CDS 41 - 379 /gene="1" /product="gp1" /function="Hypothetical Protein" /locus tag="GoldenBlanche_1" /note=Original Glimmer call @bp 41 has strength 17.44; Genemark calls start at 41 /note=SSC: 41-379 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein JTF51_gp01 [Microbacterium phage PaoPu] ],,NCBI, q1:s1 100.0% 5.84666E-73 GAP: 0 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.952, -5.035742295039811, yes F: Hypothetical Protein SIF-BLAST: ,,[hypothetical protein JTF51_gp01 [Microbacterium phage PaoPu] ],,YP_009996586,100.0,5.84666E-73 SIF-HHPRED: SIF-Syn: First gene, NKF, upstream NKF /note=Most called start - good z score - aligns with genemark CP CDS 376 - 1071 /gene="2" /product="gp2" /function="Hypothetical Protein" /locus tag="GoldenBlanche_2" /note=Original Glimmer call @bp 376 has strength 9.34; Genemark calls start at 376 /note=SSC: 376-1071 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_MINIMA_2 [Microbacterium phage Minima] ],,NCBI, q1:s1 100.0% 5.94408E-161 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.213, -4.497802523441791, no F: Hypothetical Protein SIF-BLAST: ,,[hypothetical protein SEA_MINIMA_2 [Microbacterium phage Minima] ],,AXQ64351,100.0,5.94408E-161 SIF-HHPRED: SIF-Syn: Downstream NKF, upstream terminase /note=Most annotated start, aligns with GM CP CDS 1074 - 2534 /gene="3" /product="gp3" /function="terminase" /locus tag="GoldenBlanche_3" /note=Original Glimmer call @bp 1074 has strength 12.75; Genemark calls start at 1074 /note=SSC: 1074-2534 CP: yes SCS: both ST: NA BLAST-Start: [terminase [Microbacterium phage PaoPu] ],,NCBI, q1:s1 100.0% 0.0 GAP: 2 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.76, -5.98696854491312, no F: terminase SIF-BLAST: ,,[terminase [Microbacterium phage PaoPu] ],,YP_009996588,100.0,0.0 SIF-HHPRED: Terminase large subunit; genome packaging, bacteriophage, ATPase, nuclease, VIRAL PROTEIN; HET: BR; 2.2A {Enterobacteria phage HK97},,,6Z6D_A,93.4156,100.0 SIF-Syn: Downstream NKF, upstream portal protein /note=Does not have most annotated start. Predicted start is earliest possible and includes CP CDS 2659 - 3786 /gene="4" /product="gp4" /function="portal protein" /locus tag="GoldenBlanche_4" /note=Original Glimmer call @bp 2659 has strength 12.98; Genemark calls start at 2728 /note=SSC: 2659-3786 CP: yes SCS: both-gl ST: SS BLAST-Start: [portal protein [Microbacterium phage PaoPu] ],,NCBI, q1:s1 100.0% 0.0 GAP: 124 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.872, -5.756015422186926, no F: portal protein SIF-BLAST: ,,[portal protein [Microbacterium phage PaoPu] ],,YP_009996589,100.0,0.0 SIF-HHPRED: Portal protein; Prohead I, icosahedral symmetry, HK97, phage, capsid, VIRUS; 3.6A {Escherichia phage HK97},,,8FQL_L,89.0667,100.0 SIF-Syn: Downstream from terminase, upstream major capsid and protease fusion. /note=Suggested start, glimmer predicted. This start is earlier than GM coding potential (2728 start aligns best with CP - but z score is much worse). GM start does not include all CP. CDS 3783 - 5369 /gene="5" /product="gp5" /function="major capsid and protease fusion protein" /locus tag="GoldenBlanche_5" /note=Original Glimmer call @bp 3783 has strength 15.79; Genemark calls start at 3783 /note=SSC: 3783-5369 CP: yes SCS: both ST: SS BLAST-Start: [major capsid and protease fusion protein [Microbacterium phage Belthelas]],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.962, -3.5088110057482766, yes F: major capsid and protease fusion protein SIF-BLAST: ,,[major capsid and protease fusion protein [Microbacterium phage Belthelas]],,QDF18032,98.4848,0.0 SIF-HHPRED: Major capsid protein Rcc01687; "capsid", "jelly roll", "spike", "HK97", VIRUS; 3.42A {Rhodobacter capsulatus DE442},,,6TSU_Z4,54.7348,99.9 SIF-Syn: Downstream portal protein, upstream head-to-tail adapter /note=Suggested start CDS 5373 - 5726 /gene="6" /product="gp6" /function="head-to-tail adaptor" /locus tag="GoldenBlanche_6" /note=Original Glimmer call @bp 5373 has strength 19.65; Genemark calls start at 5373 /note=SSC: 5373-5726 CP: yes SCS: both ST: SS BLAST-Start: [head-to-tail adaptor [Microbacterium phage Jahseh]],,NCBI, q1:s1 100.0% 8.07842E-78 GAP: 3 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.55, -4.552792845506871, yes F: head-to-tail adaptor SIF-BLAST: ,,[head-to-tail adaptor [Microbacterium phage Jahseh]],,QKY79373,99.1453,8.07842E-78 SIF-HHPRED: Phage_connect_1 ; Phage gp6-like head-tail connector protein,,,PF05135.16,84.6154,99.5 SIF-Syn: downstream major capsid and protease fusion protein, upstream of tail terminator /note=Earliest possible and suggested start CDS 5723 - 6103 /gene="7" /product="gp7" /function="tail terminator" /locus tag="GoldenBlanche_7" /note=Original Glimmer call @bp 5723 has strength 23.03; Genemark calls start at 5723 /note=SSC: 5723-6103 CP: yes SCS: both ST: SS BLAST-Start: [tail terminator [Microbacterium phage Loca] ],,NCBI, q1:s1 100.0% 8.14735E-83 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.429, -6.591314556970386, no F: tail terminator SIF-BLAST: ,,[tail terminator [Microbacterium phage Loca] ],,URM86191,99.2064,8.14735E-83 SIF-HHPRED: Minor tail protein U; Mixed Alpha-Beta fold, VIRAL PROTEIN; HET: SO4, MSE; 2.7A {Enterobacteria phage lambda} SCOP: d.323.1.1,,,3FZ2_C,97.619,98.2 SIF-Syn: downstream head-to-tail adaptor, upstream major tail protein /note=most annotated and earliest possible start CDS 6142 - 6576 /gene="8" /product="gp8" /function="major tail protein" /locus tag="GoldenBlanche_8" /note=Original Glimmer call @bp 6142 has strength 16.64; Genemark calls start at 6142 /note=SSC: 6142-6576 CP: yes SCS: both ST: SS BLAST-Start: [major tail protein [Microbacterium phage PaoPu] ],,NCBI, q1:s1 100.0% 5.9374E-100 GAP: 38 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.443, -5.796882727134779, no F: major tail protein SIF-BLAST: ,,[major tail protein [Microbacterium phage PaoPu] ],,YP_009996593,100.0,5.9374E-100 SIF-HHPRED: Phage major tail protein, TP901-1 family; "neck", "portal", "capsid", "tail tube", VIRUS; 3.58A {Rhodobacter capsulatus},,,6TE9_G,96.5278,99.3 SIF-Syn: Downstream tail terminator, upstream NKF /note=earliest and SS CDS 6589 - 6972 /gene="9" /product="gp9" /function="Hypothetical Protein" /locus tag="GoldenBlanche_9" /note=Original Glimmer call @bp 6589 has strength 15.6; Genemark calls start at 6589 /note=SSC: 6589-6972 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein JTF51_gp09 [Microbacterium phage PaoPu] ],,NCBI, q1:s1 100.0% 3.51185E-85 GAP: 12 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.141, -4.294054598558697, yes F: Hypothetical Protein SIF-BLAST: ,,[hypothetical protein JTF51_gp09 [Microbacterium phage PaoPu] ],,YP_009996594,100.0,3.51185E-85 SIF-HHPRED: Minor_capsid_2 ; Minor capsid protein,,,PF11114.11,74.8031,99.4 SIF-Syn: Downstream major tail protein, upstream tail assembly chaperone /note=Earliest and most called start CDS 6986 - 7306 /gene="10" /product="gp10" /function="tail assembly chaperone" /locus tag="GoldenBlanche_10" /note=Original Glimmer call @bp 6986 has strength 16.1; Genemark calls start at 6986 /note=SSC: 6986-7306 CP: yes SCS: both ST: SS BLAST-Start: [tail assembly chaperone [Microbacterium phage PaoPu] ],,NCBI, q1:s1 100.0% 4.54361E-68 GAP: 13 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.368, -3.8256434426994073, yes F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Microbacterium phage PaoPu] ],,YP_009996596,100.0,4.54361E-68 SIF-HHPRED: SIF-Syn: Downstream HP, upstream tail assembly protein #2 (frameshift) /note=Earliest and most called start CDS join(6986..7270,7270..7422) /gene="11" /product="gp11" /function="tail assembly chaperone" /locus tag="GoldenBlanche_11" /note= /note=SSC: 6986-7422 CP: yes SCS: neither ST: NI BLAST-Start: GAP: -321 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.368, -3.8256434426994073, yes F: tail assembly chaperone SIF-BLAST: SIF-HHPRED: SIF-Syn: upstream tape measure protein /note=Part two of the PTFS /note=Slip at SSAEG - N or K with sequence GGGAAAC /note=Slip @ 7270 CDS 7540 - 9645 /gene="12" /product="gp12" /function="tape measure protein" /locus tag="GoldenBlanche_12" /note=Original Glimmer call @bp 7540 has strength 16.17; Genemark calls start at 7540 /note=SSC: 7540-9645 CP: yes SCS: both ST: SS BLAST-Start: [tape measure protein [Microbacterium phage Jannah]],,NCBI, q1:s1 100.0% 0.0 GAP: 116 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.631, -4.386181351886765, yes F: tape measure protein SIF-BLAST: ,,[tape measure protein [Microbacterium phage Jannah]],,QYW07171,99.572,0.0 SIF-HHPRED: Tape Measure Protein, gp57; phage tail, tail tip, tape measure protein, VIRAL PROTEIN; 3.7A {Staphylococcus virus 80alpha},,,6V8I_CF,19.4009,99.9 SIF-Syn: downstream tail assembly chaperones, upstream minor tail protein /note=Earliest possible and suggested start tRNA 9402 - 9476 /gene="13" /product="tRNA-Leu(gag)" /locus tag="GOLDENBLANCHE_13" /note=tRNA-Leu(gag) CDS 9642 - 10601 /gene="14" /product="gp14" /function="minor tail protein" /locus tag="GoldenBlanche_14" /note=Original Glimmer call @bp 9642 has strength 10.19; Genemark calls start at 9642 /note=SSC: 9642-10601 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Microbacterium phage PaoPu] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.904, -4.924073590240254, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage PaoPu] ],,YP_009996598,100.0,0.0 SIF-HHPRED: b.18.1.0 (A:) automated matches {Paenibacillus barcinonensis [TaxId: 198119]} | CLASS: All beta proteins, FOLD: Galactose-binding domain-like, SUPFAM: Galactose-binding domain-like, FAM: automated matches,,,SCOP_d4xuoa_,46.395,98.8 SIF-Syn: Downstream tape measure, upstream minor tail /note=Earliest possible and SS CDS 10601 - 12670 /gene="15" /product="gp15" /function="minor tail protein" /locus tag="GoldenBlanche_15" /note=Original Glimmer call @bp 10601 has strength 15.64; Genemark calls start at 10601 /note=SSC: 10601-12670 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Microbacterium phage PaoPu] ],,NCBI, q1:s1 100.0% 0.0 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.192, -5.097661467667712, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage PaoPu] ],,YP_009996599,100.0,0.0 SIF-HHPRED: Fibronectin; FN3 DOMAIN, FIBRONECTIN, CELL ADHESION; 1.754A {Homo sapiens},,,6MFA_A,52.2496,99.7 SIF-Syn: downstream minor tail protein, upstream minor tail protein /note=Earliest possible and suggested start CDS 12672 - 13220 /gene="16" /product="gp16" /function="minor tail protein" /locus tag="GoldenBlanche_16" /note=Original Glimmer call @bp 12672 has strength 19.06; Genemark calls start at 12672 /note=SSC: 12672-13220 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Microbacterium phage BurtonThePup] ],,NCBI, q1:s1 100.0% 1.01195E-126 GAP: 1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.131, -2.394485424036831, yes F: minor tail protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage BurtonThePup] ],,AVR56192,99.4505,1.01195E-126 SIF-HHPRED: Short tail fiber protein; STRUCTURAL PROTEIN; 12.0A {Enterobacteria phage T4} SCOP: i.19.1.1,,,1PDI_K,86.2637,97.8 SIF-Syn: downstream minor tail protein, upstream HP /note=earliest possible and suggested start CDS 13257 - 13544 /gene="17" /product="gp17" /function="Hypothetical Protein" /locus tag="GoldenBlanche_17" /note=Original Glimmer call @bp 13257 has strength 15.56; Genemark calls start at 13233 /note=SSC: 13257-13544 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein JTF51_gp16 [Microbacterium phage PaoPu]],,NCBI, q1:s9 100.0% 5.64607E-56 GAP: 36 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.522, -3.588526034455651, yes F: Hypothetical Protein SIF-BLAST: ,,[hypothetical protein JTF51_gp16 [Microbacterium phage PaoPu]],,YP_009996601,92.233,5.64607E-56 SIF-HHPRED: SIF-Syn: Downstream minor tail protein, upstream endolysin /note=Suggested start - aligns with GM coding potential CDS 13563 - 14249 /gene="18" /product="gp18" /function="endolysin" /locus tag="GoldenBlanche_18" /note=Original Glimmer call @bp 13563 has strength 14.51; Genemark calls start at 13563 /note=SSC: 13563-14249 CP: yes SCS: both ST: SS BLAST-Start: [endolysin [Microbacterium phage Dongwon] ],,NCBI, q1:s1 100.0% 3.56946E-164 GAP: 18 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.363, -3.9776535502172963, yes F: endolysin SIF-BLAST: ,,[endolysin [Microbacterium phage Dongwon] ],,AYJ73781,100.0,3.56946E-164 SIF-HHPRED: SIF-Syn: downstream HP and upstream HP /note=Earliest possible and suggested start CDS 14216 - 14479 /gene="19" /product="gp19" /function="membrane protein" /locus tag="GoldenBlanche_19" /note=Original Glimmer call @bp 14216 has strength 11.16; Genemark calls start at 14216 /note=SSC: 14216-14479 CP: yes SCS: both ST: SS BLAST-Start: [membrane protein [Microbacterium phage PaoPu] ],,NCBI, q1:s1 100.0% 4.99803E-58 GAP: -34 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.497, -6.723716915074367, no F: membrane protein SIF-BLAST: ,,[membrane protein [Microbacterium phage PaoPu] ],,AVR56410,100.0,4.99803E-58 SIF-HHPRED: SIF-Syn: Downstream endolysin, upstream membrane protein /note=Earliest possible and suggested start, later starts do not include all CP CDS 14476 - 14700 /gene="20" /product="gp20" /function="membrane protein" /locus tag="GoldenBlanche_20" /note=Original Glimmer call @bp 14476 has strength 13.43; Genemark calls start at 14476 /note=SSC: 14476-14700 CP: yes SCS: both ST: SS BLAST-Start: [holin [Microbacterium phage PaoPu] ],,NCBI, q1:s1 100.0% 1.4203E-43 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.716, -3.170856472917156, yes F: membrane protein SIF-BLAST: ,,[holin [Microbacterium phage PaoPu] ],,YP_009996604,100.0,1.4203E-43 SIF-HHPRED: Phage_holin_7_1 ; Mycobacterial 2 TMS Phage Holin (M2 Hol) Family,,,PF16081.8,62.1622,97.0 SIF-Syn: downstream membrane protein, upstream LSR2 like DNA bridging protein /note=earliest possible and SS /note= /note=Assigned membrane protein instead of holin because of adjacent membrane protein, lack of conserved domain evidence. There are two domains predicted and others have called holin. CDS complement (14769 - 14981) /gene="21" /product="gp21" /function="Lsr2-like DNA bridging protein" /locus tag="GoldenBlanche_21" /note=Original Glimmer call @bp 14981 has strength 11.07; Genemark calls start at 14981 /note=SSC: 14981-14769 CP: yes SCS: both ST: SS BLAST-Start: [Lsr2-like DNA bridging protein [Microbacterium phage PaoPu] ],,NCBI, q1:s1 100.0% 1.36018E-41 GAP: 2 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.533, -3.627004739933808, yes F: Lsr2-like DNA bridging protein SIF-BLAST: ,,[Lsr2-like DNA bridging protein [Microbacterium phage PaoPu] ],,YP_009996605,100.0,1.36018E-41 SIF-HHPRED: Protein lsr2; anti-parallel beta sheet, dimer, DNA BINDING PROTEIN; 1.728A {Mycobacterium tuberculosis},,,4E1P_B,84.2857,99.9 SIF-Syn: downstream membrane protein and upstream helix-turn-helix /note=suggested and earliest possible start CDS complement (14984 - 15484) /gene="22" /product="gp22" /function="helix-turn-helix DNA binding domain" /locus tag="GoldenBlanche_22" /note=Original Glimmer call @bp 15421 has strength 13.51; Genemark calls start at 15484 /note=SSC: 15484-14984 CP: yes SCS: both-gm ST: SS BLAST-Start: [DNA binding protein [Microbacterium phage PaoPu] ],,NCBI, q1:s1 100.0% 1.83669E-115 GAP: 79 bp gap LO: no RBS: Kibler 6, Karlin Medium, 0.44, -7.945159473550231, no F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[DNA binding protein [Microbacterium phage PaoPu] ],,YP_009996606,100.0,1.83669E-115 SIF-HHPRED: AM32; GRAM-POSITIVE, CONJUGATION, HELIX-TURN-HELIX, DSDNA BINDING, DNA BINDING PROTEIN; HET: MSE; 1.35A {Enterococcus faecalis},,,4P0Z_A,80.7229,99.0 SIF-Syn: downstream LSR2 like and upstream helix-turn-helix DNA binding protein /note=Chose genemark start which is SS. CDS complement (15564 - 15794) /gene="23" /product="gp23" /function="helix-turn-helix DNA binding domain" /locus tag="GoldenBlanche_23" /note=Original Glimmer call @bp 15794 has strength 4.84; Genemark calls start at 15794 /note=SSC: 15794-15564 CP: yes SCS: both ST: SS BLAST-Start: [helix-turn-helix DNA binding domain protein [Microbacterium phage Gardevoir] ],,NCBI, q1:s1 100.0% 7.80948E-45 GAP: 509 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 0.863, -7.838780790165433, no F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding domain protein [Microbacterium phage Gardevoir] ],,UDL14934,98.6842,7.80948E-45 SIF-HHPRED: Transcriptional regulator ComR; RNPP family TPR domain HTH domain bacterial signaling peptide binding, TRANSCRIPTION; 1.894A {Streptococcus vestibularis F0396},,,6HU8_A,86.8421,99.0 SIF-Syn: Downstream helix-turn-helix and upstream HP /note=earliest possible and ss CDS 16304 - 16690 /gene="24" /product="gp24" /function="Hypothetical Protein" /locus tag="GoldenBlanche_24" /note=Original Glimmer call @bp 16304 has strength 10.09; Genemark calls start at 16508 /note=SSC: 16304-16690 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein SEA_DONGWON_23 [Microbacterium phage Dongwon] ],,NCBI, q1:s1 100.0% 3.56025E-83 GAP: 509 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.655, -5.824635795707105, no F: Hypothetical Protein SIF-BLAST: ,,[hypothetical protein SEA_DONGWON_23 [Microbacterium phage Dongwon] ],,AYJ73787,100.0,3.56025E-83 SIF-HHPRED: SIF-Syn: Downstream from helix-turn-helix DNA binding upstream from helix-turn-helix DNA binding protein /note=Earliest possible and SS CDS 16774 - 16992 /gene="25" /product="gp25" /function="helix-turn-helix DNA binding domain" /locus tag="GoldenBlanche_25" /note=Original Glimmer call @bp 16774 has strength 11.99; Genemark calls start at 16783 /note=SSC: 16774-16992 CP: yes SCS: both-gl ST: SS BLAST-Start: [helix-turn-helix DNA binding domain protein [Microbacterium phage Loca]],,NCBI, q1:s1 100.0% 1.4701E-43 GAP: 83 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.206, -6.574698744401119, no F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding domain protein [Microbacterium phage Loca]],,URM86208,100.0,1.4701E-43 SIF-HHPRED: Putative DNA-binding protein; BldC, S. coelicolor, developmental switch, MerR-like, DNA BINDING PROTEIN-DNA complex; 3.09A {Streptomyces venezuelae},,,6AMA_L,76.3889,99.2 SIF-Syn: Downstream HP, upstream HNH endonuclease /note=Earliest possible and SS, Genemark start z score is much lower (but it does align with CP a bit better) CDS 16989 - 17288 /gene="26" /product="gp26" /function="HNH endonuclease" /locus tag="GoldenBlanche_26" /note=Original Glimmer call @bp 16989 has strength 7.66; Genemark calls start at 16989 /note=SSC: 16989-17288 CP: no SCS: both ST: SS BLAST-Start: [HNH endonuclease [Microbacterium phage PaoPu] ],,NCBI, q1:s1 100.0% 6.39687E-68 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.328, -6.050814105256152, no F: HNH endonuclease SIF-BLAST: ,,[HNH endonuclease [Microbacterium phage PaoPu] ],,YP_009996610,100.0,6.39687E-68 SIF-HHPRED: d.4.1.8 (A:513-673) CRISPR-associated endonuclease Cas9/Csn1, HNH domain {Actinomyces naeslundii [TaxId: 1115803]} | CLASS: Alpha and beta proteins (a+b), FOLD: His-Me finger endonucleases, SUPFAM: His-Me finger endonucleases, FAM: HNH domain from CRISPR-associated protein Cas9,,,SCOP_d4ogca2,72.7273,98.0 SIF-Syn: Downstream of helix-turn-helix DNA binding , last gene in genome /note=Earliest possible and suggested start