SSC: 2996-2280 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_MASHLEY_6 [Microbacterium phage Mashley]],,NCBI, q1:s1 100.0% 7.17455E-172 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.758, -3.0713502170045657, yes F: membrane protein SIF-BLAST: ,,[hypothetical protein SEA_MASHLEY_6 [Microbacterium phage Mashley]],,QED11823,98.7395,7.17455E-172 SIF-HHPRED: Nicotinamide riboside transporter PnuC; membrane transport, vitamin transport, Nicotinamide riboside uptake, TRANSPORT PROTEIN; HET: BOG, MSE, NNR; 2.8A {Neisseria mucosa ATCC 25996},,,4QTN_A,31.9328,98.0 SIF-Syn: NKF - same pham as BabyDotz_6, Mashley_4, homologs have no known function. /note=Start 2996 for FrankDeliGuy gene 8 is the most supported. It`s supported by both glimmer and genemark, having coding potential present. The starterator results have this start highly conserved within its genome, and also having the most manual annotations. The gene`s function is a membrane protein, which is strongly supported by the SIF-BLAST, SIF-HHPred, SIF-SYN, and SIF-Mem, having three domains found by the DeepTMHMM and Sosui. CDS complement (2993 - 3514) /gene="8" /product="gp8" /function="DprA-like DNA processing chain A" /locus tag="FrankDeliGuy_8" /note=Original Glimmer call @bp 3517 has strength 10.2; Genemark calls start at 3514 /note=SSC: 3514-2993 CP: yes SCS: both-gm ST: NI BLAST-Start: [DprA-like DNA processing chain A [Microbacterium phage StrawberryJamm]],,NCBI, q1:s1 100.0% 1.09897E-118 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.804, -3.0377949497172554, yes F: DprA-like DNA processing chain A SIF-BLAST: ,,[DprA-like DNA processing chain A [Microbacterium phage StrawberryJamm]],,QWY80095,99.422,1.09897E-118 SIF-HHPRED: DNA processing protein DprA; SAM and Rossmann Fold, DNA processing protein A, DNA BINDING PROTEIN; HET: MSE, SO4; 2.7A {Streptococcus pneumoniae} SCOP: c.129.1.4, l.1.1.1, a.60.17.1,,,3UQZ_A,98.8439,99.5 SIF-Syn: DprA - like DNA processing chain A, same pham and position as Namago_10 and StrawberryJamm_12 /note=Start 3514 had the best evidence vs 3517, genemark supported the start as well as starterator because it had more manual annotations and it was highly conserved within its genome. The blast hits had better and more 1:1 alignments, and while the overlap is only 1bp(vs 3517 was 4bp overlap), it had the best RBS score. Overall the evidence pointed more towards 3514, which is not the selected start currently. According to the NCBI blast results and the HHpred results, alignment showed that the function is DprA-like DNA processing chain. This can also be backed up by looking at phamerator map, in which FrankDeliGuy_9 was in the same position and same pham as StrawberryJamm_12, which has a known funciton of Dpr-A-like DNA processing chain A. CDS complement (3514 - 3756) /gene="9" /product="gp9" /function="hypothetical protein" /locus tag="FrankDeliGuy_9" /note=Original Glimmer call @bp 3756 has strength 13.95; Genemark calls start at 3756 /note=SSC: 3756-3514 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_STRAWBERRYJAMM_13 [Microbacterium phage StrawberryJamm] ],,NCBI, q1:s1 100.0% 7.2505E-48 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.945, -5.114865311845495, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_STRAWBERRYJAMM_13 [Microbacterium phage StrawberryJamm] ],,QWY80096,100.0,7.2505E-48 SIF-HHPRED: SIF-Syn: Homologues in the neighborhood had no known function. /note=Start 3756 for FrankDeliGuy gene 10 is the most supported. It`s supported by both glimmer and genemark, having coding potential present. The starterator results have this start highly conserved within its genome, and also having the most manual annotations. NKF here as determined by Blast, HHPred, SYN and Mem. CDS complement (3753 - 3992) /gene="10" /product="gp10" /function="hypothetical protein" /locus tag="FrankDeliGuy_10" /note=Original Glimmer call @bp 3992 has strength 6.48; Genemark calls start at 3992 /note=SSC: 3992-3753 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_STRAWBERRYJAMM_14 [Microbacterium phage StrawberryJamm] ],,NCBI, q1:s1 100.0% 7.58922E-49 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.421, -4.301170562178417, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_STRAWBERRYJAMM_14 [Microbacterium phage StrawberryJamm] ],,QWY80097,100.0,7.58922E-49 SIF-HHPRED: SIF-Syn: NKF- same pham as Gazebo_10 and StrawberryJamm_14 /note=Start at 3992 (reverse) is supported by both glimmer and genemark, starterator. It also has LO and has an overlap gap of 4bp. it has multiple 1:1 alignments and has mutliple hts on blast too. There was no result on HHpred and no domain found but it has the same pham as Gazebo_10 and StrawberryJamm_ 14. The function is NKF. CDS complement (3989 - 4345) /gene="11" /product="gp11" /function="helix-turn-helix DNA binding domain" /locus tag="FrankDeliGuy_11" /note=Original Glimmer call @bp 4345 has strength 5.3; Genemark calls start at 4345 /note=SSC: 4345-3989 CP: yes SCS: both ST: SS BLAST-Start: [helix-turn-helix DNA binding domain protein [Microbacterium phage StrawberryJamm]],,NCBI, q1:s1 100.0% 1.83262E-79 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.755, -5.220380545373514, no F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding domain protein [Microbacterium phage StrawberryJamm]],,QWY80098,99.1525,1.83262E-79 SIF-HHPRED: a.4.5.28 (A:8-144) Transcriptional regulator PA3341 {Pseudomonas aeruginosa [TaxId: 287]},,,d2fbha1,75.4237,98.6 SIF-Syn: helix-turn-helix-DNA binding domain protein - same pham as Gazebo_15 and Casend_14 /note=Start at 4345 (reverse) is supported by both glimmer and genemark, starterator and has a coding potential. It has LO and an overlap gap of 8bp and the best RBS. It has multiple 1:1 alignmenst and blast hits. The function found for this gene would Helix-turn-helix DNA binding domain protein. CDS complement (4338 - 4553) /gene="12" /product="gp12" /function="helix-turn-helix DNA binding domain" /locus tag="FrankDeliGuy_12" /note=Original Glimmer call @bp 4553 has strength 6.6; Genemark calls start at 4553 /note=SSC: 4553-4338 CP: yes SCS: both ST: SS BLAST-Start: [helix-turn-helix DNA binding protein [Microbacterium phage Squash] ],,NCBI, q1:s1 100.0% 5.73451E-43 GAP: 61 bp gap LO: no RBS: Kibler 6, Karlin Medium, 0.835, -7.134752732491032, no F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding protein [Microbacterium phage Squash] ],,YP_009801755,100.0,5.73451E-43 SIF-HHPRED: Recombination Directionality Factor RdfS; Excisionase, Recombination Directionality Factor, winged helix-turn-helix, superhelix, DNA BINDING PROTEIN; HET: GOL; 2.45A {Mesorhizobium japonicum R7A},,,8DGL_A,85.9155,98.2 SIF-Syn: helix-turn-helix DNA binding domain protein, same pham as BabyDotz_13 and Casend_15 /note=Start at 4553 (reverse) is supported by both glimmer, genemark, starterator and has a coding potential. However, It doesn`t have Lo and has a long gap of 61. It has a multiple 1:1 alignment of and has hits on blast, HHPred and SIF-SYN. CDS 4615 - 4770 /gene="13" /product="gp13" /function="ribbon-helix-helix DNA binding domain" /locus tag="FrankDeliGuy_13" /note=Original Glimmer call @bp 4615 has strength 10.56; Genemark calls start at 4615 /note=SSC: 4615-4770 CP: yes SCS: both ST: SS BLAST-Start: [DNA binding domain protein [Microbacterium phage Hyperion] ],,NCBI, q1:s1 100.0% 6.12259E-28 GAP: 61 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.994, -5.966653499439317, no F: ribbon-helix-helix DNA binding domain SIF-BLAST: ,,[DNA binding domain protein [Microbacterium phage Hyperion] ],,YP_009801555,100.0,6.12259E-28 SIF-HHPRED: Putative; Helicobacter pylori, repressor, transcriptional regulator, DNA-binding, ribbon-helix-helix, HP0564, JHP0511, UNKNOWN FUNCTION, GENE REGULATION; NMR {Helicobacter pylori},,,2K1O_B,78.4314,98.3 SIF-Syn: ribbon-helix-helix DNA binding domain protein, same pham as AluminumJesus_13 and BabyDotz_14 /note=The start at 4615 is supported by Genemark, Glimmer, starterator, and coding potential. It has multiple 1:1 alignments. It also has the LORF. This protein is likely to be a ribbon helix helix dna binding domain protein, and has synteny with this kind of protein in other members of the cluster. CDS 4767 - 5525 /gene="14" /product="gp14" /function="helix-turn-helix DNA binding domain" /locus tag="FrankDeliGuy_14" /note=Original Glimmer call @bp 4767 has strength 17.9; Genemark calls start at 4767 /note=SSC: 4767-5525 CP: yes SCS: both ST: SS BLAST-Start: [helix-turn-helix DNA-binding domain protein [Microbacterium phage Phabia] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.575, -3.5925599723783583, no F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA-binding domain protein [Microbacterium phage Phabia] ],,QQO39525,100.0,0.0 SIF-HHPRED: a.4.1.2 (C:) HIN recombinase (DNA-binding domain) {Synthetic},,,d1ijwc_,16.2698,95.4 SIF-Syn: helix-turn-helix DNA binding domain protein, same pham as Grassboy_17 and Phabia_16 /note=Start at 4615 is supported by genemark and glimmer, starterator and has a coding potential. However, it doesn`t have LO and has the longest gap of 61. It has 1:1 multiple alignments and blast hits . Also has the same pham as AliminumJesus_13 and BabyDotz_14, and the function is Ribbon-helix-helix-DNA binding domain protein. CDS 5685 - 5858 /gene="15" /product="gp15" /function="hypothetical protein" /locus tag="FrankDeliGuy_15" /note=Original Glimmer call @bp 5685 has strength 5.41; Genemark calls start at 5685 /note=SSC: 5685-5858 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_RUDY_16 [Microbacterium phage Rudy] ],,NCBI, q1:s1 100.0% 3.7191E-33 GAP: 159 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.411, -6.526515810972826, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_RUDY_16 [Microbacterium phage Rudy] ],,QQO39198,100.0,3.7191E-33 SIF-HHPRED: SIF-Syn: NKF, Same Pham, Position, and Gene Neighborhood as Babydotz_17 and Blab_15 /note=Start at 5685 is supported by both Glimmer and GeneMark, showing high conservation within the genome. It has multiple 1:1 alignments, which further strengthens its validity. The gene contains the LORF and has a favorable RBS score of 1.411 with a spacing of -6. The substantial evidence backing this start site outweighs the gaps present. The function of this protein is annotated as "NKF." This conclusion is based on SIF-BLAST, which returned no alignments with a known function, and SIF-HHPred, which provided no hits with >90% probability. Additionally, synteny analysis indicates that this gene shares the same pham and neighborhood as BabyDotz_17, Blab_15, and RUDY_16, all of which also lack known functions. No transmembrane regions were predicted by TMHMM, supporting the NKF assignment. CDS 5858 - 6232 /gene="16" /product="gp16" /function="ribbon-helix-helix DNA binding domain" /locus tag="FrankDeliGuy_16" /note=Original Glimmer call @bp 5858 has strength 8.81; Genemark calls start at 5858 /note=SSC: 5858-6232 CP: yes SCS: both ST: SS BLAST-Start: [DNA binding protein [Microbacterium phage Squash] ],,NCBI, q1:s1 100.0% 2.15916E-82 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.14, -5.009000456184513, no F: ribbon-helix-helix DNA binding domain SIF-BLAST: ,,[DNA binding protein [Microbacterium phage Squash] ],,YP_009801760,100.0,2.15916E-82 SIF-HHPRED: Putative; Helicobacter pylori, repressor, transcriptional regulator, DNA-binding, ribbon-helix-helix, HP0564, JHP0511, UNKNOWN FUNCTION, GENE REGULATION; NMR {Helicobacter pylori},,,2K1O_B,34.6774,95.6 SIF-Syn: DNA binding protein, same pham and neighbourhood as Squash_21, Namago_19, Nike_19 /note=Start at 5858 is supported by both Glimmer and GeneMark, demonstrating significant conservation within the genome. It has multiple 1:1 alignments that reinforce its validity. The gene does not contain a LORF, but it has a favorable RBS score of 2.14 with a spacing of -5.009. Despite the upstream gap of -1 base pair, the substantial evidence for this start site is compelling. The function of this protein is annotated as "DNA binding protein." Although SIF-BLAST returned no hits with a low e-value (<10^-4), synteny analysis shows that this gene shares the same pham and neighborhood as Squash_21, Namago_19, and Nike_19. No transmembrane regions were predicted by TMHMM. This gene had a grouping of beta-sheets following by a short spacer than a group of alpha-helices, indicated that it is a ribbon-helix-helix DNA binding domain protein. CDS 6207 - 7718 /gene="17" /product="gp17" /function="terminase" /locus tag="FrankDeliGuy_17" /note=Original Glimmer call @bp 6207 has strength 11.25; Genemark calls start at 6207 /note=SSC: 6207-7718 CP: yes SCS: both ST: SS BLAST-Start: [terminase large subunit [Microbacterium phage Hyperion] ],,NCBI, q1:s1 100.0% 0.0 GAP: -26 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.323, -4.038963679561956, no F: terminase SIF-BLAST: ,,[terminase large subunit [Microbacterium phage Hyperion] ],,YP_009801560,100.0,0.0 SIF-HHPRED: Large subunit terminase; large terminase, VIRAL PROTEIN; 2.2A {Deep-sea thermophilic phage D6E},,,5OE8_A,78.7276,99.9 SIF-Syn: terminase large subunit, same pham and neighbourhood as Hyperion_ 18, BabyDotz _19, Blab_17 /note=Start at 6207 is supported by both Glimmer and GeneMark, indicating a strong consensus for this start site. It has multiple 1:1 alignments, confirming its presence within the genome. This gene does not contain a LORF, but it features a robust RBS score of 2.323 with a spacing of -4.039. The upstream gap of -26 base pairs is notable; however, the overall evidence strongly supports this start site. The function of this protein is annotated as "terminase large subunit." This conclusion is bolstered by SIF-BLAST, which identified a significant alignment with the large subunit terminase from Deep-sea thermophilic phage D6E, with a high probability of 99.9% and a coverage of 78.7%. The gene shares the same pham and neighborhood as Hyperion_18, BabyDotz_19, and Blab_17, indicating functional relatedness. No transmembrane regions were predicted by TMHMM. Since there is only one terminase unit the function will be terminase rather than "terminase, large subunit" CDS 7715 - 8053 /gene="18" /product="gp18" /function="membrane protein" /locus tag="FrankDeliGuy_18" /note=Original Glimmer call @bp 7715 has strength 9.06; Genemark calls start at 7715 /note=SSC: 7715-8053 CP: yes SCS: both ST: SS BLAST-Start: [membrane protein [Microbacterium phage Zagie] ],,NCBI, q1:s1 100.0% 1.06485E-71 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.794, -2.996985748252251, yes F: membrane protein SIF-BLAST: ,,[membrane protein [Microbacterium phage Zagie] ],,UVG35374,100.0,1.06485E-71 SIF-HHPRED: DUF1360 ; Protein of unknown function (DUF1360),,,PF07098.14,80.3571,99.6 SIF-Syn: membrane protein, same pham and neighbourhood as Zagie_ 21, Rowlf_17, BabyDotz_20 /note=Start at 7715 is supported by both Glimmer and GeneMark, establishing a strong foundation for this start site. The gene has a 1:1 alignment with Microbacterium phage Zagie_21 in NCBI, with 100% coverage and an E-value of 1.06485e-71, further confirming its relevance within the genome. It contains a LORF and features a high RBS score of 2.794 with a spacing of -2.997, suggesting a strong ribosomal binding site. This start site is optimal given its alignment and RBS score. The function of this protein is annotated as "membrane protein," based on SIF-BLAST, which identified a match with the membrane protein from Microbacterium phage Zagie_21, showing 100% coverage and a highly significant E-value. Additionally, Pfam identifies this protein under the "protein of unknown function" (DUF1360) family, with PF07098.14 covering 80.4% and showing a high probability of 99.6%. The gene shares the same pham and neighborhood as Zagie_21, Rowlf_17, and BabyDotz_20, indicating functional relatedness. TMHMM predicts three transmembrane domain, which further supports its classification as a membrane protein. CDS 8306 - 10132 /gene="19" /product="gp19" /function="portal protein" /locus tag="FrankDeliGuy_19" /note=Original Glimmer call @bp 8306 has strength 12.25; Genemark calls start at 8306 /note=SSC: 8306-10132 CP: yes SCS: both ST: SS BLAST-Start: [portal protein [Microbacterium phage Zagie] ],,NCBI, q1:s1 100.0% 0.0 GAP: 252 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.736, -3.196631460098011, no F: portal protein SIF-BLAST: ,,[portal protein [Microbacterium phage Zagie] ],,UVG35375,100.0,0.0 SIF-HHPRED: Portal protein; Prohead I, icosahedral symmetry, HK97, phage, capsid, VIRUS; 3.6A {Escherichia phage HK97},,,8FQL_D,62.0066,99.9 SIF-Syn: Portal Protein, Same Pham, Position, and Gene Neighborhood as Rowlf_18 and SallyK_23 /note=Start at 8306 is supported by both Glimmer and GeneMark, establishing a strong foundation for this start site. Starterator supports this start . The gene has multiple 1:1 alignments, confirming its relevance within the genome. It contains a LORF and features a high RBS score of 2.736 with a spacing of -3.197. Although there is a significant upstream gap of 252 base pairs, the substantial evidence supports the selection of this start site. The function of this protein is annotated as "portal protein," based on SIF-BLAST, which identified a match with the portal protein from Microbacterium phage Zagie_22, showing 100% coverage and a high probability of 99.9%. Additionally, it aligns with the portal protein from Escherichia phage HK97, providing further functional context. The gene shares the same pham and neighborhood as Zagie_22, Rowlf_18, and SallyK_23, indicating a strong functional relatedness. No transmembrane regions were predicted by TMHMM, further supporting the classification as a portal protein. CDS 10132 - 13446 /gene="20" /product="gp20" /function="capsid maturation protease" /locus tag="FrankDeliGuy_20" /note=Original Glimmer call @bp 10132 has strength 10.03; Genemark calls start at 10132 /note=SSC: 10132-13446 CP: yes SCS: both ST: SS BLAST-Start: [virion structural protein [Microbacterium phage Hyperion] ],,NCBI, q1:s1 100.0% 0.0 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.021, -4.957272661945414, no F: capsid maturation protease SIF-BLAST: ,,[virion structural protein [Microbacterium phage Hyperion] ],,YP_009801564,98.7319,0.0 SIF-HHPRED: SIF-Syn: capsid maturation protease and MuF-like fusion protein, same pham and same position as Mashley_21 and Namago_23, all are immediately downstream from portal protein and upstream of major capsid hexamer protein /note=SIF-Blast: capsid maturation protease and MuF-like fusion protein, Microbacterium phage Mashley, NCBI, QED11838, 100%, E-val 0 /note= /note=SIF-HHPred: NKF, no significant results CDS 13450 - 13632 /gene="21" /product="gp21" /function="hypothetical protein" /locus tag="FrankDeliGuy_21" /note=Original Glimmer call @bp 13483 has strength 10.91; Genemark calls start at 13483 /note=SSC: 13450-13632 CP: yes SCS: both-cs ST: SS BLAST-Start: [hypothetical protein SEA_ALUMINUMJESUS_22 [Microbacterium phage AluminumJesus]],,NCBI, q1:s1 100.0% 3.10589E-34 GAP: 3 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.559, -3.83711508627892, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ALUMINUMJESUS_22 [Microbacterium phage AluminumJesus]],,UJD20758,98.3333,3.10589E-34 SIF-HHPRED: SIF-Syn: NKF - homologs have no known function /note=SIF-Blast: NKF: hypothetical protein /note= /note=SIF-HHPred: NKF: hypothetical protein CDS 13770 - 15602 /gene="22" /product="gp22" /function="major capsid hexamer protein" /locus tag="FrankDeliGuy_22" /note=Original Glimmer call @bp 13770 has strength 13.29; Genemark calls start at 13770 /note=SSC: 13770-15602 CP: yes SCS: both ST: SS BLAST-Start: [major capsid hexamer protein [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 0.0 GAP: 137 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.06, -2.5052746077145835, yes F: major capsid hexamer protein SIF-BLAST: ,,[major capsid hexamer protein [Microbacterium phage Judebell]],,XCG97241,99.3443,0.0 SIF-HHPRED: SIF-Syn: major capsid hexamer protein, same pham and same position as Judebell_27 and Casend_26, all are downstream from capsid maturation protease and upstream of major tail protein /note=SIF-Blast: major capsid hexamer protein, Microbacterium phage Judebell, NCBI, XCG97241, 100%, Eval 0 /note= /note=SIF-HHPred: NKF, no significant results CDS 15667 - 16401 /gene="23" /product="gp23" /function="hypothetical protein" /locus tag="FrankDeliGuy_23" /note=Original Glimmer call @bp 15667 has strength 11.55; Genemark calls start at 15667 /note=SSC: 15667-16401 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_NIKE_26 [Microbacterium phage Nike] ],,NCBI, q1:s1 100.0% 4.67708E-174 GAP: 64 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.47, -3.670670413150579, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_NIKE_26 [Microbacterium phage Nike] ],,QIQ63610,100.0,4.67708E-174 SIF-HHPRED: SIF-Syn: NKF - homologs have no known function /note=SIF-Blast: hypothetical protein, Microbacterium phage Nike, NCBI, QIQ63610, 100%, eval 4.67708e-174 /note= /note=SIF-HHPred: NKF, no significant results CDS 16411 - 16977 /gene="24" /product="gp24" /function="hypothetical protein" /locus tag="FrankDeliGuy_24" /note=Original Glimmer call @bp 16411 has strength 13.41; Genemark calls start at 16411 /note=SSC: 16411-16977 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CASEND_28 [Microbacterium phage Casend] ],,NCBI, q1:s1 100.0% 1.64831E-123 GAP: 9 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.306, -2.0111200136961407, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_28 [Microbacterium phage Casend] ],,QNL29995,100.0,1.64831E-123 SIF-HHPRED: SIF-Syn: NKF - homologs have no known function /note=SIF-Blast: NKF, no alignments had known functions /note= /note=SIF-HHPred: NKF - no significant results, low coverage, e values above 8.1+ CDS 17008 - 17955 /gene="25" /product="gp25" /function="major tail protein" /locus tag="FrankDeliGuy_25" /note=Original Glimmer call @bp 17032 has strength 12.62; Genemark calls start at 17032 /note=SSC: 17008-17955 CP: yes SCS: both-cs ST: SS BLAST-Start: [major tail protein [Microbacterium phage Casend] ],,NCBI, q1:s1 100.0% 0.0 GAP: 30 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.39, -3.8366550365551095, yes F: major tail protein SIF-BLAST: ,,[major tail protein [Microbacterium phage Casend] ],,QNL29996,100.0,0.0 SIF-HHPRED: b.1.3.1 (A:) VPS10 domain-containing receptor SorCS2 {Human (Homo sapiens) [TaxId: 9606]},,,d1wgoa_,19.3651,96.7 SIF-Syn: NKF - homologs have no known function not similar positions nor pham (except for frankdeliguy_26 and rudy_26 same pham) /note=start is supported by both . for blast-start, this is a major tail protein with 1:1 ratio and high percentage and very low e-val close to 0. this does not have a LO and it has the best RBS. for functions: BLAST is showing very low e-val but it is also showing major tail protein and when you move onto HHPred you see thatthe coverage and probability is inadequate with the amount that`s required and the e-val is not close to 0. SIF-Syn shows us that there is really NKF and that positions and phams do not match at all. so among all this evidence , we can conclude that this gene has no known functions but since there is sufficient evidence that can be overridden with, we can conclude that this is a major tail capsid protein as supported by both CDS 18079 - 18960 /gene="26" /product="gp26" /function="hypothetical protein" /locus tag="FrankDeliGuy_26" /note=Original Glimmer call @bp 18079 has strength 13.05; Genemark calls start at 18079 /note=SSC: 18079-18960 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 0.0 GAP: 123 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.905, -3.59360038608048, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein [Microbacterium phage Judebell]],,XCG97245,100.0,0.0 SIF-HHPRED: SIF-Syn: NKF - homologs have no known function not similar positions nor phams /note=start is supported by both. for blast-start, this is a hypothetical protein with 1:1 ratio and high percentage and very low e-val close to 0. this does have a LO and it has the best RBS. for functions: BLAST is showing very low e-val but it is also showing hypothetical protein and when you move onto HHPred you see that the coverage and probability is inadequate with the amount that`s required and the e-val is not close to 0. SIF-Syn shows us that there is really NKF and that positions and phams do not match at all. so among all this evidence , we can conclude that this gene has no known functions CDS 18963 - 20501 /gene="27" /product="gp27" /function="phosphoesterase" /locus tag="FrankDeliGuy_27" /note=Original Glimmer call @bp 18963 has strength 11.11; Genemark calls start at 18963 /note=SSC: 18963-20501 CP: yes SCS: both ST: SS BLAST-Start: [phosphoesterase [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 0.0 GAP: 2 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.813, -5.69021729366323, no F: phosphoesterase SIF-BLAST: ,,[phosphoesterase [Microbacterium phage Judebell]],,XCG97246,100.0,0.0 SIF-HHPRED: c.1.18.3 (A:1-240) Glycerophosphodiester phosphodiesterase UgpQ {Agrobacterium tumefaciens [TaxId: 358]},,,d1zcca1,46.2891,100.0 SIF-Syn: NKF - homologs have no known function not similar positions nor pham /note=start is supported by both. for blast-start, this is a phosphoesterase with 1:1 ratio and high percentage and very low e-val close to 0. this does not have a LO and it doesn`t have the best RBS. for functions: BLAST is shwing very low e-val but it is also showing phosphoesterase and when you move onto HHPred you see thatthe coverage and probability is inadequate with the amount that`s required and the e-val is not close to 0. SIF-Syn shows us that there is really NKF and that positions and phams do not match at all. so among all this evidence , we can conclude that this gene has a function of phosphoesterase CDS 20533 - 21381 /gene="28" /product="gp28" /function="head-to-tail adaptor" /locus tag="FrankDeliGuy_28" /note=Original Glimmer call @bp 20533 has strength 13.44; Genemark calls start at 20533 /note=SSC: 20533-21381 CP: yes SCS: both ST: SS BLAST-Start: [head-to-tail adaptor [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 0.0 GAP: 31 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.14, -2.627458653341447, yes F: head-to-tail adaptor SIF-BLAST: ,,[head-to-tail adaptor [Microbacterium phage Judebell]],,XCG97247,100.0,0.0 SIF-HHPRED: Head completion protein; Neck, Portal, T5, VIRUS, VIRAL PROTEIN; 3.2A {Escherichia phage DT57C},,,8HQO_R,80.8511,98.5 SIF-Syn: NKF - homologs have no known function not similar positions nor pham /note=start is supported by both . for blast-start, this is a head-to-tail adaptor with 1:1 ratio and high percentage and very low e-val close to 0. this does have a LO and it has the best RBS. for functions: BLAST is shwing very low e-val but it is also showing head-to-tail adaptor and when you move onto HHPred you see thatthe coverage and probability is inadequate with the amount that`s required and the e-val is not close to 0. SIF-Syn shows us that there is really NKF and that positions and phams do not match at all. so among all this evidence , we can conclude that this gene has a head-to-tail adaptor function CDS 21381 - 21908 /gene="29" /product="gp29" /function="hypothetical protein" /locus tag="FrankDeliGuy_29" /note=Original Glimmer call @bp 21381 has strength 11.26; Genemark calls start at 21381 /note=SSC: 21381-21908 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 2.84664E-123 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.601, -5.540690543217318, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein [Microbacterium phage Judebell]],,XCG97248,100.0,2.84664E-123 SIF-HHPRED: SIF-Syn: NKF - homologs have no known function not similar positions nor phams /note=start is supported by both. for blast-start, this is a hypothetical protein with 1:1 ratio and high percentage and very low e-val close to 0. this does have a LO and it doesn`t have the best RBS. for functions: BLAST is shwing very low e-val but it is also showing hypothetical protein and when you move onto HHPred you see thatthe coverage and probability is inadequate with the amount that`s required and the e-val is not close to 0. SIF-Syn shows us that there is really NKF and that positions and phams do not match at all. so among all this evidence , we can conclude that this gene has no known functions CDS 21910 - 22314 /gene="30" /product="gp30" /function="hypothetical protein" /locus tag="FrankDeliGuy_30" /note=Original Glimmer call @bp 21910 has strength 8.73; Genemark calls start at 21910 /note=SSC: 21910-22314 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 3.43277E-90 GAP: 1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.603, -4.699110570850454, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein [Microbacterium phage Judebell]],,XCG97249,100.0,3.43277E-90 SIF-HHPRED: Minor_capsid_2 ; Minor capsid protein,,,PF11114.11,97.0149,98.9 SIF-Syn: NKF - in the same pham and position of Casend_ 34 and Judebell_35 both of which are NKF /note=Starterator, glimmer, and genemark all call the same start, good gap score and best rbs scores, 1:1 alignment, HHPred showed high probability and coverage, starterator calls the most annotated start which has 38 MA`s, no known function - phamerator showed no neighboring genes in same position or pham with a function, longest open reading frame CDS 22351 - 22809 /gene="31" /product="gp31" /function="tail assembly chaperone" /locus tag="FrankDeliGuy_31" /note=Original Glimmer call @bp 22351 has strength 8.97; Genemark calls start at 22351 /note=SSC: 22351-22809 CP: yes SCS: both ST: SS BLAST-Start: [tail assembly chaperone [Microbacterium phage Namago] ],,NCBI, q1:s1 100.0% 3.17564E-105 GAP: 36 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.758, -3.133663537764895, yes F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Microbacterium phage Namago] ],,QQO39435,100.0,3.17564E-105 SIF-HHPRED: Phage_TAC_10 ; Phage tail assembly chaperone,,,PF10963.11,57.8947,94.9 SIF-Syn: tail assembly chaperone, same pham and similar position as Namago_34 and Grassboy_35, downstream of head-to-tail adaptors and upstream of tape measure proteins. /note=Start is supported by Glimmer, GeneMark, and Starterator, and includes all coding potential. Also has the top hits that align 1:1. Does not have -4 overlap and has 36 bp gap, is not the longest ORF, but other evidence suggests this as start. Best RBS. Function (tail assembly chaperone) supported by BLAST and Phamerator maps. CDS join(22351..22770,22770..23003) /gene="32" /product="gp32" /function="tail assembly chaperone" /locus tag="FrankDeliGuy_32" /note= /note=SSC: 22351-23003 CP: yes SCS: neither ST: SS BLAST-Start: [tail assembly chaperone [Microbacterium phage Namago]],,NCBI, q1:s1 100.0% 5.66898E-156 GAP: -459 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.758, -3.133663537764895, yes F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Microbacterium phage Namago]],,QQO39436,100.0,5.66898E-156 SIF-HHPRED: SIF-Syn: tail assembly chaperone, same pham and similar position as Namago_34 and Grassboy_35, downstream of head-to-tail adaptors and upstream of tape measure proteins. /note=Start is supported by Glimmer, GeneMark, and Starterator, and includes all coding potential. Also has the top hits that align 1:1. Does not have -4 overlap and has 36 bp gap, is not the longest ORF, but other evidence suggests this as start. Best RBS. Function (tail assembly chaperone) supported by BLAST and Phamerator maps. CDS 23012 - 26188 /gene="33" /product="gp33" /function="tape measure protein" /locus tag="FrankDeliGuy_33" /note=Original Glimmer call @bp 23012 has strength 10.11; Genemark calls start at 23012 /note=SSC: 23012-26188 CP: yes SCS: both ST: SS BLAST-Start: [tape measure protein [Microbacterium phage Namago] ],,NCBI, q1:s1 100.0% 0.0 GAP: 7 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.155, -4.4057176022952405, no F: tape measure protein SIF-BLAST: ,,[tape measure protein [Microbacterium phage Namago] ],,QQO39437,99.9055,0.0 SIF-HHPRED: SIF-Syn: tape measure protein, same pham and position as Wheelie_35 and Lonelysoil_35, all downstream from tail assembly chaperone and upstream from minor tail protein. /note=Start is supported by Glimmer, GeneMark, and Starterator, and includes all coding potential. Has top hits that align 1:1 with good coverage and e-value. Has an 8 bp gap, which is the smallest out of other alternatives. Longest ORF. Best RBS for considered candidates. Function is supported by BLAST and Phamerator maps. CDS 26197 - 27537 /gene="34" /product="gp34" /function="minor tail protein" /locus tag="FrankDeliGuy_34" /note=Original Glimmer call @bp 26197 has strength 4.95; Genemark calls start at 26197 /note=SSC: 26197-27537 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Microbacterium phage Casend] ],,NCBI, q1:s1 100.0% 0.0 GAP: 8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.155, -4.4057176022952405, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage Casend] ],,QNL30005,99.7758,0.0 SIF-HHPRED: SIF-Syn: minor tail protein, same pham and similar position as Lonelysoil_36 and Casend_36, downstream from tape measure protein and upstream from minor tail protein /note=Start is supported by Glimmer, GeneMark, and Starterator. Also includes all coding potential. Has the top hits that align 1:1 with good coverage and e-value. Has the smallest gap out of other alternatives. Also has the longest ORF and best RBS. Function is supported by BLAST and Phamerator maps. CDS 27557 - 30484 /gene="35" /product="gp35" /function="minor tail protein" /locus tag="FrankDeliGuy_35" /note=Original Glimmer call @bp 27557 has strength 7.57; Genemark calls start at 27557 /note=SSC: 27557-30484 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 0.0 GAP: 19 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.645, -3.4470622626190464, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage Judebell]],,XCG97254,99.8974,0.0 SIF-HHPRED: Interleukin-12 receptor subunit beta-2,Calmodulin-1; Complex, Cytokine, Receptor, SIGNALING PROTEIN; HET: BMA, NAG, MAN;{Mus musculus},,,8ODZ_D,50.7692,99.6 SIF-Syn: Minor tail protein, Same pham and similar position as Casend_39 and Judebell_40, upstream and downstream of minor tail proteins. /note=This start is the most annotated start candidate, it is called by both Glimmer and Genemark, it has the longest open reading frame, and the best RBS. The function of this gene appears to be a minor tail protein as suggested by both Synteny and BLAST. HHPred suggests otherwise, but with low coverage and a function that is not found on the official phage function list. CDS 30484 - 31629 /gene="36" /product="gp36" /function="minor tail protein" /locus tag="FrankDeliGuy_36" /note=Original Glimmer call @bp 30484 has strength 10.5; Genemark calls start at 30484 /note=SSC: 30484-31629 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 0.0 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.849, -2.881875840424956, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage Judebell]],,XCG97255,99.7375,0.0 SIF-HHPRED: Sipho_Gp37 ; Siphovirus ReqiPepy6 Gp37-like protein,,,PF14594.9,92.126,99.7 SIF-Syn: Minor tail protein, same pham and similar position as Zagie_38 and Judebell_41, upstream of minor tail protein and downstream of NKF. /note=This start is the most annotated start candidate, it is called by both Glimmer and Genemark, and possesses the best RBS. The function of this gene appears to be a minor tail protein as suggested by both Synteny and BLAST, and though HHPred suggests a different protein the one it gives is not an approved function. CDS 31629 - 32390 /gene="37" /product="gp37" /function="hypothetical protein" /locus tag="FrankDeliGuy_37" /note=Original Glimmer call @bp 31629 has strength 8.49; Genemark calls start at 31629 /note=SSC: 31629-32390 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 2.6964E-179 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.47, -3.8116689268127657, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein [Microbacterium phage Judebell]],,XCG97256,100.0,2.6964E-179 SIF-HHPRED: SIF-Syn: NKF, same pham and similar position as Casend_41 and Judebell_42, immediately upstream of minor tail protein and five genes downstream of endolysin. /note=This start is the most annotated start candidate, it is called by both Glimmer and Genemark, and posseses the best RBS. The function of this gene appears to be unknown, as the significant hits from BLAST and Synteny were only to those of unknown functions, and HHPred produced no significant alignments. CDS 32402 - 32665 /gene="38" /product="gp38" /function="hypothetical protein" /locus tag="FrankDeliGuy_38" /note=Original Glimmer call @bp 32402 has strength 11.19; Genemark calls start at 32402 /note=SSC: 32402-32665 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 1.14323E-54 GAP: 11 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.14, -2.2763497933341483, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein [Microbacterium phage Judebell]],,XCG97257,100.0,1.14323E-54 SIF-HHPRED: SIF-Syn: NKF, same pham and similar position as Casend_42 and Judebell_43, two genes upstream of minor tail protein and four genes downstream of endolysin. /note=This start is the most annotated start candidate, it is called by both Glimmer and Genemark, and possesses the best RBS. The function of this gene appears to be unknown, as the significant hits from BLAST were only to those of unknown functions, and HHPred produced no significant alignments. CDS 32739 - 33203 /gene="39" /product="gp39" /function="membrane protein" /locus tag="FrankDeliGuy_39" /note=Original Glimmer call @bp 32739 has strength 10.03; Genemark calls start at 32739 /note=SSC: 32739-33203 CP: yes SCS: both ST: SS BLAST-Start: [membrane protein [Microbacterium phage Hyperion] ],,NCBI, q1:s1 100.0% 5.03226E-106 GAP: 73 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.151, -2.394485424036831, yes F: membrane protein SIF-BLAST: ,,[membrane protein [Microbacterium phage Hyperion] ],,YP_009801583,100.0,5.03226E-106 SIF-HHPRED: SIF-Syn: NKF, same pham and similar position as Casend_43 and Hyperion_41, three genes upstream of minor tail protein and three genes downstream of endolysin. /note=This start is the most annotated start candidate, it is called by both Glimmer and Genemark, has the longest ORF, and possesses the best RBS. The function of this gene appears to be a membrane protein, as the significant hits from BLAST and transmembrane domain found by DeepTMHMM suggest. /note= /note=One transmembrane domain found (DeepTMHMM) CDS 33193 - 33390 /gene="40" /product="gp40" /function="hypothetical protein" /locus tag="FrankDeliGuy_40" /note=Original Glimmer call @bp 33193 has strength 11.63; Genemark calls start at 33193 /note=SSC: 33193-33390 CP: no SCS: both ST: NI BLAST-Start: [hypothetical protein HOT27_gp042 [Microbacterium phage Hyperion] ],,NCBI, q1:s1 100.0% 4.87859E-39 GAP: -11 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.984, -3.2535385006449706, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein HOT27_gp042 [Microbacterium phage Hyperion] ],,YP_009801584,100.0,4.87859E-39 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function. /note=Start 33193 is supported by Glimmer, Genemark & Starterator. Starterator claims it to be 96.1% conserved, 98% called, and having 34/37 Manual Annotations. It is also the longest open reading frame, has the best RBS scores, and multiple 1:1 Blast results from NCBI with good E-values. There is no known function for this protein. The alignments from BLAST are hypothetical proteins. Hits from HHPred are insignificant as they had large e-values and probabilities lower than 90%. From the phamerator, the homologs had no known function despite having positional similarity. There is no transmembrane domains. CDS 33395 - 34132 /gene="41" /product="gp41" /function="membrane protein" /locus tag="FrankDeliGuy_41" /note=Original Glimmer call @bp 33395 has strength 17.58; Genemark calls start at 33395 /note=SSC: 33395-34132 CP: yes SCS: both ST: SS BLAST-Start: [membrane protein [Microbacterium phage Blab] ],,NCBI, q1:s1 100.0% 1.99127E-173 GAP: 4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.458, -5.8568091738896655, no F: membrane protein SIF-BLAST: ,,[membrane protein [Microbacterium phage Blab] ],,QXN73968,100.0,1.99127E-173 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function. /note=Start 33395 is supported by Glimmer, Genemark & Starterator. It is 90.4% conserved, 85.1% called, and have 29/38 Manual Annotations. It is also the longest open reading frame, has a gap of 4bp and many 1:1 Blast alignments. Not the best RBS scores, but other RBS scores were also unfavorable and this is compensated by other data. Function is membrane protein as Blast results have many 1:1 allignments with membrane protein as an assigned function and TMHMM dectects 5 membrane domains. HHPred has no significant results and homologs had no known function but rest of evidence supports membrane protein. CDS 34202 - 35023 /gene="42" /product="gp42" /function="endolysin" /locus tag="FrankDeliGuy_42" /note=Original Glimmer call @bp 34202 has strength 13.26; Genemark calls start at 34202 /note=SSC: 34202-35023 CP: yes SCS: both ST: SS BLAST-Start: [endolysin [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 0.0 GAP: 69 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.32, -4.045036357814993, no F: endolysin SIF-BLAST: ,,[endolysin [Microbacterium phage Judebell]],,XCG97261,100.0,0.0 SIF-HHPRED: d.3.1.16 (A:87-234) Cell wall-associated hydrolase Spr C-terminal domain {Nostoc punctiforme [TaxId: 272131]},,,d2evra2,30.0366,99.0 SIF-Syn: Endolysin. Same pham, and positional similarity as Blab_43, Judebell_47, and Rowlf_41, all downstream of a pham 52 gene and upstream of a pham 49 gene, /note=Start 34202 is supported by Glimmer, Genemark & Starterator. It is 100% conserved, 100% called, and have 34/34 Manual Annotations. It is the longest open reading frame, has the best RBS scores and many 1:1 Blast allignments. Gap of 69bp is longer, but it is the shortest possible gap for this gene. The assigned function is Endolysin, has many 1:1 Blast results with good e-values for Endolysins. HHPred has no significant results for protein functions relevant for a phage, significant results all have coverage of about 30% or less, which lowers the weight of this evidence significantly compared to blast. Supported by SIF-Syn. No membrane domains found. CDS complement (35093 - 35605) /gene="43" /product="gp43" /function="hypothetical protein" /locus tag="FrankDeliGuy_43" /note=Original Glimmer call @bp 35605 has strength 10.58; Genemark calls start at 35512 /note=SSC: 35605-35093 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein SEA_BLAB_44 [Microbacterium phage Blab]],,NCBI, q1:s1 100.0% 1.51874E-115 GAP: -32 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.905, -3.1164791313608173, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_BLAB_44 [Microbacterium phage Blab]],,QXN73970,98.8235,1.51874E-115 SIF-HHPRED: SIF-Syn: NFK, homologs have no known function /note=Start 35605 is supported by Glimmer & Starterator, but not by Genemark. It is 100% conserved, 85.7% called, and have 35/38 Manual Annotations. There are many 1:1 Blast results and the RBS scores are the best. This is not the longest open reading frame, and there is a 32bp overlap, which is large, but other evidence strongly supports the chosen start, this is the shortest overlap, and the shortest gap. No function has been assigned, all significant Blast results have no known function, and the few significant HHPred results have extremley low coverage and functions unrelated to phages. no homologs have known functions. No membrane domains detected by TMHMM. CDS complement (35574 - 35876) /gene="44" /product="gp44" /function="hypothetical protein" /locus tag="FrankDeliGuy_44" /note=Original Glimmer call @bp 35876 has strength 4.81; Genemark calls start at 35876 /note=SSC: 35876-35574 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CASEND_48 [Microbacterium phage Casend] ],,NCBI, q1:s1 100.0% 1.19903E-63 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.188, -4.336903751127196, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_48 [Microbacterium phage Casend] ],,QNL30015,100.0,1.19903E-63 SIF-HHPRED: SIF-Syn: NFK, homologs have no known function /note=Start 35876 is the only option presented in PECAAN, it is supported by Glimmer, GeneMark, & Starterator. It is 82.7% conserved, 93% called, and have 29/38 Manual Annotations. The -4 bp gap strongly supports this start, and it has many 1:1 blast results. Does have longest open reading frame and best RBS but this is not the strongest information as there are no other starts to compare to, but RBS scores are considered good. No function has been assigned, all the significant blast results have no known function, no significant HHpred results, no homologs have known functions, and TMHMM has results in no membrane domains. CDS complement (35873 - 36535) /gene="45" /product="gp45" /function="chaperonin, DnaJ-like" /locus tag="FrankDeliGuy_45" /note=Original Glimmer call @bp 36535 has strength 11.72; Genemark calls start at 36535 /note=SSC: 36535-35873 CP: yes SCS: both ST: SS BLAST-Start: [DnaJ-like chaperonin [Microbacterium phage Rowlf]],,NCBI, q1:s1 100.0% 5.12004E-152 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.091, -4.522979412939132, no F: chaperonin, DnaJ-like SIF-BLAST: ,,[DnaJ-like chaperonin [Microbacterium phage Rowlf]],,XEN17627,98.6364,5.12004E-152 SIF-HHPRED: SIF-Syn: DnaJ-like chaperonin, same pham and position as Rowlf_44; is immediately downstream of endolysin and upstream of exonuclease. /note=The Start is supported by both Glimmer and GeneMark. Coding potential is present. There is an overlap of 4 bps, and it has the logest open reading frame. It also has a strong Z score (2.091), and has one of the 2 best RBS HHPred shows NKF as no significant results. SYN shows how many of the positions and phams are not the same. Many 1:1 blast alignments. The top BLAST hits were all DnaJ-like chaperonins. CDS complement (36532 - 36873) /gene="46" /product="gp46" /function="hypothetical protein" /locus tag="FrankDeliGuy_46" /note=Original Glimmer call @bp 36873 has strength 11.85; Genemark calls start at 36873 /note=SSC: 36873-36532 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_NAMAGO_49 [Microbacterium phage Namago] ],,NCBI, q1:s1 100.0% 3.78155E-76 GAP: 21 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.06, -2.442961286954254, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_NAMAGO_49 [Microbacterium phage Namago] ],,QQO39450,100.0,3.78155E-76 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function. /note=This start was called by both GeneMark and Glimmer. There is coding potential and has the best RBS. This start provides the longest open reading frame and has the best overall score of -2.443. Top BLASTT hits were all hypothetical proteins. Homologs had no known functions. Hits from HHPred also had large e-values and probabilities lower than 90%. CDS complement (36895 - 39162) /gene="47" /product="gp47" /function="Cas4 exonuclease" /locus tag="FrankDeliGuy_47" /note=Original Glimmer call @bp 39162 has strength 15.1; Genemark calls start at 39162 /note=SSC: 39162-36895 CP: yes SCS: both ST: SS BLAST-Start: [Cas4 family exonuclease [Microbacterium phage Namago]],,NCBI, q1:s1 100.0% 0.0 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.575, -3.513874779476501, no F: Cas4 exonuclease SIF-BLAST: ,,[Cas4 family exonuclease [Microbacterium phage Namago]],,QQO39451,96.0383,0.0 SIF-HHPRED: Exonuclease V; HYDROLASE; HET: EDO; 2.5A {Homo sapiens},,,7LW7_A,33.7748,98.3 SIF-Syn: Cas4 family exonuclease, same pham, and positional similarity as Phabia_50, Quammi_48, and Rudy_48, all downstream of a pham 25 gene and upstream of a pham 52 gene. /note=Both glimmer and genemark called it as a gene, agreed on the start. There is coding potential. Is not the longest open reading frame, but best RBS. Many 1:1 alignments from blast results, and 1bp overlap. The gene is a Cas4 family exonuclease. SIF-BLAST shows top hits that encode for Cas4 family exonucleases. This is also Supported by SIF-Syn. CDS complement (39162 - 40175) /gene="48" /product="gp48" /function="hypothetical protein" /locus tag="FrankDeliGuy_48" /note=Original Glimmer call @bp 40175 has strength 16.49; Genemark calls start at 40175 /note=SSC: 40175-39162 CP: yes SCS: both ST: SS BLAST-Start: [DNA recombinase [Microbacterium phage StrawberryJamm]],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.963, -2.707694805492614, no F: hypothetical protein SIF-BLAST: ,,[DNA recombinase [Microbacterium phage StrawberryJamm]],,QWY80134,98.2456,0.0 SIF-HHPRED: d.50.1.3 (A:) The homologous-pairing domain of Rad52 recombinase {Human (Homo sapiens) [TaxId: 9606]},,,d1kn0a_,43.6202,99.9 SIF-Syn: Same pham and position as Lonelysoil_50 and StrawberryJamm_54 and Zagie_51; are immediately downstream of exonuclease and upstream of HNH endonuclease. /note=Both glimmer and genemark called it as a gene, and agreed on the start. There is coding potential. There is an overlap of 4 bps, but it does not have the logest open reading frame. It does have the best vlues for the ribosomal binding site. All of the top BLAST hits are DNA recombinases. HHPred shows similar results.The homologs with similarity encode for DNA recombinases like Lonelysoil_50, StrawberryJamm_54, and Zagie_51. There is not enough evidence to assign it the function of RecA-like DNA recombinase or ASCE ATPase, so we have left it as NKF, since DNA recombinase is no longer an approved function. CDS complement (40172 - 40705) /gene="49" /product="gp49" /function="HNH endonuclease" /locus tag="FrankDeliGuy_49" /note=Original Glimmer call @bp 40705 has strength 10.62; Genemark calls start at 40705 /note=SSC: 40705-40172 CP: yes SCS: both ST: SS BLAST-Start: [HNH endonuclease [Microbacterium phage SallyK]],,NCBI, q1:s1 100.0% 1.44701E-126 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.794, -3.077027835973012, yes F: HNH endonuclease SIF-BLAST: ,,[HNH endonuclease [Microbacterium phage SallyK]],,WKW84919,100.0,1.44701E-126 SIF-HHPRED: zf-His_Me_endon ; Zinc-binding loop region of homing endonuclease,,,PF05551.14,38.9831,99.5 SIF-Syn: HNH endonuclease , same pham, and positional similarity as SallyK_50, Gazebo_50 and Squash_53, all downstream of a pham 41 gene and upstream of a pham 635 gene, except SallyK which has a pham 2 gene inbetween SallyK_50 and the downstream [jham 635 gene. /note=Both glimmer and genemark called it as a gene and agreed on the start as well. There is coding potential. There is a 4bp overlap, and it has the longest open frame, along with the best RBS values. The start is present in 38 MA`s and was found in 98.1% of the genes within the pham of this cluster. The gene results in a HNH endonuclease. SIF-BLAST showed top hits that encode HNH endonucleases. CDS complement (40702 - 41277) /gene="50" /product="gp50" /function="RuvC-like resolvase" /locus tag="FrankDeliGuy_50" /note=Original Glimmer call @bp 41277 has strength 13.39; Genemark calls start at 41277 /note=SSC: 41277-40702 CP: yes SCS: both ST: SS BLAST-Start: [RuvC-like resolvase [Microbacterium phage Zagie]],,NCBI, q1:s1 100.0% 4.4376E-134 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.658, -5.3604874223912145, no F: RuvC-like resolvase SIF-BLAST: ,,[RuvC-like resolvase [Microbacterium phage Zagie]],,UVG35407,98.4293,4.4376E-134 SIF-HHPRED: c.55.3.6 (A:) RuvC resolvase {Escherichia coli [TaxId: 562]},,,d1hjra_,86.911,99.9 SIF-Syn: RuvC-like resolvase, same pham and position as BabyDotz_53 & Zagie_54; BabyDotz_53 is 1 downstream and Zagie_54 is 2 downstream from HNH endonuclease /note=Start 41277 is supported by CP, SCS, multiple Blast 1:1 alignments with high coverage and low e-values, is SS, has the best RBS, is LO, and has a 4bp overlap. Its function is RuvC-like resolvase, supported by BLAST hits with the same function and high probabilities/low e-values, HHPred results with the same function and high probs/low e`s, and syteny with genes of the same function. There is no evidence supporting this as a membrane protein. CDS complement (41274 - 41504) /gene="51" /product="gp51" /function="hypothetical protein" /locus tag="FrankDeliGuy_51" /note=Original Glimmer call @bp 41504 has strength 7.66; Genemark calls start at 41504 /note=SSC: 41504-41274 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_STRAWBERRYJAMM_58 [Microbacterium phage StrawberryJamm] ],,NCBI, q1:s1 100.0% 2.80074E-47 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.47, -3.75071250087134, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_STRAWBERRYJAMM_58 [Microbacterium phage StrawberryJamm] ],,QWY80138,100.0,2.80074E-47 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function /note=Start 41504 is supported by GeneMark, Glimmer, Starterator, and multiple Blast 1:1 alignments with high coverage and low e-values. It also has CP, is the LO, has a 4bp overlap, and is the best RBS. This gene has NKF, as the BLAST results have NKF, there are no significant HHPred results, and syteny results had the same pham/location and also NKF. CDS complement (41501 - 41773) /gene="52" /product="gp52" /function="hypothetical protein" /locus tag="FrankDeliGuy_52" /note=Original Glimmer call @bp 41773 has strength 15.08; Genemark calls start at 41773 /note=SSC: 41773-41501 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_BABYDOTZ_55 [Microbacterium phage BabyDotz]],,NCBI, q1:s1 100.0% 5.96348E-55 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.625, -3.4283035164720754, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_BABYDOTZ_55 [Microbacterium phage BabyDotz]],,WNT45308,96.6667,5.96348E-55 SIF-HHPRED: Vs.4; Binds 3`, 3`-cGAMP, VIRAL PROTEIN; HET: 4BW; 2.0A {Tequatrovirus},,,7UQ2_A,75.5556,99.4 SIF-Syn: NKF, homologs have no known function /note=Start 41773 is supported by GeneMark, Glimmer, Starterator, and Blast, with multiple 1:1 alignments and high coverage/low e-values. It is also the LO, best RBS, and has CP. It has NKF, supported by BLAST alignments with NKF, no significant HHPred results with approved functions, and synteny with genes that also have NKF. CDS complement (41773 - 42165) /gene="53" /product="gp53" /function="MazG-like nucleotide pyrophosphohydrolase" /locus tag="FrankDeliGuy_53" /note=Original Glimmer call @bp 42165 has strength 13.14; Genemark calls start at 42165 /note=SSC: 42165-41773 CP: yes SCS: both ST: SS BLAST-Start: [MazG-like nucleotide pyrophosphohydrolase [Microbacterium phage Namago]],,NCBI, q1:s1 100.0% 8.99917E-80 GAP: 131 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.963, -3.1725816037952645, yes F: MazG-like nucleotide pyrophosphohydrolase SIF-BLAST: ,,[MazG-like nucleotide pyrophosphohydrolase [Microbacterium phage Namago]],,QQO39458,95.3488,8.99917E-80 SIF-HHPRED: a.204.1.2 (A:1-98) Hypothetical protein YpjD {Bacillus subtilis [TaxId: 1423]},,,d2gtaa1,81.5385,99.4 SIF-Syn: MazG-like nucleotide pyrophosphohydrolase, same pham and similar position as Judebell_58 and Namago_57, 5 genes upstream of RecA-like DNA recombinase and immediately downstream of SSB protein /note=Start 42165 is supported by GeneMark, Glimmer, Starterator, and multiple Blast 1:1 alignments with high coverages and low e-values. It also has CP and is the best RBS. Its function is MazG-like nucleotide pyrophosphohydrolase, supported by BLAST hits with the same function and high probabilities/low e-values, and synteny results with the same function and same surrounding genes. CDS complement (42297 - 43007) /gene="54" /product="gp54" /function="SSB protein" /locus tag="FrankDeliGuy_54" /note=Original Glimmer call @bp 43007 has strength 12.84; Genemark calls start at 43007 /note=SSC: 43007-42297 CP: yes SCS: both ST: SS BLAST-Start: [ssDNA binding protein [Microbacterium phage Grassboy]],,NCBI, q1:s1 100.0% 1.1711E-156 GAP: 1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.905, -2.827683592113848, yes F: SSB protein SIF-BLAST: ,,[ssDNA binding protein [Microbacterium phage Grassboy]],,UVG34317,98.3051,1.1711E-156 SIF-HHPRED: b.40.4.3 (A:) ssDNA-binding protein {Mycobacterium tuberculosis [TaxId: 1773]},,,d1ue1a_,48.3051,99.9 SIF-Syn: SSB protein, same pham and similar position as Grassboy_60 and Squash_62, immediately upstream of MazG-like nucleotide pyrophosphohydrolase and 4 genes upstream of RuvC-like resolvase. /note=Start 43007 is supported by GeneMark, Starterator, Glimmer, and multiple Blast 1:1 alignments with high coverage and low e-values. It also is the best RBS, LO, has CP, and has a small gap (1bp). As for its function, this gene is an SSB protein, supported by BLAST hits and HHPred results with the same function and high probabilities/low e-values, as well as synteny with genes with the same function and similar surrounding genes. CDS complement (43009 - 43188) /gene="55" /product="gp55" /function="hypothetical protein" /locus tag="FrankDeliGuy_55" /note=Original Glimmer call @bp 43188 has strength 19.26; Genemark calls start at 43188 /note=SSC: 43188-43009 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_GRASSBOY_61 [Microbacterium phage Grassboy]],,NCBI, q1:s1 100.0% 1.38384E-34 GAP: 11 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.06, -2.442961286954254, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_GRASSBOY_61 [Microbacterium phage Grassboy]],,UVG34318,100.0,1.38384E-34 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function. same pham and gene neighborhood as Fizzles_59, Grassboy_61, and Nike_61 /note=This is the most annotated start candidate, it is called by both Glimmer, Genemark, and Starterator. It has an 11 bp gap, and the best z-score and final score. The function appears to be a hypothetical protein as suggested by BLAST, HHPred and SIF-SYN, where there is no homologs with an associated function. This is not a membrane protein CDS complement (43200 - 43388) /gene="56" /product="gp56" /function="hypothetical protein" /locus tag="FrankDeliGuy_56" /note=Original Glimmer call @bp 43388 has strength 8.47; Genemark calls start at 43388 /note=SSC: 43388-43200 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CASEND_60 [Microbacterium phage Casend] ],,NCBI, q1:s1 100.0% 4.67503E-13 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.765, -5.200997464701116, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_60 [Microbacterium phage Casend] ],,QNL30027,70.7692,4.67503E-13 SIF-HHPRED: SIF-Syn: NKF, same pham and positional similarity as Casend_60 and Rudy_57, 2 genes upstream of ssDNA-binding protein /note=Start 43388 is called by Glimmer, Genemark and Starterator. It has an idela gap of -4 and the longest open reading frame. It has the 2nd best RBS scores, and has no known function as determined by BLAST, HH-Pred and SIF-SYN. It is not a membrane protein CDS complement (43385 - 43621) /gene="57" /product="gp57" /function="hypothetical protein" /locus tag="FrankDeliGuy_57" /note=Original Glimmer call @bp 43621 has strength 13.58; Genemark calls start at 43621 /note=SSC: 43621-43385 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CASEND_61 [Microbacterium phage Casend] ],,NCBI, q1:s1 100.0% 3.18285E-45 GAP: 71 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.295, -1.953940808934884, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_61 [Microbacterium phage Casend] ],,QNL30028,94.8718,3.18285E-45 SIF-HHPRED: SIF-Syn: NKF, same pham and positional similarity compared to Casend_61 and Rudy_58, 3 genes upstream of ssDNA-binding protein /note=Start 43621 was called by Glimmer, Genemark and Starterator. It has a large gap of 71 and is not the longest open reading frame. It has favorable RBS scores. It has no know function as indicated by BLAST, HH-Pred and SIF-SYN. It is not a membrane protein. CDS complement (43693 - 43803) /gene="58" /product="gp58" /function="hypothetical protein" /locus tag="FrankDeliGuy_58" /note=Original Glimmer call @bp 43845 has strength 1.25; Genemark calls start at 43803 /note=SSC: 43803-43693 CP: yes SCS: both-gm ST: SS BLAST-Start: [hypothetical protein [Microbacterium phage Judebell]],,NCBI, q2:s4 97.2222% 7.52179E-14 GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.538, -4.057188185093195, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein [Microbacterium phage Judebell]],,XCG97275,89.4737,7.52179E-14 SIF-HHPRED: SIF-Syn: NKF, same pham and positional similarity compared to Hyperion_61 and Judebell_62, 4 genes upstream of ssDNA binding protein /note=Start 43803 was selected by Genemark and Starterator, but not genemark, which called it as a gene with a different start. It has a small overlap of -8, and does not have the longest open reading frame. It has no known fucntion as indicated by BLAST, HH-Pred, and SIF-SYN. It is not a membrane protein CDS complement (43796 - 44122) /gene="59" /product="gp59" /function="hypothetical protein" /locus tag="FrankDeliGuy_59" /note=Original Glimmer call @bp 44122 has strength 5.37; Genemark calls start at 44122 /note=SSC: 44122-43796 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein SEA_ALUMINUMJESUS_59 [Microbacterium phage AluminumJesus]],,NCBI, q1:s1 51.8519% 1.00781E-9 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.549, -4.0338999058337315, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ALUMINUMJESUS_59 [Microbacterium phage AluminumJesus]],,UJD20794,53.4091,1.00781E-9 SIF-HHPRED: SIF-Syn: NKF - no positional similarity with closely-related phages /note=Start 44122 was selected by Glimmer, Genemark and Starterator. It has an overlap of -8 and the longest open reading frame. It has the best RBS scores. It has no known function as indicated by BLAST, HHpred and SIF-SYN, where it shared no positional similarity with similar genes. It is not a membrane protein CDS complement (44119 - 44370) /gene="60" /product="gp60" /function="hypothetical protein" /locus tag="FrankDeliGuy_60" /note=Original Glimmer call @bp 44370 has strength 6.79; Genemark calls start at 44370 /note=SSC: 44370-44119 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein SEA_ZAGIE_64 [Microbacterium phage Zagie]],,NCBI, q1:s1 100.0% 4.10513E-49 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.551, -5.646677400615975, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ZAGIE_64 [Microbacterium phage Zagie]],,UVG35417,98.7952,4.10513E-49 SIF-HHPRED: SIF-Syn: No significant results, no homologs had known functions /note=Start called by Glimmer, Genemark, and supported with MA`s in Starterator. Has the ideal overlap of -4 but has 4th best RBS. Has no known functions and no Transmembrane prediction. No significant results for CDS complement (44367 - 44540) /gene="61" /product="gp61" /function="hypothetical protein" /locus tag="FrankDeliGuy_61" /note=Original Glimmer call @bp 44510 has strength 5.89; Genemark calls start at 44540 /note=SSC: 44540-44367 CP: yes SCS: both-gm ST: NI BLAST-Start: [hypothetical protein SEA_CASEND_65 [Microbacterium phage Casend] ],,NCBI, q2:s3 98.2456% 1.65694E-23 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.424, -4.867713808513585, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_65 [Microbacterium phage Casend] ],,QNL30032,86.2069,1.65694E-23 SIF-HHPRED: SIF-Syn: NKF, no homologs have known functions but are from same pham (15) in Rudy_62 and Casend_65 /note=Start 44540 called by Glimmer, but genemark called a different start. It is not supported by Starterator, but hast the best gap/RBS. NKF according to BLAST, HHPred, and SYN. CDS complement (44537 - 44632) /gene="62" /product="gp62" /function="membrane protein" /locus tag="FrankDeliGuy_62" /note=Genemark calls start at 44629 /note=SSC: 44632-44537 CP: yes SCS: genemark-cs ST: NI BLAST-Start: [hypothetical protein SEA_CASEND_66 [Microbacterium phage Casend] ],,NCBI, q1:s1 100.0% 6.24124E-10 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.393, -3.8308929311341546, no F: membrane protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_66 [Microbacterium phage Casend] ],,QNL30033,96.875,6.24124E-10 SIF-HHPRED: SIF-Syn: NKF, compared Casend and Jehoshaphat all in same pham but homologs have no known function. /note=Deep TMHH provides Transmembrane prediction. /note=Most supported start called by Starterator (14MA`s) a different start was called by GeneMark but not Glimmer. Chosen start has best "gap" best RBS. TMHH indicates a membrane protein so chose that for SIF-BLAST. HHPred has one hit to "COX2-transmemb" with 90.3% coverage and a 0.35 e-value CDS complement (44629 - 44913) /gene="63" /product="gp63" /function="hypothetical protein" /locus tag="FrankDeliGuy_63" /note=Original Glimmer call @bp 44913 has strength 7.87; Genemark calls start at 44913 /note=SSC: 44913-44629 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein SEA_CASEND_67 [Microbacterium phage Casend] ],,NCBI, q1:s1 100.0% 2.00895E-60 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.776, -5.466930120988581, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_67 [Microbacterium phage Casend] ],,QNL30034,100.0,2.00895E-60 SIF-HHPRED: SIF-Syn: No homologs had known functions /note=Start 44913 called by Genemark, Glimmer, and has 25 MA`s in starterator. Doesn`t have the LORF or the best Z-score and Final score but has the ideal "gap" of -4 overlap and is a supported start. Compared Rudy_64 and Casend _67 which are from the same pham and neighborhood but homologs had no known functions. Deep TMHMM had no predictions. HHPred and SIF-Blast had no significant results/ no alignments. CDS complement (44910 - 45251) /gene="64" /product="gp64" /function="hypothetical protein" /locus tag="FrankDeliGuy_64" /note=Original Glimmer call @bp 45251 has strength 10.35; Genemark calls start at 45251 /note=SSC: 45251-44910 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 2.52656E-75 GAP: -19 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.48, -4.302115708322729, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein [Microbacterium phage Judebell]],,XCG97280,100.0,2.52656E-75 SIF-HHPRED: SIF-Syn: SIF-Blast, HHPred, and SYN had NKF no significant results and homologs had no known functions. Gene was in same pham as Casend_68 and Rudy_65, homologs have no known function /note=Start called by Glimmer and genemark at 45251 had only 1 MA in starterator whereas start at 45236 has 12 MA`s. If I were to use the start at 45236 it wouldn`t have the longest ORF or the best z and final score, but the start 45251, does have the LORF and the best Z and Final score despite it not being supported by starterator. Gap was also better for the start 45251. NKF for HHPred or Blast, no significant hits. TMHMM no predictions. CDS complement (45233 - 45379) /gene="65" /product="gp65" /function="hypothetical protein" /locus tag="FrankDeliGuy_65" /note=Original Glimmer call @bp 45379 has strength 5.97; Genemark calls start at 45379 /note=SSC: 45379-45233 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CASEND_69 [Microbacterium phage Casend] ],,NCBI, q1:s1 100.0% 4.92954E-25 GAP: 522 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.06, -2.442961286954254, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_69 [Microbacterium phage Casend] ],,QNL30036,100.0,4.92954E-25 SIF-HHPRED: SIF-Syn: Homologs have NKF. /note=Coding potential, Glimmer, Genemark, BLAST and starterator support this start. It is not the longest ORF, but it has the best RBS scores. BLAST, HHPred, and SIF-SYN support NKF. CDS complement (45902 - 47770) /gene="66" /product="gp66" /function="DNA primase/helicase" /locus tag="FrankDeliGuy_66" /note=Original Glimmer call @bp 47770 has strength 10.33; Genemark calls start at 47770 /note=SSC: 47770-45902 CP: yes SCS: both ST: SS BLAST-Start: [DNA primase/helicase [Microbacterium phage Casend] ],,NCBI, q1:s1 100.0% 0.0 GAP: 178 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.299, -6.188708875567495, no F: DNA primase/helicase SIF-BLAST: ,,[DNA primase/helicase [Microbacterium phage Casend] ],,QNL30037,99.8392,0.0 SIF-HHPRED: DNA primase/helicase; helicase, ATPase, hexamer, DNA replication, HYDROLASE, TRANSFERASE-DNA complex; HET: TTP; 3.2A {Enterobacteria phage T7},,,6N7I_C,57.8778,99.9 SIF-Syn: Same pham and position as Rudy_67 and Casend_70 /note=Coding potential, Glimmer, Genemark, BLAST and starterator support this start. It does not have the best RBS scores, but it is the longest ORF. BLAST, HHPred, and SIF-SYN support the function DNA primase/helicase. CDS complement (47949 - 49442) /gene="67" /product="gp67" /function="membrane protein" /locus tag="FrankDeliGuy_67" /note=Original Glimmer call @bp 49442 has strength 11.17; Genemark calls start at 49442 /note=SSC: 49442-47949 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_WHEELIE_70 [Microbacterium phage Wheelie]],,NCBI, q1:s1 100.0% 0.0 GAP: 133 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.963, -3.1725816037952645, yes F: membrane protein SIF-BLAST: ,,[hypothetical protein SEA_WHEELIE_70 [Microbacterium phage Wheelie]],,UVG34021,99.5976,0.0 SIF-HHPRED: SIF-Syn: Homologs have no known function, but are from same pham (52) and same neighborhood (Gazebo_71, Mashley _72, Wheelie_70) /note=Coding potential, Glimmer, Genemark, BLAST and starterator support this start. It is not the longest ORF, but it does have the best RBS scores. BLAST, HHPred, and SIF-SYN show NKF, but there was 1 domain found by both TMHMM and SOSUI. CDS complement (49576 - 49854) /gene="68" /product="gp68" /function="ribbon-helix-helix DNA binding domain" /locus tag="FrankDeliGuy_68" /note=Original Glimmer call @bp 49854 has strength 12.3; Genemark calls start at 49854 /note=SSC: 49854-49576 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CASEND_72 [Microbacterium phage Casend] ],,NCBI, q1:s1 100.0% 1.39918E-59 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.306, -2.0111200136961407, yes F: ribbon-helix-helix DNA binding domain SIF-BLAST: ,,[hypothetical protein SEA_CASEND_72 [Microbacterium phage Casend] ],,QNL30039,100.0,1.39918E-59 SIF-HHPRED: Putative; Helicobacter pylori, repressor, transcriptional regulator, DNA-binding, ribbon-helix-helix, HP0564, JHP0511, UNKNOWN FUNCTION, GENE REGULATION; NMR {Helicobacter pylori},,,2K1O_B,47.8261,97.9 SIF-Syn: Same pham and neighborhood as BabyDotz_69, Casend_72, and Jehoshaphat_73 /note=Coding potential, Glimmer, Genemark, Starterator, and BLAST support this start. It is also the longest ORF and has the best RBS scores. BLAST, HHPred, and SIF-SYN support the function as ribbon-helix-helix DNA binding domain protein. CDS complement (49851 - 50123) /gene="69" /product="gp69" /function="hypothetical protein" /locus tag="FrankDeliGuy_69" /note=Original Glimmer call @bp 50021 has strength 11.07; Genemark calls start at 50021 /note=SSC: 50123-49851 CP: yes SCS: both-cs ST: SS BLAST-Start: [hypothetical protein SEA_NAMAGO_73 [Microbacterium phage Namago] ],,NCBI, q1:s1 100.0% 1.48409E-55 GAP: -17 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.715, -6.546819551257786, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_NAMAGO_73 [Microbacterium phage Namago] ],,QQO39474,98.8889,1.48409E-55 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function; similar position and pham to BabyDotz_70 and Namago_73 /note=The start 50123 was not called by either Genemark or Glimmer, but it was supported by Starterator, BLAST with many more 1:1 alignments, and the ribosomal binding score. For the function, there was no evidence to strongly support a specific one, so it is NKF CDS complement (50107 - 50286) /gene="70" /product="gp70" /function="membrane protein" /locus tag="FrankDeliGuy_70" /note=Original Glimmer call @bp 50286 has strength 8.28; Genemark calls start at 50286 /note=SSC: 50286-50107 CP: yes SCS: both ST: SS BLAST-Start: [membrane protein [Microbacterium phage Namago]],,NCBI, q1:s1 100.0% 2.41476E-30 GAP: -17 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.74, -3.2495416658190135, yes F: membrane protein SIF-BLAST: ,,[membrane protein [Microbacterium phage Namago]],,QQO39475,96.6102,2.41476E-30 SIF-HHPRED: SIF-Syn: membrane protein, same pham and position as Namago_74 and SallyK_76; all are immediately downstream similar genes (share the same pham and position though they are all NKF) /note=The start 50286 was supported by both Glimmer and Genemark, as well as Starterator, BLAST, the open reading frame, and the ribosomal binding score. For the function, there was evidence of it being a membrane protein according to both TMHMM and SOSUI. /note=2 transmembrane region according to TMHMM and SOSUI CDS complement (50270 - 50611) /gene="71" /product="gp71" /function="hypothetical protein" /locus tag="FrankDeliGuy_71" /note=Original Glimmer call @bp 50611 has strength 14.36; Genemark calls start at 50611 /note=SSC: 50611-50270 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CASEND_75 [Microbacterium phage Casend] ],,NCBI, q1:s1 100.0% 9.96587E-75 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.48, -3.712290173411778, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_75 [Microbacterium phage Casend] ],,QNL30042,99.115,9.96587E-75 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function; similar pham and position to Casend_75 and Wheelie_74; downstream of a membrane protein /note=The start 50611 was supported by both Glimmer and Genemark, as well as Starterator, BLAST, the open reading frame, and the ribosomal binding score. As for the function, there is no strong evidence for a specific one, so it is NKF. CDS complement (50604 - 50918) /gene="72" /product="gp72" /function="hypothetical protein" /locus tag="FrankDeliGuy_72" /note=Original Glimmer call @bp 50918 has strength 14.34; Genemark calls start at 50918 /note=SSC: 50918-50604 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_GRASSBOY_79 [Microbacterium phage Grassboy]],,NCBI, q1:s1 100.0% 3.79153E-66 GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.915, -4.887586077132851, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_GRASSBOY_79 [Microbacterium phage Grassboy]],,UVG34336,97.1154,3.79153E-66 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function; similar position and pham to Grassboy_79 and Viceroy_74 /note=The start 50918 is supported by both Glimmer and Genemark, as well as Starterator, BLAST, and ribosomal binding score. There is no strong evidence for a specific function, so it is NKF. CDS complement (50911 - 51171) /gene="73" /product="gp73" /function="hypothetical protein" /locus tag="FrankDeliGuy_73" /note=Original Glimmer call @bp 51171 has strength 9.17; Genemark calls start at 51171 /note=SSC: 51171-50911 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_NAMAGO_78 [Microbacterium phage Namago]],,NCBI, q1:s1 100.0% 1.11091E-51 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.697, -3.1994059460302813, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_NAMAGO_78 [Microbacterium phage Namago]],,QQO39479,100.0,1.11091E-51 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function; similar position and pham to Namago_78 and Zagie_77 /note=The start 51171 was supported by both Glimmer and Genemark, as well as Starterator, BLAST, the gap length, and the ribosomal binding score. As for the function, there is not enough evidence to support a specifif one, so it is NKF. CDS complement (51168 - 51443) /gene="74" /product="gp74" /function="nucleotide pyrophosphohydrolase" /locus tag="FrankDeliGuy_74" /note=Original Glimmer call @bp 51443 has strength 14.11; Genemark calls start at 51443 /note=SSC: 51443-51168 CP: yes SCS: both ST: SS BLAST-Start: [nucleotide pyrophosphohydrolase [Microbacterium phage Hyperion] ],,NCBI, q1:s1 100.0% 8.30409E-53 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.14, -2.356391881054909, yes F: nucleotide pyrophosphohydrolase SIF-BLAST: ,,[nucleotide pyrophosphohydrolase [Microbacterium phage Hyperion] ],,YP_009801618,95.6044,8.30409E-53 SIF-HHPRED: SIF-Syn: Nucleotide Pyrophosphohydrolase, same pham and similar position as Hyperion_76 and Kelcole_84 /note=There is enough evidence to suggest that the function of FrankDeliGuy_76 is a nucleotide pyrophosphohydrolase.The start at position 51443 is supported by Starterator (highest number of manual annotations at 7, highly conserved in the EG cluster), was selected by both Glimmer and Genemark, has an ideal bp gap of -4, and has the best RBS score. This function is also supported by NCBI Blast where Microbacterium Phage Hyperion has a coverage of 100% and 1:1(Target:Query). CDS complement (51440 - 51694) /gene="75" /product="gp75" /function="hypothetical protein" /locus tag="FrankDeliGuy_75" /note=Original Glimmer call @bp 51694 has strength 15.81; Genemark calls start at 51694 /note=SSC: 51694-51440 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein HOT29_gp081 [Microbacterium phage Squash] ],,NCBI, q1:s1 100.0% 1.81913E-51 GAP: -14 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.393, -3.8932062518944837, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein HOT29_gp081 [Microbacterium phage Squash] ],,YP_009801820,96.4286,1.81913E-51 SIF-HHPRED: SIF-Syn: Squash_81, BabyDotz_76, Grassboy_82, same pham similar location, NKF /note=The start 51694 is supported by both Glimmer and Genemark, as well as Starterator, BLAST, and ribosomal binding score. Under NCBI Blast it has a 100% coverage and 1:1(Target:Query). As for the function, there is no strong evidence for a specific function, so it is NKF. CDS complement (51681 - 51968) /gene="76" /product="gp76" /function="membrane protein" /locus tag="FrankDeliGuy_76" /note=Original Glimmer call @bp 51962 has strength 15.05; Genemark calls start at 51959 /note=SSC: 51968-51681 CP: yes SCS: both-cs ST: SS BLAST-Start: [hypothetical protein SEA_NAMAGO_81 [Microbacterium phage Namago] ],,NCBI, q1:s1 100.0% 4.08155E-59 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 0.51, -7.891404326957996, no F: membrane protein SIF-BLAST: ,,[hypothetical protein SEA_NAMAGO_81 [Microbacterium phage Namago] ],,QQO39482,97.8947,4.08155E-59 SIF-HHPRED: SIF-Syn: membrane protein, same pham and position as Namago_81 and StrawberryJamm_84 /note=The start at position 51968 has coding potential and is supported by Starterator (highest number of manual annotations at 17), has an ideal bp gap of -4, has the LORF. Although, it has worst ribosomal binding score(RBS). This start was not called by both GeneMark and Glimmer. This start also supports the function being a membrane protein.There is some evidence to suggest that the function of FrankDeliGuy_78 is a membrane protein.1 membrane protein domain/regions found by TMHMM and this function is also supported by NCBI Blast (Microbacterium phage StrawberryJamm with a 100% coverage and 1:1(Target:Query), SIF-SYN. CDS complement (51965 - 52294) /gene="77" /product="gp77" /function="hypothetical protein" /locus tag="FrankDeliGuy_77" /note=Original Glimmer call @bp 52294 has strength 15.69; Genemark calls start at 52294 /note=SSC: 52294-51965 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CASEND_80 [Microbacterium phage Casend] ],,NCBI, q1:s1 100.0% 2.3566E-69 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.625, -3.4892599424135016, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_80 [Microbacterium phage Casend] ],,QNL30047,99.0826,2.3566E-69 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function; similar position and pham to Phabia_79 and Casend_80 /note=The start 52294 is supported by both Glimmer and Genemark, as well as Starterator, BLAST, and ribosomal binding score. Under NCBI Blast it has a 100% coverage and 1:1(Target:Query). As for the function, there is no strong evidence for a specific function, so it is NKF. CDS complement (52294 - 52623) /gene="78" /product="gp78" /function="hypothetical protein" /locus tag="FrankDeliGuy_78" /note=Original Glimmer call @bp 52623 has strength 10.78; Genemark calls start at 52623 /note=SSC: 52623-52294 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_ZAGIE_82 [Microbacterium phage Zagie]],,NCBI, q1:s1 100.0% 2.65706E-73 GAP: 24 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.06, -2.442961286954254, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ZAGIE_82 [Microbacterium phage Zagie]],,UVG35435,100.0,2.65706E-73 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function; similar position and pham to Zagie_82 and BabyDotz_79 /note=The start 52623 is supported by both Glimmer and Genemark, as well as Starterator, BLAST, and ribosomal binding score. Under NCBI Blast it has a 100% coverage with a 1:1(Target:Query). As for the function, there is no strong evidence for a specific function, so it is NKF. CDS complement (52648 - 52902) /gene="79" /product="gp79" /function="hypothetical protein" /locus tag="FrankDeliGuy_79" /note=Original Glimmer call @bp 52902 has strength 9.44; Genemark calls start at 52902 /note=SSC: 52902-52648 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_NIKE_81 [Microbacterium phage Nike]],,NCBI, q1:s1 100.0% 1.24049E-45 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.042, -4.702334767722353, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_NIKE_81 [Microbacterium phage Nike]],,QIQ63662,93.9759,1.24049E-45 SIF-HHPRED: SIF-Syn: NKF-same pham and location but have no known function /note=Start 52902 is SCS for both, has coding potential, is the supported start by Blast, has multiple 1:1 hits with 100% coverage and low e-values, has an ideal overlap of 4bp, LO, and best RBS. This gene has NKF, as supported by HHPred (all hits had low probibility and large e-values) and synteny (all genes in same pham and location NKF). CDS complement (52899 - 53405) /gene="80" /product="gp80" /function="hypothetical protein" /locus tag="FrankDeliGuy_80" /note=Original Glimmer call @bp 53405 has strength 12.06; Genemark calls start at 53405 /note=SSC: 53405-52899 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_SALLYK_85 [Microbacterium phage SallyK]],,NCBI, q1:s1 100.0% 8.16515E-113 GAP: 2 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.06, -3.3503726477288405, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_SALLYK_85 [Microbacterium phage SallyK]],,WKW84951,98.2143,8.16515E-113 SIF-HHPRED: SIF-Syn: NKF-same pham and location but have no known function /note=Although it is not the LO and has a gap of 2bp, start 53405 has coding potential, is the supported start, is SCS, has multiple 1:1 alignments with low e-values and high coverage, and is the best RBS, therefore supporting this start. This protein has NKF, as the BLAST alignments were hypothetical proteins, the Phamerator showed alignments also had NKF and were in same pham and location, and the HHPred results had low probabilities and high e-values. CDS complement (53408 - 53905) /gene="81" /product="gp81" /function="hypothetical protein" /locus tag="FrankDeliGuy_81" /note=Original Glimmer call @bp 53905 has strength 11.12; Genemark calls start at 53905 /note=SSC: 53905-53408 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_STRAWBERRYJAMM_90 [Microbacterium phage StrawberryJamm] ],,NCBI, q1:s1 100.0% 2.15434E-56 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.152, -4.332504169956899, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_STRAWBERRYJAMM_90 [Microbacterium phage StrawberryJamm] ],,QWY80168,80.5369,2.15434E-56 SIF-HHPRED: SIF-Syn: NKF-same pham and location but have no known function /note=Even though it`s not the LO, start 53095 has coding potential, supported by both SCS, is SS, has multiple 1:1 alignments with 100% coverage, has a 4bp overlap, and is the best RBS. It has NKF, which is supported by BLAST whose alignments also had NKF, HHPred with low probabilities and high e-values, and the synteny, which genes in same pham and location that also had NKF. CDS complement (53902 - 54090) /gene="82" /product="gp82" /function="hypothetical protein" /locus tag="FrankDeliGuy_82" /note=Original Glimmer call @bp 54090 has strength 7.25; Genemark calls start at 54090 /note=SSC: 54090-53902 CP: no SCS: both ST: SS BLAST-Start: GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.123, -4.744199388040119, no F: hypothetical protein SIF-BLAST: SIF-HHPRED: SIF-Syn: NKF-same pham and location but have no known function /note=Even though it`s not the LO, start 54090 has coding potential, supported by both SCS, is SS, has multiple 1:1 alignments with 100% coverage, has a 4bp overlap, and is the best RBS. It has NKF, which is supported by BLAST whose alignments also had NKF, HHPred with low probabilities and high e-values, and the synteny, which genes in same pham and location that also had NKF. CDS complement (54087 - 54410) /gene="83" /product="gp83" /function="hypothetical protein" /locus tag="FrankDeliGuy_83" /note=Original Glimmer call @bp 54419 has strength 12.52; Genemark calls start at 54419 /note=SSC: 54410-54087 CP: no SCS: both-cs ST: NI BLAST-Start: [hypothetical protein SEA_CASEND_86 [Microbacterium phage Casend] ],,NCBI, q1:s1 100.0% 5.59337E-73 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.502, -3.666183879767567, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_86 [Microbacterium phage Casend] ],,QNL30053,99.0654,5.59337E-73 SIF-HHPRED: SIF-Syn: NKF-same pham and location but have no known function /note=I changed the start site from the GeneMark and Glimmer supported start 54419 to 54410. I chose to change to this start because it has CP, is SS, has a 4 bp overlap, is the best RBS, and has multiple 1:1 alignments with high coverage and low e-values. Even though it is the LO, I did not choose start 54419 because it has a 13bp overlap, is not SS, and has no 1:1 Blast alignments. As for this genes function, it has NKF, which is supported by BLAST whose alignments had NKF, probabilities less than 90% and e-values greater than 0.1 in HHPred, and the syteny results showing genes in the same pham and location that also do not have a function. CDS complement (54407 - 54727) /gene="84" /product="gp84" /function="hypothetical protein" /locus tag="FrankDeliGuy_84" /note=Original Glimmer call @bp 54727 has strength 11.95; Genemark calls start at 54727 /note=SSC: 54727-54407 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHABIA_86 [Microbacterium phage Phabia] ],,NCBI, q1:s1 100.0% 5.49751E-68 GAP: 8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.151, -2.7806870294376242, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHABIA_86 [Microbacterium phage Phabia] ],,QQO39595,99.0566,5.49751E-68 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function /note=Start 54727 is supported by both Glimmer and GeneMark. There are multiple 1:1 alignments with high coverage and low e-values. It has the smallest gap and LORF. This gene has NKF. CDS complement (54736 - 55353) /gene="85" /product="gp85" /function="hypothetical protein" /locus tag="FrankDeliGuy_85" /note=Original Glimmer call @bp 55353 has strength 15.39; Genemark calls start at 55353 /note=SSC: 55353-54736 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CASEND_88 [Microbacterium phage Casend]],,NCBI, q1:s1 100.0% 1.85638E-133 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.47, -3.75071250087134, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_88 [Microbacterium phage Casend]],,QNL30055,93.7799,1.85638E-133 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function /note=Start 55353 is supported by both Glimmer and Genemark. It has multiple 1:1 alignments with high coverage and low e-values, best RBS, and having an ideal 4 bp overlap. From the results of BLAST, HHPRED, and Synteny there is no significant results. Leaving this gene to have no known function. CDS complement (55350 - 55967) /gene="86" /product="gp86" /function="hypothetical protein" /locus tag="FrankDeliGuy_86" /note=Original Glimmer call @bp 55967 has strength 14.87; Genemark calls start at 55928 /note=SSC: 55967-55350 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein SEA_CASEND_89 [Microbacterium phage Casend]],,NCBI, q1:s1 100.0% 3.72387E-151 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.182, -6.3511191392333295, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_89 [Microbacterium phage Casend]],,QNL30056,100.0,3.72387E-151 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function /note=Start 55967 is supported by Glimmer. It has multiple 1:1 alignments with high coverage and low e-values, best RBS, and having an ideal 4 bp overlap. From the results of BLAST, HHPRED, and Synteny there is no significant results. Leaving this gene to have no known function. CDS complement (55964 - 56212) /gene="87" /product="gp87" /function="hypothetical protein" /locus tag="FrankDeliGuy_87" /note=Original Glimmer call @bp 56212 has strength 12.74; Genemark calls start at 56212 /note=SSC: 56212-55964 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CASEND_90 [Microbacterium phage Casend] ],,NCBI, q1:s1 100.0% 8.78199E-52 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.639, -5.47994862755136, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_90 [Microbacterium phage Casend] ],,QNL30057,100.0,8.78199E-52 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function /note=Start 56212 is supported by both Glimmer and Genemark. It has multiple 1:1 alignments with high coverage and low e-values, best RBS, and having an ideal 1 bp overlap. The BLAST results have a top hit of Casend_90, NCBI, 1:1, 100%, 9e-52. From the results of BLAST, HHPRED, and Synteny there is no significant results. Leaving this gene to have no known function. CDS complement (56212 - 56640) /gene="88" /product="gp88" /function="hypothetical protein" /locus tag="FrankDeliGuy_88" /note=Original Glimmer call @bp 56640 has strength 9.08; Genemark calls start at 56640 /note=SSC: 56640-56212 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CASEND_91 [Microbacterium phage Casend]],,NCBI, q1:s1 100.0% 7.30154E-98 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.815, -3.0936172596596085, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_91 [Microbacterium phage Casend]],,QNL30058,100.0,7.30154E-98 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function /note=Start 56640 is supported by both Glimmer and Genemark. It has multiple 1:1 alignments with high coverage and low e-values, best RBS, and having an ideal 4 bp overlap. The BLAST results had a hit with Casend_91, NCBI, 1:1, 100%, 7e-98. From the results of BLAST, HHPRED, and Synteny there is no significant results. Leaving this gene to have no known function. CDS complement (56637 - 57206) /gene="89" /product="gp89" /function="hypothetical protein" /locus tag="FrankDeliGuy_89" /note=Original Glimmer call @bp 57206 has strength 9.92; Genemark calls start at 57206 /note=SSC: 57206-56637 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CASEND_92 [Microbacterium phage Casend]],,NCBI, q1:s1 100.0% 6.35326E-136 GAP: 112 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.043, -2.5410833118725082, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CASEND_92 [Microbacterium phage Casend]],,QNL30059,100.0,6.35326E-136 SIF-HHPRED: SIF-Syn: NKF- no positional similarity with closely-related phages /note=Start 57206 is supported by Glimmer, Genemark, Starterator, BLAST, has high coding potential, the LORF, the smallest gap and favorable RBS score. There is no known function for SIF-BLAST, where it has 1:1 alignments with other genes of no known function, SIF-HHPred, SIF-SYN,and it is not a membrane protein. CDS complement (57319 - 57492) /gene="90" /product="gp90" /function="hypothetical protein" /locus tag="FrankDeliGuy_90" /note=Original Glimmer call @bp 57492 has strength 5.41; Genemark calls start at 57492 /note=SSC: 57492-57319 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein HOT27_gp091 [Microbacterium phage Hyperion] ],,NCBI, q1:s1 100.0% 1.84096E-33 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.505, -3.738562143838384, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein HOT27_gp091 [Microbacterium phage Hyperion] ],,YP_009801633,100.0,1.84096E-33 SIF-HHPRED: SIF-Syn: NKF- no positional similarity with closely-related phages. /note=Start 57492 is supported by Glimmer, Genemark, Starterator, BLAST, has high coding potential, the LORF, an overlap of -4 and favorable RBS score. There is no known function for SIF-BLAST, where it has 1:1 alignments with other genes of no known function, SIF-HHPred, SIF-SYN,and it is not a membrane protein. CDS complement (57489 - 57716) /gene="91" /product="gp91" /function="hypothetical protein" /locus tag="FrankDeliGuy_91" /note=Original Glimmer call @bp 57716 has strength 9.84; Genemark calls start at 57716 /note=SSC: 57716-57489 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_NIKE_91 [Microbacterium phage Nike] ],,NCBI, q1:s1 100.0% 1.78312E-46 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.06, -2.5052746077145835, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_NIKE_91 [Microbacterium phage Nike] ],,QIQ63671,100.0,1.78312E-46 SIF-HHPRED: SIF-Syn: NKF- no positional similarity with closely-related phages /note=Start 57716 is supported by Glimmer, Genemark, Starterator, BLAST, has high coding potential, the LORF, an overlap of -8 and favorable RBS score. There is no known function for SIF-BLAST, where it has 1:1 alignments with other genes of no known function, SIF-HHPred, SIF-SYN,and it is not a membrane protein. CDS complement (57709 - 57873) /gene="92" /product="gp92" /function="hypothetical protein" /locus tag="FrankDeliGuy_92" /note=Original Glimmer call @bp 57873 has strength 13.92; Genemark calls start at 57873 /note=SSC: 57873-57709 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_ROWLF_91 [Microbacterium phage Rowlf]],,NCBI, q1:s1 100.0% 1.08782E-27 GAP: 51 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.06, -2.583959800616441, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ROWLF_91 [Microbacterium phage Rowlf]],,XEN17674,100.0,1.08782E-27 SIF-HHPRED: Tal_Pri; Tarsal-less (Tal), also known as polished rice (Pri), and related peptides. The tal/pri gene produces a single polycistronic transcript that encodes 4 related peptides: tal-1A, tal-2A, and tal-3A which are each 11 amino acids long, and tal-AA, which is 32 amino acids long, the shorter ones contain one conserved LDPTGXY motif, tal-AA contains two.,,,cd20258,35.1852,32.0 SIF-Syn: NKF- no positional similarity with closely-related phages /note=Start 57873 is supported by Glimmer, Genemark, Starterator, BLAST, has high coding potential, the LORF, the smallest gap of 51 and favorable RBS score. There is no known function for SIF-BLAST, where it has 1:1 alignments with other genes of no known fucntion, SIF-HHPred, SIF-SYN,and it is not a membrane protein. CDS complement (57925 - 58269) /gene="93" /product="gp93" /function="hypothetical protein" /locus tag="FrankDeliGuy_93" /note=Original Glimmer call @bp 58269 has strength 10.21; Genemark calls start at 58269 /note=SSC: 58269-57925 CP: yes SCS: both ST: NA BLAST-Start: GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.998, -5.003556626692295, no F: hypothetical protein SIF-BLAST: SIF-HHPRED: d.58.7.1 (A:177-284) Polypyrimidine tract-binding protein {Human (Homo sapiens) [TaxId: 9606]},,,d2adba1,40.3509,55.9 SIF-Syn: NKF- no positional similarity with closely-related phages /note=Start 58269 is supported by Glimmer, Genemark, Starterator, and has high coding potential. It has the smallest overlap of -4, but not the longest open reading frame or most favorable RBS scores. The function is undetermined, and there are no Blast results the gene is a singelton. CDS complement (58266 - 58523) /gene="94" /product="gp94" /function="hypothetical protein" /locus tag="FrankDeliGuy_94" /note=Original Glimmer call @bp 58523 has strength 11.72; Genemark calls start at 58523 /note=SSC: 58523-58266 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_ROWLF_93 [Microbacterium phage Rowlf]],,NCBI, q1:s1 100.0% 1.20588E-54 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.161, -4.455662434380964, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ROWLF_93 [Microbacterium phage Rowlf]],,XEN17676,100.0,1.20588E-54 SIF-HHPRED: LETM1_RBD ; LETM1-like, RBD,,,PF07766.16,37.6471,70.8 SIF-Syn: NKF- no positional similarity with closely-related phages /note=Start 58523 is supported by Glimmer, Genemark, Starterator, BLAST, has high coding potential, the LORF, the best overlap of -4 and favorable RBS score. There is no known function for SIF-BLAST, where it has 1:1 alignments with other genes of no known fucntion, SIF-HHPred, SIF-SYN,and it is not a membrane protein. CDS complement (58520 - 58657) /gene="95" /product="gp95" /function="membrane protein" /locus tag="FrankDeliGuy_95" /note=Original Glimmer call @bp 58657 has strength 14.0; Genemark calls start at 58657 /note=SSC: 58657-58520 CP: yes SCS: both ST: SS BLAST-Start: [membrane protein [Microbacterium phage StrawberryJamm] ],,NCBI, q1:s1 100.0% 9.89238E-16 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.124, -4.532304881730868, no F: membrane protein SIF-BLAST: ,,[membrane protein [Microbacterium phage StrawberryJamm] ],,QWY80180,95.5556,9.89238E-16 SIF-HHPRED: SIF-Syn: NKF- homologs have no known function /note=membrane protein, 2 domains found by both TMHMM and SOSUI /note= /note=Start 58657 is supported by Glimmer, Genemark, Starterator, BLAST, has high coding potential, has the best overlap of 4bp and a favorable RBS score. It does not have the largest open reading frame. SIF-Blast aligns with membrane proteins, which is supported by DeepTMHMM & SOSUI. HHPred and SIF-SYN have NKF. CDS complement (58654 - 59085) /gene="96" /product="gp96" /function="hypothetical protein" /locus tag="FrankDeliGuy_96" /note=Original Glimmer call @bp 59085 has strength 8.39; Genemark calls start at 59085 /note=SSC: 59085-58654 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 1.62779E-94 GAP: 33 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.499, -4.43822993146257, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein [Microbacterium phage Judebell]],,XCG97310,97.2028,1.62779E-94 SIF-HHPRED: SIF-Syn: NKF- homologs have no known function /note=Start 59085 is supported by Glimmer, Genemark, Starterator, Blast, has high coding potential, the LORF, the smallest gap of 33bp and the best RBS scores. There is no known function for 1:1 aligned Blast results, SIF-HHPred, SIF-SYN support NKF, and it is not a membrane protein. CDS complement (59119 - 59400) /gene="97" /product="gp97" /function="hypothetical protein" /locus tag="FrankDeliGuy_97" /note=Original Glimmer call @bp 59400 has strength 13.28; Genemark calls start at 59400 /note=SSC: 59400-59119 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 2.73188E-62 GAP: 37 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.74, -3.1085431521568267, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein [Microbacterium phage Judebell]],,XCG97311,100.0,2.73188E-62 SIF-HHPRED: SIF-Syn: NKF- homologs have no known function /note=Start 59400 is supported by Glimmer, Genemark, Starterator, BLAST, has high coding potential, the LORF, and favorable RBS scores. There is no known function for SIF-BLAST, where it has 1:1 alignments with other genes of no known function, SIF-HHPred, SIF-SYN support NKF, and it is not a membrane protein. CDS complement (59438 - 59788) /gene="98" /product="gp98" /function="hypothetical protein" /locus tag="FrankDeliGuy_98" /note=Original Glimmer call @bp 59788 has strength 13.84; Genemark calls start at 59788 /note=SSC: 59788-59438 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein HOT29_gp102 [Microbacterium phage Squash] ],,NCBI, q1:s1 100.0% 5.62962E-79 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.49, -3.6907860541164537, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein HOT29_gp102 [Microbacterium phage Squash] ],,YP_009801841,100.0,5.62962E-79 SIF-HHPRED: SIF-Syn: NKF- homologs have no known function /note=Start 59788 is supported by Glimmer, Genemark, Starterator, BLAST, has high coding potential, the LORF, the smallest gap of 426 and favorable RBS score. There is no known function for SIF-BLAST, where it has 1:1 alignments with other genes of no known function, SIF-HHPred, SIF-SYN,and it is not a membrane protein. CDS complement (59785 - 60216) /gene="99" /product="gp99" /function="hypothetical protein" /locus tag="FrankDeliGuy_99" /note=Original Glimmer call @bp 60216 has strength 10.52; Genemark calls start at 60216 /note=SSC: 60216-59785 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_ROWLF_99 [Microbacterium phage Rowlf]],,NCBI, q1:s1 100.0% 1.48231E-94 GAP: 45 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.161, -4.841864039781758, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ROWLF_99 [Microbacterium phage Rowlf]],,XEN17682,99.3007,1.48231E-94 SIF-HHPRED: SIF-Syn: NKF- homologs have no known function /note=Start at 6021 supported by Coding potential, GeneMark, Glimmer, Starterator, BLAST, RBS scores, and it is the longest ORF . It has a large gap but there is a lot of evidence for this start. BLAST, HHPred, and SYN support NKF. CDS complement (60262 - 60351) /gene="100" /product="gp100" /function="hypothetical protein" /locus tag="FrankDeliGuy_100" /note=Genemark calls start at 60351 /note=SSC: 60351-60262 CP: yes SCS: genemark ST: SS BLAST-Start: [hypothetical protein [Microbacterium phage Judebell]],,NCBI, q1:s1 100.0% 6.29273E-11 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.362, -4.996601827017273, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein [Microbacterium phage Judebell]],,XCG97314,100.0,6.29273E-11 SIF-HHPRED: SIF-Syn: NKF - homologs have no known function /note=This start has coding potential and is supported by genemark and starterator with 25 manual annotations. It also has a great gap score of -1, is the longest ORF, and has the best RBS scores, which support this start. BLAST, HHPred (insufficient percent coverage, probability, and e value), and SYN support NKF. CDS complement (60351 - 60497) /gene="101" /product="gp101" /function="hypothetical protein" /locus tag="FrankDeliGuy_101" /note=Original Glimmer call @bp 60497 has strength 5.94 /note=SSC: 60497-60351 CP: no SCS: glimmer ST: SS BLAST-Start: [hypothetical protein SEA_RUDY_102 [Microbacterium phage Rudy] ],,NCBI, q1:s1 100.0% 9.07448E-26 GAP: 23 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.74, -3.1085431521568267, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_RUDY_102 [Microbacterium phage Rudy] ],,QQO39284,100.0,9.07448E-26 SIF-HHPRED: SIF-Syn: NKF- homologs have no known function /note=Only Glimmer called this start, but this start had the best RBS scores, and starterator showed the highest manual annotations (17). HHPred revealed no known function as % coverage and probability were insufficient along with a high e-value. NCBI blast had high percent coverage and 1:1 alignments, however, there are no known functions to justify. CDS complement (60521 - 60634) /gene="102" /product="gp102" /function="hypothetical protein" /locus tag="FrankDeliGuy_102" /note=Original Glimmer call @bp 60634 has strength 4.02 /note=SSC: 60634-60521 CP: no SCS: glimmer ST: SS BLAST-Start: [hypothetical protein SEA_NAMAGO_105 [Microbacterium phage Namago]],,NCBI, q1:s1 100.0% 6.65187E-18 GAP: 36 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.643, -3.3910054376556182, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_NAMAGO_105 [Microbacterium phage Namago]],,QQO39506,100.0,6.65187E-18 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function /note=This start is supported by glimmer and starterator and also has the longest open reading frame with good RBS scores. There is no coding potential, and BLAST, HHPred (insufficient percent coverage and e value), and SYN support NKF. No evidence for a membrane protein by DeepTMHMM CDS complement (60671 - 60988) /gene="103" /product="gp103" /function="hypothetical protein" /locus tag="FrankDeliGuy_103" /note=Original Glimmer call @bp 60988 has strength 4.16; Genemark calls start at 60988 /note=SSC: 60988-60671 CP: yes SCS: both ST: SS BLAST-Start: [membrane protein [Microbacterium phage StrawberryJamm] ],,NCBI, q1:s1 100.0% 4.43152E-64 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.91, -4.897971560974249, no F: hypothetical protein SIF-BLAST: ,,[membrane protein [Microbacterium phage StrawberryJamm] ],,QWY80188,99.0291,4.43152E-64 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function /note=This start has coding potential and gene mark, glimmer, starterator, BLAST, and the 4 bp overlap support this start. It is not the longest ORF and has the second-best RBS scores but all other evidence favors this start. BLAST and TmHMM show membrane protein. CDS complement (60985 - 61200) /gene="104" /product="gp104" /function="hypothetical protein" /locus tag="FrankDeliGuy_104" /note=Original Glimmer call @bp 61200 has strength 6.69; Genemark calls start at 61200 /note=SSC: 61200-60985 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_STRAWBERRYJAMM_112 [Microbacterium phage StrawberryJamm] ],,NCBI, q1:s1 100.0% 4.39675E-38 GAP: 185 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.755, -5.238109312333945, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_STRAWBERRYJAMM_112 [Microbacterium phage StrawberryJamm] ],,QWY80189,98.5916,4.39675E-38 SIF-HHPRED: SIF-Syn: NKF- homologs have no known function /note=This start has coding potential and is supported by gene mark, glimmer, and starterator with 27 manual annotations. It is the longest ORF, and RBS scores (2nd best) support this start. There is a large gap but evidence favors this start. BLAST, HHPred, and SYN support NKF. No evidence for a membrane protein by DeepTMHMM CDS complement (61386 - 62081) /gene="105" /product="gp105" /function="hypothetical protein" /locus tag="FrankDeliGuy_105" /note=Original Glimmer call @bp 62081 has strength 10.52; Genemark calls start at 62081 /note=SSC: 62081-61386 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_ROWLF_104 [Microbacterium phage Rowlf]],,NCBI, q1:s1 100.0% 5.81735E-155 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.91, -5.4877970958852, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ROWLF_104 [Microbacterium phage Rowlf]],,XEN17687,98.7013,5.81735E-155 SIF-HHPRED: SIF-Syn: NKF, homologs have no known function /note=This start has coding potential and is supported by genemark, glimmer, and starterator with 32 manual annotations. It is the longest ORF and has a large gap with good RBS scores (second best) but all other evidence favors this start. BLAST, HHPred (insufficient percent coverage, probability, and e value) and SYN support NKF.