CDS 99 - 338 /gene="1" /product="gp1" /function="hypothetical protein" /locus tag="IsHungry_1" /note=Original Glimmer call @bp 99 has strength 14.71; Genemark calls start at 99 /note=SSC: 99-338 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_GLOBIWARMING_71 [Arthrobacter phage GlobiWarming]],,NCBI, q1:s1 92.4051% 8.32956E-40 GAP: 0 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.261, -2.033982896655645, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_GLOBIWARMING_71 [Arthrobacter phage GlobiWarming]],,UVK58463,94.5946,8.32956E-40 SIF-HHPRED: SIF-Syn: Other genes at this location also don`t have any known function CDS 328 - 672 /gene="2" /product="gp2" /function="hypothetical protein" /locus tag="IsHungry_2" /note=Original Glimmer call @bp 328 has strength 7.0; Genemark calls start at 328 /note=SSC: 328-672 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO79_gp02 [Arthrobacter phage Elesar] ],,NCBI, q1:s1 100.0% 1.81642E-28 GAP: -11 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.019, -4.912445114067465, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO79_gp02 [Arthrobacter phage Elesar] ],,YP_010761167,66.6667,1.81642E-28 SIF-HHPRED: SIF-Syn: genes in this location also typically have no known function tRNA 729 - 812 /gene="3" /product="tRNA-Ser(gct)" /locus tag="ISHUNGRY_3" /note=tRNA-Ser(gct) CDS 915 - 1313 /gene="4" /product="gp4" /function="helix-turn-helix DNA binding domain" /locus tag="IsHungry_4" /note=Original Glimmer call @bp 915 has strength 6.07; Genemark calls start at 915 /note=SSC: 915-1313 CP: yes SCS: both ST: SS BLAST-Start: [helix-turn-helix DNA binding domain protein [Arthrobacter phage Gusanita]],,NCBI, q1:s1 100.0% 3.03571E-77 GAP: 242 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.178, -4.309437458116814, no F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding domain protein [Arthrobacter phage Gusanita]],,WNM72223,94.697,3.03571E-77 SIF-HHPRED: Putative transposase for insertion sequence element IS5376; DNA transposition, DNA integration, transposon, transpososome, holo-transpososome, insertion sequence, IS21, IstA, IstB, DDE transposase, DDE domain; HET: ADP, MG, ATP;{Geobacillus stearothermophilus},,,8Q4D_D,69.697,99.0 SIF-Syn: Other genes in this location on Ichiang and GoodLuckBabe were in a similar order for the binding domain. CDS 1310 - 1948 /gene="5" /product="gp5" /function="terminase, small subunit" /locus tag="IsHungry_5" /note=Original Glimmer call @bp 1310 has strength 6.78; Genemark calls start at 1310 /note=SSC: 1310-1948 CP: yes SCS: both ST: SS BLAST-Start: [terminase small subunit [Arthrobacter phage Popper] ],,NCBI, q1:s1 94.8113% 6.68179E-123 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.165, -4.256861663406435, no F: terminase, small subunit SIF-BLAST: ,,[terminase small subunit [Arthrobacter phage Popper] ],,YP_010761099,88.2076,6.68179E-123 SIF-HHPRED: Terminase small subunit; genome packaging, bacteriophage, DNA binding, VIRAL PROTEIN; 1.4A {Enterobacteria phage HK97},,,6Z6E_A,44.8113,99.6 SIF-Syn: Other genes in this location on Ichiang and GoodLuckBabe were in a similar order for the terminase. CDS 1935 - 3632 /gene="6" /product="gp6" /function="terminase, large subunit" /locus tag="IsHungry_6" /note=Original Glimmer call @bp 1935 has strength 13.74; Genemark calls start at 1935 /note=SSC: 1935-3632 CP: yes SCS: both ST: SS BLAST-Start: [terminase large subunit [Arthrobacter phage Elesar] ],,NCBI, q1:s1 100.0% 0.0 GAP: -14 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.325, -3.9822859353357907, no F: terminase, large subunit SIF-BLAST: ,,[terminase large subunit [Arthrobacter phage Elesar] ],,YP_010761170,96.8142,0.0 SIF-HHPRED: Terminase large subunit; genome packaging, bacteriophage, ATPase, nuclease, VIRAL PROTEIN; HET: BR; 2.2A {Enterobacteria phage HK97},,,6Z6D_A,89.0266,100.0 SIF-Syn: Large terminase subunit, aligns closely with Gusanita, Elesar, Cole. CDS 3648 - 4928 /gene="7" /product="gp7" /function="portal protein" /locus tag="IsHungry_7" /note=Original Glimmer call @bp 3648 has strength 12.83; Genemark calls start at 3648 /note=SSC: 3648-4928 CP: yes SCS: both ST: SS BLAST-Start: [portal protein [Arthrobacter phage Cole] ],,NCBI, q1:s1 100.0% 0.0 GAP: 15 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.261, -1.953940808934884, yes F: portal protein SIF-BLAST: ,,[portal protein [Arthrobacter phage Cole] ],,YP_010760971,98.8263,0.0 SIF-HHPRED: Portal protein; G20C, portal protein, bacteriophage, transport protein; 1.9A {Thermus phage P7426},,,5NGD_D,96.4789,100.0 SIF-Syn: Other phages had similar conservation of order of genes for portal proteins at this locations (Ryan and Ichiang) CDS 4925 - 6178 /gene="8" /product="gp8" /function="capsid maturation protease" /locus tag="IsHungry_8" /note=Original Glimmer call @bp 4976 has strength 11.28; Genemark calls start at 4937 /note=SSC: 4925-6178 CP: yes SCS: both-cs ST: SS BLAST-Start: [capsid maturation protease [Arthrobacter phage Gusanita]],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.711, -3.188585239877588, no F: capsid maturation protease SIF-BLAST: ,,[capsid maturation protease [Arthrobacter phage Gusanita]],,WNM72227,97.6019,0.0 SIF-HHPRED: Putative ATP-dependent Clp protease proteolytic subunit; mitochondrial ClpP, Clp/Hsp 100, ATP-dependent protease, HYDROLASE; HET: DIO, FME, EDO, GOL; 2.1A {Homo sapiens} SCOP: c.14.1.1,,,1TG6_G,38.8489,99.8 SIF-Syn: Capsid maturation protease, aligns closely with Gusanita, Elesar, Cole. CDS 6181 - 6543 /gene="9" /product="gp9" /function="scaffolding protein" /locus tag="IsHungry_9" /note=Original Glimmer call @bp 6181 has strength 10.66; Genemark calls start at 6181 /note=SSC: 6181-6543 CP: yes SCS: both ST: SS BLAST-Start: [scaffolding protein [Arthrobacter phage Nandita] ],,NCBI, q1:s1 100.0% 1.60352E-73 GAP: 2 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.521, -3.649482460397077, yes F: scaffolding protein SIF-BLAST: ,,[scaffolding protein [Arthrobacter phage Nandita] ],,YP_010760838,99.1667,1.60352E-73 SIF-HHPRED: Capsid stabilization protein; Archaeal virus, portal, portal capsid interface, Mg ions, VIRUS; HET: MG, HIP, K; 2.68A {Haloferax tailed virus 1},,,8QSY_KI,99.1667,99.8 SIF-Syn: Aligns closely with Nandita, Popper, Ryan, and other genes CDS 6554 - 7483 /gene="10" /product="gp10" /function="major capsid protein" /locus tag="IsHungry_10" /note=Original Glimmer call @bp 6554 has strength 16.09; Genemark calls start at 6554 /note=SSC: 6554-7483 CP: yes SCS: both ST: SS BLAST-Start: [major capsid protein [Arthrobacter phage Gusanita]],,NCBI, q1:s1 100.0% 0.0 GAP: 10 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.272, -1.9310779259753799, yes F: major capsid protein SIF-BLAST: ,,[major capsid protein [Arthrobacter phage Gusanita]],,WNM72229,99.3528,0.0 SIF-HHPRED: MAJOR CAPSID PROTEIN; VIRUS, VIRUS COAT PROTEIN, VIRUS/VIRAL PROTEIN, BACTERIOPHAGE, CAPSID, AUTO- CATALYTIC CROSS-LINK, ICOSAHEDRAL VIRUS; 3.45A {BACTERIOPHAGE HK97} SCOP: d.183.1.1,,,1OHG_B,90.6149,99.9 SIF-Syn: Compared to Nandita, very well conserved. CDS 7485 - 7712 /gene="11" /product="gp11" /function="hypothetical protein" /locus tag="IsHungry_11" /note=Original Glimmer call @bp 7485 has strength 11.1; Genemark calls start at 7485 /note=SSC: 7485-7712 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_GUSANITA_9 [Arthrobacter phage Gusanita]],,NCBI, q1:s1 100.0% 8.86254E-40 GAP: 1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.167, -4.252258253355557, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_GUSANITA_9 [Arthrobacter phage Gusanita]],,WNM72230,93.3333,8.86254E-40 SIF-HHPRED: GINS_B_archaea_Gins23; beta-strand (B) domain of archaeal GINS complex protein Gins23. The GINS (named from the Japanese go-ichi-ni-san, meaning 5-1-2-3 for the Sld5, Psf1, Psf2, and Psf3 subunits) complex is involved in both initiation and elongation stages of eukaryotic chromosome replication, with GINS being the component that most likely serves as the replicative helicase that unwinds duplex DNA ahead of the moving replication fork.,,,cd21697,38.6667,92.5 SIF-Syn: No known function assigned on maps. CDS 7727 - 8128 /gene="12" /product="gp12" /function="head-to-tail adaptor" /locus tag="IsHungry_12" /note=Original Glimmer call @bp 7727 has strength 7.15; Genemark calls start at 7727 /note=SSC: 7727-8128 CP: yes SCS: both ST: SS BLAST-Start: [head-to-tail adaptor [Arthrobacter phage Popper] ],,NCBI, q1:s1 99.2481% 4.75907E-87 GAP: 14 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.029, -2.5052746077145835, yes F: head-to-tail adaptor SIF-BLAST: ,,[head-to-tail adaptor [Arthrobacter phage Popper] ],,YP_010761106,98.4962,4.75907E-87 SIF-HHPRED: Head-to-tail adaptor; Bacteriophage, portal, VIRAL PROTEIN;{Mycobacterium phage Bxb1},,,9D94_Ga,93.985,99.9 SIF-Syn: Aligns with Popper, Nandita, and Elesar, which all indicate the gene`s function as head-to-tail adaptor. CDS 8131 - 8463 /gene="13" /product="gp13" /function="head-to-tail stopper" /locus tag="IsHungry_13" /note=Original Glimmer call @bp 8122 has strength 8.04; Genemark calls start at 8131 /note=SSC: 8131-8463 CP: yes SCS: both-gm ST: NI BLAST-Start: [head-to-tail stopper [Arthrobacter phage Gusanita]],,NCBI, q1:s1 100.0% 2.20396E-66 GAP: 2 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.501, -4.202820482200625, yes F: head-to-tail stopper SIF-BLAST: ,,[head-to-tail stopper [Arthrobacter phage Gusanita]],,WNM72232,95.4545,2.20396E-66 SIF-HHPRED: DUF3599 ; Domain of unknown function (DUF3599),,,PF12206.13,94.5455,99.6 SIF-Syn: BLAST and Phamerator indicate two different functions, while HHPred shows a high probability that there is no gene function. No known function is indicated due to contradicting evidence. CDS 8460 - 8735 /gene="14" /product="gp14" /function="hypothetical protein" /locus tag="IsHungry_14" /note=Original Glimmer call @bp 8460 has strength 8.73; Genemark calls start at 8460 /note=SSC: 8460-8735 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_GUSANITA_12 [Arthrobacter phage Gusanita]],,NCBI, q1:s1 100.0% 1.60307E-54 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.275, -4.165057350296871, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_GUSANITA_12 [Arthrobacter phage Gusanita]],,WNM72233,95.6044,1.60307E-54 SIF-HHPRED: DUF5403 ; Family of unknown function (DUF5403),,,PF17395.7,76.9231,93.2 SIF-Syn: BLAST and HHPred indicate no known gene function. CDS 8732 - 9145 /gene="15" /product="gp15" /function="tail terminator" /locus tag="IsHungry_15" /note=Original Glimmer call @bp 8732 has strength 14.49; Genemark calls start at 8732 /note=SSC: 8732-9145 CP: yes SCS: both ST: SS BLAST-Start: [tail terminator [Arthrobacter phage Gusanita]],,NCBI, q1:s1 100.0% 1.16333E-90 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.022, -3.284137222879636, yes F: tail terminator SIF-BLAST: ,,[tail terminator [Arthrobacter phage Gusanita]],,WNM72234,98.5401,1.16333E-90 SIF-HHPRED: Tail terminator; Bacteriophage, portal, VIRAL PROTEIN;{Mycobacterium phage Bxb1},,,9D94_Ic,96.3504,99.9 SIF-Syn: Gene aligns with that of Popper, Nandita, and Elesar`s, all of which have the head-to-tail terminator function. CDS 9202 - 9870 /gene="16" /product="gp16" /function="major tail protein" /locus tag="IsHungry_16" /note=Original Glimmer call @bp 9202 has strength 18.1; Genemark calls start at 9202 /note=SSC: 9202-9870 CP: yes SCS: both ST: SS BLAST-Start: [major tail protein [Arthrobacter phage Gusanita]],,NCBI, q1:s1 100.0% 2.23436E-156 GAP: 56 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.85, -3.1681521309447884, no F: major tail protein SIF-BLAST: ,,[major tail protein [Arthrobacter phage Gusanita]],,WNM72235,100.0,2.23436E-156 SIF-HHPRED: Major tail protein; Bacteriophage, tail tube, VIRUS, VIRAL PROTEIN;{Mycobacterium phage Bxb1},,,9D9L_J,88.2883,99.9 SIF-Syn: Compared to Guasinita and Ryan, very well conserved CDS 9961 - 10125 /gene="17" /product="gp17" /function="hypothetical protein" /locus tag="IsHungry_17" /note=Original Glimmer call @bp 9961 has strength 16.38; Genemark calls start at 9961 /note=SSC: 9961-10125 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO78_gp15 [Arthrobacter phage Popper] ],,NCBI, q1:s1 98.1481% 2.02636E-18 GAP: 90 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.365, -3.8366550365551095, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO78_gp15 [Arthrobacter phage Popper] ],,YP_010761111,87.037,2.02636E-18 SIF-HHPRED: SIF-Syn: Compared to Guasinita and Ryan, very well conserved CDS 10156 - 10494 /gene="18" /product="gp18" /function="tail assembly chaperone" /locus tag="IsHungry_18" /note=Original Glimmer call @bp 10156 has strength 15.94; Genemark calls start at 10156 /note=SSC: 10156-10494 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO74_gp51 [Arthrobacter phage Nandita] ],,NCBI, q1:s1 100.0% 2.51183E-70 GAP: 30 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.403, -3.8970005018578204, yes F: tail assembly chaperone SIF-BLAST: ,,[hypothetical protein QEO74_gp51 [Arthrobacter phage Nandita] ],,YP_010760848,96.4286,2.51183E-70 SIF-HHPRED: GP24_25 ; Mycobacteriophage tail assembly protein,,,PF17388.7,82.1429,98.4 SIF-Syn: Compared to Guasinita and Ryan, very well conserved CDS join(10156..10470,10470..10931) /gene="19" /product="gp19" /function="tail assembly chaperone" /locus tag="IsHungry_19" /note= /note=SSC: 10156-10931 CP: no SCS: neither ST: SS BLAST-Start: GAP: -339 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.403, -3.8970005018578204, yes F: tail assembly chaperone SIF-BLAST: SIF-HHPRED: GP24_25 ; Mycobacteriophage tail assembly protein,,,PF17388.7,18.9922,98.5 SIF-Syn: Nandita, Zaheer, Cole, Ryan, Gusanita, and QuinnAvery all have two tail assembly chaperones, one longer and one shorter, between a short NKF gene and a long tape measure protein. CDS 10951 - 15765 /gene="20" /product="gp20" /function="tape measure protein" /locus tag="IsHungry_20" /note=Original Glimmer call @bp 10951 has strength 9.65; Genemark calls start at 10951 /note=SSC: 10951-15765 CP: yes SCS: both ST: SS BLAST-Start: [tape measure protein [Arthrobacter phage Cole] ],,NCBI, q1:s1 100.0% 0.0 GAP: 18 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.591, -3.441249337670496, no F: tape measure protein SIF-BLAST: ,,[tape measure protein [Arthrobacter phage Cole] ],,YP_010760984,95.0125,0.0 SIF-HHPRED: Tape Measure Protein, gp57; phage tail, tail tip, tape measure protein, VIRAL PROTEIN; 3.7A {Staphylococcus virus 80alpha},,,6V8I_BF,10.2868,99.9 SIF-Syn: Compared to Guasinita and Ryan, very well conserved CDS 15775 - 16695 /gene="21" /product="gp21" /function="minor tail protein" /locus tag="IsHungry_21" /note=Original Glimmer call @bp 15775 has strength 13.11; Genemark calls start at 15775 /note=SSC: 15775-16695 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Arthrobacter phage Gusanita]],,NCBI, q1:s1 100.0% 0.0 GAP: 9 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.132, -4.325675514574479, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage Gusanita]],,WNM72240,98.366,0.0 SIF-HHPRED: HYPOTHETICAL PROTEIN 19.1; VIRAL PROTEIN, DISTAL TAIL PROTEIN; 2.95A {BACILLUS PHAGE SPP1},,,2X8K_A,93.7908,100.0 SIF-Syn: minor tail protein also in this location after tape measure protein in closely related phage CDS 16699 - 17757 /gene="22" /product="gp22" /function="minor tail protein" /locus tag="IsHungry_22" /note=Original Glimmer call @bp 16699 has strength 10.62; Genemark calls start at 16699 /note=SSC: 16699-17757 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Arthrobacter phage Gusanita]],,NCBI, q1:s1 100.0% 0.0 GAP: 3 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.71, -5.27259541663274, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage Gusanita]],,WNM72241,96.0227,0.0 SIF-HHPRED: Receptor Binding Protein; beta sandwich domain, phage receptor binding protein, Lactococcus lactis pellicle cell wall polyphosphosaccharide, VIRAL PROTEIN; 1.75A {Lactococcus phage 1358},,,4L9B_A,43.1818,98.7 SIF-Syn: minor tail protein also in this location after tape measure protein in closely related phage CDS 17775 - 18092 /gene="23" /product="gp23" /function="membrane protein" /locus tag="IsHungry_23" /note=Original Glimmer call @bp 17775 has strength 9.49; Genemark calls start at 17775 /note=SSC: 17775-18092 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO75_gp23 [Arthrobacter phage Ryan] ],,NCBI, q1:s1 100.0% 2.78578E-55 GAP: 17 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.779, -3.0301037898918786, yes F: membrane protein SIF-BLAST: ,,[hypothetical protein QEO75_gp23 [Arthrobacter phage Ryan] ],,YP_010760919,89.6226,2.78578E-55 SIF-HHPRED: SIF-Syn: Compared to Guasinita and Ryan, very well conserved CDS 18089 - 19024 /gene="24" /product="gp24" /function="membrane protein" /locus tag="IsHungry_24" /note=Original Glimmer call @bp 18089 has strength 3.16; Genemark calls start at 18089 /note=SSC: 18089-19024 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Arthrobacter phage Ryan] ],,NCBI, q2:s6 97.4277% 8.88454E-140 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.875, -2.9063687850157054, yes F: membrane protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage Ryan] ],,YP_010760920,79.288,8.88454E-140 SIF-HHPRED: SIF-Syn: Compared to Guasinita and Ryan, appears to be an NKF region CDS 19021 - 19965 /gene="25" /product="gp25" /function="endolysin" /locus tag="IsHungry_25" /note=Original Glimmer call @bp 19021 has strength 13.18; Genemark calls start at 19021 /note=SSC: 19021-19965 CP: yes SCS: both ST: SS BLAST-Start: [endolysin [Arthrobacter phage BruhMoment]],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.857, -4.109171212198345, no F: endolysin SIF-BLAST: ,,[endolysin [Arthrobacter phage BruhMoment]],,UOK18342,92.283,0.0 SIF-HHPRED: Peptidase M23; membrane protein, enzyme, TRANSPORT PROTEIN; HET: ADP;{Vibrio cholerae},,,8TZL_E,36.3057,99.2 SIF-Syn: Compared to Guasinita and Ryan, very well conserved. /note=BLASTs to peptidase domain but doesn`t seem to have a lysin B so per phage function list function is endolysin. CDS 19976 - 20278 /gene="26" /product="gp26" /function="membrane protein" /locus tag="IsHungry_26" /note=Original Glimmer call @bp 19976 has strength 13.36; Genemark calls start at 19976 /note=SSC: 19976-20278 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein [Arthrobacter sp.]],,NCBI, q1:s1 100.0% 5.43171E-60 GAP: 10 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.176, -2.13349812375341, yes F: membrane protein SIF-BLAST: ,,[hypothetical protein [Arthrobacter sp.]],,HEY9356480,97.0297,5.43171E-60 SIF-HHPRED: SIF-Syn: Compared to Guasinita and Ryan, very well conserved CDS 20288 - 21391 /gene="27" /product="gp27" /function="minor tail protein" /locus tag="IsHungry_27" /note=Original Glimmer call @bp 20288 has strength 16.11; Genemark calls start at 20288 /note=SSC: 20288-21391 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Arthrobacter phage Gusanita]],,NCBI, q1:s1 100.0% 0.0 GAP: 9 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.132, -4.325675514574479, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage Gusanita]],,WNM72246,97.8202,0.0 SIF-HHPRED: Minor tail protein; Bacteriophage, tail tip, VIRAL PROTEIN;{Mycobacterium phage Bxb1},,,9D93_Oa,98.0926,99.8 SIF-Syn: Compared to Guasinita and Ryan, very well conserved CDS 21392 - 22369 /gene="28" /product="gp28" /function="minor tail protein" /locus tag="IsHungry_28" /note=Original Glimmer call @bp 21392 has strength 15.06; Genemark calls start at 21392 /note=SSC: 21392-22369 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Arthrobacter phage Gusanita]],,NCBI, q4:s3 99.0769% 0.0 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.932, -2.7863799983944713, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage Gusanita]],,WNM72247,97.4763,0.0 SIF-HHPRED: Putative tail fiber protein; Complex, VIRAL PROTEIN; 3.8A {Ralstonia phage GP4},,,8JOV_M,35.3846,96.7 SIF-Syn: When compared to other phages in the same FF family in phamorator, there is a solid purple color with Nandita and Gusanita, and they both called minor tail protein CDS 22369 - 22728 /gene="29" /product="gp29" /function="hypothetical protein" /locus tag="IsHungry_29" /note=Original Glimmer call @bp 22369 has strength 10.91; Genemark calls start at 22369 /note=SSC: 22369-22728 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_GUSANITA_27 [Arthrobacter phage Gusanita]],,NCBI, q1:s1 100.0% 8.69782E-74 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.367, -3.8932062518944837, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_GUSANITA_27 [Arthrobacter phage Gusanita]],,WNM72248,96.6387,8.69782E-74 SIF-HHPRED: SIF-Syn: Compared to Guasinita and Ryan, this region is typically NKF CDS 22738 - 23466 /gene="30" /product="gp30" /function="hypothetical protein" /locus tag="IsHungry_30" /note=Original Glimmer call @bp 22738 has strength 14.95; Genemark calls start at 22738 /note=SSC: 22738-23466 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO76_gp28 [Arthrobacter phage Cole] ],,NCBI, q1:s1 100.0% 4.19944E-162 GAP: 9 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.132, -4.325675514574479, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO76_gp28 [Arthrobacter phage Cole] ],,YP_010760994,96.281,4.19944E-162 SIF-HHPRED: SIF-Syn: quinn avery had a purple background and called it minor tail protein CDS 23474 - 24847 /gene="31" /product="gp31" /function="glucosaminyl deacetylase" /locus tag="IsHungry_31" /note=Original Glimmer call @bp 23474 has strength 13.04; Genemark calls start at 23474 /note=SSC: 23474-24847 CP: yes SCS: both ST: SS BLAST-Start: [polysaccharide deacetylase family protein [Streptomyces sp. NBC_00830]],,NCBI, q5:s106 98.2495% 3.90551E-61 GAP: 7 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.132, -4.85287563363746, no F: glucosaminyl deacetylase SIF-BLAST: ,,[polysaccharide deacetylase family protein [Streptomyces sp. NBC_00830]],,WTB31180,42.0608,3.90551E-61 SIF-HHPRED: Prokaryotic polysaccharide deacetylase; Archeal virus, turret, turret capsid interface, Mg ions, VIRUS; HET: MG, NEP; 2.36A {Haloferax tailed virus 1},,,8QPG_TA,85.9956,100.0 SIF-Syn: Compared to Guasinita and Ryan, very well conserved CDS 25028 - 25948 /gene="32" /product="gp32" /function="tyrosine integrase" /locus tag="IsHungry_32" /note=Original Glimmer call @bp 25028 has strength 13.68; Genemark calls start at 25028 /note=SSC: 25028-25948 CP: yes SCS: both ST: SS BLAST-Start: [tyrosine-type recombinase/integrase [Arthrobacter phage Maja] ],,NCBI, q1:s1 100.0% 0.0 GAP: 180 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.607, -3.4076099559729456, yes F: tyrosine integrase SIF-BLAST: ,,[tyrosine-type recombinase/integrase [Arthrobacter phage Maja] ],,YP_009818588,97.0588,0.0 SIF-HHPRED: Int protein; transposase protein-DNA complex, tyrosine recombinase, Y-transposase, Tn916-like conjugative transposon, antibiotic resistance transfer, RECOMBINATION; 2.5A {Enterococcus faecalis},,,6EMY_B,92.8105,100.0 SIF-Syn: Conserved in Guasinita but tyrosine integrate is a little farther down the genome on phage Ryan tRNA 25995 - 26068 /gene="33" /product="tRNA-Arg(tct)" /locus tag="ISHUNGRY_33" /note=tRNA-Arg(tct) CDS complement (26117 - 26992) /gene="34" /product="gp34" /function="tyrosine integrase" /locus tag="IsHungry_34" /note=Original Glimmer call @bp 26992 has strength 11.46; Genemark calls start at 26992 /note=SSC: 26992-26117 CP: yes SCS: both ST: SS BLAST-Start: [integrase [Arthrobacter phage Nandita] ],,NCBI, q1:s1 100.0% 0.0 GAP: 711 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.121, -4.349108688274226, no F: tyrosine integrase SIF-BLAST: ,,[integrase [Arthrobacter phage Nandita] ],,YP_010760867,98.6254,0.0 SIF-HHPRED: Gp33; Bacteriophage, Brujita, DNA-binding, Integrase, DNA BINDING PROTEIN; HET: ACT, GOL; 1.847A {Mycobacterium phage Brujita},,,5VFZ_A,96.5636,100.0 SIF-Syn: labels as just integrase but generally conserved in phages Guanisita and Ryan CDS complement (27704 - 27835) /gene="35" /product="gp35" /function="hypothetical protein" /locus tag="IsHungry_35" /note=Original Glimmer call @bp 27835 has strength 2.48 /note=SSC: 27835-27704 CP: yes SCS: glimmer ST: NI BLAST-Start: [hypothetical protein QEO77_gp65 [Arthrobacter phage Zaheer] ],,NCBI, q4:s8 93.0233% 1.26334E-12 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.099, -4.4747668481608205, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO77_gp65 [Arthrobacter phage Zaheer] ],,YP_010761069,72.3404,1.26334E-12 SIF-HHPRED: SIF-Syn: not marked as a gene on phages Guasanita or Ryan CDS complement (27832 - 28296) /gene="36" /product="gp36" /function="hypothetical protein" /locus tag="IsHungry_36" /note=Original Glimmer call @bp 28296 has strength 5.05; Genemark calls start at 28296 /note=SSC: 28296-27832 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_GUSANITA_39 [Arthrobacter phage Gusanita]],,NCBI, q1:s1 98.7013% 2.43314E-83 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.532, -3.627004739933808, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_GUSANITA_39 [Arthrobacter phage Gusanita]],,WNM72259,85.6209,2.43314E-83 SIF-HHPRED: SIF-Syn: NKF on phages Guasanita and Ryan CDS complement (28293 - 28697) /gene="37" /product="gp37" /function="helix-turn-helix DNA binding domain" /locus tag="IsHungry_37" /note=Original Glimmer call @bp 28541 has strength 0.44; Genemark calls start at 28697 /note=SSC: 28697-28293 CP: yes SCS: both-gm ST: SS BLAST-Start: [helix-turn-helix DNA binding domain protein [Arthrobacter phage Gusanita]],,NCBI, q1:s1 100.0% 3.02042E-73 GAP: 133 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.581, -6.58298292630672, no F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding domain protein [Arthrobacter phage Gusanita]],,WNM72260,89.6296,3.02042E-73 SIF-HHPRED: ComR; Streptococcus, Competence, Quorum sensing, ComR, TRANSCRIPTION REGULATOR; 2.9A {Streptococcus suis (strain 05ZYH33)},,,5FD4_B,50.0,98.1 SIF-Syn: Compared to Guasinita and Ryan, very well conserved CDS 28831 - 29040 /gene="38" /product="gp38" /function="helix-turn-helix DNA binding domain" /locus tag="IsHungry_38" /note=Original Glimmer call @bp 28831 has strength 1.59; Genemark calls start at 28831 /note=SSC: 28831-29040 CP: yes SCS: both ST: SS BLAST-Start: [helix-turn-helix DNA binding domain protein [Arthrobacter phage Gusanita]],,NCBI, q1:s1 100.0% 1.98977E-39 GAP: 133 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 0.901, -7.560645402102623, yes F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding domain protein [Arthrobacter phage Gusanita]],,WNM72261,97.1014,1.98977E-39 SIF-HHPRED: AlpA family phage regulatory protein; excisionase, mobile genetic elements, recombination, DNA BINDING PROTEIN; 2.11A {Achromobacter xylosoxidans NBRC 15126 = ATCC 27061},,,8C3T_A,94.2029,99.2 SIF-Syn: Compared to Guasinita and Ryan, very well conserved CDS 29178 - 29774 /gene="39" /product="gp39" /function="hypothetical protein" /locus tag="IsHungry_39" /note=Original Glimmer call @bp 29178 has strength 8.05; Genemark calls start at 29178 /note=SSC: 29178-29774 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO78_gp63 [Arthrobacter phage Popper] ],,NCBI, q1:s1 100.0% 2.99704E-126 GAP: 137 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.7, -5.293557664252809, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO78_gp63 [Arthrobacter phage Popper] ],,YP_010761138,90.2439,2.99704E-126 SIF-HHPRED: SIF-Syn: conserved compared to nandita and popper, purple coloring seen between gene 37 and gene 45 and 43 respectively. no specificed function on phamerator CDS 29771 - 30028 /gene="40" /product="gp40" /function="hypothetical protein" /locus tag="IsHungry_40" /note=Original Glimmer call @bp 29771 has strength 13.08; Genemark calls start at 29771 /note=SSC: 29771-30028 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO79_gp56 [Arthrobacter phage Elesar] ],,NCBI, q1:s1 100.0% 9.67156E-52 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.019, -4.702334767722353, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO79_gp56 [Arthrobacter phage Elesar] ],,YP_010761204,95.2941,9.67156E-52 SIF-HHPRED: SIF-Syn: nandita and gusantia, high purple coloring. phamerator in hhpred also said protein transport CDS 30025 - 30219 /gene="41" /product="gp41" /function="membrane protein" /locus tag="IsHungry_41" /note=Original Glimmer call @bp 30025 has strength 8.33; Genemark calls start at 30025 /note=SSC: 30025-30219 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO77_gp59 [Arthrobacter phage Zaheer] ],,NCBI, q1:s1 100.0% 1.16214E-23 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.953, -2.66371296573402, yes F: membrane protein SIF-BLAST: ,,[hypothetical protein QEO77_gp59 [Arthrobacter phage Zaheer] ],,YP_010761075,93.75,1.16214E-23 SIF-HHPRED: SIF-Syn: phamerator in hhpred also said membrane protein CDS 30212 - 30748 /gene="42" /product="gp42" /function="hypothetical protein" /locus tag="IsHungry_42" /note=Original Glimmer call @bp 30212 has strength 7.84; Genemark calls start at 30212 /note=SSC: 30212-30748 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO76_gp56 [Arthrobacter phage Cole] ],,NCBI, q1:s1 100.0% 2.01288E-119 GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.09, -2.314799364732351, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO76_gp56 [Arthrobacter phage Cole] ],,YP_010761009,97.7528,2.01288E-119 SIF-HHPRED: SIF-Syn: no known function on maps CDS 30745 - 32109 /gene="43" /product="gp43" /function="ParB-like dsDNA partitioning protein" /locus tag="IsHungry_43" /note=Original Glimmer call @bp 30745 has strength 12.71; Genemark calls start at 30745 /note=SSC: 30745-32109 CP: yes SCS: both ST: SS BLAST-Start: [ParB-like DNA binding protein [Arthrobacter phage Popper] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.403, -3.8970005018578204, no F: ParB-like dsDNA partitioning protein SIF-BLAST: ,,[ParB-like DNA binding protein [Arthrobacter phage Popper] ],,YP_010761142,97.3568,0.0 SIF-HHPRED: ParB family protein; DNA-binding protein, CTP, Myxococcus, DNA-segregation, DNA BINDING PROTEIN; HET: UFQ, GOL; 1.7A {Myxococcus xanthus (strain DK 1622)},,,7BNR_B,41.63,99.8 SIF-Syn: matches nadita length and function as a DNA binding protein likely a DNA binding protein CDS 32102 - 32494 /gene="44" /product="gp44" /function="hypothetical protein" /locus tag="IsHungry_44" /note=Original Glimmer call @bp 32102 has strength 9.71; Genemark calls start at 32102 /note=SSC: 32102-32494 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO77_gp56 [Arthrobacter phage Zaheer] ],,NCBI, q1:s1 100.0% 1.92419E-70 GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.186, -2.17469248771465, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO77_gp56 [Arthrobacter phage Zaheer] ],,YP_010761078,82.2222,1.92419E-70 SIF-HHPRED: SIF-Syn: calls a structural protein 80% possible protein (need more info) CDS 32609 - 33034 /gene="45" /product="gp45" /function="hypothetical protein" /locus tag="IsHungry_45" /note=Original Glimmer call @bp 32609 has strength 4.46; Genemark calls start at 32603 /note=SSC: 32609-33034 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein SEA_GUSANITA_49 [Arthrobacter phage Gusanita]],,NCBI, q2:s1 99.2908% 1.18275E-72 GAP: 114 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.734, -3.061661148509975, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_GUSANITA_49 [Arthrobacter phage Gusanita]],,WNM72269,87.7551,1.18275E-72 SIF-HHPRED: SIF-Syn: no known function on maps tRNA 33062 - 33137 /gene="46" /product="tRNA-Gly(ccc)" /locus tag="ISHUNGRY_46" /note=tRNA-Gly(ccc) CDS 33169 - 33972 /gene="47" /product="gp47" /function="helix-turn-helix DNA binding domain" /locus tag="IsHungry_47" /note=Original Glimmer call @bp 33169 has strength 8.21; Genemark calls start at 33169 /note=SSC: 33169-33972 CP: yes SCS: both ST: SS BLAST-Start: [helix-turn-helix DNA-binding domain protein [Arthrobacter phage Popper] ],,NCBI, q1:s1 100.0% 0.0 GAP: 134 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.261, -1.953940808934884, yes F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA-binding domain protein [Arthrobacter phage Popper] ],,YP_010761147,98.5019,0.0 SIF-HHPRED: CAP REPEAT; diffusion, generation, de novo, RoseTTAFold, sequence, ProteinGenerator, PROTEIN GENERATOR, deep learning, machine learning, generative model, DE; 3.7A {synthetic construct},,,8VD6_A,83.1461,98.7 SIF-Syn: no known function supported by the map CDS 33986 - 34165 /gene="48" /product="gp48" /function="hypothetical protein" /locus tag="IsHungry_48" /note=Original Glimmer call @bp 33986 has strength 3.54 /note=SSC: 33986-34165 CP: yes SCS: glimmer ST: SS BLAST-Start: [hypothetical protein QEO78_gp53 [Arthrobacter phage Popper] ],,NCBI, q1:s1 100.0% 1.31492E-33 GAP: 13 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.272, -2.2821867859826788, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO78_gp53 [Arthrobacter phage Popper] ],,YP_010761148,96.6102,1.31492E-33 SIF-HHPRED: SIF-Syn: No known function supported by map CDS 34162 - 35040 /gene="49" /product="gp49" /function="DNA polymerase III sliding clamp (Beta)" /locus tag="IsHungry_49" /note=Original Glimmer call @bp 34162 has strength 13.36; Genemark calls start at 34162 /note=SSC: 34162-35040 CP: yes SCS: both ST: SS BLAST-Start: [DNA polymerase III sliding clamp beta [Arthrobacter phage Popper] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.122, -4.34663776219455, no F: DNA polymerase III sliding clamp (Beta) SIF-BLAST: ,,[DNA polymerase III sliding clamp beta [Arthrobacter phage Popper] ],,YP_010761149,99.3127,0.0 SIF-HHPRED: Beta sliding clamp; gp168, DNA beta-clamp, cross-species inhibitor, ANTIMICROBIAL PROTEIN;{Staphylococcus aureus},,,7EVP_B,70.2055,99.8 SIF-Syn: Calls for function supporting DNA polymerase III CDS 35037 - 35465 /gene="50" /product="gp50" /function="hypothetical protein" /locus tag="IsHungry_50" /note=Original Glimmer call @bp 35037 has strength 6.41; Genemark calls start at 35037 /note=SSC: 35037-35465 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO78_gp51 [Arthrobacter phage Popper] ],,NCBI, q1:s1 100.0% 1.25246E-97 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.454, -3.790975366313636, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO78_gp51 [Arthrobacter phage Popper] ],,YP_010761150,97.1831,1.25246E-97 SIF-HHPRED: SIF-Syn: No known function shown in map CDS 35462 - 35677 /gene="51" /product="gp51" /function="hypothetical protein" /locus tag="IsHungry_51" /note=Original Glimmer call @bp 35462 has strength 8.17; Genemark calls start at 35462 /note=SSC: 35462-35677 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO78_gp50 [Arthrobacter phage Popper] ],,NCBI, q1:s1 100.0% 9.74779E-42 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.735, -5.238109312333945, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO78_gp50 [Arthrobacter phage Popper] ],,YP_010761151,98.5916,9.74779E-42 SIF-HHPRED: SIF-Syn: No known function shown on map CDS 35674 - 35874 /gene="52" /product="gp52" /function="hypothetical protein" /locus tag="IsHungry_52" /note=Original Glimmer call @bp 35674 has strength 8.42; Genemark calls start at 35674 /note=SSC: 35674-35874 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO78_gp49 [Arthrobacter phage Popper] ],,NCBI, q1:s1 96.9697% 3.35997E-37 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.932, -2.707694805492614, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO78_gp49 [Arthrobacter phage Popper] ],,YP_010761152,95.4545,3.35997E-37 SIF-HHPRED: SIF-Syn: nkf on gusanita and ryan CDS 35871 - 36029 /gene="53" /product="gp53" /function="hypothetical protein" /locus tag="IsHungry_53" /note=Original Glimmer call @bp 35871 has strength 3.78; Genemark calls start at 35871 /note=SSC: 35871-36029 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO77_gp48 [Arthrobacter phage Zaheer] ],,NCBI, q1:s1 98.0769% 4.61904E-25 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.019, -4.6236495748204955, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO77_gp48 [Arthrobacter phage Zaheer] ],,YP_010761086,94.2308,4.61904E-25 SIF-HHPRED: SIF-Syn: nkf on gusanita and Ryan CDS 36026 - 36223 /gene="54" /product="gp54" /function="membrane protein" /locus tag="IsHungry_54" /note=Original Glimmer call @bp 36026 has strength 6.82; Genemark calls start at 36026 /note=SSC: 36026-36223 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO78_gp47 [Arthrobacter phage Popper] ],,NCBI, q1:s1 100.0% 3.6319E-14 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.122, -4.34663776219455, no F: membrane protein SIF-BLAST: ,,[hypothetical protein QEO78_gp47 [Arthrobacter phage Popper] ],,YP_010761154,87.6923,3.6319E-14 SIF-HHPRED: SIF-Syn: Highly conserved, compared with Popper, Gusanita, and Zaheer. CDS 36216 - 36395 /gene="55" /product="gp55" /function="hypothetical protein" /locus tag="IsHungry_55" /note=Original Glimmer call @bp 36216 has strength 3.44; Genemark calls start at 36216 /note=SSC: 36216-36395 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO78_gp46 [Arthrobacter phage Popper] ],,NCBI, q1:s1 100.0% 4.7176E-22 GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.542, -3.4645021069762962, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO78_gp46 [Arthrobacter phage Popper] ],,YP_010761155,96.6102,4.7176E-22 SIF-HHPRED: SIF-Syn: No known function assigned on maps. CDS 36395 - 36580 /gene="56" /product="gp56" /function="hypothetical protein" /locus tag="IsHungry_56" /note=Original Glimmer call @bp 36395 has strength 13.41; Genemark calls start at 36395 /note=SSC: 36395-36580 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO78_gp45 [Arthrobacter phage Popper] ],,NCBI, q1:s1 100.0% 1.2378E-33 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.137, -4.9668027763900575, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO78_gp45 [Arthrobacter phage Popper] ],,YP_010761156,98.3607,1.2378E-33 SIF-HHPRED: d.222.1.0 (A:1-92) automated matches {Helicobacter pylori [TaxId: 210]} | CLASS: Alpha and beta proteins (a+b), FOLD: YbaB-like, SUPFAM: YbaB-like, FAM: automated matches,,,SCOP_d3f42a1,62.2951,91.6 SIF-Syn: No known function assigned on maps. CDS 36577 - 36846 /gene="57" /product="gp57" /function="helix-turn-helix DNA binding domain" /locus tag="IsHungry_57" /note=Original Glimmer call @bp 36577 has strength 13.3; Genemark calls start at 36577 /note=SSC: 36577-36846 CP: yes SCS: both ST: NI BLAST-Start: [helix-turn-helix DNA-binding domain protein [Arthrobacter phage Popper] ],,NCBI, q1:s1 98.8764% 1.97006E-40 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.614, -5.4121604219751855, no F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA-binding domain protein [Arthrobacter phage Popper] ],,YP_010761157,91.0112,1.97006E-40 SIF-HHPRED: Middle operon regulator; Mor, TRANSCRIPTION; 2.2A {Enterobacteria phage Mu} SCOP: a.4.1.14,,,1RR7_A,91.0112,94.8 SIF-Syn: Compared with Nandita and Popper, highly conserved. CDS 36843 - 37187 /gene="58" /product="gp58" /function="hypothetical protein" /locus tag="IsHungry_58" /note=Original Glimmer call @bp 37065 has strength 3.0; Genemark calls start at 36843 /note=SSC: 36843-37187 CP: yes SCS: both-gm ST: NA BLAST-Start: [DUF4326 domain-containing protein [Prescottella equi] ],,NCBI, q2:s4 86.8421% 3.37942E-46 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.139, -4.660750147004694, no F: hypothetical protein SIF-BLAST: ,,[DUF4326 domain-containing protein [Prescottella equi] ],,NKT31597,73.6364,3.37942E-46 SIF-HHPRED: DUF4326 ; Domain of unknown function (DUF4326),,,PF14216.11,76.3158,99.9 SIF-Syn: Highly conserved, others also call a NKF gene around this region. CDS 37191 - 38348 /gene="59" /product="gp59" /function="HNH endonuclease" /locus tag="IsHungry_59" /note=Original Glimmer call @bp 37191 has strength 6.59; Genemark calls start at 37191 /note=SSC: 37191-38348 CP: yes SCS: both ST: SS BLAST-Start: [HNH endonuclease [Arthrobacter phage Cole] ],,NCBI, q1:s1 100.0% 0.0 GAP: 3 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.677, -4.2828813219219075, yes F: HNH endonuclease SIF-BLAST: ,,[HNH endonuclease [Arthrobacter phage Cole] ],,YP_010761024,93.2292,0.0 SIF-HHPRED: CRISPR-associated endonuclease Cas9; Inhibitor, Complex, VIRAL PROTEIN; HET: NI; 2.097A {Staphylococcus aureus},,,7ENH_A,20.0,98.3 SIF-Syn: Other genes in Zaheer and Cole were called here, very well conserved. CDS 38345 - 38674 /gene="60" /product="gp60" /function="VRR-Nuc domain protein" /locus tag="IsHungry_60" /note=Original Glimmer call @bp 38345 has strength 12.68; Genemark calls start at 38345 /note=SSC: 38345-38674 CP: yes SCS: both ST: SS BLAST-Start: [nuclease [Arthrobacter phage Zaheer] ],,NCBI, q1:s1 100.0% 1.05471E-61 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.953, -3.5088110057482766, yes F: VRR-Nuc domain protein SIF-BLAST: ,,[nuclease [Arthrobacter phage Zaheer] ],,YP_010761093,92.6606,1.05471E-61 SIF-HHPRED: Nuclease; Nuclease, HYDROLASE; HET: SO4; 1.85A {Salmonella phage SETP3} SCOP: c.52.1.35,,,4QBN_A,87.156,99.8 SIF-Syn: When compared with Gusanita and Ryan, they both call nuclease around this area and not a VRR-Nuc domain protein. CDS 38671 - 39450 /gene="61" /product="gp61" /function="hypothetical protein" /locus tag="IsHungry_61" /note=Original Glimmer call @bp 38671 has strength 11.49; Genemark calls start at 38671 /note=SSC: 38671-39450 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein QEO78_gp40 [Arthrobacter phage Popper] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.444, -4.197870532213559, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO78_gp40 [Arthrobacter phage Popper] ],,YP_010761161,97.6834,0.0 SIF-HHPRED: VCIP135_N ; VCIP135 N-terminal,,,PF19437.4,24.7104,95.8 SIF-Syn: Other genes called in this area in phage Popper, also with NKF CDS 39447 - 39662 /gene="62" /product="gp62" /function="hypothetical protein" /locus tag="IsHungry_62" /note=Original Glimmer call @bp 39510 has strength 5.02; Genemark calls start at 39510 /note=SSC: 39447-39662 CP: yes SCS: both-cs ST: NA BLAST-Start: GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.779, -3.7960205838585104, yes F: hypothetical protein SIF-BLAST: SIF-HHPRED: SIF-Syn: Has no known function, but is highly conserved since Ryan and Gusanita also calls genes with no known function towards the end. CDS 39886 - 40158 /gene="63" /product="gp63" /function="hypothetical protein" /locus tag="IsHungry_63" /note=Original Glimmer call @bp 39886 has strength 7.88; Genemark calls start at 39775 /note=SSC: 39886-40158 CP: yes SCS: both-gl ST: NI BLAST-Start: [hypothetical protein QEO78_gp37 [Arthrobacter phage Popper] ],,NCBI, q1:s1 100.0% 9.62311E-46 GAP: 223 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.809, -3.2558475833917586, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein QEO78_gp37 [Arthrobacter phage Popper] ],,YP_010761164,92.1348,9.62311E-46 SIF-HHPRED: SIF-Syn: There is no known function but it`s highly conserved; compared to Gusanita which has other NKF calls. CDS 40246 - 40572 /gene="64" /product="gp64" /function="HNH endonuclease" /locus tag="IsHungry_64" /note=Original Glimmer call @bp 40276 has strength 6.12; Genemark calls start at 40246 /note=SSC: 40246-40572 CP: yes SCS: both-gm ST: SS BLAST-Start: [HNH endonuclease [Arthrobacter phage Popper] ],,NCBI, q1:s1 100.0% 3.78479E-71 GAP: 87 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.914, -4.924073590240254, no F: HNH endonuclease SIF-BLAST: ,,[HNH endonuclease [Arthrobacter phage Popper] ],,YP_010761165,98.1481,3.78479E-71 SIF-HHPRED: CRISPR-associated endonuclease Cas9; Inhibitor, Complex, VIRAL PROTEIN; HET: NI; 2.097A {Staphylococcus aureus},,,7ENH_A,55.5556,98.0 SIF-Syn: Other phages Popper and Eleaser call a HNH endonuclease here, highly conserved.