CDS 1 - 615 /gene="1" /product="gp1" /function="ParB-like nuclease domain" /locus tag="Jingles_1" /note=Original Glimmer call @bp 1 has strength 8.43; Genemark calls start at 1 /note=SSC: 1-615 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein FDJ43_gp01 [Microbacterium phage Koji] ],,NCBI, q1:s1 100.0% 2.67461E-143 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.643, -5.309117108752867, no F: ParB-like nuclease domain SIF-BLAST: ,,[hypothetical protein FDJ43_gp01 [Microbacterium phage Koji] ],,YP_009624200,100.0,2.67461E-143 SIF-HHPRED: SIF-Syn: hypothetical protein /note=Current Gene Number: 49 /note=Did the start site stay the same or change: Stayed the same /note=Do the Glimmer and Genemark start sites agree? Y /note=What about Starterator? Y /note=Is the ORF chosen the longest ORF: N /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on blast and with which phage? Y-Albedo /note=Z score = 2.759 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. Nothing disagreed /note= /note=Synteny: /note=Phagesdb blast- For any potential functional match, the E value should be 10^-7 or less: Y /note=Blast NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: Y CDS 608 - 2026 /gene="2" /product="gp2" /function="terminase" /locus tag="Jingles_2" /note=Original Glimmer call @bp 608 has strength 11.39; Genemark calls start at 620 /note=SSC: 608-2026 CP: yes SCS: both-gl ST: SS BLAST-Start: [terminase [Microbacterium phage BouleyBill]],,NCBI, q1:s1 100.0% 0.0 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.597, -3.221100372932034, no F: terminase SIF-BLAST: ,,[terminase [Microbacterium phage BouleyBill]],,XGU07175,99.5763,0.0 SIF-HHPRED: Terminase, large subunit; phage defense, pattern-recognition receptor, nlr, stand, atpase, ANTIVIRAL PROTEIN; HET: ATP;{Salmonella enterica},,,8DGC_E,95.5508,100.0 SIF-Syn: Terminase found upstream from the portal protein as in BouleyBill /note=Current Gene Number: 2 /note=Did the start site stay the same or change: Same /note=Do the Glimmer and Genemark start sites agree? No - called based on Glimmer and Starterator /note=Does Starterator agree? no /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes, BouleyBill /note=Z score = 2.597 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. The start site was kept because it has a 1:1 alignment and longer ORF. /note= /note=Synteny: y /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: BouleyBill /note=BLAST NCBI function match with E value should be 10^-7 or less: BouleyBill /note=HHPRED Match with > 90% probability: Terminase /note=Conserved Domain: n/a CDS 2028 - 3368 /gene="3" /product="gp3" /function="portal protein" /locus tag="Jingles_3" /note=Original Glimmer call @bp 2028 has strength 16.52; Genemark calls start at 2028 /note=SSC: 2028-3368 CP: no SCS: both ST: SS BLAST-Start: [portal protein [Microbacterium phage Bustleton]],,NCBI, q1:s1 100.0% 0.0 GAP: 1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.028, -4.45540384879315, no F: portal protein SIF-BLAST: ,,[portal protein [Microbacterium phage Bustleton]],,QCG77909,98.2063,0.0 SIF-HHPRED: Portal protein; Bacteriophage, Mycobacteriophage Bxb1, capsid, portal, VIRUS, VIRAL PROTEIN;{Mycobacterium phage Bxb1},,,9D9W_Fi,86.7713,99.9 SIF-Syn: portal protein found upstream from the capsid maturation protease as in BouleyBill /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: No but this start prevents excess overlap /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes, Bouleybill /note=Z score = 2.028 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. The start site was kept because it has a 1:1 alignment, agrees with Starterator, and is visibly apparent on Genemark. /note= /note=Synteny: Y /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: BouleyBill, E value of 0 /note=BLAST NCBI function match with E value should be 10^-7 or less: portal protein, 2e-24 /note=HHPRED Match with > 90% probability: portal protein /note=Conserved Domain: n/a CDS 3370 - 4044 /gene="4" /product="gp4" /function="capsid maturation protease" /locus tag="Jingles_4" /note=Original Glimmer call @bp 3370 has strength 14.68; Genemark calls start at 3370 /note=SSC: 3370-4044 CP: no SCS: both ST: SS BLAST-Start: [capsid maturation protease [Microbacterium phage BouleyBill]],,NCBI, q1:s1 100.0% 5.65271E-163 GAP: 1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.14, -4.211732089774149, no F: capsid maturation protease SIF-BLAST: ,,[capsid maturation protease [Microbacterium phage BouleyBill]],,XGU07177,100.0,5.65271E-163 SIF-HHPRED: Portal protein; Archaeal virus, portal, portal capsid interface, Mg ions, VIRUS; HET: HIP, MG; 2.342A {Haloferax tailed virus 1},,,8QQN_PD,48.2143,99.6 SIF-Syn: capsid maturation protease found downstream from the portal protein as in BouleyBill /note=Current Gene Number: 4 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? yes /note=Does Starterator agree? yes /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? yes, BouleyBill and Sinatra. /note=Z score = 2.14 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. The start site was kept because it is 1:1 aligned and agrees with Starterator. /note= /note=Synteny: yes /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: /note=BLAST NCBI function match with E value should be 10^-7 or less: BouleyBill /note=HHPRED Match with > 90% probability: portal capsid interface/portal protein /note=Conserved Domain: n/a CDS 4125 - 4673 /gene="5" /product="gp5" /function="scaffolding protein" /locus tag="Jingles_5" /note=Original Glimmer call @bp 4125 has strength 13.23; Genemark calls start at 4125 /note=SSC: 4125-4673 CP: yes SCS: both ST: SS BLAST-Start: [scaffolding protein [Microbacterium phage PrincePhergus] ],,NCBI, q1:s1 100.0% 8.2045E-127 GAP: 80 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.343, -3.7091199845487814, no F: scaffolding protein SIF-BLAST: ,,[scaffolding protein [Microbacterium phage PrincePhergus] ],,QBZ72874,100.0,8.2045E-127 SIF-HHPRED: Coronin-1A; coiled coil, coronin 1, PROTEIN BINDING; 1.2A {N/A},,,2AKF_A,18.1319,95.8 SIF-Syn: scaffolding protein found upstream from the major capsid protein as in BouleyBill /note=Current Gene Number: /note=Did the start site stay the same or change: Y /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: No /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes, PrincePhergus, Pherbot. /note=Z score = 2.343 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. /note=The start site was kept because it has a 1:1 alignment, and agrees with Starterator. /note= /note=Synteny: yes /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Bustleton /note=BLAST NCBI function match with E value should be 10^-7 or less: PrincePhergus, Pherbot, Bustleton /note=HHPRED Match with > 90% probability: Coronin-1A /note=Conserved Domain: NA CDS 4718 - 5671 /gene="6" /product="gp6" /function="major capsid protein" /locus tag="Jingles_6" /note=Original Glimmer call @bp 4718 has strength 15.82; Genemark calls start at 4718 /note=SSC: 4718-5671 CP: yes SCS: both ST: SS BLAST-Start: [major capsid protein [Microbacterium phage PrincePhergus]],,NCBI, q1:s1 98.4227% 0.0 GAP: 44 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.152, -2.0949393225970705, yes F: major capsid protein SIF-BLAST: ,,[major capsid protein [Microbacterium phage PrincePhergus]],,QBZ72875,98.1013,0.0 SIF-HHPRED: Major capsid protein; HK97-fold, T=7, tailed bacteriophage, VIRUS; 2.2A {Microbacterium phage Oxtober96},,,8ECO_B,97.7918,100.0 SIF-Syn: Synteny: PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: PrincePhergus BLAST NCBI function match with E value should be 10^-7 or less: PrincePhergus HHPRED Match with > 90% probability: Major capsid protein Conserved Domain: n/a /note=Current Gene Number: 6 /note=Did the start site stay the same or change: Same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Starterator is not loading for this gene. /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y, PrincePhergus /note=Z score = 3.152 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. All evidence agreed. /note= /note=[Major Capsid Protien] found [downstream] from the [Scaffolding Protein ] as in [Sinatra] CDS 5674 - 5934 /gene="7" /product="gp7" /function="hypothetical protein" /locus tag="Jingles_7" /note=Original Glimmer call @bp 5674 has strength 14.26; Genemark calls start at 5674 /note=SSC: 5674-5934 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHERBOT_7 [Microbacterium phage Pherbot] ],,NCBI, q1:s2 100.0% 9.33801E-29 GAP: 2 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.557, -4.151529987991346, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHERBOT_7 [Microbacterium phage Pherbot] ],,QBZ73058,93.9759,9.33801E-29 SIF-HHPRED: SIF-Syn: hypothetical protein /note=Current Gene Number: 7 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: No, but the gap is very small /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? No /note=Z score = 2.557 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. The start site was kept because it has a 1:1 alignment, agrees with Starterator, and Glimmer and GeneMark agree. /note=Synteny: yes, though not as strong matches /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Bustleton /note=BLAST NCBI function match with E value should be 10^-7 or less: Pherbot, Bustleton /note=HHPRED Match with > 90% probability: n/a /note=Conserved Domain: n/a CDS 5991 - 6350 /gene="8" /product="gp8" /function="hypothetical protein" /locus tag="Jingles_8" /note=Original Glimmer call @bp 5991 has strength 11.71; Genemark calls start at 5991 /note=SSC: 5991-6350 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_KAUALA_8 [Microbacterium phage Kauala]],,NCBI, q1:s1 100.0% 1.07341E-52 GAP: 56 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.289, -4.352843561762388, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_KAUALA_8 [Microbacterium phage Kauala]],,QNL31020,81.3559,1.07341E-52 SIF-HHPRED: SIF-Syn: hypothetical protein /note=Current Gene Number: 8 /note=Did the start site stay the same or change: Same /note=Do the Glimmer and Genemark start sites agree? Yes /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: No /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Y; Kauala /note=Z score = 2.289 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. The start site was kept because it has a 1:1 alignment, longer ORF, and agrees with Starterator. /note=Synteny: Y /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Bustleton /note=BLAST NCBI function match with E value should be 10^-7 or less: Pherbot /note=HHPRED Match with > 90% probability: n/a /note=Conserved Domain: n/a CDS 6369 - 6791 /gene="9" /product="gp9" /function="hypothetical protein" /locus tag="Jingles_9" /note=Original Glimmer call @bp 6369 has strength 9.79; Genemark calls start at 6369 /note=SSC: 6369-6791 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_SINATRA_9 [Microbacterium phage Sinatra]],,NCBI, q1:s1 100.0% 3.51739E-96 GAP: 18 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.927, -2.442961286954254, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_SINATRA_9 [Microbacterium phage Sinatra]],,QDH92737,99.2857,3.51739E-96 SIF-HHPRED: gp50 - Portal adaptor protein; Bacteriophage, virus, needle, baseplate; 3.4A {Clostridioides difficile},,,9GB7_W,69.2857,99.4 SIF-Syn: hypothetical protein /note=Did the start site stay the same or change: Y /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Yes that makes sense without too much overlap /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes, Sinatra /note=Z score = 2.927 /note= /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. The start site was kept because it has a 1:1 alignment, longer ORF, and agrees with Starterator. /note=Synteny: yes /note=the presence of two or more homologous genes on the same chromosome, or the conservation of the same blocks of genes in the same relative order between the genomes of different species /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: PrincePhergus & Sinatra /note=BLAST NCBI function match with E value should be 10^-7 or less: PrincePhergus & Sinatra /note=Conserved Domain: n/a CDS 6763 - 7173 /gene="10" /product="gp10" /function="hypothetical protein" /locus tag="Jingles_10" /note=Original Glimmer call @bp 6763 has strength 18.33; Genemark calls start at 6763 /note=SSC: 6763-7173 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_SINATRA_10 [Microbacterium phage Sinatra]],,NCBI, q1:s1 100.0% 1.76047E-90 GAP: -29 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.529, -3.4470622626190464, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_SINATRA_10 [Microbacterium phage Sinatra]],,QDH92738,100.0,1.76047E-90 SIF-HHPRED: DUF6093 ; Family of unknown function (DUF6093),,,PF19586.5,77.2059,97.0 SIF-Syn: hypothetical protein /note=Current Gene Number: 10 /note=Did the start site stay the same or change: Stayed the same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: N /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Sinatra /note=Z score = 2.529 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site: Everything agrees /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: CDS 7170 - 7511 /gene="11" /product="gp11" /function="hypothetical protein" /locus tag="Jingles_11" /note=Original Glimmer call @bp 7170 has strength 17.0; Genemark calls start at 7170 /note=SSC: 7170-7511 CP: no SCS: both ST: NI BLAST-Start: [hypothetical protein FDJ43_gp11 [Microbacterium phage Koji] ],,NCBI, q1:s1 100.0% 2.83531E-75 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.927, -2.583959800616441, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDJ43_gp11 [Microbacterium phage Koji] ],,YP_009624210,100.0,2.83531E-75 SIF-HHPRED: Minor_capsid_2 ; Minor capsid protein,,,PF11114.13,78.7611,99.6 SIF-Syn: Synteny: Y PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y BLAST NCBI function match with E value should be 10^-7 or less: Y HHPRED Match with > 90% probability: Y Conserved Domain: /note=Current Gene Number: 11 /note=Did the start site stay the same or change: Stayed the same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y, Sinatra /note=Z score = 2.927 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site: Everything agrees CDS 7514 - 7873 /gene="12" /product="gp12" /function="tail terminator" /locus tag="Jingles_12" /note=Original Glimmer call @bp 7514 has strength 7.58; Genemark calls start at 7514 /note=SSC: 7514-7873 CP: no SCS: both ST: NI BLAST-Start: [hypothetical protein SEA_PHERBOT_12 [Microbacterium phage Pherbot] ],,NCBI, q1:s1 100.0% 6.35503E-79 GAP: 2 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.048, -4.490107201936413, no F: tail terminator SIF-BLAST: ,,[hypothetical protein SEA_PHERBOT_12 [Microbacterium phage Pherbot] ],,QBZ73063,100.0,6.35503E-79 SIF-HHPRED: terminator protein gp8; Xanthomonas phage, head-to-tail connector, tail, VIRUS; 3.6A {Xanthomonas phage phiXacJX1},,,9LBN_K,94.1176,98.2 SIF-Syn: Tail Terminator found downstream of Major Capsid Protein as in Sinatra (6 Start) /note=Current Gene Number: 12 /note=Did the start site stay the same or change: Stayed the same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y /note=Z score = 2.048 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. Everything agrees. /note=Synteny: Y /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: CDS 7873 - 8100 /gene="13" /product="gp13" /function="hypothetical protein" /locus tag="Jingles_13" /note=Original Glimmer call @bp 7873 has strength 17.06; Genemark calls start at 7873 /note=SSC: 7873-8100 CP: no SCS: both ST: NI BLAST-Start: [hypothetical protein FDJ43_gp13 [Microbacterium phage Koji] ],,NCBI, q1:s1 100.0% 4.71318E-46 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.927, -2.5052746077145835, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDJ43_gp13 [Microbacterium phage Koji] ],,YP_009624212,100.0,4.71318E-46 SIF-HHPRED: SIF-Syn: hypothetical protein /note=Current Gene Number: 13 /note=Did the start site stay the same or change: SAME /note=Do the Glimmer and Genemark start sites agree? YES /note=Does Starterator agree? YES /note=Is the ORF chosen the longest ORF:YES /note=Is all of the coding potential captured on GeneMark file: /note=Is there a 1:1 alignment on BLAST and with which phage? KOJI /note=Z score = 2.927 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. /note=Synteny: Y /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: NA /note=Conserved Domain: CDS 8113 - 8610 /gene="14" /product="gp14" /function="tail assembly chaperone" /locus tag="Jingles_14" /note=Original Glimmer call @bp 8113 has strength 15.86; Genemark calls start at 8113 /note=SSC: 8113-8610 CP: no SCS: both ST: NI BLAST-Start: [major tail protein [Microbacterium phage PrincePhergus]],,NCBI, q1:s1 100.0% 1.32858E-111 GAP: 12 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.059, -2.2364030944336752, yes F: tail assembly chaperone SIF-BLAST: ,,[major tail protein [Microbacterium phage PrincePhergus]],,QBZ72883,98.1928,1.32858E-111 SIF-HHPRED: Phage_TTP_16 ; Phage tail tube protein,,,PF25595.1,94.5455,100.0 SIF-Syn: major tail protein found downstream from tail terminator as in BouleyBill /note=Current Gene Number: 14 /note=Did the start site stay the same or change: Same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y, PrincePhergus /note=Z score = 3.059 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. Everything agreed. /note=Synteny: Y /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: CDS 8640 - 9188 /gene="15" /product="gp15" /function="tail assembly chaperone" /locus tag="Jingles_15" /note=Original Glimmer call @bp 8640 has strength 17.87; Genemark calls start at 8640 /note=SSC: 8640-9188 CP: no SCS: both ST: NI BLAST-Start: [tail assembly chaperone [Microbacterium phage PrincePhergus] ],,NCBI, q1:s1 100.0% 1.30754E-129 GAP: 29 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.568, -3.8722383622006915, yes F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Microbacterium phage PrincePhergus] ],,QBZ72884,100.0,1.30754E-129 SIF-HHPRED: SIF-Syn: /note=Current Gene Number: 15 /note=Did the start site stay the same or change: SAME /note=Do the Glimmer and Genemark start sites agree? YES /note=Does Starterator agree? /note=Is the ORF chosen the longest ORF: /note=Is all of the coding potential captured on GeneMark file: /note=Is there a 1:1 alignment on BLAST and with which phage? /note=Z score = /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. The start site was kept because it has a 1:1 alignment, longer ORF, and agrees with Starterator. /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: /note=BLAST NCBI function match with E value should be 10^-7 or less: /note=HHPRED Match with > 90% probability: /note=Conserved Domain: CDS join(8640..9146,9146..9577) /gene="16" /product="gp16" /function="tail assembly chaperone" /locus tag="Jingles_16" /note= /note=SSC: 8640-9577 CP: yes SCS: neither ST: NI BLAST-Start: [tail assembly chaperone [Microbacterium phage PrincePhergus] ],,NCBI, q1:s1 100.0% 0.0 GAP: -549 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.568, -3.8722383622006915, yes F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Microbacterium phage PrincePhergus] ],,QBZ72885,100.0,0.0 SIF-HHPRED: SIF-Syn: CDS 9596 - 12004 /gene="17" /product="gp17" /function="tape measure protein" /locus tag="Jingles_17" /note=Original Glimmer call @bp 9596 has strength 14.89; Genemark calls start at 9596 /note=SSC: 9596-12004 CP: no SCS: both ST: NI BLAST-Start: [tape measure protein [Microbacterium phage BouleyBill]],,NCBI, q1:s1 100.0% 0.0 GAP: 17 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.152, -1.953940808934884, yes F: tape measure protein SIF-BLAST: ,,[tape measure protein [Microbacterium phage BouleyBill]],,XGU07190,99.2519,0.0 SIF-HHPRED: Tape Measure Protein, gp57; phage tail, tail tip, tape measure protein, VIRAL PROTEIN; 3.7A {Staphylococcus virus 80alpha},,,6V8I_AF,15.7107,100.0 SIF-Syn: tape measure protein found upstream from minor tail protein as in BouleyBill /note=Current Gene Number: 17 /note=Did the start site stay the same or change: Same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? /note=Z score = /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. /note=Synteny: Y /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: CDS 12001 - 12771 /gene="18" /product="gp18" /function="hypothetical protein" /locus tag="Jingles_18" /note=Original Glimmer call @bp 12001 has strength 17.02; Genemark calls start at 12001 /note=SSC: 12001-12771 CP: no SCS: both ST: NI BLAST-Start: [minor tail protein [Microbacterium phage BouleyBill]],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.927, -2.5052746077145835, yes F: hypothetical protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage BouleyBill]],,XGU07191,100.0,0.0 SIF-HHPRED: HYPOTHETICAL PROTEIN 19.1; VIRAL PROTEIN, DISTAL TAIL PROTEIN; 2.95A {BACILLUS PHAGE SPP1},,,2X8K_B,99.6094,100.0 SIF-Syn: hypothetical protein /note=Current Gene Number: 18 /note=Did the start site stay the same or change: YES /note=Do the Glimmer and Genemark start sites agree? YES /note=Does Starterator agree? YES /note=Is the ORF chosen the longest ORF: TRUE /note=Is all of the coding potential captured on GeneMark file: YES /note=Is there a 1:1 alignment on BLAST and with which phage? BouleyBill /note=Z score = 2.927 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. Everything lines up - Everything Agrees /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: YES /note=HHPRED Match with > 90% probability: Hypothetical Protein 100% /note=Conserved Domain: CDS 12771 - 15194 /gene="19" /product="gp19" /function="minor tail protein" /locus tag="Jingles_19" /note=Original Glimmer call @bp 12771 has strength 16.22; Genemark calls start at 12771 /note=SSC: 12771-15194 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Microbacterium phage BouleyBill]],,NCBI, q1:s1 100.0% 0.0 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.778, -2.7653702713535186, yes F: minor tail protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage BouleyBill]],,XGU07192,100.0,0.0 SIF-HHPRED: Tail-Associated Lysin, gp59; phage tail, tail tip, tape measure protein, VIRAL PROTEIN; 3.7A {Staphylococcus virus 80alpha},,,6V8I_CE,39.0335,99.9 SIF-Syn: Hypothetical protein found downstream from the minor tail protein as in BouleyBill and /note=Current Gene Number: 12771 /note=Did the start site stay the same or change: Same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file: /note=Is there a 1:1 alignment on BLAST and with which phage? Y, BouleyBill /note=Z score = 2.778 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site: All starts agreed /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: N/A CDS 15196 - 15384 /gene="20" /product="gp20" /function="hypothetical protein" /locus tag="Jingles_20" /note=Original Glimmer call @bp 15196 has strength 15.6; Genemark calls start at 15196 /note=SSC: 15196-15384 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CHICKENKING_20 [Microbacterium phage ChickenKing]],,NCBI, q1:s1 100.0% 3.49281E-34 GAP: 1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.446, -3.54831954703195, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CHICKENKING_20 [Microbacterium phage ChickenKing]],,QFG04690,98.3871,3.49281E-34 SIF-HHPRED: CBM-cenC domain-containing protein; bacteriophage, phage, contractile, phi812, baseplate, VIRUS; 7.3A {Staphylococcus phage 812},,,9EUF_q,83.871,96.7 SIF-Syn: hypothetical protein /note=Current Gene Number: 15196 /note=Did the start site stay the same or change: same /note=Do the Glimmer and Genemark start sites agree? y /note=Does Starterator agree? y /note=Is the ORF chosen the longest ORF: y /note=Is all of the coding potential captured on GeneMark file: y /note=Is there a 1:1 alignment on BLAST and with which phage? y, Chicken King /note=Z score =2.446 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start sites? everything is the same /note= /note=Synteny /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: y /note=BLAST NCBI function match with E value should be 10^-7 or less: y /note=HHPRED Match with > 90% probability: y /note=Conserved Domain: N/A CDS 15385 - 15984 /gene="21" /product="gp21" /function="hypothetical protein" /locus tag="Jingles_21" /note=Original Glimmer call @bp 15385 has strength 11.56; Genemark calls start at 15385 /note=SSC: 15385-15984 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_BOULEYBILL_21 [Microbacterium phage BouleyBill]],,NCBI, q1:s1 100.0% 7.1174E-142 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.953, -4.555276994448509, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_BOULEYBILL_21 [Microbacterium phage BouleyBill]],,XGU07194,100.0,7.1174E-142 SIF-HHPRED: CBM-cenC domain-containing protein; bacteriophage, phage, contractile, phi812, baseplate, VIRUS; 7.3A {Staphylococcus phage 812},,,9EUF_q,26.1307,92.9 SIF-Syn: Hypothetical protein /note=Current Gene Number: 15385 /note=Did the start site stay the same or change: Same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file:Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y, BouleyBill /note=Z score = 1.953 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site: All starts agree /note= /note=Synteny /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: N/A CDS 15984 - 16469 /gene="22" /product="gp22" /function="minor tail protein" /locus tag="Jingles_22" /note=Original Glimmer call @bp 15984 has strength 12.44; Genemark calls start at 15984 /note=SSC: 15984-16469 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHERBOT_22 [Microbacterium phage Pherbot]],,NCBI, q1:s1 100.0% 4.98917E-94 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.014, -2.394485424036831, yes F: minor tail protein SIF-BLAST: ,,[hypothetical protein SEA_PHERBOT_22 [Microbacterium phage Pherbot]],,QBZ73073,96.8944,4.98917E-94 SIF-HHPRED: Gp15 protein; Listeria, homotrimeric, receptor binding protein, Bacteriophage, VIRAL PROTEIN; HET: 1PE, ACT; 1.7A {Listeria phage PSA},,,6R5W_A,72.0497,98.9 SIF-Syn: Main tail protein found upstream from the Main Tail Protein as in BouleyBill. /note=Current Gene Number: 15984 /note=Did the start site stay the same or change: Same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file:Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y /note=Z score = 1.571 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. Everything agrees /note= /note=Synteny /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: N/A CDS 16471 - 18600 /gene="23" /product="gp23" /function="minor tail protein" /locus tag="Jingles_23" /note=Original Glimmer call @bp 16471 has strength 9.97; Genemark calls start at 16471 /note=SSC: 16471-18600 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Microbacterium phage Sinatra]],,NCBI, q1:s1 100.0% 0.0 GAP: 1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.977, -4.565177092733586, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage Sinatra]],,QDH92751,98.5896,0.0 SIF-HHPRED: Chitinase B; chitinase, TIM barrel, necrotic enteritis, HYDROLASE; HET: PIN, PG4; 1.6A {Clostridium perfringens},,,8OVR_A,45.5571,100.0 SIF-Syn: Minor tail protein found downstream from the Lysin A function protein as in BouleyBill. /note=Current Gene Number: 16471 /note=Did the start site stay the same or change: same /note=Do the Glimmer and Genemark start sites agree? y /note=Does Starterator agree? y /note=Is the ORF chosen the longest ORF: y /note=Is all of the coding potential captured on GeneMark file: y /note=Is there a 1:1 alignment on BLAST and with which phage? yes, Sinatra /note=Z score =1.977 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start sites? everything is the same and there is no evidence for anything to change /note= /note=Synteny /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: N/A CDS 18630 - 19625 /gene="24" /product="gp24" /function="lysin A" /locus tag="Jingles_24" /note=Original Glimmer call @bp 18630 has strength 14.18; Genemark calls start at 18630 /note=SSC: 18630-19625 CP: yes SCS: both ST: SS BLAST-Start: [lysin A [Microbacterium phage PrincePhergus]],,NCBI, q1:s1 100.0% 0.0 GAP: 29 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.927, -2.5052746077145835, yes F: lysin A SIF-BLAST: ,,[lysin A [Microbacterium phage PrincePhergus]],,QBZ72893,99.6979,0.0 SIF-HHPRED: peptidoglycan recognition protein 3; peptidoglycan recognition protein, chitin-binding domain, amidase, HYDROLASE; 2.701A {Branchiostoma belcheri tsingtauense},,,4Z8I_A,37.7644,99.3 SIF-Syn: Lysin A found downstream from minor tail protein as seen in Sinatra /note=Current Gene Number: 18630 /note=Did the start site stay the same or change: Same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y, PrincePhergus /note=Z score = 2.927 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site: All starts agree /note= /note=Synteny /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: Y CDS 19645 - 20034 /gene="25" /product="gp25" /function="hypothetical protein" /locus tag="Jingles_25" /note=Original Glimmer call @bp 19645 has strength 14.48; Genemark calls start at 19645 /note=SSC: 19645-20034 CP: yes SCS: both ST: SS BLAST-Start: [membrane protein [Microbacterium phage PrincePhergus] ],,NCBI, q1:s1 100.0% 7.36897E-85 GAP: 19 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.833, -2.6453814847322845, yes F: hypothetical protein SIF-BLAST: ,,[membrane protein [Microbacterium phage PrincePhergus] ],,QBZ72894,97.6923,7.36897E-85 SIF-HHPRED: DUF7365 ; Coiled-coil region of unknown function (DUF7365),,,PF24073.1,71.3178,97.4 SIF-Syn: hypothetical protein /note=Current Gene Number: 19645 /note=Did the start site stay the same or change: same /note=Do the Glimmer and Genemark start sites agree? y /note=Does Starterator agree? y /note=Is the ORF chosen the longest ORF: y /note=Is all of the coding potential captured on GeneMark file: y /note=Is there a 1:1 alignment on BLAST and with which phage? yes, Sinatra /note=Z score = 2.833 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start sites? everything is the same and there is no evidence for anything to change /note= /note=Synteny: yes /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: N/A CDS 20034 - 20327 /gene="26" /product="gp26" /function="holin" /locus tag="Jingles_26" /note=Original Glimmer call @bp 20034 has strength 16.86; Genemark calls start at 20034 /note=SSC: 20034-20327 CP: yes SCS: both ST: SS BLAST-Start: [holin [Microbacterium phage BouleyBill]],,NCBI, q1:s1 100.0% 2.95731E-57 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.833, -2.6453814847322845, yes F: holin SIF-BLAST: ,,[holin [Microbacterium phage BouleyBill]],,XGU07199,100.0,2.95731E-57 SIF-HHPRED: Phage_holin_5_1 ; Bacteriophage A118-like holin, Hol118,,,PF06946.16,80.4124,99.8 SIF-Syn: Holin found downstream from Lysin A as in Bouley Bill /note=Current Gene Number: 20034 /note=Did the start site stay the same or change: Same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file:Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y, BouleyBill /note=Z score = 2.833 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site: All starts agree /note= /note=Synteny /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: N/A CDS complement (20385 - 20567) /gene="27" /product="gp27" /function="hypothetical protein" /locus tag="Jingles_27" /note=Original Glimmer call @bp 20567 has strength 19.22; Genemark calls start at 20582 /note=SSC: 20567-20385 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein SEA_PRINCEPHERGUS_27 [Microbacterium phage PrincePhergus]],,NCBI, q1:s6 100.0% 1.39285E-32 GAP: 11 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.927, -2.442961286954254, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PRINCEPHERGUS_27 [Microbacterium phage PrincePhergus]],,QBZ72896,90.7692,1.39285E-32 SIF-HHPRED: SIF-Syn: hypothetical protein /note=Current Gene Number: 20567 /note=Did the start site stay the same or change: Same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file:Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y, Bustleton /note=Z score = 2.927 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site: All starts agree: Most agreed on 20567 /note= /note=Synteny /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: N /note=Conserved Domain: N/A CDS complement (20579 - 20773) /gene="28" /product="gp28" /function="hypothetical protein" /locus tag="Jingles_28" /note=Original Glimmer call @bp 20773 has strength 12.39; Genemark calls start at 20773 /note=SSC: 20773-20579 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_BOULEYBILL_28 [Microbacterium phage BouleyBill]],,NCBI, q1:s1 100.0% 8.13683E-38 GAP: 23 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.327, -4.652389663202148, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_BOULEYBILL_28 [Microbacterium phage BouleyBill]],,XGU07201,100.0,8.13683E-38 SIF-HHPRED: TAPR1-like ; Telomere attrition and p53 response 1 protein-like,,,PF15251.11,73.4375,86.5 SIF-Syn: Hypothetical protein /note=Current Gene Number: 28 /note=Did the start site stay the same or change: Y /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file: y /note=Is there a 1:1 alignment on BLAST and with which phage? Y BouleyBill /note=Z score = 2.327 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. Everything was the same /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: N /note=Conserved Domain: N/A CDS complement (20797 - 21690) /gene="29" /product="gp29" /function="hypothetical protein" /locus tag="Jingles_29" /note=Original Glimmer call @bp 21690 has strength 17.54; Genemark calls start at 21690 /note=SSC: 21690-20797 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein SEA_PHERBOT_29 [Microbacterium phage Pherbot] ],,NCBI, q1:s1 100.0% 0.0 GAP: 39 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.537, -6.111027512227797, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHERBOT_29 [Microbacterium phage Pherbot] ],,QBZ73080,98.9899,0.0 SIF-HHPRED: DUF5351 ; Family of unknown function (DUF5351),,,PF17302.8,9.09091,96.3 SIF-Syn: Hypothetical Protein /note=Current Gene Number: 28 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes /note=Does Starterator agree? Yes /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes, with Bustleton /note=Z score = 2.353 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. The start site was kept because it has a 1:1 alignment and agrees with Starterator. /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: NA CDS complement (21730 - 22005) /gene="30" /product="gp30" /function="hypothetical protein" /locus tag="Jingles_30" /note=Original Glimmer call @bp 22005 has strength 17.16; Genemark calls start at 22005 /note=SSC: 22005-21730 CP: yes SCS: both ST: NI BLAST-Start: [membrane protein [Microbacterium phage BouleyBill]],,NCBI, q1:s1 100.0% 1.13343E-56 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.854, -2.6814417326944517, yes F: hypothetical protein SIF-BLAST: ,,[membrane protein [Microbacterium phage BouleyBill]],,XGU07203,100.0,1.13343E-56 SIF-HHPRED: SIF-Syn: Hypothetical protein /note=Current Gene Number: 30 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes /note=Does Starterator agree? Yes /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes, with BouleyBill /note=Z score = 2.854 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. The start site was kept because it has a 1:1 alignment and agrees with Starterator. /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: N /note=Conserved Domain: NA CDS complement (22002 - 23669) /gene="31" /product="gp31" /function="RecA-like DNA recombinase" /locus tag="Jingles_31" /note=Original Glimmer call @bp 23669 has strength 15.46; Genemark calls start at 23669 /note=SSC: 23669-22002 CP: yes SCS: both ST: NI BLAST-Start: [RecA-like DNA recombinase [Microbacterium phage Sinatra]],,NCBI, q1:s1 100.0% 0.0 GAP: -25 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.76, -2.944818356413908, yes F: RecA-like DNA recombinase SIF-BLAST: ,,[RecA-like DNA recombinase [Microbacterium phage Sinatra]],,QDH92759,100.0,0.0 SIF-HHPRED: Regulatory protein repA; replicative DNA helicase structural changes, REPLICATION; HET: SO4; 1.95A {Escherichia coli} SCOP: c.37.1.11,,,1NLF_A,40.3604,99.8 SIF-Syn: RecA-like DNA recombinase found upstream from the function. Hypothetical protein as in Sinatra. /note=Current Gene Number: 31 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes /note=Does Starterator agree? Yes /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes, with Sinatra /note=Z score = 2.76 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. The start site was kept because it has a 1:1 alignment and agrees with Starterator. /note= /note=Synteny: Yes /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: NA CDS complement (23645 - 23938) /gene="32" /product="gp32" /function="nuclease" /locus tag="Jingles_32" /note=Original Glimmer call @bp 23938 has strength 8.14; Genemark calls start at 23938 /note=SSC: 23938-23645 CP: no SCS: both ST: NI BLAST-Start: [endonuclease [Microbacterium phage Koji] ],,NCBI, q1:s1 100.0% 4.88419E-65 GAP: 3 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 0.962, -7.058469733985742, no F: nuclease SIF-BLAST: ,,[endonuclease [Microbacterium phage Koji] ],,YP_009624231,100.0,4.88419E-65 SIF-HHPRED: SIF-Syn: Nuclease found downstream from the function hypothetical protein as in Bouley Bill. /note=Current Gene Number: 32 /note=Did the start site stay the same or change: Same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Koji /note=Z score = 0.962 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. Nothing changed /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: N/A CDS complement (23942 - 24667) /gene="33" /product="gp33" /function="hypothetical protein" /locus tag="Jingles_33" /note=Original Glimmer call @bp 24667 has strength 17.33; Genemark calls start at 24667 /note=SSC: 24667-23942 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein SEA_BUSTLETON_33 [Microbacterium phage Bustleton]],,NCBI, q1:s1 100.0% 2.87828E-165 GAP: 29 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.059, -2.156361006712914, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_BUSTLETON_33 [Microbacterium phage Bustleton]],,QCG77939,97.551,2.87828E-165 SIF-HHPRED: DUF669 ; Protein of unknown function (DUF669),,,PF05037.18,51.8672,99.8 SIF-Syn: Hypothetical Protein /note=Current Gene Number: 33 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes /note=Does Starterator agree? NA /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes, with Bustleton /note=Z score = 3.059 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. The start site was kept because it has a 1:1 alignment. /note= /note=Synteny: Yes /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: NA CDS complement (24697 - 25362) /gene="34" /product="gp34" /function="ASCE ATPase" /locus tag="Jingles_34" /note=Original Glimmer call @bp 25362 has strength 17.04; Genemark calls start at 25362 /note=SSC: 25362-24697 CP: yes SCS: both ST: NI BLAST-Start: [AAA-ATPase [Microbacterium phage Koji] ],,NCBI, q1:s1 100.0% 8.1797E-158 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.003, -3.1837611541087343, yes F: ASCE ATPase SIF-BLAST: ,,[AAA-ATPase [Microbacterium phage Koji] ],,YP_009624233,100.0,8.1797E-158 SIF-HHPRED: ORF016; Annealase, SSAP, Single Strand Annealing, Single Strand Binding, Recombineering, Recombination, SaPI, Bacteriophage, Staphylococcal, Complex, SaPI induction; HET: AGS; 3.35A {Staphylococcus phage 52A},,,8RC5_1E,98.6425,99.9 SIF-Syn: ASCE ATPase found upstream from the function hypothetical protein as in Bouley Bill /note=Current Gene Number: 34 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes /note=Does Starterator agree? Yes /note=Is the ORF chosen the longest ORF: No /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes, with Koji /note=Z score = 3.003 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. The start site was kept because it has a 1:1 alignment. /note= /note=Synteny: Yes /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: NA CDS complement (25359 - 25499) /gene="35" /product="gp35" /function="hypothetical protein" /locus tag="Jingles_35" /note=Original Glimmer call @bp 25499 has strength 6.71 /note=SSC: 25499-25359 CP: no SCS: glimmer ST: NI BLAST-Start: [hypothetical protein SEA_PRINCEPHERGUS_35 [Microbacterium phage PrincePhergus]],,NCBI, q1:s1 100.0% 9.69354E-25 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.436, -3.649482460397077, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PRINCEPHERGUS_35 [Microbacterium phage PrincePhergus]],,QBZ72904,100.0,9.69354E-25 SIF-HHPRED: SIF-Syn: Hypothetical Protein /note=Current Gene Number: 35 /note=Did the start site stay the same or change: N/A /note=Do the Glimmer and Genemark start sites agree? N /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? PRINCEPHERGUS /note=Z score = 2.436 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: y /note=BLAST NCBI function match with E value should be 10^-7 or less: y /note=HHPRED Match with > 90% probability: y /note=Conserved Domain: NA CDS complement (25496 - 26659) /gene="36" /product="gp36" /function="exonuclease" /locus tag="Jingles_36" /note=Original Glimmer call @bp 26659 has strength 12.9; Genemark calls start at 26569 /note=SSC: 26659-25496 CP: no SCS: both-gl ST: NI BLAST-Start: [exonuclease [Microbacterium phage Pherbot] ],,NCBI, q1:s1 100.0% 0.0 GAP: -23 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.048, -5.399187552617544, no F: exonuclease SIF-BLAST: ,,[exonuclease [Microbacterium phage Pherbot] ],,QBZ73087,100.0,0.0 SIF-HHPRED: PD-(D/E)XK endonuclease-like domain-containing protein; Cas4 family protein, IMMUNE SYSTEM, deoxyribonuclease, hydrolase; HET: MES, SF4, SO4; 1.57A {Chroococcidiopsis thermalis},,,9IAB_A,82.6873,99.9 SIF-Syn: Exonuclease found downstream from the function DNA polyermase I as in Bouley Bill. /note=Current Gene Number: 36 /note=Did the start site stay the same or change: Change /note=Do the Glimmer and Genemark start sites agree? N /note=Does Starterator agree? /note=Is the ORF chosen the longest ORF: n /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Pherbot /note=Z score = 2.048 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: /note=BLAST NCBI function match with E value should be 10^-7 or less: N /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: y CDS complement (26637 - 28487) /gene="37" /product="gp37" /function="DNA polymerase I" /locus tag="Jingles_37" /note=Original Glimmer call @bp 28487 has strength 9.41; Genemark calls start at 28487 /note=SSC: 28487-26637 CP: yes SCS: both ST: SS BLAST-Start: [DNA polymerase I [Microbacterium phage Sinatra]],,NCBI, q1:s1 100.0% 0.0 GAP: 175 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.059, -4.466674028236667, no F: DNA polymerase I SIF-BLAST: ,,[DNA polymerase I [Microbacterium phage Sinatra]],,QDH92765,99.8377,0.0 SIF-HHPRED: DNA polymerase nu; Pol Nu, Polymerase, error-prone DNA synthesis, TRANSFERASE-DNA complex; HET: MES; 2.95A {Homo sapiens},,,4XVK_A,98.8636,100.0 SIF-Syn: ParB-like nuclease domain found upstream from the DNA binding protein as in CupcakePrincess /note=Current Gene Number: 37 /note=Did the start site stay the same or change: Stayed the same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y-Bustleton /note=Z score = 2.059 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. /note=All is true /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: Y tRNA complement (28533 - 28614) /gene="38" /product="tRNA-Stop(tca)" /locus tag="JINGLES_38" /note=tRNA-Stop(tca) CDS complement (28663 - 29091) /gene="39" /product="gp39" /function="hypothetical protein" /locus tag="Jingles_39" /note=Original Glimmer call @bp 29091 has strength 17.46; Genemark calls start at 29091 /note=SSC: 29091-28663 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PRINCEPHERGUS_39 [Microbacterium phage PrincePhergus] ],,NCBI, q3:s4 98.5916% 3.00947E-91 GAP: 81 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.927, -2.442961286954254, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PRINCEPHERGUS_39 [Microbacterium phage PrincePhergus] ],,QBZ72907,97.9021,3.00947E-91 SIF-HHPRED: SIF-Syn: Hypothetical Protein /note=Current Gene Number: 38 /note=Did the start site stay the same or change: Stayed the same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y - Bustleton /note=Z score = 2.927 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. /note=It was the only start site /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: N /note=Conserved Domain: N CDS complement (29173 - 30570) /gene="40" /product="gp40" /function="DNA helicase" /locus tag="Jingles_40" /note=Original Glimmer call @bp 30570 has strength 16.32; Genemark calls start at 30570 /note=SSC: 30570-29173 CP: yes SCS: both ST: NI BLAST-Start: [DNA helicase [Microbacterium phage BouleyBill]],,NCBI, q1:s1 99.7849% 0.0 GAP: 79 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.759, -5.328606478331837, no F: DNA helicase SIF-BLAST: ,,[DNA helicase [Microbacterium phage BouleyBill]],,XGU07211,98.2906,0.0 SIF-HHPRED: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; nucleosome, chromatin remodeler, ISWI, SNF2h, DNA BINDING PROTEIN-DNA complex; HET: ADP; 2.8A {Xenopus laevis},,,8V6V_X,96.129,100.0 SIF-Syn: DNA helicase found upstream from the DNA polymerase I as in BouleyBill /note=Current Gene Number: 39 /note=Did the start site stay the same or change: Stayed the same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: N /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y - BouleyBill /note=Z score = 1.759 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. /note=Starterator agreed; no reason to change it. /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: Y CDS complement (30650 - 30919) /gene="41" /product="gp41" /function="hypothetical protein" /locus tag="Jingles_41" /note=Original Glimmer call @bp 30919 has strength 11.05; Genemark calls start at 30919 /note=SSC: 30919-30650 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_BOULEYBILL_40 [Microbacterium phage BouleyBill]],,NCBI, q1:s1 100.0% 6.94023E-52 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.361, -3.8116689268127657, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_BOULEYBILL_40 [Microbacterium phage BouleyBill]],,XGU07212,100.0,6.94023E-52 SIF-HHPRED: SIF-Syn: hypothetical protein /note=Current Gene Number: 40 /note=Did the start site stay the same or change: Stayed the same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: N /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y - BouleyBill /note=Z score = 2.361 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. /note=All factors agreed /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: N /note=Conserved Domain: N CDS complement (30919 - 31143) /gene="42" /product="gp42" /function="hypothetical protein" /locus tag="Jingles_42" /note=Original Glimmer call @bp 31143 has strength 16.1; Genemark calls start at 31143 /note=SSC: 31143-30919 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein SEA_PRINCEPHERGUS_42 [Microbacterium phage PrincePhergus]],,NCBI, q1:s1 100.0% 1.32736E-43 GAP: 1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.446, -3.627004739933808, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PRINCEPHERGUS_42 [Microbacterium phage PrincePhergus]],,QBZ72910,100.0,1.32736E-43 SIF-HHPRED: SIF-Syn: hypothetical protein /note=Current Gene Number: 41 /note=Did the start site stay the same or change: Stayed the same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: N /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y-PrincePhergus /note=Z score = 2.446 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. There were no disagreements. /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: N /note=Conserved Domain: N CDS complement (31145 - 31963) /gene="43" /product="gp43" /function="MazG-like nucleotide pyrophosphohydrolase" /locus tag="Jingles_43" /note=Original Glimmer call @bp 31963 has strength 18.73; Genemark calls start at 31963 /note=SSC: 31963-31145 CP: yes SCS: both ST: NI BLAST-Start: [MazG-like nucleotide pyrophosphohydrolase [Microbacterium phage Pherbot] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.754, -2.958041784599676, no F: MazG-like nucleotide pyrophosphohydrolase SIF-BLAST: ,,[MazG-like nucleotide pyrophosphohydrolase [Microbacterium phage Pherbot] ],,QBZ73093,98.8971,0.0 SIF-HHPRED: putative NTP pyrophosphohydrolase; Structural Genomics, Joint Center for Structural Genomics, JCSG, Protein Structure Initiative, PSI-2, HYDROLASE; HET: MSE; 1.78A {Exiguobacterium sibiricum 255-15},,,3NL9_A,50.3676,100.0 SIF-Syn: MazG-like nucleotide pyrophosphohydrolase found upstream from the DNA helicase as in Bustleton /note=Current Gene Number: 42 /note=Did the start site stay the same or change: Stayed the same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y-Bustleton /note=Z score = 2.754 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. No disagreement /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: Y /note=Conserved Domain: Y CDS complement (31960 - 32772) /gene="44" /product="gp44" /function="hypothetical protein" /locus tag="Jingles_44" /note=Original Glimmer call @bp 32772 has strength 9.13; Genemark calls start at 32772 /note=SSC: 32772-31960 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHERBOT_44 [Microbacterium phage Pherbot] ],,NCBI, q1:s1 100.0% 0.0 GAP: -23 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.446, -4.138145081942901, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHERBOT_44 [Microbacterium phage Pherbot] ],,QBZ73094,100.0,0.0 SIF-HHPRED: SIF-Syn: hypothetical protein /note=Current Gene Number: 43 /note=Did the start site stay the same or change: Stayed the same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y-Bustleton /note=Z score = 2.446 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. /note=All agreed /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: N /note=Conserved Domain:N CDS complement (32750 - 33337) /gene="45" /product="gp45" /function="thymidylate kinase" /locus tag="Jingles_45" /note=Original Glimmer call @bp 33337 has strength 18.29; Genemark calls start at 33337 /note=SSC: 33337-32750 CP: yes SCS: both ST: NI BLAST-Start: [thymidylate kinase [Microbacterium phage Koji] ],,NCBI, q1:s1 100.0% 1.84565E-142 GAP: 33 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.927, -2.442961286954254, yes F: thymidylate kinase SIF-BLAST: ,,[thymidylate kinase [Microbacterium phage Koji] ],,YP_009624243,100.0,1.84565E-142 SIF-HHPRED: c.37.1.1 (A:) Thymidylate kinase {Human (Homo sapiens) [TaxId: 9606]} | CLASS: Alpha and beta proteins (a/b), FOLD: P-loop containing nucleoside triphosphate hydrolases, SUPFAM: P-loop containing nucleoside triphosphate hydrolases, FAM: Nucleotide and nucleoside kinases,,,SCOP_d1nn5a_,61.5385,99.5 SIF-Syn: Thymidylate kinase found downstream from the thymidylate synthase as in Bustleton /note=Current Gene Number: 44 /note=Did the start site stay the same or change: Stayed the same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y - BouleyBill /note=Z score =2.927 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. /note=All factors agreed /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: N /note=Conserved Domain: N CDS complement (33371 - 33673) /gene="46" /product="gp46" /function="hypothetical protein" /locus tag="Jingles_46" /note=Original Glimmer call @bp 33673 has strength 9.43; Genemark calls start at 33673 /note=SSC: 33673-33371 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHERBOT_46 [Microbacterium phage Pherbot] ],,NCBI, q1:s1 100.0% 7.86733E-67 GAP: 58 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.486, -5.632912100754952, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHERBOT_46 [Microbacterium phage Pherbot] ],,QBZ73096,100.0,7.86733E-67 SIF-HHPRED: SIF-Syn: hypothetical protein /note=Current Gene Number: 45 /note=Did the start site stay the same or change: Stayed the same /note=Do the Glimmer and Genemark start sites agree? Y /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: N /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? Y-BouleyBill /note=Z score = 1.486 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. No disagreements /note= /note=Synteny: /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Y /note=BLAST NCBI function match with E value should be 10^-7 or less: Y /note=HHPRED Match with > 90% probability: N /note=Conserved Domain: N CDS complement (33732 - 34046) /gene="47" /product="gp47" /function="hypothetical protein" /locus tag="Jingles_47" /note=Original Glimmer call @bp 34046 has strength 15.33; Genemark calls start at 34046 /note=SSC: 34046-33732 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_BOULEYBILL_46 [Microbacterium phage BouleyBill]],,NCBI, q1:s1 100.0% 1.58401E-66 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.778, -2.8454123590742793, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_BOULEYBILL_46 [Microbacterium phage BouleyBill]],,XGU07218,98.0769,1.58401E-66 SIF-HHPRED: SIF-Syn: Hypothetical Protein /note=Current Gene Number: 46 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes. /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes - BouleyBill /note=Z score = 2.778 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. They are the same. /note=Synteny: Hypothetical protein found downstream from thymidylate synthase as found in BouleyBill. /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: BouleyBill. /note=BLAST NCBI function match with E value should be 10^-7 or less: y - hypothetical protein - BouleyBill /note=HHPRED Match with > 90% probability: No. /note=Conserved Domain: No data. CDS complement (34046 - 34858) /gene="48" /product="gp48" /function="thymidylate synthase" /locus tag="Jingles_48" /note=Original Glimmer call @bp 34858 has strength 15.53; Genemark calls start at 34858 /note=SSC: 34858-34046 CP: yes SCS: both ST: SS BLAST-Start: [thymidylate synthase [Microbacterium phage Pherbot] ],,NCBI, q1:s1 100.0% 0.0 GAP: 110 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.927, -2.442961286954254, yes F: thymidylate synthase SIF-BLAST: ,,[thymidylate synthase [Microbacterium phage Pherbot] ],,QBZ73098,98.5185,0.0 SIF-HHPRED: Thymidylate synthase; SSGCID, Structural Genomics, Elizabethkingia anophelis, Seattle Structural Genomics Center for Infectious Disease, TRANSFERASE; HET: M0H, PGE; 1.7A {Elizabethkingia anophelis NUHP1} SCOP: d.117.1.1,,,6AUJ_C,95.9259,100.0 SIF-Syn: Thymidylate Synthase found upstream from the hypothetical protein as in Sinatra. /note=Current Gene Number: 47 /note=Did the start site stay the same or change: Stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes /note=Does Starterator agree? Yes /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes - Bustleton /note=Z score = 2.927 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. Everything is the same /note=Synteny: Thymidylate synthase found downstream from the thymidylate kinase as in BouleyBill. /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Yes /note=BLAST NCBI function match with E value should be 10^-7 or less: Yes /note=HHPRED Match with > 90% probability: Yes /note=Conserved Domain: NA CDS complement (34969 - 35268) /gene="49" /product="gp49" /function="hypothetical protein" /locus tag="Jingles_49" /note=Original Glimmer call @bp 35268 has strength 12.7; Genemark calls start at 35268 /note=SSC: 35268-34969 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CHEETO1_56 [Microbacterium phage Cheeto1]],,NCBI, q1:s1 95.9596% 1.17816E-41 GAP: 57 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.445, -3.5499432015425856, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CHEETO1_56 [Microbacterium phage Cheeto1]],,UVG34621,79.5918,1.17816E-41 SIF-HHPRED: Small CPxCG-related zinc finger protein; zinc-finger, METAL BINDING PROTEIN; NMR {Haloferax volcanii DS2},,,8Q5B_A,26.2626,97.4 SIF-Syn: Hypothetical Protein /note=Current Gene Number: 48 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes /note=Does Starterator agree? Yes /note=Is the ORF chosen the longest ORF: Y /note=Is all of the coding potential captured on GeneMark file: Y /note=Is there a 1:1 alignment on BLAST and with which phage? No /note=Z score = 2.445 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. They are the same. /note= /note=Synteny: none /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Multiple matches. (Cheeto1 ChickenKing and GaeCeo) /note=BLAST NCBI function match with E value should be 10^-7 or less: Yes /note=HHPRED Match with > 90% probability: Yes /note=Conserved Domain: No data. CDS complement (35326 - 35502) /gene="50" /product="gp50" /function="hypothetical protein" /locus tag="Jingles_50" /note=Original Glimmer call @bp 35502 has strength 8.12; Genemark calls start at 35502 /note=SSC: 35502-35326 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_BOULEYBILL_49 [Microbacterium phage BouleyBill]],,NCBI, q1:s1 100.0% 5.95458E-35 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.997, -4.811774952186101, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_BOULEYBILL_49 [Microbacterium phage BouleyBill]],,XGU07221,100.0,5.95458E-35 SIF-HHPRED: SIF-Syn: Hypothetical Protein /note=Current Gene Number: 49 /note=Did the start site stay the same or change: Stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes /note=Does Starterator agree? Yes /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes - BouleyBill /note=Z score = 1.997 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. Everything is the same /note=Synteny: Hypothetical protein found upstream from the thymidylate synthase as in BouleyBill. /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Yes /note=BLAST NCBI function match with E value should be 10^-7 or less: Yes /note=HHPRED Match with > 90% probability: No /note=Conserved Domain: NA CDS complement (35499 - 36236) /gene="51" /product="gp51" /function="hypothetical protein" /locus tag="Jingles_51" /note=Original Glimmer call @bp 36236 has strength 18.91; Genemark calls start at 36236 /note=SSC: 36236-35499 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHERBOT_52 [Microbacterium phage Pherbot] ],,NCBI, q1:s1 100.0% 9.20155E-173 GAP: 24 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.445, -3.5499432015425856, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHERBOT_52 [Microbacterium phage Pherbot] ],,QBZ73102,99.1837,9.20155E-173 SIF-HHPRED: DUF7168 ; Domain of unknown function (DUF7168),,,PF23771.1,42.0408,99.5 SIF-Syn: Hypothetical Protein /note=Current Gene Number: 50 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes. /note=Does Starterator agree? Y /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes. Pherbot. /note=Z score = 2.445 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. They are the same. /note= /note=Synteny: Hypothetical protein found upstream from thymidylate synthase as in BouleyBill /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Yes. See Below. /note=BLAST NCBI function match with E value should be 10^-7 or less: Yes. /note=HHPRED Match with > 90% probability: Yes. /note=Conserved Domain: No data. CDS complement (36261 - 36419) /gene="52" /product="gp52" /function="hypothetical protein" /locus tag="Jingles_52" /note=Original Glimmer call @bp 36419 has strength 13.4; Genemark calls start at 36419 /note=SSC: 36419-36261 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PRINCEPHERGUS_53 [Microbacterium phage PrincePhergus] ],,NCBI, q1:s1 100.0% 2.397E-30 GAP: 26 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.12, -4.273442696493174, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PRINCEPHERGUS_53 [Microbacterium phage PrincePhergus] ],,QBZ72921,100.0,2.397E-30 SIF-HHPRED: SIF-Syn: Hypothetical Protein /note=Current Gene Number: 51 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes. /note=Does Starterator agree? Yes /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes -Pherbot. /note=Z score = 2.12 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. They are the same. /note= /note=Synteny: Hypothetical protein found upstream from thymidylate synthase as in Kauala. /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Yes. /note=BLAST NCBI function match with E value should be 10^-7 or less: Yes. /note=HHPRED Match with > 90% probability: No. /note=Conserved Domain: No data. CDS complement (36446 - 37321) /gene="53" /product="gp53" /function="hypothetical protein" /locus tag="Jingles_53" /note=Original Glimmer call @bp 37321 has strength 18.17; Genemark calls start at 37321 /note=SSC: 37321-36446 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PRINCEPHERGUS_54 [Microbacterium phage PrincePhergus] ],,NCBI, q1:s1 100.0% 0.0 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.003, -2.9284886490054283, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PRINCEPHERGUS_54 [Microbacterium phage PrincePhergus] ],,QBZ72922,98.9691,0.0 SIF-HHPRED: HMUDK_hel ; 5-hmdU DNA kinase, helical domain,,,PF18723.6,90.7216,100.0 SIF-Syn: Hypothetical Protein /note=Current Gene Number: 52 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes. /note=Does Starterator agree? Yes /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes - PrincePhergus /note=Z score = 3.003 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. They are the same. /note= /note=Synteny: Hypothetical protein found upstream from thymidylate synthase as in BouleyBill. /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Yes - PrincePhergus and Sinatra /note=BLAST NCBI function match with E value should be 10^-7 or less: Yes /note=HHPRED Match with > 90% probability: Yes /note=Conserved Domain: No data. CDS complement (37314 - 37919) /gene="54" /product="gp54" /function="hypothetical protein" /locus tag="Jingles_54" /note=Original Glimmer call @bp 37919 has strength 16.59; Genemark calls start at 37919 /note=SSC: 37919-37314 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHERBOT_55 [Microbacterium phage Pherbot] ],,NCBI, q1:s1 100.0% 3.64378E-142 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.176, -4.070957012376616, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHERBOT_55 [Microbacterium phage Pherbot] ],,QBZ73105,99.5025,3.64378E-142 SIF-HHPRED: Ploopntkinase3 ; P-loop Nucleotide Kinase3,,,PF18751.7,98.0099,99.8 SIF-Syn: Hypothetical Protein /note=Current Gene Number: 52 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes. /note=Does Starterator agree? Yes /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes, Pherbot /note=Z score = 2.176 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. They are the same. /note= /note=Synteny: Hypothetical protein found upstream of thymidylate synthase as in BouleyBill. /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Yes /note=BLAST NCBI function match with E value should be 10^-7 or less: Yes. /note=HHPRED Match with > 90% probability: Yes /note=Conserved Domain: No data. CDS complement (37916 - 38260) /gene="55" /product="gp55" /function="hypothetical protein" /locus tag="Jingles_55" /note=Original Glimmer call @bp 38260 has strength 13.22; Genemark calls start at 38260 /note=SSC: 38260-37916 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_SINATRA_56 [Microbacterium phage Sinatra]],,NCBI, q1:s1 100.0% 1.36998E-75 GAP: 303 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.927, -2.523003374675015, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_SINATRA_56 [Microbacterium phage Sinatra]],,QDH92783,99.1228,1.36998E-75 SIF-HHPRED: SIF-Syn: Hypothetical Protein /note=Current Gene Number: 54 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes. /note=Does Starterator agree? Yes /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes - Sinatra. /note=Z score = 2.927 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. They are the same. /note= /note=Synteny: Hypothetical protein found upstream of thymidylate synthase as in BouleyBill. /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Yes. /note=BLAST NCBI function match with E value should be 10^-7 or less: Yes. /note=HHPRED Match with > 90% probability: No. /note=Conserved Domain: No data. CDS 38564 - 38803 /gene="56" /product="gp56" /function="hypothetical protein" /locus tag="Jingles_56" /note=Original Glimmer call @bp 38564 has strength 6.36; Genemark calls start at 38564 /note=SSC: 38564-38803 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein FDJ43_gp55 [Microbacterium phage Koji] ],,NCBI, q1:s1 100.0% 1.45874E-48 GAP: 303 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.456, -3.5448090855435126, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDJ43_gp55 [Microbacterium phage Koji] ],,YP_009624254,98.7342,1.45874E-48 SIF-HHPRED: SIF-Syn: Hypothetical Protein /note=Current Gene Number: 55 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes. /note=Does Starterator agree? Yes /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes -Koji. /note=Z score = 2.456 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. They are the same. /note= /note=Synteny: Hypothetical protein found upstream of thymidylate synthase as in BouleyBill /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Yes. /note=BLAST NCBI function match with E value should be 10^-7 or less: Yes. /note=HHPRED Match with > 90% probability: No. /note=Conserved Domain: No data. CDS 38796 - 39218 /gene="57" /product="gp57" /function="hypothetical protein" /locus tag="Jingles_57" /note=Original Glimmer call @bp 38796 has strength 9.51; Genemark calls start at 38796 /note=SSC: 38796-39218 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein FDJ43_gp56 [Microbacterium phage Koji] ],,NCBI, q1:s1 100.0% 9.74467E-92 GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.927, -2.442961286954254, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDJ43_gp56 [Microbacterium phage Koji] ],,YP_009624255,99.2857,9.74467E-92 SIF-HHPRED: SIF-Syn: Hypothetical Protein /note=Current Gene Number: 56 /note=Did the start site stay the same or change: It stayed the same /note=Do the Glimmer and Genemark start sites agree? Yes. /note=Does Starterator agree? Yes /note=Is the ORF chosen the longest ORF: Yes /note=Is all of the coding potential captured on GeneMark file: Yes /note=Is there a 1:1 alignment on BLAST and with which phage? Yes -BouleyBill. /note=Z score = 2.927 /note=Detailed reasoning / evidence of why you either changed or kept the auto-annotated start site. They are the same. /note= /note=Synteny: Hypothetical protein found upstream of thymidylate synthase as in BouleyBill. /note=PhagesDB BLAST- For any potential functional match, the E value should be 10^-7 or less: Yes. /note=BLAST NCBI function match with E value should be 10^-7 or less: Yes. /note=HHPRED Match with > 90% probability: No. /note=Conserved Domain: No data.