CDS 538 - 765 /gene="1" /product="gp1" /function="HNH endonuclease" /locus tag="Kremtemulon_1" /note= /note=SSC: 538-765 CP: yes SCS: neither ST: NA BLAST-Start: [HNH endonuclease [Mycobacterium phage BellusTerra] ],,NCBI, q1:s1 100.0% 6.92465E-46 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.572, -6.114976794123185, yes F: HNH endonuclease SIF-BLAST: ,,[HNH endonuclease [Mycobacterium phage BellusTerra] ],,YP_009005559,100.0,6.92465E-46 SIF-HHPRED: HNH endonuclease; Thermophilic bacteriophage, HNH Endonuclease, DNA nicking, HYDROLASE; 1.52A {Geobacillus virus E2},,,5H0M_A,74.6667,94.2 SIF-Syn: HNH endonuclease of same size at same locus encoded in most other A4 genomes /note=Captures additional coding potential seen by GeneMarkS CDS 803 - 1234 /gene="2" /product="gp2" /function="terminase, small subunit" /locus tag="Kremtemulon_2" /note=Original Glimmer call @bp 803 has strength 8.37; Genemark calls start at 803 /note=SSC: 803-1234 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_IRACEMA64_2 [Mycobacterium phage Iracema64] ],,NCBI, q1:s1 100.0% 7.01023E-100 GAP: 37 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.996, -2.66371296573402, yes F: terminase, small subunit SIF-BLAST: ,,[hypothetical protein SEA_IRACEMA64_2 [Mycobacterium phage Iracema64] ],,YP_009190861,100.0,7.01023E-100 SIF-HHPRED: Terminase small subunit; genome packaging, bacteriophage, DNA binding, VIRAL PROTEIN; 1.4A {Enterobacteria phage HK97},,,6Z6E_B,56.6434,97.8 SIF-Syn: Same function called for same pham in many other A4 genomes (e.g., Stink, Cintron, Abdiel) CDS 1326 - 1640 /gene="3" /product="gp3" /function="minor tail protein" /locus tag="Kremtemulon_3" /note=Original Glimmer call @bp 1326 has strength 15.32; Genemark calls start at 1326 /note=SSC: 1326-1640 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_OBAMA12_3 [Mycobacterium phage Obama12] ],,NCBI, q1:s1 100.0% 3.33716E-67 GAP: 91 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.209, -2.6835611257758942, yes F: minor tail protein SIF-BLAST: ,,[hypothetical protein PBI_OBAMA12_3 [Mycobacterium phage Obama12] ],,YP_009007195,100.0,3.33716E-67 SIF-HHPRED: L-shaped tail fiber protein p132; Bacteriophage, Siphophage, T5, baseplate, VIRAL PROTEIN; 3.45A {Escherichia virus T5},,,7QG9_L,92.3077,98.3 SIF-Syn: Found in syntenic region of other structural genes and called minor tail protein in many other A4 phages (e.g., Cintron and Spino) CDS 1645 - 2136 /gene="4" /product="gp4" /function="hypothetical protein" /locus tag="Kremtemulon_4" /note= /note=SSC: 1645-2136 CP: yes SCS: neither ST: NA BLAST-Start: [minor tail protein [Mycobacterium phage OKaNui]],,NCBI, q1:s1 100.0% 3.02991E-103 GAP: 4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.829, -3.0149320667577513, yes F: hypothetical protein SIF-BLAST: ,,[minor tail protein [Mycobacterium phage OKaNui]],,QKW95478,58.0645,3.02991E-103 SIF-HHPRED: Collagen alpha-1(I) chain; NATIVE, IN SITU, Molecular envelope, TRIPLE-helical, SUPERMOLECULAR, supramolecular, PACKING STRUCTURE, STRUCTURAL PROTEIN-CONTRACTILE PROTEIN COMPLEX, Collagen, Extracellular; HET: HYP, LYZ; 5.16A {Rattus norvegicus} SCOP: i.25.1.1,,,3HQV_C,71.7791,83.8 SIF-Syn: Truncation of locus encoding minor tail protein in other A4 phages /note=This gene has very high sequence similarity to the first half of the same locus in other A4 phages. Sequence alignment shows that about 490 bp into the orf, there is a 2 bp change resulting in a premature stop codon. /note= /note=Not called as minor tail protein because truncated protein product HHPRED hits to collagen-rich proteins fall below the 90% probability threshold CDS 2722 - 3387 /gene="5" /product="gp5" /function="hypothetical protein" /locus tag="Kremtemulon_5" /note=Original Glimmer call @bp 2722 has strength 6.0; Genemark calls start at 2722 /note=SSC: 2722-3387 CP: yes SCS: both ST: NA BLAST-Start: [minor tail protein [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 1.15508E-146 GAP: 585 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.329, -4.00119595367518, yes F: hypothetical protein SIF-BLAST: ,,[minor tail protein [Mycobacterium phage Cintron] ],,YP_010062645,100.0,1.15508E-146 SIF-HHPRED: SIF-Syn: Only small number of A4 phages have this annotated as a minor tail protein; however, this was not supported by HHPRED data. /note=Large gap between genes 4 and 5 due to premature stop codon in gene 4. A reverse gene was called by both Glimmer and GeneMark between genes 4 and 5 (start: 2,604 stop: 1,945) but this broke rules of gene overlap and forward/reverse switching, and resulting product had no homology to other phage proteins (orpham). CDS 3366 - 3758 /gene="6" /product="gp6" /function="hypothetical protein" /locus tag="Kremtemulon_6" /note=Original Glimmer call @bp 3366 has strength 10.76; Genemark calls start at 3384 /note=SSC: 3366-3758 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein KIY71_gp06 [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 1.89844E-87 GAP: -22 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.67, -3.812150084159046, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein KIY71_gp06 [Mycobacterium phage Cintron] ],,YP_010062646,99.2308,1.89844E-87 SIF-HHPRED: SIF-Syn: /note=Had hard time choosing between start at 3366 or 3384 as Glimmer and GeneMark each called one or the other. 3384 gives the ideal -4 bp overlap; however, 3366 was by far the most annotated start on Starterator. CDS 3781 - 4029 /gene="7" /product="gp7" /function="hypothetical protein" /locus tag="Kremtemulon_7" /note=Original Glimmer call @bp 3781 has strength 17.54; Genemark calls start at 3781 /note=SSC: 3781-4029 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein FGG45_gp07 [Mycobacterium phage Arturo] ],,NCBI, q1:s1 100.0% 4.98989E-47 GAP: 22 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.209, -2.6835611257758942, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FGG45_gp07 [Mycobacterium phage Arturo] ],,YP_009638664,100.0,4.98989E-47 SIF-HHPRED: SIF-Syn: CDS 4029 - 5504 /gene="8" /product="gp8" /function="lysin A" /locus tag="Kremtemulon_8" /note=Original Glimmer call @bp 4029 has strength 7.97; Genemark calls start at 4029 /note=SSC: 4029-5504 CP: yes SCS: both ST: SS BLAST-Start: [endolysin [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 0.0 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.278, -4.107221089219364, no F: lysin A SIF-BLAST: ,,[endolysin [Mycobacterium phage Cintron] ],,YP_010062648,100.0,0.0 SIF-HHPRED: Peptidase_M15_4 ; D-alanyl-D-alanine carboxypeptidase,,,PF13539.9,13.6456,97.9 SIF-Syn: lysin A called at this position for most A4 phages CDS 5501 - 5926 /gene="9" /product="gp9" /function="holin" /locus tag="Kremtemulon_9" /note=Original Glimmer call @bp 5501 has strength 13.75; Genemark calls start at 5501 /note=SSC: 5501-5926 CP: yes SCS: both ST: SS BLAST-Start: [holin [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 2.41161E-93 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.66, -3.3861058366776207, yes F: holin SIF-BLAST: ,,[holin [Mycobacterium phage Cintron] ],,YP_010062649,100.0,2.41161E-93 SIF-HHPRED: Phage_holin_7_1 ; Mycobacterial 2 TMS Phage Holin (M2 Hol) Family,,,PF16081.8,84.3972,100.0 SIF-Syn: Conserved synteny of holin between lysin A and lysin B in A4 phages /note=Has two predicted transmembrane helices CDS 5926 - 6906 /gene="10" /product="gp10" /function="lysin B" /locus tag="Kremtemulon_10" /note=Original Glimmer call @bp 5926 has strength 14.21; Genemark calls start at 5926 /note=SSC: 5926-6906 CP: yes SCS: both ST: SS BLAST-Start: [lysin B [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 0.0 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.066, -4.551528886052257, no F: lysin B SIF-BLAST: ,,[lysin B [Mycobacterium phage Cintron] ],,YP_010062650,100.0,0.0 SIF-HHPRED: Gene 12 protein; alpha/beta sandwich, CELL ADHESION; 2.0A {Mycobacterium phage D29},,,3HC7_A,69.3252,100.0 SIF-Syn: Conserved synteny of lysin A, holin, lysin B as in other A4 phages CDS 6929 - 8626 /gene="11" /product="gp11" /function="terminase, large subunit" /locus tag="Kremtemulon_11" /note=Original Glimmer call @bp 6929 has strength 10.35; Genemark calls start at 6953 /note=SSC: 6929-8626 CP: yes SCS: both-gl ST: SS BLAST-Start: [terminase large subunit [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 0.0 GAP: 22 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.564, -3.50848394673489, no F: terminase, large subunit SIF-BLAST: ,,[terminase large subunit [Mycobacterium phage Cintron] ],,YP_010062651,100.0,0.0 SIF-HHPRED: Terminase large subunit; genome packaging, bacteriophage, ATPase, nuclease, VIRAL PROTEIN; HET: BR; 2.2A {Enterobacteria phage HK97},,,6Z6D_A,91.6814,100.0 SIF-Syn: CDS 8623 - 10077 /gene="12" /product="gp12" /function="portal protein" /locus tag="Kremtemulon_12" /note=Original Glimmer call @bp 8623 has strength 14.81; Genemark calls start at 8623 /note=SSC: 8623-10077 CP: yes SCS: both ST: SS BLAST-Start: [portal protein [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.169, -4.477250792015338, no F: portal protein SIF-BLAST: ,,[portal protein [Mycobacterium phage Cintron] ],,YP_010062652,100.0,0.0 SIF-HHPRED: Portal protein; Bacteriophage, SPP1, Portal Protein, Head completion proteins, Connector Complex, DNA Channel, VIRAL PROTEIN; 2.7A {Bacillus subtilis},,,7Z4W_D,90.0826,100.0 SIF-Syn: matches other A4 genomes CDS 10074 - 10934 /gene="13" /product="gp13" /function="capsid maturation protease" /locus tag="Kremtemulon_13" /note=Original Glimmer call @bp 10074 has strength 11.03; Genemark calls start at 10074 /note=SSC: 10074-10934 CP: yes SCS: both ST: SS BLAST-Start: [capsid maturation protease [Mycobacterium phage Dhanush] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.072, -3.3503726477288405, yes F: capsid maturation protease SIF-BLAST: ,,[capsid maturation protease [Mycobacterium phage Dhanush] ],,AGK87129,100.0,0.0 SIF-HHPRED: SIF-Syn: Matches annotated function in other A4 genomes /note=In same phamily and 50% amino acid similarity to D29 capsid maturation protease CDS 10987 - 11499 /gene="14" /product="gp14" /function="scaffolding protein" /locus tag="Kremtemulon_14" /note=Original Glimmer call @bp 10987 has strength 12.47; Genemark calls start at 10987 /note=SSC: 10987-11499 CP: yes SCS: both ST: SS BLAST-Start: [head scaffolding protein [Mycobacterium phage BellusTerra] ],,NCBI, q1:s1 100.0% 9.56951E-120 GAP: 52 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.996, -2.9525085049809894, yes F: scaffolding protein SIF-BLAST: ,,[head scaffolding protein [Mycobacterium phage BellusTerra] ],,YP_009005572,100.0,9.56951E-120 SIF-HHPRED: Scaffold protein; major capsid protein, HK97-like fold, scaffolding protein, procapsid, VIRUS; 3.72A {Staphylococcus phage 80alpha},,,6B0X_b,39.4118,98.4 SIF-Syn: Matches other A4 genomes CDS 11527 - 12459 /gene="15" /product="gp15" /function="major capsid protein" /locus tag="Kremtemulon_15" /note=Original Glimmer call @bp 11527 has strength 18.96; Genemark calls start at 11527 /note=SSC: 11527-12459 CP: yes SCS: both ST: SS BLAST-Start: [major capsid protein [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 0.0 GAP: 27 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.164, -4.87383788125753, no F: major capsid protein SIF-BLAST: ,,[major capsid protein [Mycobacterium phage Cintron] ],,YP_010062655,100.0,0.0 SIF-HHPRED: Major head protein; major capsid protein, HK97-like fold, scaffolding protein, procapsid, VIRUS; 3.72A {Staphylococcus phage 80alpha},,,6B0X_G,94.5161,100.0 SIF-Syn: Matches other A4 genomes CDS 12526 - 12732 /gene="16" /product="gp16" /function="hypothetical protein" /locus tag="Kremtemulon_16" /note=Original Glimmer call @bp 12526 has strength 10.39; Genemark calls start at 12526 /note=SSC: 12526-12732 CP: yes SCS: both ST: SS BLAST-Start: [head-tail connector protein [Mycobacterium phage BellusTerra] ],,NCBI, q1:s1 100.0% 4.35769E-39 GAP: 66 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.669, -3.42909898063754, yes F: hypothetical protein SIF-BLAST: ,,[head-tail connector protein [Mycobacterium phage BellusTerra] ],,YP_009005574,100.0,4.35769E-39 SIF-HHPRED: SIF-Syn: CDS 12736 - 13092 /gene="17" /product="gp17" /function="head-to-tail adaptor" /locus tag="Kremtemulon_17" /note=Original Glimmer call @bp 12736 has strength 11.01; Genemark calls start at 12736 /note=SSC: 12736-13092 CP: yes SCS: both ST: SS BLAST-Start: [head-tail adaptor Ad1 [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 1.44961E-79 GAP: 3 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.918, -2.7653702713535186, yes F: head-to-tail adaptor SIF-BLAST: ,,[head-tail adaptor Ad1 [Mycobacterium phage Peaches] ],,YP_003358720,100.0,1.44961E-79 SIF-HHPRED: Hypothetical protein yqbG; ALPHA, GFT NMR, Structural Genomics, Protein Structure Initiative, PSI, NESG, SR215, Northeast Structural Genomics Consortium, Unknown; NMR {Bacillus subtilis} SCOP: a.229.1.1,,,1XN8_A,88.1356,98.6 SIF-Syn: matches other A4 genomes CDS 13089 - 13256 /gene="18" /product="gp18" /function="hypothetical protein" /locus tag="Kremtemulon_18" /note=Original Glimmer call @bp 13089 has strength 5.46; Genemark calls start at 13089 /note=SSC: 13089-13256 CP: yes SCS: both ST: SS BLAST-Start: [head-tail connector protein [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 1.29918E-33 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.345, -6.143409454131006, no F: hypothetical protein SIF-BLAST: ,,[head-tail connector protein [Mycobacterium phage Cintron] ],,YP_010062658,100.0,1.29918E-33 SIF-HHPRED: SIF-Syn: CDS 13253 - 13615 /gene="19" /product="gp19" /function="head-to-tail stopper" /locus tag="Kremtemulon_19" /note=Original Glimmer call @bp 13253 has strength 12.69; Genemark calls start at 13253 /note=SSC: 13253-13615 CP: yes SCS: both ST: SS BLAST-Start: [head closure Hc1 [Mycobacterium phage BellusTerra] ],,NCBI, q1:s1 100.0% 4.26287E-84 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.857, -4.9912573797770525, no F: head-to-tail stopper SIF-BLAST: ,,[head closure Hc1 [Mycobacterium phage BellusTerra] ],,YP_009005577,100.0,4.26287E-84 SIF-HHPRED: Head completion protein gp16; Bacteriophage, SPP1, Portal Protein, Head completion proteins, Connector Complex, DNA Channel, VIRAL PROTEIN; 2.7A {Bacillus subtilis},,,7Z4W_1,97.5,99.2 SIF-Syn: CDS 13617 - 14009 /gene="20" /product="gp20" /function="hypothetical protein" /locus tag="Kremtemulon_20" /note=Original Glimmer call @bp 13617 has strength 10.06; Genemark calls start at 13617 /note=SSC: 13617-14009 CP: yes SCS: both ST: NA BLAST-Start: [tail completion or Neck1 protein [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 8.61316E-88 GAP: 1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.623, -3.46411222063, yes F: hypothetical protein SIF-BLAST: ,,[tail completion or Neck1 protein [Mycobacterium phage Peaches] ],,YP_003358723,100.0,8.61316E-88 SIF-HHPRED: DUF5403 ; Family of unknown function (DUF5403),,,PF17395.5,83.8462,99.9 SIF-Syn: CDS 14009 - 14446 /gene="21" /product="gp21" /function="tail terminator" /locus tag="Kremtemulon_21" /note=Original Glimmer call @bp 14009 has strength 7.97; Genemark calls start at 14009 /note=SSC: 14009-14446 CP: yes SCS: both ST: SS BLAST-Start: [tail terminator [Mycobacterium phage BellusTerra] ],,NCBI, q1:s1 100.0% 9.94339E-104 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.507, -3.979581593363423, yes F: tail terminator SIF-BLAST: ,,[tail terminator [Mycobacterium phage BellusTerra] ],,YP_009005579,100.0,9.94339E-104 SIF-HHPRED: TAIL-TO-HEAD JOINING PROTEIN GP17; VIRAL PROTEIN, VIRAL INFECTION, TAILED BACTERIOPHAGE, SIPHOVIRIDAE, SPP1, VIRAL ASSEMBLY, HEAD-TO-TAIL INTERFACE, DNA GATEKEEPER, ALLOSTERIC MECHANISM; 7.2A {BACILLUS PHAGE SPP1},,,5A21_G,91.7241,99.0 SIF-Syn: Matches other A4 genomes CDS 14436 - 15026 /gene="22" /product="gp22" /function="major tail protein" /locus tag="Kremtemulon_22" /note=Original Glimmer call @bp 14436 has strength 12.46; Genemark calls start at 14436 /note=SSC: 14436-15026 CP: no SCS: both ST: NI BLAST-Start: [major tail protein [Mycobacterium phage Eris] ],,NCBI, q1:s1 100.0% 8.81389E-140 GAP: -11 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.305, -2.305049668942183, yes F: major tail protein SIF-BLAST: ,,[major tail protein [Mycobacterium phage Eris] ],,APD17718,100.0,8.81389E-140 SIF-HHPRED: YSD1_22 major tail protein; Bacteriophage tail, helical assembly, VIRAL PROTEIN; 3.5A {Bacteriophage sp.},,,6XGR_L,99.4898,98.0 SIF-Syn: Matches other A4 phages, directly upstream of tail assembly chaperones CDS 15146 - 15517 /gene="23" /product="gp23" /function="tail assembly chaperone" /locus tag="Kremtemulon_23" /note=Original Glimmer call @bp 15146 has strength 13.48; Genemark calls start at 15146 /note=SSC: 15146-15517 CP: no SCS: both ST: SS BLAST-Start: [tail assembly chaperone [Mycobacterium phage Arturo] ],,NCBI, q1:s1 100.0% 3.04529E-80 GAP: 119 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.392, -4.395041241556842, no F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Mycobacterium phage Arturo] ],,YP_009638681,100.0,3.04529E-80 SIF-HHPRED: GP24_25 ; Mycobacteriophage tail assembly protein,,,PF17388.5,95.1219,100.0 SIF-Syn: Matches other A4 genomes as site of -1 programmed frameshift. Slippery sequence "GGGGGAA" matches that of L5 -1 frameshift with frameshift occurring at the first G, position 15,490. CDS join(15146..15490,15490..16014) /gene="24" /product="gp24" /function="tail assembly chaperone" /locus tag="Kremtemulon_24" /note= /note=SSC: 15146-16014 CP: yes SCS: neither ST: NI BLAST-Start: [tail assembly chaperone [Mycobacterium phage Arturo] ],,NCBI, q1:s1 100.0% 0.0 GAP: -372 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.392, -4.395041241556842, no F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Mycobacterium phage Arturo] ],,YP_009638680,100.0,0.0 SIF-HHPRED: SIF-Syn: Matches other A4 genomes as site of -1 programmed frameshift. Slippery sequence "GGGGGAA" matches that of L5 -1 frameshift with frameshift occurring at the first G, position 15,490. CDS 15933 - 18533 /gene="25" /product="gp25" /function="tape measure protein" /locus tag="Kremtemulon_25" /note=Original Glimmer call @bp 16074 has strength 13.05; Genemark calls start at 15933 /note=SSC: 15933-18533 CP: yes SCS: both-gm ST: SS BLAST-Start: [tape measure protein [Mycobacterium phage Iceman]],,NCBI, q1:s1 100.0% 0.0 GAP: -83 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.072, -2.523003374675015, yes F: tape measure protein SIF-BLAST: ,,[tape measure protein [Mycobacterium phage Iceman]],,UVK58780,99.7691,0.0 SIF-HHPRED: Tape Measure Protein, gp57; phage tail, tail tip, tape measure protein, VIRAL PROTEIN; 3.7A {Staphylococcus virus 80alpha},,,6V8I_BF,9.93072,99.5 SIF-Syn: Matches other A4 phages CDS 18538 - 19878 /gene="26" /product="gp26" /function="minor tail protein" /locus tag="Kremtemulon_26" /note=Original Glimmer call @bp 18538 has strength 6.92; Genemark calls start at 18538 /note=SSC: 18538-19878 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Mycobacterium phage Nyxis] ],,NCBI, q1:s1 100.0% 0.0 GAP: 4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.39, -3.9531326986493704, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Mycobacterium phage Nyxis] ],,YP_009005865,100.0,0.0 SIF-HHPRED: LACTOCOCCAL PHAGE P2 ORF15; BASEPLATE, VIRAL PROTEIN; 2.6A {LACTOCOCCUS PHAGE P2},,,2WZP_P,48.6547,99.6 SIF-Syn: Matches location of other minor tail proteins in A4 phages CDS 19875 - 21653 /gene="27" /product="gp27" /function="minor tail protein" /locus tag="Kremtemulon_27" /note=Original Glimmer call @bp 19875 has strength 15.35; Genemark calls start at 19875 /note=SSC: 19875-21653 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.163, -4.349108688274226, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Mycobacterium phage Peaches] ],,YP_003358730,100.0,0.0 SIF-HHPRED: Tail-Associated Lysin, gp59; phage tail, tail tip, tape measure protein, VIRAL PROTEIN; 3.7A {Staphylococcus virus 80alpha},,,6V8I_BE,93.0743,99.1 SIF-Syn: Matches location of minor tail proteins in other A4 phages CDS 21675 - 22121 /gene="28" /product="gp28" /function="minor tail protein" /locus tag="Kremtemulon_28" /note=Original Glimmer call @bp 21675 has strength 9.01; Genemark calls start at 21675 /note=SSC: 21675-22121 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 2.23998E-106 GAP: 21 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.198, -4.274735973818826, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Mycobacterium phage Peaches] ],,YP_003358731,100.0,2.23998E-106 SIF-HHPRED: SIF-Syn: Matches location of other minor tail proteins in A4 phages CDS 22130 - 22576 /gene="29" /product="gp29" /function="minor tail protein" /locus tag="Kremtemulon_29" /note=Original Glimmer call @bp 22181 has strength 14.57; Genemark calls start at 22169 /note=SSC: 22130-22576 CP: yes SCS: both-cs ST: SS BLAST-Start: [minor tail protein [Mycobacterium phage Medusa] ],,NCBI, q1:s1 100.0% 3.84002E-101 GAP: 8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.693, -5.414317774057158, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Mycobacterium phage Medusa] ],,AGT13301,100.0,3.84002E-101 SIF-HHPRED: SIF-Syn: Matches location of other minor tail proteins in A4 phages CDS 22566 - 22994 /gene="30" /product="gp30" /function="minor tail protein" /locus tag="Kremtemulon_30" /note=Original Glimmer call @bp 22566 has strength 12.6; Genemark calls start at 22566 /note=SSC: 22566-22994 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PEACHES_30 [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 2.26048E-101 GAP: -11 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.521, -4.5049749909507835, yes F: minor tail protein SIF-BLAST: ,,[hypothetical protein PEACHES_30 [Mycobacterium phage Peaches] ],,YP_003358733,100.0,2.26048E-101 SIF-HHPRED: SIF-Syn: Matches location of other minor tail proteins in A4 phages CDS 23008 - 24867 /gene="31" /product="gp31" /function="minor tail protein" /locus tag="Kremtemulon_31" /note=Original Glimmer call @bp 23008 has strength 12.53; Genemark calls start at 23008 /note=SSC: 23008-24867 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 0.0 GAP: 13 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.39, -4.40029072999159, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Mycobacterium phage Cintron] ],,YP_010062671,99.8384,0.0 SIF-HHPRED: SIF-Syn: Matches location of other minor tail proteins in A4 phages CDS complement (24931 - 25131) /gene="32" /product="gp32" /function="hypothetical protein" /locus tag="Kremtemulon_32" /note=Original Glimmer call @bp 25131 has strength 10.99; Genemark calls start at 25131 /note=SSC: 25131-24931 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_NYXIS_32 [Mycobacterium phage Nyxis] ],,NCBI, q1:s1 100.0% 2.84135E-37 GAP: 55 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.407, -3.9166971242758706, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_NYXIS_32 [Mycobacterium phage Nyxis] ],,YP_009005871,98.4848,2.84135E-37 SIF-HHPRED: SIF-Syn: /note=membrane protein CDS complement (25187 - 26749) /gene="33" /product="gp33" /function="serine integrase" /locus tag="Kremtemulon_33" /note=Original Glimmer call @bp 26665 has strength 14.07; Genemark calls start at 26665 /note=SSC: 26749-25187 CP: yes SCS: both-cs ST: NI BLAST-Start: [serine integrase [Mycobacterium phage DirtyDunning]],,NCBI, q1:s1 100.0% 0.0 GAP: 66 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.585, -4.565733493766995, yes F: serine integrase SIF-BLAST: ,,[serine integrase [Mycobacterium phage DirtyDunning]],,QBP29324,99.6154,0.0 SIF-HHPRED: SIF-Syn: Matches location of integrase gene in other A4 genomes /note=Related pham members vary among three different start site selections, all of which are present in Kremtemulon gene 33. Here we selected start site 26749 as it captured all coding potential and had the strongest RBS score, even though it was not the most annotated start on starterator. CDS 26816 - 27244 /gene="34" /product="gp34" /function="hypothetical protein" /locus tag="Kremtemulon_34" /note=Original Glimmer call @bp 26834 has strength 3.43; Genemark calls start at 26834 /note=SSC: 26816-27244 CP: yes SCS: both-cs ST: SS BLAST-Start: [excisionase [Mycobacterium phage Iracema64] ],,NCBI, q1:s1 100.0% 2.35288E-92 GAP: 66 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.407, -3.9776535502172963, yes F: hypothetical protein SIF-BLAST: ,,[excisionase [Mycobacterium phage Iracema64] ],,YP_009190894,100.0,2.35288E-92 SIF-HHPRED: SIF-Syn: /note=Start site selected as it is the most annotated start site and has the strongest RBS score. membrane protein CDS complement (27317 - 27796) /gene="35" /product="gp35" /function="deoxycytidylate deaminase" /locus tag="Kremtemulon_35" /note=Original Glimmer call @bp 27796 has strength 3.83; Genemark calls start at 27796 /note=SSC: 27796-27317 CP: yes SCS: both ST: SS BLAST-Start: [dCMP deaminase [Mycobacterium phage Arturo] ],,NCBI, q1:s1 100.0% 1.29804E-109 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.752, -5.351280609534597, no F: deoxycytidylate deaminase SIF-BLAST: ,,[dCMP deaminase [Mycobacterium phage Arturo] ],,YP_009638692,99.3711,1.29804E-109 SIF-HHPRED: DEOXYCYTIDYLATE DEAMINASE; PYRIMIDINE METABOLISM, NUCLEOTIDE BIOSYNTHESIS, ZINC, HEXAMER, HYDROLASE, METAL-BINDING, PHOSPHOPROTEIN, ALLOSTERIC ENZYME; 2.1A {HOMO SAPIENS} SCOP: c.97.1.0,,,2W4L_E,79.2453,99.8 SIF-Syn: Matches other A4 phages (e.g., Arturo and BellusTerra) CDS complement (27793 - 27912) /gene="36" /product="gp36" /function="hypothetical protein" /locus tag="Kremtemulon_36" /note=Original Glimmer call @bp 27912 has strength 4.88; Genemark calls start at 27912 /note=SSC: 27912-27793 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein KIY68_gp56 [Mycobacterium phage Stasia] ],,NCBI, q1:s1 100.0% 2.55748E-18 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.249, -6.917343366198017, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein KIY68_gp56 [Mycobacterium phage Stasia] ],,YP_010062424,100.0,2.55748E-18 SIF-HHPRED: SIF-Syn: CDS complement (27913 - 28065) /gene="37" /product="gp37" /function="hypothetical protein" /locus tag="Kremtemulon_37" /note=Original Glimmer call @bp 28065 has strength 14.0; Genemark calls start at 28065 /note=SSC: 28065-27913 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_NYXIS_37 [Mycobacterium phage Nyxis] ],,NCBI, q1:s1 100.0% 4.14642E-26 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.055, -3.005970110175159, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_NYXIS_37 [Mycobacterium phage Nyxis] ],,YP_009005876,100.0,4.14642E-26 SIF-HHPRED: DUF4999 ; Domain of unknown function,,,PF16390.8,68.0,94.1 SIF-Syn: CDS complement (28062 - 28328) /gene="38" /product="gp38" /function="hypothetical protein" /locus tag="Kremtemulon_38" /note=Original Glimmer call @bp 28328 has strength 7.22; Genemark calls start at 28328 /note=SSC: 28328-28062 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_KRATARK_38 [Mycobacterium phage Kratark] ],,NCBI, q1:s1 100.0% 1.74024E-57 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.094, -4.635604911695725, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_KRATARK_38 [Mycobacterium phage Kratark] ],,APM00072,100.0,1.74024E-57 SIF-HHPRED: SIF-Syn: CDS complement (28325 - 28411) /gene="39" /product="gp39" /function="hypothetical protein" /locus tag="Kremtemulon_39" /note= /note=SSC: 28411-28325 CP: yes SCS: neither ST: NI BLAST-Start: [hypothetical protein SEA_IRACEMA64_40 [Mycobacterium phage Iracema64] ],,NCBI, q1:s5 100.0% 6.09928E-8 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.327, -4.005585920014169, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_IRACEMA64_40 [Mycobacterium phage Iracema64] ],,YP_009190899,87.5,6.09928E-8 SIF-HHPRED: SIF-Syn: /note=membrane protein CDS complement (28408 - 28665) /gene="40" /product="gp40" /function="helix-turn-helix DNA binding domain" /locus tag="Kremtemulon_40" /note=Original Glimmer call @bp 28665 has strength 11.56; Genemark calls start at 28665 /note=SSC: 28665-28408 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PEACHES_40 [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 5.75576E-55 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.209, -2.297359520375101, yes F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[hypothetical protein PEACHES_40 [Mycobacterium phage Peaches] ],,YP_003358743,100.0,5.75576E-55 SIF-HHPRED: Transcriptional regulator, MarR family; YP_013417.1, multiple antibiotic-resistance repressor (MarR), Structural Genomics, Joint Center for Structural Genomics, JCSG, Protein Structure Initiative; HET: MSE; 2.07A {Listeria monocytogenes str. 4b},,,2QWW_H,63.5294,98.5 SIF-Syn: /note=HHPRED alignments show match up of 3 alpha helices separated by CDS complement (28662 - 29018) /gene="41" /product="gp41" /function="hypothetical protein" /locus tag="Kremtemulon_41" /note=Original Glimmer call @bp 29018 has strength 11.96; Genemark calls start at 29018 /note=SSC: 29018-28662 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PEACHES_41 [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 4.23896E-80 GAP: -14 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.564, -3.5707972674952195, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PEACHES_41 [Mycobacterium phage Peaches] ],,YP_003358744,100.0,4.23896E-80 SIF-HHPRED: SIF-Syn: CDS complement (29005 - 29280) /gene="42" /product="gp42" /function="hypothetical protein" /locus tag="Kremtemulon_42" /note=Original Glimmer call @bp 29280 has strength 11.24; Genemark calls start at 29280 /note=SSC: 29280-29005 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein CH07_gp43 [Mycobacterium phage BellusTerra] ],,NCBI, q1:s1 100.0% 7.34807E-59 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.329, -4.142194467337367, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein CH07_gp43 [Mycobacterium phage BellusTerra] ],,YP_009005601,100.0,7.34807E-59 SIF-HHPRED: SIF-Syn: CDS complement (29277 - 29510) /gene="43" /product="gp43" /function="hypothetical protein" /locus tag="Kremtemulon_43" /note=Original Glimmer call @bp 29510 has strength 12.5; Genemark calls start at 29510 /note=SSC: 29510-29277 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein KIY71_gp42 [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 2.4145E-46 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.74, -3.2804507689989406, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein KIY71_gp42 [Mycobacterium phage Cintron] ],,YP_010062682,100.0,2.4145E-46 SIF-HHPRED: SIF-Syn: CDS complement (29507 - 29674) /gene="44" /product="gp44" /function="hypothetical protein" /locus tag="Kremtemulon_44" /note=Original Glimmer call @bp 29674 has strength 15.73; Genemark calls start at 29674 /note=SSC: 29674-29507 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein KIY71_gp43 [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 9.63313E-30 GAP: 20 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.072, -2.523003374675015, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein KIY71_gp43 [Mycobacterium phage Cintron] ],,YP_010062683,100.0,9.63313E-30 SIF-HHPRED: SIF-Syn: CDS complement (29695 - 31521) /gene="45" /product="gp45" /function="DNA polymerase I" /locus tag="Kremtemulon_45" /note=Original Glimmer call @bp 31521 has strength 15.66; Genemark calls start at 31521 /note=SSC: 31521-29695 CP: yes SCS: both ST: SS BLAST-Start: [DNA polymerase [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 0.0 GAP: 7 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.407, -4.36385515561809, no F: DNA polymerase I SIF-BLAST: ,,[DNA polymerase [Mycobacterium phage Cintron] ],,YP_010062684,100.0,0.0 SIF-HHPRED: DNA polymerase I; phiVC8, PolA, DpoZ, thumb-exo open, thumb-exo closed, VIRAL PROTEIN; 2.8A {Vibrio phage phiVC8},,,7PBK_B,95.3947,100.0 SIF-Syn: CDS complement (31529 - 31831) /gene="46" /product="gp46" /function="helix-turn-helix DNA binding domain" /locus tag="Kremtemulon_46" /note=Original Glimmer call @bp 31831 has strength 10.41; Genemark calls start at 31831 /note=SSC: 31831-31529 CP: yes SCS: both ST: SS BLAST-Start: [HTH DNA binding protein [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 6.60175E-64 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.478, -3.8294249377118383, yes F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[HTH DNA binding protein [Mycobacterium phage Cintron] ],,YP_010062685,100.0,6.60175E-64 SIF-HHPRED: DNA-invertase; helix-turn-helix, site-specific recombinase, RECOMBINATION; 3.51A {Enterobacteria phage Mu},,,3UJ3_X,85.0,98.2 SIF-Syn: /note=Aligns with several HTH-domain proteins on HHPRED and contains a stretch of 3 predicted helices separated by short turns of 3 aa CDS complement (31831 - 32004) /gene="47" /product="gp47" /function="hypothetical protein" /locus tag="Kremtemulon_47" /note=Original Glimmer call @bp 32004 has strength 16.58; Genemark calls start at 31983 /note=SSC: 32004-31831 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein PEACHES_47 [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 3.80614E-32 GAP: 8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.141, -5.444803624783377, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PEACHES_47 [Mycobacterium phage Peaches] ],,YP_003358750,100.0,3.80614E-32 SIF-HHPRED: SIF-Syn: /note=Has strong HHPRED hits to `putative lipoproteins`; however, only a small number of A4 phages have called it such. Seems like weak evidence to call the lipoprotein function so left as NKF. CDS complement (32013 - 32732) /gene="48" /product="gp48" /function="ThyX-like thymidylate synthase" /locus tag="Kremtemulon_48" /note=Original Glimmer call @bp 32732 has strength 11.6; Genemark calls start at 32732 /note=SSC: 32732-32013 CP: yes SCS: both ST: SS BLAST-Start: [thymidylate synthase [Mycobacterium phage BellusTerra] ],,NCBI, q1:s1 100.0% 1.45181E-174 GAP: 67 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.055, -3.005970110175159, yes F: ThyX-like thymidylate synthase SIF-BLAST: ,,[thymidylate synthase [Mycobacterium phage BellusTerra] ],,YP_009005607,100.0,1.45181E-174 SIF-HHPRED: Thymidylate synthase thyX; ThyX, FAD, FdUMP, Flavoprotein, Methyltransferase, Nucleotide biosynthesis, Transferase, Structural Genomics, Seattle Structural Genomics Center for Infectious; HET: UFP, FAD; 1.9A {Mycobacterium tuberculosis},,,3GWC_B,98.3264,100.0 SIF-Syn: Matches functional call at same locus in other A4 phages CDS complement (32800 - 33336) /gene="49" /product="gp49" /function="hypothetical protein" /locus tag="Kremtemulon_49" /note=Original Glimmer call @bp 33336 has strength 8.47; Genemark calls start at 33336 /note=SSC: 33336-32800 CP: yes SCS: both ST: SS BLAST-Start: [ribonucleoside reductase class II [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 2.14265E-119 GAP: 26 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.777, -3.061661148509975, yes F: hypothetical protein SIF-BLAST: ,,[ribonucleoside reductase class II [Mycobacterium phage Cintron] ],,YP_010062688,100.0,2.14265E-119 SIF-HHPRED: SIF-Syn: /note=This gene does not have the most annotated start for this pham; however, of the start sites available, the most annotated one was selected. CDS complement (33363 - 35396) /gene="50" /product="gp50" /function="ribonucleotide reductase" /locus tag="Kremtemulon_50" /note=Original Glimmer call @bp 35396 has strength 15.56; Genemark calls start at 35396 /note=SSC: 35396-33363 CP: yes SCS: both ST: SS BLAST-Start: [ribonucleotide reductase [Mycobacterium phage Iracema64] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.295, -4.599515895103776, no F: ribonucleotide reductase SIF-BLAST: ,,[ribonucleotide reductase [Mycobacterium phage Iracema64] ],,YP_009190910,99.8523,0.0 SIF-HHPRED: RIBONUCLEOSIDE TRIPHOSPHATE REDUCTASE; 10-stranded alpha-beta barrel, central finger loop, OXIDOREDUCTASE; 1.75A {Lactobacillus leichmannii} SCOP: c.7.1.4,,,1L1L_C,99.4092,100.0 SIF-Syn: CDS complement (35393 - 36289) /gene="51" /product="gp51" /function="DNA binding protein" /locus tag="Kremtemulon_51" /note=Original Glimmer call @bp 36289 has strength 13.74; Genemark calls start at 36289 /note=SSC: 36289-35393 CP: yes SCS: both ST: SS BLAST-Start: [sigma-K factor [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 0.0 GAP: 3 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.006, -4.679270584912496, no F: DNA binding protein SIF-BLAST: ,,[sigma-K factor [Mycobacterium phage Peaches] ],,YP_003358754,100.0,0.0 SIF-HHPRED: ECF RNA polymerase sigma factor SigK; sigma factor, transcription initiation, DNA binding, Promoter DNA binding and transcription initiation, anti-sigma factor, DNA BINDING; HET: CD; 2.4A {Mycobacterium tuberculosis},,,4NQW_A,50.3356,99.2 SIF-Syn: /note=HHPRED hits to sigma factors (DNA binding proteins) but could not identify obvious helix-turn-helix domain so left as more general DNA binding function. CDS complement (36293 - 37060) /gene="52" /product="gp52" /function="metallophosphatase" /locus tag="Kremtemulon_52" /note=Original Glimmer call @bp 37060 has strength 15.32; Genemark calls start at 37060 /note=SSC: 37060-36293 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PEACHES_52 [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 0.0 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.055, -2.5410833118725082, yes F: metallophosphatase SIF-BLAST: ,,[hypothetical protein PEACHES_52 [Mycobacterium phage Peaches] ],,YP_003358755,100.0,0.0 SIF-HHPRED: d.159.1.7 (A:4-196) Vacuolar protein sorting 29, VPS29 {Cryptosporidium parvum [TaxId: 5807]} | CLASS: Alpha and beta proteins (a+b), FOLD: Metallo-dependent phosphatases, SUPFAM: Metallo-dependent phosphatases, FAM: YfcE-like,,,SCOP_d2a22a1,97.6471,99.9 SIF-Syn: Using updated term metallophosphatase as opposed to metallophosphoesterase /note=Has HExxH motif CDS complement (37053 - 37328) /gene="53" /product="gp53" /function="hypothetical protein" /locus tag="Kremtemulon_53" /note=Original Glimmer call @bp 37328 has strength 13.52; Genemark calls start at 37328 /note=SSC: 37328-37053 CP: yes SCS: both ST: SS BLAST-Start: [phosphofructokinase [Mycobacterium phage Cindaradix] ],,NCBI, q1:s16 100.0% 9.06926E-61 GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.072, -2.442961286954254, yes F: hypothetical protein SIF-BLAST: ,,[phosphofructokinase [Mycobacterium phage Cindaradix] ],,YP_010063305,85.8491,9.06926E-61 SIF-HHPRED: SIF-Syn: CDS complement (37321 - 37431) /gene="54" /product="gp54" /function="hypothetical protein" /locus tag="Kremtemulon_54" /note=Genemark calls start at 37431 /note=SSC: 37431-37321 CP: yes SCS: genemark ST: SS BLAST-Start: [hypothetical protein TIROTHETA9_54 [Mycobacterium phage TiroTheta9]],,NCBI, q1:s68 100.0% 4.98455E-18 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.005, -3.4105598537121566, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein TIROTHETA9_54 [Mycobacterium phage TiroTheta9]],,AEM05954,34.9515,4.98455E-18 SIF-HHPRED: SIF-Syn: CDS complement (37428 - 38087) /gene="55" /product="gp55" /function="DNA primase" /locus tag="Kremtemulon_55" /note=Original Glimmer call @bp 38087 has strength 12.01; Genemark calls start at 38087 /note=SSC: 38087-37428 CP: yes SCS: both ST: SS BLAST-Start: [DNA primase [Mycobacterium phage BellusTerra] ],,NCBI, q1:s1 100.0% 1.26298E-160 GAP: -185 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.151, -2.338663114094478, yes F: DNA primase SIF-BLAST: ,,[DNA primase [Mycobacterium phage BellusTerra] ],,YP_009005614,100.0,1.26298E-160 SIF-HHPRED: DNA primase; structural genomics, DNA-binding, primase, truncation, Seattle Structural Genomics Center for Infectious Disease, SSGCID, TRANSFERASE; HET: SO4; 2.4A {Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)} SCOP: e.13.1.0,,,5VAZ_A,96.8036,99.8 SIF-Syn: CDS complement (37903 - 38316) /gene="56" /product="gp56" /function="DNA primase" /locus tag="Kremtemulon_56" /note= /note=SSC: 38316-37903 CP: yes SCS: neither ST: NI BLAST-Start: [DNA primase [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 2.94051E-95 GAP: -17 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.437, -4.4261092987867166, yes F: DNA primase SIF-BLAST: ,,[DNA primase [Mycobacterium phage Cintron] ],,YP_010062695,99.2701,2.94051E-95 SIF-HHPRED: DNA primase; phage, complex, helicase, REPLICATION-DNA-RNA complex; HET: GTP; 3.9A {Escherichia phage T4},,,8DWJ_A,41.6058,98.1 SIF-Syn: Overlapping DNA primase genes matches other A4 phages CDS complement (38300 - 38434) /gene="57" /product="gp57" /function="hypothetical protein" /locus tag="Kremtemulon_57" /note=Original Glimmer call @bp 38434 has strength 22.05; Genemark calls start at 38434 /note=SSC: 38434-38300 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PEACHES_56 [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 5.0024E-22 GAP: 21 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.305, -2.4811409279978642, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PEACHES_56 [Mycobacterium phage Peaches] ],,YP_003358759,100.0,5.0024E-22 SIF-HHPRED: SIF-Syn: CDS complement (38456 - 38926) /gene="58" /product="gp58" /function="endonuclease VII" /locus tag="Kremtemulon_58" /note=Original Glimmer call @bp 38926 has strength 5.87; Genemark calls start at 38926 /note=SSC: 38926-38456 CP: yes SCS: both ST: SS BLAST-Start: [endonuclease VII [Mycobacterium phage Iracema64] ],,NCBI, q1:s1 100.0% 1.51484E-110 GAP: 63 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.65, -3.389881189012514, no F: endonuclease VII SIF-BLAST: ,,[endonuclease VII [Mycobacterium phage Iracema64] ],,YP_009190918,100.0,1.51484E-110 SIF-HHPRED: RECOMBINATION ENDONUCLEASE VII; ENDONUCLEASE, RESOLVASE, HOLLIDAY JUNCTION, DNASE; HET: SO4; 1.32A {BACTERIOPHAGE T4} SCOP: d.4.1.5, a.140.4.1,,,1E7L_A,48.0769,99.6 SIF-Syn: Matches other A4 phages CDS complement (38990 - 39814) /gene="59" /product="gp59" /function="hydrolase" /locus tag="Kremtemulon_59" /note=Original Glimmer call @bp 39808 has strength 13.19; Genemark calls start at 39808 /note=SSC: 39814-38990 CP: yes SCS: both-cs ST: SS BLAST-Start: [esterase/lipase [Mycobacterium phage Iracema64] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.222, -4.878091415897789, no F: hydrolase SIF-BLAST: ,,[esterase/lipase [Mycobacterium phage Iracema64] ],,YP_009190920,99.2701,0.0 SIF-HHPRED: Alpha/beta hydrolase fold protein; structural genomics, APC103277, alpha/beta hydrolase fold protein, PSI-Biology, Midwest Center for Structural Genomics, MCSG, HYDROLASE; HET: MSE; 1.48A {Chitinophaga pinensis} SCOP: c.69.1.0,,,4PW0_A,95.9854,100.0 SIF-Syn: CDS complement (39811 - 40191) /gene="60" /product="gp60" /function="hypothetical protein" /locus tag="Kremtemulon_60" /note=Original Glimmer call @bp 40182 has strength 13.39; Genemark calls start at 40182 /note=SSC: 40191-39811 CP: yes SCS: both-cs ST: SS BLAST-Start: [hypothetical protein KIY71_gp60 [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 5.51538E-88 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.021, -4.997292723641609, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein KIY71_gp60 [Mycobacterium phage Cintron] ],,YP_010062700,99.2064,5.51538E-88 SIF-HHPRED: SIF-Syn: /note=Has strong HHPRED hit to DrpA-like dna processing chain A. However, more recent A4 annotations left this as NKF. CDS complement (40188 - 40349) /gene="61" /product="gp61" /function="hypothetical protein" /locus tag="Kremtemulon_61" /note=Original Glimmer call @bp 40349 has strength 7.92; Genemark calls start at 40358 /note=SSC: 40349-40188 CP: yes SCS: both-gl ST: NA BLAST-Start: GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.936, -4.825272756372522, no F: hypothetical protein SIF-BLAST: SIF-HHPRED: SIF-Syn: Orpham CDS complement (40346 - 40570) /gene="62" /product="gp62" /function="hypothetical protein" /locus tag="Kremtemulon_62" /note=Original Glimmer call @bp 40570 has strength 14.45; Genemark calls start at 40570 /note=SSC: 40570-40346 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein KIY78_gp57 [Mycobacterium phage Cindaradix] ],,NCBI, q1:s1 100.0% 4.51424E-45 GAP: 57 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.209, -2.156361006712914, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein KIY78_gp57 [Mycobacterium phage Cindaradix] ],,YP_010063312,100.0,4.51424E-45 SIF-HHPRED: SIF-Syn: /note=Does not have most annotated start site for the pham, but we selected the start site most often annotated from the available choices. Whichever start selected will have a gap, and so the selected start also has strongest-scoring RBS. CDS complement (40628 - 41164) /gene="63" /product="gp63" /function="phosphoribosyltransferase" /locus tag="Kremtemulon_63" /note=Original Glimmer call @bp 41164 has strength 16.31; Genemark calls start at 41164 /note=SSC: 41164-40628 CP: yes SCS: both ST: SS BLAST-Start: [phosphoribosyl transferase [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 5.65589E-128 GAP: 19 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.198, -4.926874829490106, no F: phosphoribosyltransferase SIF-BLAST: ,,[phosphoribosyl transferase [Mycobacterium phage Peaches] ],,YP_003358764,100.0,5.65589E-128 SIF-HHPRED: Purine phosphoribosyltransferase (GpT-1); Sulfolobus, solfataricus, adenine, phosphoribosyltransferase, transferase; HET: PO4; 2.4A {Sulfolobus solfataricus},,,4TRB_A,75.2809,99.5 SIF-Syn: CDS complement (41184 - 41990) /gene="64" /product="gp64" /function="DnaB-like dsDNA helicase" /locus tag="Kremtemulon_64" /note=Original Glimmer call @bp 41990 has strength 13.44; Genemark calls start at 41990 /note=SSC: 41990-41184 CP: yes SCS: both ST: SS BLAST-Start: [DnaB-like replicative helicase [Mycobacterium phage BellusTerra] ],,NCBI, q1:s1 100.0% 0.0 GAP: 84 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.174, -4.85287563363746, no F: DnaB-like dsDNA helicase SIF-BLAST: ,,[DnaB-like replicative helicase [Mycobacterium phage BellusTerra] ],,YP_009005623,100.0,0.0 SIF-HHPRED: Replicative DNA helicase; Helicase, DnaB, Helicase loader protein, DnaC, Structural Genomics, REPLICATION, HYDROLASE; 3.1A {Escherichia coli},,,6KZA_B,94.403,99.9 SIF-Syn: CDS complement (42075 - 42269) /gene="65" /product="gp65" /function="hypothetical protein" /locus tag="Kremtemulon_65" /note=Original Glimmer call @bp 42269 has strength 13.32; Genemark calls start at 42269 /note=SSC: 42269-42075 CP: yes SCS: both ST: SS BLAST-Start: [DnaB-like helicase [Mycobacterium phage Arturo] ],,NCBI, q1:s1 100.0% 4.08855E-40 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.209, -2.2364030944336752, yes F: hypothetical protein SIF-BLAST: ,,[DnaB-like helicase [Mycobacterium phage Arturo] ],,YP_009638721,100.0,4.08855E-40 SIF-HHPRED: SIF-Syn: CDS complement (42266 - 42490) /gene="66" /product="gp66" /function="hypothetical protein" /locus tag="Kremtemulon_66" /note=Original Glimmer call @bp 42490 has strength 8.36; Genemark calls start at 42490 /note=SSC: 42490-42266 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein KIY71_gp67 [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 1.35232E-47 GAP: 9 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.54, -5.734826123116989, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein KIY71_gp67 [Mycobacterium phage Cintron] ],,YP_010062707,100.0,1.35232E-47 SIF-HHPRED: SIF-Syn: CDS complement (42500 - 43291) /gene="67" /product="gp67" /function="Cas4 exonuclease" /locus tag="Kremtemulon_67" /note=Original Glimmer call @bp 43291 has strength 13.28; Genemark calls start at 43291 /note=SSC: 43291-42500 CP: yes SCS: both ST: SS BLAST-Start: [exonuclease [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.187, -4.379101430921652, yes F: Cas4 exonuclease SIF-BLAST: ,,[exonuclease [Mycobacterium phage Peaches] ],,YP_003358769,100.0,0.0 SIF-HHPRED: CRISPR-associated exonuclease Cas4; CRISPR Cas adaptation type I-C, HYDROLASE-DNA complex; 4.26A {Alkalihalobacillus halodurans C-125},,,8D3P_I,99.2395,99.9 SIF-Syn: CDS complement (43288 - 43710) /gene="68" /product="gp68" /function="hypothetical protein" /locus tag="Kremtemulon_68" /note=Original Glimmer call @bp 43710 has strength 5.54; Genemark calls start at 43710 /note=SSC: 43710-43288 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein KIY71_gp69 [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 2.01253E-96 GAP: 81 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.636, -5.51667142252997, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein KIY71_gp69 [Mycobacterium phage Cintron] ],,YP_010062709,100.0,2.01253E-96 SIF-HHPRED: SIF-Syn: CDS complement (43792 - 44304) /gene="69" /product="gp69" /function="immunity repressor" /locus tag="Kremtemulon_69" /note=Original Glimmer call @bp 44304 has strength 9.99; Genemark calls start at 44304 /note=SSC: 44304-43792 CP: yes SCS: both ST: SS BLAST-Start: [transcriptional repressor [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 9.54928E-121 GAP: 210 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.721, -3.5290106867422555, no F: immunity repressor SIF-BLAST: ,,[transcriptional repressor [Mycobacterium phage Cintron] ],,YP_010062710,100.0,9.54928E-121 SIF-HHPRED: Immunity repressor; Immunity repressor, helix-turn-helix motif, DNA binding protein, GENE REGULATION, GENE REGULATION-DNA complex; HET: MSE; 2.79A {Mycobacterium phage TipsytheTRex},,,7TZ1_A,95.2941,100.0 SIF-Syn: CDS complement (44515 - 44643) /gene="70" /product="gp70" /function="hypothetical protein" /locus tag="Kremtemulon_70" /note=Original Glimmer call @bp 44643 has strength 12.85; Genemark calls start at 44649 /note=SSC: 44643-44515 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein SEA_IRACEMA64_71 [Mycobacterium phage Iracema64] ],,NCBI, q1:s37 100.0% 5.45804E-19 GAP: 121 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.27, -4.125189544139167, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_IRACEMA64_71 [Mycobacterium phage Iracema64] ],,YP_009190930,53.8462,5.45804E-19 SIF-HHPRED: SIF-Syn: CDS complement (44765 - 45013) /gene="71" /product="gp71" /function="hypothetical protein" /locus tag="Kremtemulon_71" /note=Original Glimmer call @bp 45013 has strength 7.83; Genemark calls start at 45013 /note=SSC: 45013-44765 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein KIY71_gp73 [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 4.14076E-53 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.486, -3.75071250087134, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein KIY71_gp73 [Mycobacterium phage Cintron] ],,YP_010062713,100.0,4.14076E-53 SIF-HHPRED: SIF-Syn: CDS complement (45010 - 45324) /gene="72" /product="gp72" /function="hypothetical protein" /locus tag="Kremtemulon_72" /note=Original Glimmer call @bp 45324 has strength 14.13; Genemark calls start at 45324 /note=SSC: 45324-45010 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PEACHES_71 [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 3.35322E-69 GAP: 16 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.072, -2.523003374675015, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PEACHES_71 [Mycobacterium phage Peaches] ],,YP_003358774,100.0,3.35322E-69 SIF-HHPRED: SIF-Syn: CDS complement (45341 - 45481) /gene="73" /product="gp73" /function="hypothetical protein" /locus tag="Kremtemulon_73" /note=Original Glimmer call @bp 45481 has strength 0.44 /note=SSC: 45481-45341 CP: yes SCS: glimmer ST: SS BLAST-Start: [hypothetical protein PBI_NYXIS_73 [Mycobacterium phage Nyxis] ],,NCBI, q1:s1 100.0% 3.02893E-23 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.28, -4.104706087827342, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_NYXIS_73 [Mycobacterium phage Nyxis] ],,YP_009005912,100.0,3.02893E-23 SIF-HHPRED: SIF-Syn: CDS complement (45481 - 45795) /gene="74" /product="gp74" /function="hypothetical protein" /locus tag="Kremtemulon_74" /note=Original Glimmer call @bp 45795 has strength 7.98; Genemark calls start at 45777 /note=SSC: 45795-45481 CP: yes SCS: both-gl ST: SS BLAST-Start: [site-specific recombination directionality factor RDF [Mycobacterium phage Camperdownii] ],,NCBI, q1:s1 100.0% 3.82546E-69 GAP: 248 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.711, -4.106817306804867, yes F: hypothetical protein SIF-BLAST: ,,[site-specific recombination directionality factor RDF [Mycobacterium phage Camperdownii] ],,YP_010062889,100.0,3.82546E-69 SIF-HHPRED: SIF-Syn: /note=Starterator shows this gene does not have the most annotated start in the pham. From the available starts, chose the one selected most often. Double start, selected the second based on mass spec data. CDS complement (46044 - 46628) /gene="75" /product="gp75" /function="DNA methyltransferase" /locus tag="Kremtemulon_75" /note=Original Glimmer call @bp 46631 has strength 7.43; Genemark calls start at 46628 /note=SSC: 46628-46044 CP: yes SCS: both-gm ST: SS BLAST-Start: [DNA methyltransferase [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 8.38196E-137 GAP: -23 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.931, -5.186767100221219, no F: DNA methyltransferase SIF-BLAST: ,,[DNA methyltransferase [Mycobacterium phage Peaches] ],,YP_003358779,100.0,8.38196E-137 SIF-HHPRED: c.66.1.26 (A:) DNA methylase HaeIII {Haemophilus aegyptius [TaxId: 197575]},,,1G55_A,31.4433,93.5 SIF-Syn: CDS complement (46606 - 47139) /gene="76" /product="gp76" /function="DNA methyltransferase" /locus tag="Kremtemulon_76" /note=Original Glimmer call @bp 47139 has strength 6.93; Genemark calls start at 47139 /note=SSC: 47139-46606 CP: yes SCS: both ST: SS BLAST-Start: [DNA methyltransferase [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 9.98411E-124 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.312, -4.943889544421484, no F: DNA methyltransferase SIF-BLAST: ,,[DNA methyltransferase [Mycobacterium phage Peaches] ],,YP_003358780,100.0,9.98411E-124 SIF-HHPRED: Modification methylase HhaI; CG-SPECIFICITY, CPG SEQUENCE, C5-METHYLCYTOSINE, NUCLEOTIDE FLIPPING, S-ADENOSYL-L-HOMOCYSTEINE, COMPLEX (METHYLTRANSFERASE- DNA), transferase-DNA complex; HET: SO4, 3DR, SAH; 1.594A {Haemophilus parahaemolyticus},,,5CIY_A,98.3051,100.0 SIF-Syn: CDS complement (47136 - 47306) /gene="77" /product="gp77" /function="hypothetical protein" /locus tag="Kremtemulon_77" /note=Original Glimmer call @bp 47306 has strength 13.6; Genemark calls start at 47306 /note=SSC: 47306-47136 CP: yes SCS: both ST: SS BLAST-Start: [HNH endonuclease [Mycobacterium phage Peaches] ],,NCBI, q1:s1 100.0% 1.17778E-31 GAP: 2 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.42, -4.3372389842445, no F: hypothetical protein SIF-BLAST: ,,[HNH endonuclease [Mycobacterium phage Peaches] ],,YP_003358781,100.0,1.17778E-31 SIF-HHPRED: SIF-Syn: CDS complement (47309 - 47779) /gene="78" /product="gp78" /function="SprT-like protease" /locus tag="Kremtemulon_78" /note=Original Glimmer call @bp 47779 has strength 7.42; Genemark calls start at 47779 /note=SSC: 47779-47309 CP: yes SCS: both ST: SS BLAST-Start: [SprT-like protease [Mycobacterium phage ICleared] ],,NCBI, q1:s1 100.0% 2.7337E-111 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.554, -4.631219452820914, yes F: SprT-like protease SIF-BLAST: ,,[SprT-like protease [Mycobacterium phage ICleared] ],,YP_010062983,98.7179,2.7337E-111 SIF-HHPRED: SprT-like domain-containing protein Spartan; DPC repair protease, DNA BINDING PROTEIN; HET: ADP, MLZ, FLC; 1.5A {Homo sapiens},,,6MDW_A,85.2564,99.8 SIF-Syn: CDS complement (47776 - 47946) /gene="79" /product="gp79" /function="hypothetical protein" /locus tag="Kremtemulon_79" /note=Original Glimmer call @bp 47922 has strength 3.44; Genemark calls start at 47922 /note=SSC: 47946-47776 CP: yes SCS: both-cs ST: SS BLAST-Start: [hypothetical protein PBI_KAMPY_81 [Mycobacterium phage Kampy] ],,NCBI, q1:s1 100.0% 3.21115E-30 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 0.874, -7.1313160653937935, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_KAMPY_81 [Mycobacterium phage Kampy] ],,YP_009031941,98.2143,3.21115E-30 SIF-HHPRED: SIF-Syn: CDS complement (47943 - 48296) /gene="80" /product="gp80" /function="hypothetical protein" /locus tag="Kremtemulon_80" /note=Original Glimmer call @bp 48296 has strength 12.44; Genemark calls start at 48296 /note=SSC: 48296-47943 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_KRATARK_82 [Mycobacterium phage Kratark] ],,NCBI, q1:s1 100.0% 7.77266E-80 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.564, -4.16062280240617, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_KRATARK_82 [Mycobacterium phage Kratark] ],,APM00116,100.0,7.77266E-80 SIF-HHPRED: SIF-Syn: CDS complement (48293 - 48469) /gene="81" /product="gp81" /function="hypothetical protein" /locus tag="Kremtemulon_81" /note=Original Glimmer call @bp 48469 has strength 14.61; Genemark calls start at 48469 /note=SSC: 48469-48293 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein KIY71_gp84 [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 2.70094E-33 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.39, -4.40029072999159, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein KIY71_gp84 [Mycobacterium phage Cintron] ],,YP_010062724,100.0,2.70094E-33 SIF-HHPRED: SIF-Syn: CDS complement (48466 - 48636) /gene="82" /product="gp82" /function="hypothetical protein" /locus tag="Kremtemulon_82" /note=Original Glimmer call @bp 48636 has strength 8.75; Genemark calls start at 48636 /note=SSC: 48636-48466 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein KIY71_gp85 [Mycobacterium phage Cintron] ],,NCBI, q4:s1 94.6429% 5.18685E-24 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.564, -3.588526034455651, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein KIY71_gp85 [Mycobacterium phage Cintron] ],,YP_010062725,89.2857,5.18685E-24 SIF-HHPRED: SIF-Syn: CDS complement (48633 - 48731) /gene="83" /product="gp83" /function="hypothetical protein" /locus tag="Kremtemulon_83" /note=Genemark calls start at 48731 /note=SSC: 48731-48633 CP: yes SCS: genemark ST: SS BLAST-Start: [hypothetical protein CH07_gp86 [Mycobacterium phage BellusTerra] ],,NCBI, q1:s1 100.0% 3.40609E-14 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.521, -3.6776057178969577, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein CH07_gp86 [Mycobacterium phage BellusTerra] ],,YP_009005644,100.0,3.40609E-14 SIF-HHPRED: SIF-Syn: /note=Two tandem starts; here went with most annotated start on Starterator CDS complement (48731 - 48937) /gene="84" /product="gp84" /function="hypothetical protein" /locus tag="Kremtemulon_84" /note=Original Glimmer call @bp 48937 has strength 11.97; Genemark calls start at 48937 /note=SSC: 48937-48731 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein KIY71_gp87 [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 8.86186E-40 GAP: 22 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.67, -3.812150084159046, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein KIY71_gp87 [Mycobacterium phage Cintron] ],,YP_010062727,100.0,8.86186E-40 SIF-HHPRED: SIF-Syn: CDS complement (48960 - 49844) /gene="85" /product="gp85" /function="hypothetical protein" /locus tag="Kremtemulon_85" /note=Original Glimmer call @bp 49844 has strength 12.01; Genemark calls start at 49844 /note=SSC: 49844-48960 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein KIY71_gp88 [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 0.0 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.808, -3.077027835973012, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein KIY71_gp88 [Mycobacterium phage Cintron] ],,YP_010062728,100.0,0.0 SIF-HHPRED: SIF-Syn: /note=Two tandem starts; here went with most annotated start on Starterator CDS complement (49844 - 50071) /gene="86" /product="gp86" /function="hypothetical protein" /locus tag="Kremtemulon_86" /note=Original Glimmer call @bp 50071 has strength 15.55; Genemark calls start at 50071 /note=SSC: 50071-49844 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein KIY71_gp89 [Mycobacterium phage Cintron] ],,NCBI, q1:s1 100.0% 6.232E-48 GAP: 28 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.045, -5.12434507728107, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein KIY71_gp89 [Mycobacterium phage Cintron] ],,YP_010062729,100.0,6.232E-48 SIF-HHPRED: SIF-Syn: CDS complement (50100 - 50219) /gene="87" /product="gp87" /function="hypothetical protein" /locus tag="Kremtemulon_87" /note=Original Glimmer call @bp 50219 has strength 10.49; Genemark calls start at 50219 /note=SSC: 50219-50100 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein KIY76_gp89 [Mycobacterium phage Miramae] ],,NCBI, q1:s1 100.0% 7.55061E-19 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.241, -2.1695583717155773, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein KIY76_gp89 [Mycobacterium phage Miramae] ],,YP_010063168,100.0,7.55061E-19 SIF-HHPRED: SIF-Syn: