CDS complement (join(1. .384;68880. .68996)) /note=Phagesdb Function: top hit with cluster DE1 (5 counts) at 56% frequency, lysin a /note=Phagesdb BLAST: hits with Beagle and Pointis e-value significant, function unknown /note=HHPRED: top hit with GNAT FOLD, GCN5-N-ACETYLTRANSFERASE FOLD, e-value not significant /note=NCBI BLAST: top hits with Pontis and Beagle, e-value significant, hypothetical protein /note= /note=Start codon: original (384) because LORF already selected CDS 1065 - 1313 /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hits with Beagle and Pureglobe5 e-value significant, function unknown /note=HHPRED: top hit with Family of unknown function, e-value not significant, unknown function /note=NCBI BLAST: top hits with Beagle and Pointis, e-value significant, hypothetical protein /note= /note=Start codon: original (1065) CDS 1310 - 1540 /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hits with Beagle and Pointis, e-value significant, function unknown /note=HHPRED: top hit with Protein of unknown function, e-value not significant, unknown function /note=NCBI BLAST: top hits with Beagle and Odyssey 395, e-value significant, hypothetical protein /note= /note=Start codon: original (1310) CDS 1614 - 1709 /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hits with Pointis and Beagle, e-value significant, function unknown /note=HHPRED: top hit with Family of unknown function, e-value not significant, unknown function /note=NCBI BLAST: top hits with Pointis and Beagle, e-value significant, membrane protein /note=DeepTMHMM: one transmembrane domain confirmed by DeepTMHMM and SOSUI /note= /note=Start codon: edited to position 1614 to align with more AP2 phages (Beagle, Odyssey395, Pureglobe5) /note= - NB. original start codon (1554) aligned 1:1 with Pointis CDS 1709 - 1837 /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: hits with Beagle, Odyssey395, Pointis, and Pureglobe5, e-value significant, function unknown /note=HHPRED: top hit with Domain of unknown function, e-value not significant, unknown function /note=NCBI BLAST: top hits with Beagle, Pointis, as membrane protein and Pureglobe5, Odyssey395, as hypothetical protein, e-value significant. /note=DeepTMHMM: one transmembrane domain confirmed by DeepTMHMM and SOSUI /note=`double-check` /note= /note=Start codon: original (1709) CDS 1837 - 2073 /note=Phagesdb Function: top hit with subcluster AP2 (5 counts) at 63% frequency, dna binding protein /note=Phagesdb BLAST: hits with Beagle, Odyssey395, Pointis, and Pureglobe5, e-value significant, DNA binding protein /note=HHPRED: top hit with DNA polymerase eta, e-value not significant, DNA polymerase /note=NCBI BLAST: top hits with Beagle, Odyssey395 and Pointis as DNA binding protein, e-value significant. /note= /note=Start codon: original (1837) CDS 2070 - 2921 /note=Phagesdb Function: hit with subcluster A9 (1 count) at 100% frequency, rna polymerase sigma factor /note=Phagesdb BLAST: hits with Pointis, Odyssey395, Beagle, and Pureglobe5, e-value significant, function unknown /note=HHPRED: top hit with SMODS and SLOG-associating 2TM effector domain 1, e-value not significant /note=NCBI BLAST: top hits with Pointis, Odyssey395, Beagle, membrane protein, e-value significant. /note=DeepTMHMM: one transmembrane domain confirmed by DeepTMHMM and SOSUI; changed to membrane protein. /note= /note=Start codon: original (2070) CDS 2918 - 3463 /note=Phagesdb Function: hit with subcluster A1 (5 count) at 50% frequency, dna methylase /note=Phagesdb BLAST: hits with Odyssey395, Pointis, Pureglobe5, e-value significant, function unknown /note=HHPRED: top hit with Dodecameric ferritin homolog, e-value not significant /note=NCBI BLAST: top hits with Pointis and Odyssey395, hypothetical protein, e-value significant. /note=DeepTMHMM: no transmembrane domain confirmed by either DeepTMHMM or SOSUI /note= /note=Start codon: edited to position 2918 for 1:1 BLASTP alignment with Pointis, Beagle, Odyssey395, MellowYellow, and Pureglobe5. CDS 3460 - 3867 /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Beagle, e-value significant, function unknown /note=HHPRED: top hit with PGDYG protein, e-value significant /note=NCBI BLAST: top hits with Beagle, Pureglobe5, Pointis, Odyssey395, and MellowYellow hypothetical protein, e-value significant. /note= /note=Start codon: original (3460) CDS 3997 - 4125 /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Beagle, e-value significant, function unknown /note=HHPRED: top hit with Geopilin domain 1 protein, e-value not significant /note=NCBI BLAST: top hits with Beagle as hypothetical protein and MellowYellow as membrane protein, e-value significant. /note= /note=Start codon: original (3997) CDS 4125 - 4280 /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Beagle, e-value significant, function unknown /note=HHPRED: top hit with MobC-like protein, e-value not significant /note=NCBI BLAST: top hits with Beagle, Odyssey395, Pureglobe5, Pointis, and MellowYellow, hypothetical protein, e-value significant. /note= /note=Start codon: original (4125) CDS 4277 - 4525 /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Beagle, e-value significant, function unknown /note=HHPRED: top hit with Protein of unknown function, e-value not significant /note=NCBI BLAST: top hits with Beagle and MellowYellow, hypothetical protein, e-value significant. /note= /note=Start codon: original (4277) CDS 4522 - 4728 /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Odyssey395, e-value significant, function unknown /note=HHPRED: top hit with Secreted Virulence Immune System, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (4522) CDS 4725 - 4898 /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Odyssey395, e-value significant, function unknown /note=HHPRED: top hit with Cytochrome O ubiquinol oxidase, subunit II, e-value not significant /note=NCBI BLAST: top hits with Odyssey395 and Pureglobe5 as hypothetical protein, Pointis and Beagle as membrane protein hypothetical protein, e-value significant. /note=DeepTMHMM: one transmembrane domain confirmed by DeepTMHMM and SOSUI /note= /note=Start codon: original (4725) CDS 4895 - 5476 /note=Phagesdb Function: hits with subcluster AP2 (5 count) at 6% frequency, parb-like nuclease domain protein /note=Phagesdb BLAST: top hit with Beagle, e-value significant, ParB-like nuclease domain protein /note=HHPRED: top hit with ParB family protein; DNA-binding protein, e-value significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Beagle, ParB-like nuclease domain protein, e-value significant. /note=`double-check function name` /note= /note=Start codon: original (4895) CDS 5476 - 5715 /note=Phagesdb Function: hits with subcluster Q (21 count) at 36% frequency, lysin a /note=Phagesdb BLAST: top hit with Beagle, e-value significant, function unknown /note=HHPRED: top hit with Kinesin-like protein KIF2A-like, e-value not significant /note=NCBI BLAST: top hits with Beagle, hypothetical protein, e-value significant. /note=DeepTMHMM: no transmembrane domain confirmed by DeepTMHMM and SOSUI (soluble protein predicted) /note= /note=Start codon: edited to position 5476 for 1:1 BLASTP alignment with more AP2 phages, except Beagle CDS 5700 - 5798 /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Odyssey395, e-value significant, function unknown /note=HHPRED: top hit with Glycine rich protein family, e-value not significant /note=NCBI BLAST: top hits with Odyssey, Pointis, MellowYellow, and Pureglobe5 as a hypothetical protein, and Beagle as a membrane protein, e-value significant. /note= /note=Start codon: original (5700) CDS 5785 - 6303 /note=Phagesdb Function: hits with subcluster AP2 (3 count) at 4% frequency, helix-turn-helix dna binding domain /note=- Also hits with subcluster AP2 (1 count) at 1% frequency, dna binding protein; /note=- Also hits with subcluster AP2 (1 count) at 1% frequency, terminase, small subunit /note=Phagesdb BLAST: top hit with Odyssey395 and MellowYellow, e-value significant, helix-turn-helix DNA binding domain; /note=- Also hits with Pureglobe5, e-value significant, terminase small subunit; /note=- Also hits with Beagle, e-value significant, DNA binding protein /note=HHPRED: top hit with Protein of unknown function, e-value significant /note=NCBI BLAST: top hit with Odyssey, MellowYellow, Pointis as helix-turn-helix DNA binding domain protein, e-value significant. /note=- 2nd top hit with Beagle as DNA binding protein, e-value significant. /note=- Also top hit with Pureglobe5 as terminase small subunit, e-value significant /note=`double-check` /note= /note=Start codon: original (5785) CDS 6296 - 6715 /note=Phagesdb Function: hits with subcluster AP2 (3 count) at 6% frequency, ribbon-helix-helix dna binding domain protein /note=Phagesdb BLAST: hits with Pointis and Odyssey395, e-value significant, ribbon-helix-helix DNA binding domain protein /note=HHPRED: top hit with DNA-binding, ribbon-helix-helix, e-value significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Pointis, MellowYellow ribbon-helix-helix DNA binding domain protein, e-value significant. /note= /note=Start codon: original (6296) CDS 6728 - 7444 /note=Phagesdb Function: hits with subcluster AP2 (3 count) at 3% frequency, dna methyltransferase /note=Phagesdb BLAST: hits with Beagle, Odyssey395, Pureglobe5, e-value significant, DNA methyltransferase /note=HHPRED: top hit with DNA methyltransferase, e-value significant /note=NCBI BLAST: top hits with Beagle, Odyssey395, Pureglobe5, DNA methyltransferase, e-value significant. /note= /note=Start codon: original (6728) CDS 7441 - 9072 /note=Phagesdb Function: hits with subcluster AP2 (3 count) at 5% frequency, terminase /note=Phagesdb BLAST: hits with Beagle, Odyssey395, Pointis e-value significant, terminase /note=-NB. Also hits with Pureglobe5 and MellowYellow– terminase large subunit /note=HHPRED: top hit with Large subunit terminase, e-value significant /note=NCBI BLAST: top hits with Beagle, Odyssey395, Pointis, terminase, e-value significant. /note=-NB. also hits with MellowYellow and Pureglobe5, terminase, large subunit /note= /note=Start codon: original (7441) /note= /note=`double-check, revise if small subunit terminase is found` CDS 9130 - 9441 /note=Phagesdb Function: N/A, no entries /note=Phagesdb BLAST: top hit with MellowYellow, e-value significant, function unknown /note=HHPRED: top hit with Protein of unknown function, e-value significant /note=NCBI BLAST: top hits with Odyssey395, Pointis, MellowYellow, and Beagle as membrane protein, and Pureglobe5 as hypothetical protein, e-value significant. /note=DeepTMHMM: three transmembrane domains confirmed by DeepTMHMM and SOSUI /note=`double-check` /note= /note=Start codon: original (9130) CDS 9444 - 11330 /note=Phagesdb Function: hits with subcluster AP2 (5 count) at 8% frequency, portal protein /note=Phagesdb BLAST: top hit with MellowYellow, Odyssey395, Pureglobe5, and Beagle e-value significant, portal protein /note=HHPRED: top hit with Portal protein, e-value significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, MellowYellow, Pointis, Beagle, etc. as portal protein, e-value significant. /note= /note=Start codon: original (9444) CDS 11327 - 11470 /note=Phagesdb Function: N/A, no entries /note=Phagesdb BLAST: top hit with Beagle e-value significant, function unknown /note=HHPRED: top hit with Nsp1alpha N-terminal zinc finger, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Pointis, Beagle, hypothetical protein, e-value significant. /note= /note=Start codon: original (11327) CDS 11467 - 14130 /note=Phagesdb Function: hits with subcluster AP2 (4 count) at 9% frequency, capsid maturation protease /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, Pointis, MellowYellow e-value significant, capsid maturation protease /note=HHPRED: top hit with Portal protein, e-value significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Pointis, Beagle, MellowYellow capsid maturation protease, e-value significant. /note=- Beagle as capsid maturation protease and MuF-like fusion protein /note= /note=Start codon: original (11467) CDS 14427 - 16154 /note=Phagesdb Function: hits with subcluster AP2 (4 count) at 5% frequency, major capsid hexamer protein /note=Phagesdb BLAST: top hit with Pointis, Beagle, Pureglobe5, Odyssey395, e-value significant, major capsid hexamer protein /note=HHPRED: top hit with Endosome, Trafficking complex, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Pointis, Beagle, major capsid hexamer protein, e-value significant. /note= /note=Start codon: original (14427) CDS 16222 - 16980 /note=Phagesdb Function: hits with subcluster DC1 (4 count) at 100% frequency, major capsid pentamer protein /note=Phagesdb BLAST: top hit with Pointis, Beagle, Pureglobe5, Odyssey395, e-value significant, function unknown /note=HHPRED: top hit with major capsid protein, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Pointis, Beagle, hypothetical protein, e-value significant. /note= /note=Start codon: original (16222) CDS 16992 - 17441 /note=Phagesdb Function: hits with subcluster K4 (8 count) at 44% frequency, muf-like minor capsid protein /note=Phagesdb BLAST: top hit with Beagle, MellowYellow, Odyssey395, e-value significant, function unknown /note=HHPRED: top hit with Hypothetical protein SSo0622, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Pointis, Beagle, MellowYellow, hypothetical protein, e-value significant. /note= /note=Start codon: original (16992) CDS 17445 - 18365 /note=Phagesdb Function: hits with subcluster AP2 (5 count) at 7% frequency, major tail protein /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, MellowYellow, e-value significant, major tail protein /note=HHPRED: top hit with Tail Tube; Flagellotropic bacteriophage, Siphophage, Tail, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Pointis, Beagle, MellowYellow, major tail protein, e-value significant. /note= /note=Start codon: original (17445) CDS 18432 - 19370 /note=Phagesdb Function: hits with subcluster AP2 (2 count) at 3% frequency, endolysin; /note= - Also hits with subcluster AP2 (1 count) at 1% frequency, lysin a /note=Phagesdb BLAST: hits with Beagle as lysin a, e-value significant /note= - Also hits with MellowYellow and Pureglobe5 as endolysin, e-value significant /note=HHPRED: top hit with membrane protein, enzyme, e-value significant /note=NCBI BLAST: top hits with Beagle, lysin a, e-value significant. /note= - Also hits with Pureglobe5, MellowYellow, BruhMoment as endolysin, e-value significant /note=‘double-check (higher intergenomic similarity with Pureglobe5)’ /note= /note=Start codon: original (18432) CDS 19381 - 19998 /note=Phagesdb Function: hits with subcluster AP2 (5 count) at 7% frequency, head-to-tail adaptor /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, MellowYellow, e-value significant, head-to-tail adaptor /note=HHPRED: top hit with Head completion protein, e-value significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Pointis, Beagle, MellowYellow, head-to-tail adaptor, e-value significant. /note= /note=Start codon: original (19381) CDS 19998 - 20525 /note=Phagesdb Function: N/A, no entries /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, e-value significant, function unknown /note=HHPRED: top hit with KBTB, W-type LIR motif, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Pointis, Beagle, MellowYellow, hypothetical protein, e-value significant. /note= /note=Start codon: original (19998) CDS 20525 - 21055 /note=Phagesdb Function: hits with subcluster AP2 (1 count) at 4% frequency, minor tail protein /note=Phagesdb BLAST: top hit with Beagle, MellowYellow, Pointis, e-value significant, function unknown /note=- Also hits with Pureglobe5, e-value significant, minor tail protein /note=HHPRED: top hit with KBTB, Minor capsid protein, e-value significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Pointis, Beagle, MellowYellow, hypothetical protein, e-value significant. /note=`double-check` /note= /note=Start codon: original (20525) CDS 21119 - 21553 /note=Phagesdb Function: hits with subcluster AP2 (3 count) at 16% frequency, tail assembly chaperone /note=Phagesdb BLAST: top hit with Beagle and Odyssey395, e-value significant, function unknown /note= - Also hits with MellowYellow, Pointis, PureGlobe5, e-value significant, tail assembly chaperone /note=HHPRED: top hit with KBTB, Phage tail assembly chaperone, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Beagle, hypothetical protein, e-value significant. /note= - Also hits with Pureglobe5, Pointis, MellowYellow, tail assembly chaperone, e-value significant. /note=Slippery sequence: GGGAAAA /note= /note=Start codon: original (21119) CDS 21565 - 21693 /note=Phagesdb Function: hits with subcluster AP2 (3 count) at 33% frequency, tail assembly chaperone /note=Phagesdb BLAST: top hit with Beagle, Odyssey39, e-value significant, function unknown /note= - Also hits with Pureglobe5, Pointis, MellowYellow, e-value significant, tail assembly chaperone, /note=HHPRED: top hit with Mitochondrial ribosomal protein L55, e-value not significant /note=NCBI BLAST: hits with Beagle, Odyssey39, hypothetical protein, e-value significant /note= - Also hits with MellowYellow, Pureglobe5, Pointis, and Ranunculus as tail assembly chaperone e-value significant /note= /note=Slippery sequence: GGGAAAA /note= /note=Start codon: original (21565) CDS 21714 - 26597 /note=Phagesdb Function: hits with subcluster AP2 (5 count) at 6% frequency, tape measure protein /note=Phagesdb BLAST: top hit with Odyssey395, Beagle, Pointis, Pureglobe5, MellowYellow, e-value significant, tape measure protein /note=HHPRED: top hit with KBTB, Tape Measure Protein, e-value significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Pointis, Beagle, MellowYellow, tape measure protein, e-value significant. /note= /note=Start codon: original (21714) CDS 26607 - 27815 /note=Phagesdb Function: hits with subcluster AP2 (5 count) at 7% frequency, minor tail protein /note=Phagesdb BLAST: top hit with Odyssey395, Beagle, Pointis, Pureglobe5, MellowYellow, e-value significant, minor tail protein /note=HHPRED: top hit with Distal tail protein, Receptor-binding protein, Phage baseplate, genome injection device, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Pointis, Beagle, MellowYellow, minor tail protein, e-value significant. /note= /note=Start codon: original (26607) CDS 27820 - 28953 /note=Phagesdb Function: hits with subcluster AP2 (5 count) at 7% frequency, minor tail protein /note=Phagesdb BLAST: top hit with Odyssey395, Beagle, Pointis, Pureglobe5, e-value significant, minor tail protein /note=HHPRED: hits with Minor tail protein, e-value significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Pointis, Beagle, MellowYellow, minor tail protein, e-value significant. /note= /note=Start codon: original (27820) CDS 28946 - 29155 /note=Phagesdb Function: hits with subcluster DC1 (26 count) at 74% frequency, tape measure protein /note=Phagesdb BLAST: top hit with Odyssey395, Beagle, Pureglobe5, e-value significant, function unknown /note=HHPRED: hits with Thymidine kinase, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Beagle, MellowYellow, hypothetical protein, e-value significant. /note= /note=Start codon: original (28946) CDS 29186 - 30256 /note=Phagesdb Function: hits with subcluster AP2 (5 count) at 7% frequency, minor tail protein /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, MellowYellow, Beagle, Pointis, e-value significant, minor tail protein /note=HHPRED: hits with Major tropism determinant N-terminal domain, e-value significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Beagle, Pointis, MellowYellow, minor tail protein, e-value significant. /note= /note=Start codon: original (29186) CDS 30256 - 32040 /note=Phagesdb Function: hits with subcluster AP2 (5 count) at 9% frequency, minor tail protein /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, MellowYellow, Beagle, Pointis, e-value significant, minor tail protein /note=HHPRED: hits with Major tropism determinant N-terminal domain, e-value significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Beagle, Pointis, MellowYellow, minor tail protein, e-value significant. /note= /note=Start codon: original (30256) CDS 32068 - 34071 /note=Phagesdb Function: hits with subcluster AP2 (5 count) at 10% frequency, minor tail protein /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, MellowYellow, Beagle, Pointis, e-value significant, minor tail protein /note=HHPRED: hits with Signaling protein, e-value significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Beagle, Pointis, MellowYellow, minor tail protein, e-value significant. /note= /note=Start codon: original (32068) CDS 34405 - 34584 /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, MellowYellow, Beagle, Pointis, e-value significant, function unknown /note=HHPRED: hits with Domain of unknown function, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, Beagle, Pointis, MellowYellow, hypothetical protein, e-value significant. /note= /note=Start codon: original (34405) CDS 34589 - 34933 /note=Phagesdb Function: hits with subcluster BM (4 count) at 67% frequency, tape measure protein /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, MellowYellow, Beagle, Pointis, e-value significant, function unknown /note=HHPRED: hits with Protein of unknown function, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Beagle, Pointis, MellowYellow, as a membrane protein and Pureglobe5 as a hypothetical protein, e-value significant. /note=DeepTMHMM: one transmembrane domain confirmed by DeepTMHMM and SOSUI /note= /note=Start codon: original (34589) CDS 34930 - 35145 /note=Phagesdb Function: hits with subcluster CZ5 (1 count) at 50% frequency, tape measure protein /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, MellowYellow, Beagle, Pointis, e-value significant, function unknown /note=HHPRED: hits with Pre-mRNA-processing factor 19; Spliceosome, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Beagle, Pointis, MellowYellow, and Pureglobe5 as a hypothetical protein, e-value significant. /note= /note=Start codon: original (34930) CDS 35142 - 35525 /note=Phagesdb Function: hits with subcluster AO2 (12 count) at 71% frequency, holin /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, MellowYellow, Beagle, Pointis, e-value significant, function unknown /note=HHPRED: hits with Family of unknown function, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Beagle, Pointis, MellowYellow as a membrane protein and Pureglobe5 as a hypothetical protein, e-value significant. /note=DeepTMHMM: three transmembrane domains confirmed by DeepTMHMM and SOSUI /note= /note=Start codon: original (35142) CDS 35518 - 35811 /note=Phagesdb Function: hits with subcluster P4 (2 count) at 20% frequency, tape measure protein /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, MellowYellow, Beagle, Pointis, e-value significant, function unknown /note=HHPRED: hits with Family of unknown function, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Beagle, Pointis, MellowYellow as a membrane protein and Pureglobe5 as a hypothetical protein, e-value significant. /note=DeepTMHMM: two transmembrane domains confirmed by DeepTMHMM and SOSUI /note= /note=Start codon: original (35518) CDS 35821 - 37518 /note=Phagesdb Function: hits with subcluster AP2 (5 count) at 18% frequency, minor tail protein /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, Beagle, Pointis, e-value significant, minor tail protein /note= -Also hits with MellowYellow as function unknown, e-value significant /note=HHPRED: hits with Tail fiber protein; fiber, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Beagle, Pointis, and Pureglobe5 as a minor tail protein, e-value significant. /note= -Also hits with MellowYellow as hypothetical protein, e-value significant /note= /note=Start codon: original (35821) CDS complement (37656 - 38093) /note=Phagesdb Function: hits with subcluster AP2 (5 count) at 7% frequency, rusa-like resolvase /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, MellowYellow, AWGoat, Pointis, e-value significant, RusA-like resolvase /note=HHPRED: hits with Homologous recombination, DNA repair, resolvase, e-value significant /note=NCBI BLAST: top hits with Odyssey395, Pointis, MellowYellow, Puregloge5, and AWGoat as RusA-like resolvase, e-value significant. /note= /note=Start codon: original (38093) CDS complement (38083 - 38325) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, MellowYellow, Beagle, Pointis, e-value significant, function unknown /note=HHPRED: hits with PLP aminotransferase, mechanism-based inhibitor, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pointis, MellowYellow, Puregloge5, and Beagle as hypothetical protein, e-value significant. /note= /note=Start codon: original (38325) CDS complement (38322 - 38525) /note=Gene 51 /note=Phagesdb Function: hits with subcluster EH (2 count) at 50% frequency, major capsid protein /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, MellowYellow, Beagle, Pointis, e-value significant, function unknown /note=HHPRED: hits with Type IV pilus assembly protein, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pointis, MellowYellow, Puregloge5, and Beagle as hypothetical protein, e-value significant. /note= /note=Start codon: edited to position 38525 for 1:1 BLASTP alignment with MellowYellow, Odyssey395, Beagle, Pureglobe5, except Pointis CDS complement (38518 - 38811) /note=Phagesdb Function: hits with subcluster EH (3 count) at 20% frequency, helix-turn-helix dna binding domain /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, Beagle, Pointis, e-value significant, helix-turn-helix DNA binding domain protein /note=HHPRED: hits with DNA/RNA-binding 3-helical bundle, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pointis, Puregloge5, and Beagle as helix-turn-helix DNA binding domain protein, e-value significant. /note= /note=Start codon: original (38811) CDS complement (38808 - 39104) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, Beagle, Pointis, MellowYellow, e-value significant, function unknown /note=HHPRED: hits with antitoxin MqsA for MqsR toxin, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pointis, Puregloge5, MellowYellow and Beagle as hypothetical protein, e-value significant. /note= /note=Start codon: original (39104) CDS complement (39101 - 39523) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, Beagle, Pointis, MellowYellow, e-value significant, function unknown /note=HHPRED: hits with Domain of unknown function, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pointis, Puregloge5, MellowYellow and Beagle as hypothetical protein, e-value significant. /note= /note=Start codon: edited to position 39523 for 1:1 BLASTP alignment with Pointis, Odyssey395, Beagle, Pureglobe5 CDS complement (39520 - 39723) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, Beagle, Pointis, MellowYellow, e-value significant, function unknown /note=HHPRED: hits Uncharacterized protein conserved in bacteria, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pointis, Puregloge5, MellowYellow and Beagle as hypothetical protein, e-value significant. /note= /note=Start codon: original (39723) CDS complement (39720 - 39974) /note=Phagesdb Function: hits with subcluster AP2 (3 count) at 6% frequency, helix-turn-helix dna binding domain /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, Pointis, e-value significant, helix-turn-helix DNA binding domain protein /note= -Also hits with Beagle and MellowYellow as a DNA binding (domain) protein /note=HHPRED: hits Putative DNA-binding domain, e-value significant /note=NCBI BLAST: top hits with Odyssey395, Pointis, Puregloge5, helix-turn-helix DNA binding domain protein, e-value significant /note= -Also MellowYellow and Beagle, DNA binding (domain) protein, e-value significant. /note=`double-check, DNA binding or helix-turn-helix DNA binding domain protein` /note= /note=Start codon: original (39974) CDS complement (39971 - 40225) /note=Phagesdb Function: hits with subcluster AP2 (4 count) at 57% frequency, helix-turn-helix dna binding domain /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, Pointis, Beagle, and MellowYellow, e-value significant, helix-turn-helix DNA binding domain protein. /note=HHPRED: hits C-terminal domain of bacterial DnaA proteins, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pointis, Puregloge5, MellowYellow, and Beagle, helix-turn-helix DNA binding domain protein, e-value significant. /note= /note=Start codon: original (40225) CDS complement (40218 - 40406) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Pointis, Beagle, and MellowYellow, e-value significant, function unknown /note=HHPRED: hits with tRNA CCA-adding enzyme, e-value not significant /note=NCBI BLAST: top hits with Pointis, MellowYellow, and Beagle, hypothetical protein, e-value significant. /note= /note=Start codon: edited to position 40406 for 1:1 BLASTP alignment with Pointis, and Beagle, not MellowYellow CDS complement (40508 - 40897) /note=Phagesdb Function: hits with subcluster AP2 (42 count) at 95% frequency, dna primase/polymerase/helicase /note=Phagesdb BLAST: top hit with Ranunculus, BruhMoment, Pointis, Beagle, and MellowYellow, e-value significant, function unknown /note=HHPRED: hits with Hydrogenase/urease nickel incorporation, metallochaperone, e-value not significant /note=NCBI BLAST: top hits with Ranunculus, BruhMoment, Pointis, Beagle, and MellowYellow, hypothetical protein, e-value significant. /note= /note=Start codon: original (40897) CDS complement (40894 - 41082) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Pointis, Beagle, and MellowYellow, e-value significant, function unknown /note=HHPRED: hits with Modified RING-CH finger, H2 subclass, e-value not significant /note=NCBI BLAST: top hits with Pointis, Beagle, and MellowYellow, hypothetical protein, e-value significant.) /note= /note=Start Codon: original (41082) CDS complement (41079 - 41210) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Pointis, Beagle, and MellowYellow, e-value significant, function unknown /note=HHPRED: hits with Protein of unknown function, e-value not significant /note=NCBI BLAST: top hits with Pointis, Beagle, and MellowYellow, hypothetical protein, e-value significant. /note= /note=Start codon: original (41210) CDS complement (41210 - 41509) /note=Phagesdb Function: hits with subcluster A2 (8 count) at 33% frequency, helix-turn-helix dna binding domain /note=Phagesdb BLAST: top hit with Odyssey395, Pureglobe5, e-value significant, function unknown /note=HHPRED: hits with Conserved membrane protein, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Pureglobe5, hypothetical protein, e-value significant. /note= /note=Start codon: original (41509) CDS complement (41506 - 41829) /note=Phagesdb Function: hits with subcluster BH (3 count) at 75% frequency, tape measure protein /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, e-value significant, function unknown /note=HHPRED: hits with PetP, cytochrome b6f complex, SH3 domain, cyanobacteria, Thermosynechococcus elongatus, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, BillyTP, AWGoat hypothetical protein, e-value significant. /note= /note=Start codon: original (41829) CDS complement (41826 - 42206) /note=Phagesdb Function: hits with subcluster AE (2 count) at 50% frequency, dna helicase /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Ranunculus, Pointis, e-value significant, function unknown /note=HHPRED: hits with Domain of unknown function, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Ranunculus, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (42206) CDS complement (42203 - 42478) /note=Phagesdb Function: hits with subcluster R (9 count) at 90% frequency, muf-like minor capsid protein /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, e-value significant, function unknown /note=HHPRED: hits with histone fold domain, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (42478) CDS complement (42475 - 42783) /note=Phagesdb Function: hits with subcluster EA1 (29 count) at 88% frequency, mazg-like nucleotide pyrophosphohydrolase /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, e-value significant, function unknown /note=HHPRED: hits with PGDYG protein, e-value significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note=Start codon: original (42783) CDS complement (42783 - 43046) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, e-value significant, function unknown /note=HHPRED: hits with NURS complex subunit red1, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: edited to position 43046 for 1:1 BLASTP alignment with Pointis, Beagle, Pureglobe5, Odyssey395, not MellowYellow CDS complement (43046 - 43570) /note=Phagesdb Function: hits with subcluster CY (1 count) at 100% frequency, tape measure protein /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, e-value significant, function unknown /note=HHPRED: hits with Plasmid conjugation, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (43570) CDS complement (43603 - 43821) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, e-value significant, function unknown /note=HHPRED: hits with Sigma factor regulator C-terminal, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (43821) CDS complement (43818 - 44360) /note=Phagesdb Function: hits with subcluster M1 (6 count) at 33% frequency, endonuclease vii /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, e-value significant, function unknown /note=HHPRED: hits with 60S ribosomal export protein, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (44360) CDS complement (44357 - 44890) /note=Phagesdb Function: hits with subcluster A5 (1 count) at 50% frequency, tape measure protein /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, e-value significant, function unknown /note=HHPRED: hits with Family of unknown function, e-value significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (44890) CDS complement (44965 - 45237) /note=Phagesdb Function: hits with subcluster DJ (5 count) at 56% frequency, major tail protein /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, e-value significant, function unknown /note=HHPRED: hits with Polycomb protein Scm, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (45237) CDS complement (45230 - 45463) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, e-value significant, function unknown /note=HHPRED: hits with Bacterial PH domain, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (45463) CDS complement (45465 - 45776) /note=Phagesdb Function: hits with subcluster AP1 (1 count) at 25% frequency, exonuclease /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, e-value significant, function unknown /note= - NB Hits with Wilde, e-value significant, exonuclease /note=HHPRED: hits with Transposon Tn7 transposition protein TnsD, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= - NB Also hits with Wilde, e-value significant, exonuclease /note=`double-check` /note= /note=Start codon: original (45776) CDS complement (45773 - 46018) /note=Phagesdb Function: hits with subcluster DQ (3 count) at 38% frequency, tape measure protein /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pureglobe5, Pointis, e-value significant, function unknown /note=HHPRED: hits with Pili subunits, e-value not significant /note=NCBI BLAST: top hits with Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (46018) CDS complement (46015 - 46344) /note=Phagesdb Function: hits with subcluster J (2 count) at 25% frequency, tape measure protein /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pureglobe5, MellowYellow, Pointis, e-value significant, function unknown /note=HHPRED: hits with Volume-regulated anion channel subunit LRRC8D, e-value not significant /note=NCBI BLAST: top hits with Beagle, Odyssey395, Pureglobe5, Pointis, MellowYellow hypothetical protein, e-value significant. /note= /note=Start codon: original (46344) CDS complement (46337 - 47509) /note=Phagesdb Function: hits with subcluster AP2 (2 count) at 3% frequency, cas4 exonuclease /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, e-value significant, function unknown /note= - NB. also hits with MellowYellow, Pointis e-value significant, Cas4 exonuclease /note= - NB. also hits with Pureglobe5, e-value significant, RecB-like exonuclease/helicase /note=HHPRED: hits with CRISPR/Cas system-associated protein Cas4. CRISPR, e-value significant /note=NCBI BLAST: top hits with Beagle, Odyssey395, hypothetical protein, e-value significant. /note= - NB. also hits with MellowYellow, Pointis e-value significant, Cas4 exonuclease /note= - NB. also hits with Pureglobe5, e-value significant, RecB-like exonuclease/helicase /note=`double-check... note gap` /note= /note=Start codon: original (47509) CDS complement (47713 - 48600) /note=Phagesdb Function: hits with subcluster AP2 (3 count) at 5% frequency, dna binding protein /note=Phagesdb BLAST: top hit with Beagle, MellowYellow, Pointis, e-value significant, DNA binding protein /note= - NB. also hits with Pureglobe5, Odyssey395, e-value significant, RecA-like DNA recombinase /note=HPRED: hits with DNA repair and recombination protein RAD52, e-value significant /note=NCBI BLAST: top hits with Beagle, Pointis, MellowYellow, Ranunculus, DNA binding protein, e-value significant. /note= - NB. also hits with Pureglobe5, Odyssey395, Rizwana e-value significant, RecA-like DNA recombinase /note=`double-check` /note= /note=Start codon: original (48600) CDS complement (48753 - 48965) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, e-value significant, function unknown /note=HHPRED: hits with Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (48965) CDS complement (48965 - 49438) /note=Phagesdb Function: hits with subcluster AP2 (4 count) at 5% frequency, deoxyuridine triphosphatase /note=Phagesdb BLAST: top hit with Beagle, Pointis, MellowYellow, Pureglobe5, Odyssey395, e-value significant, dUTPase /note=HPRED: hits with Deoxyuridine 5`-triphosphate nucleotidohydrolase, e-value significant /note=NCBI BLAST: top hits with Beagle, Pointis, MellowYellow, Pureglobe5, Odyssey395, dUTPase, e-value significant. /note= /note=Start codon: edited to position 49438 for 1:1 BLASTP alignment with Pointis, Beagle, Ranunculus, Odyssey395 CDS complement (49435 - 49641) /note=Phagesdb Function: hits with subcluster BF (1 count) at 100% frequency, scaffolding protein /note=Phagesdb BLAST: top hit with Beagle, Pointis, MellowYellow, Pureglobe5, Odyssey395, e-value significant,function unknown /note=HPRED: hits with Clostridial binary toxin B/anthrax toxin PA domain 3, e-value not significant /note=NCBI BLAST: top hits with Beagle, Pointis, MellowYellow, Pureglobe5, Odyssey395, hypothetical protein, e-value significant. /note= /note=Start codon: original (49641) CDS complement (49641 - 49967) /note=Phagesdb Function: hits with subcluster AP2 (5 count) at 6% frequency, hnh endonuclease /note=Phagesdb BLAST: top hit with Pointis, MellowYellow, Pureglobe5, Odyssey395, e-value significant, HNH endonuclease /note=HPRED: hits with Type IV secretion system, e-value not significant /note=NCBI BLAST: top hits with Pointis, MellowYellow, Pureglobe5, Odyssey395, HNH endonuclease, e-value significant. /note= /note=Start codon: original (49967) CDS complement (49964 - 50161) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Pointis, Beagle, Pureglobe5, Odyssey395, e-value significant, function unknown /note=HPRED: hits with Type IV secretion system, e-value not significant /note=NCBI BLAST: top hits with Pointis, MellowYellow, Pureglobe5, Odyssey395, HNH endonuclease, e-value significant. /note=`double-check` /note= /note=Start codon: edited to position 50161 for 1:1 BLASTP alignment with Pointis, Beagle, Pureglobe5, Odyssey395 CDS complement (50158 - 50511) /note=Phagesdb Function: hits with subcluster A2 (17 count) at 71% frequency, scaffolding protein /note=Phagesdb BLAST: top hit with Pointis, Beagle, Pureglobe5, Odyssey395, e-value significant, function unknown /note=HPRED: hits with C-terminal homology domain, e-value not significant /note=NCBI BLAST: top hits with Pointis, MellowYellow, Pureglobe5, Odyssey395, hypothetical protein, e-value significant. /note= /note=Start codon: original (50511) CDS complement (50508 - 50843) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Pointis, Beagle, Pureglobe5, Odyssey395, e-value significant, function unknown /note=HPRED: hits with Coiled-coil domain containing 32, e-value not significant /note=NCBI BLAST: top hits with Pointis, MellowYellow, Pureglobe5, hypothetical protein, e-value significant. /note= /note=Start codon: original (50843) CDS complement (50840 - 51304) /note=Phagesdb Function: hits with subcluster AP2 (4 count) at 11% frequency, DNA binding protein /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Pureglobe5, Odyssey395, e-value significant, DNA binding protein /note= - NB hits with Pointis, e-value significant, helix-turn-helix DNA binding domain protein /note=HPRED: hits with PUTATIVE TRANSCRIPTIONAL REGULATOR, e-value significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Ranunculus, DNA binding protein, e-value significant. /note= - NB hits with Pointis, e-value significant, helix-turn-helix DNA binding domain protein /note= /note=Start codon: original (51304) CDS complement (51301 - 51867) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Pureglobe5, Odyssey395, Pointis, e-value significant, function unknown /note=HPRED: hits with Coronin-1A; coiled coil, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (51867) CDS complement (51864 - 52250) /note=Phagesdb Function: hits with subcluster AL (1 count) at 100% frequency, DNA binding protein /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Pureglobe5, Odyssey395, Pointis, e-value significant, function unknown /note=HPRED: hits with Domain of unknown function, e-value significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: edited to position 52250 for 1:1 BLASTP alignment with Pointis, Beagle, Pureglobe5, Odyssey395 CDS complement (52247 - 52765) /note=binding domain /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Pureglobe5, Odyssey395, Pointis, e-value significant, function unknown /note=HPRED: hits with Protein of unknown function, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (52765) CDS complement (52806 - 53246) /note=Phagesdb Function: hits with subcluster DC1 (27 count) at 90% frequency, minor tail protein /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Pureglobe5, Odyssey395, Pointis, e-value significant, function unknown /note=HPRED: hits with Family of unknown function, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (53246) CDS complement (53262 - 54866) /note=Phagesdb Function: hits with subcluster AP2 (3 count) at 4% frequency, dna primase/helicase /note=Phagesdb BLAST: top hit with, Pureglobe5, MellowYellow, Pointis, e-value significant, DNA primase/helicase /note= - NB. hits with Beagle, e-value significant, DNA primase/polymerase/helicase /note= - NB. hits with Odyssey395, e-value significant, DNA primase /note=HPRED: hits with DNA primase/helicase; helicase,, e-value significant /note=NCBI BLAST: hits with Pureglobe5, MellowYellow, Pointis, DNA primase/helicase, e-value significant. /note= - NB. hits with Beagle, e-value significant, DNA primase/polymerase/helicase /note= - NB. hits with Odyssey395, e-value significant, DNA primase /note=`double-check` /note= /note=Start codon: original (54866) CDS complement (54908 - 55651) /note=Phagesdb Function: hits with subcluster AP2 (3 count) at 8% frequency, helix-turn-helix dna binding domain /note=Phagesdb BLAST: hits with MellowYellow, Pureglobe5, Pointis, e-value significant, helix-turn-helix DNA binding domain protein /note= - NB. Also hits with Odyssey395, e-value significant, RepA-like replication initiator /note= - NB. also hits with Beagle, e-value significant, DNA binding protein /note=HPRED: hits with DNA replication protein DnaD, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Pureglobe5, Pointis, helix-turn-helix DNA binding domain protein, e-value significant. /note= - NB. Also hits with Odyssey395, RepA-like replication initiator, e-value significant /note= - NB. also hits with Beagle, DNA binding protein, e-value significant /note=`double-check` /note= /note=Start codon: original (55651) CDS complement (55920 - 56420) /note=Phagesdb Function: hits with subcluster CS2 (31 count) at 91% frequency, tape measure protein /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Pureglobe5, Odyssey395, Pointis, e-value significant, function unknown /note=HPRED: hits with De novo designed protein, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: edited to position 56420 for 1:1 BLASTP alignment with Pointis, Beagle, Pureglobe5, Odyssey395, MellowYellow CDS complement (56489 - 57094) /note=Phagesdb Function: hits with subcluster AP2 (2 count) at 2% frequency, ssdna binding protein /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Pureglobe5, Odyssey395, Pointis, e-value significant, SSB protein /note=HPRED: hits with Single-stranded DNA-binding protein 2, e-value significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, SSB protein, e-value significant. /note=Start codon: original (57094) CDS complement (57098 - 57400) /note=Phagesdb Function: hits with subcluster K5 (17 count) at 37% frequency, terminase /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Pureglobe5, Odyssey395, Pointis, e-value significant, function unknown /note=HPRED: hits with L,D-transpeptidase C-terminal domain, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (57400) CDS complement (57545 - 58036) /note=Phagesdb Function: hits with subcluster F1 (50 count) at 79% frequency, portal protein /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Pureglobe5, Odyssey395, Pointis, e-value significant, function unknown /note=HPRED: hits with Phosphoribosylformylglycinamidine synthase II, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: edited to position 58036 for 1:1 BLASTP alignment with Pointis, Beagle, Pureglobe5, Odyssey395 CDS complement (58040 - 59365) /note=Phagesdb Function: hits with subcluster AW (5 count) at 19% frequency, portal protein /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Pureglobe5, Odyssey395, Pointis, e-value significant, function unknown /note=HPRED: hits with De novo designed protein LFR1, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (59365) CDS complement (59377 - 60288) /note=Phagesdb Function: hits with subcluster A2 (2 count) at 100% frequency, minor tail protein /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Pureglobe5, Odyssey395, Pointis, e-value significant, function unknown /note=HPRED: hits with Cytochrome b-c1 complex subunit Rieske, mitochondrial, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (60288) CDS complement (60325 - 60570) /note=Phagesdb Function: hits with subcluster EG (1 count) at 100% frequency, capsid maturation protease /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Pureglobe5, Odyssey395, Pointis, e-value significant, function unknown /note=HPRED: hits with Fe-S metabolism associated domain, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pureglobe5, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (60570) CDS complement (60563 - 60763) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pointis, e-value significant, function unknown /note=HPRED: hits with Myb-like DNA-binding domain, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pointis, hypothetical protein, e-value significant. /note= /note=Start codon: original (60763) CDS complement (60747 - 61397) /note=Phagesdb Function: hits with subcluster G1 (10 count) at 91% frequency, lysin a /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pointis, Pureglobe5, e-value significant, function unknown /note=HPRED: hits with SWI/SNF complex subunit SMARCC1, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pointis, Pureglobe5, hypothetical protein, e-value significant. /note= /note=Start codon: original (61397) CDS complement (61518 - 61775) /note=Phagesdb Function: hits with subcluster AK (3 count) at 60% frequency, helix-turn-helix dna binding domain /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pointis, Pureglobe5, e-value significant, function unknown /note=HPRED: hits with Putative bacteriophage terminase small subunit, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pointis, Pureglobe5, hypothetical protein, e-value significant. /note= /note=Start codon: original (61775) CDS complement (61775 - 62446) /note=Phagesdb Function: hits with subcluster AK (3 count) at 20% frequency, tail assembly chaperone /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pointis, Pureglobe5, e-value significant, function unknown /note=HPRED: hits with Immune Mapped Protein 2 (IMP2) N-terminal domain, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pointis, Pureglobe5, hypothetical protein, e-value significant. /note= /note=Start codon: original (62446) CDS complement (62513 - 62899) /note=Phagesdb Function: hits with subcluster K1 (4 count) at 50% frequency, helix-turn-helix dna binding domain /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pointis, Pureglobe5, e-value significant, function unknown /note=HPRED: hits with Domain of Unknown Function, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pointis, Pureglobe5, hypothetical protein, e-value significant. /note= /note=Start codon: original (62899) CDS complement (62896 - 63729) /note=Phagesdb Function: hits with subcluster A2 (1 count) at 50% frequency, capsid maturation protease /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pointis, Pureglobe5, e-value significant, function unknown /note=HPRED: hits with Uncharacterised protein family, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pointis, Pureglobe5, hypothetical protein, e-value significant. /note= /note=Start codon: original (63729) CDS 63732 - 64043 /note=Phagesdb Function: hits with subcluster B1 (5 count) at 56% frequency, tape measure protein /note=Phagesdb BLAST: top hit with Pureglobe5, e-value significant, function unknown /note=HPRED: hits with High affinity immunoglobulin epsilon receptor subunit gamma, e-value not significant /note=NCBI BLAST: top hits with Pureglobe5, hypothetical protein, e-value significant. /note= /note=Start codon: original (63732) CDS complement (63920 - 64048) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pointis, Pureglobe5, e-value significant, function unknown /note=HPRED: hits with Domain of unknown function, e-value not significant /note=NCBI BLAST: top hits with MellowYellow, Beagle, Odyssey395, Pointis, Pureglobe5, hypothetical protein, e-value significant. /note= /note=Start codon: original (64048) CDS complement (64045 - 64158) /note=Phagesdb Function: hits with subcluster BI1 (521count) at 100% frequency, tape measure protein /note=Phagesdb BLAST: top hit with MellowYellow, Beagle, Odyssey395, Pointis, Pureglobe5, e-value significant, function unknown /note=HPRED: hits with YtxH-like protein, e-value not significant /note=NCBI BLAST: hits with Beagle, Pointis, Pureglobe5, hypothetical protein, e-value significant. /note= - NB. Also hits with Odyssey395, MellowYellow, membrane protein, e-value significant /note=`double-check` /note=Start codon: original (64158) CDS complement (64155 - 64256) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, Pureglobe5, e-value significant, function unknown /note=HPRED: hits with ECA polysaccharide chain length modulation protein, e-value not significant /note=NCBI BLAST: top hits with Pointis, Pureglobe5, hypothetical protein, e-value significant. /note= - NB. Also hits with Odyssey395, Beagle, membrane protein, e-value significant /note=DeepTMHMM: one transmembrane domain confirmed by DeepTMHMM and SOSUI /note=`double-check` /note= /note=Start codon: original (64256) CDS complement (64256 - 64516) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, Pureglobe5, MellowYellow e-value significant, function unknown /note=HPRED: hits with Phenylacetate-CoA oxygenase subunit PaaB, e-value not significant /note=NCBI BLAST: top hits with Odyssey395, Beagle, Pointis, Pureglobe5, MellowYellow, hypothetical protein, e-value significant. /note= /note=Start codon: original (64516) CDS complement (64553 - 64672) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, Pureglobe5, Ranunculus e-value significant, function unknown /note=HPRED: hits with Beta-dystroglycan; complex membrane stability signaling, e-value not significant /note=NCBI BLAST: top hits with Beagle, Odyssey395, Pointis, Pureglobe5, Ranunculus, hypothetical protein, e-value significant. /note= /note=Start codon: original (64672) CDS complement (64669 - 64920) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, Pureglobe5, MellowYellow e-value significant, function unknown /note=HPRED: hits with Anti-cleavage anti-greA transcription factor gfh1, e-value not significant /note=NCBI BLAST: top hits with Beagle, Odyssey395, Pureglobe5, MellowYellow, Ranunculus, hypothetical protein, e-value significant. /note= - NB. Also hits with Pointis, membrane protein, e-value significant /note=Start codon: original (64920) CDS complement (64966 - 65136) /note=Phagesdb Function: hits with subcluster DJ (9 count) at 100% frequency, lysin a /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, Pureglobe5, MellowYellow e-value significant, function unknown /note=HPRED: hits with FERM, e-value not significant /note=NCBI BLAST: top hits with Beagle, Odyssey395, Pureglobe5, Pointis, MellowYellow, Ranunculus, hypothetical protein, e-value significant. /note= /note=Start codon: original (65136) CDS complement (65133 - 65426) /note=Phagesdb Function: hits with subcluster AU3 (2 count) at 40% frequency, tape measure protein /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, Pureglobe5, MellowYellow e-value significant, function unknown /note=HPRED: hits with maltose-binding protein MalE3, e-value not significant /note=NCBI BLAST: top hits with Beagle, Odyssey395, Pureglobe5, Pointis, MellowYellow, Ranunculus, hypothetical protein, e-value significant. /note= /note=Start codon: original (65426) CDS complement (65505 - 65732) /note=Phagesdb Function: hits with subcluster K1 (1 count) at 50% frequency, muf-like minor capsid protein /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, Pureglobe5, MellowYellow e-value significant, function unknown /note=HPRED: hits with TPR1-like,, e-value not significant /note=NCBI BLAST: top hits with Beagle, Odyssey395, Pointis, Ranunculus, hypothetical protein, e-value significant. /note=Start codon: original (65732) CDS complement (65880 - 66353) /note=Phagesdb Function: hits with subcluster B4 (1 count) at 50% frequency, major capsid hexamer protein /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, Pureglobe5, MellowYellow e-value significant, function unknown /note=HPRED: hits with Symbiodinium, Photosystem I, e-value not significant /note=NCBI BLAST: top hits with Beagle, Odyssey395, Pointis, Ranunculus, hypothetical protein, e-value significant. /note=Start codon: original (66353) CDS complement (66350 - 66538) /note=Phagesdb Function: hits with subcluster BE1 (10 count) at 100% frequency, dna primase/polymerase /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, Pureglobe5, MellowYellow e-value significant, function unknown /note=HPRED: hits with FYVE-related domain found in Rab effector Noc2 and similar proteins, e-value not significant /note=NCBI BLAST: top hits with Beagle, Pointis, MellowYellow, Pureglobe5, hypothetical protein, e-value significant. /note=Start codon: original (66538) CDS complement (66692 - 66871) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, e-value significant, function unknown /note=HPRED: hits with zinc-finger, e-value not significant /note=NCBI BLAST: top hits with Beagle, Pointis, Odyssey395, Pureglobe5, hypothetical protein, e-value significant. /note=Start codon: original (66871) CDS complement (66904 - 67119) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, MellowYellow, Pureglobe5, e-value significant, function unknown /note=HPRED: hits with PROTEIN (CATECHOL OXIDASE), e-value not significant /note=NCBI BLAST: top hits with Beagle, Pointis, Odyssey395, Pureglobe5, MellowYellow, hypothetical protein, e-value significant. /note= /note=Start codon: edited to position 67119 for 1:1 BLASTP alignment with Pointis, Beagle, Pureglobe5, Odyssey395 CDS complement (67119 - 67397) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, Pureglobe5, e-value significant, function unknown /note=HPRED: hits with histidine utilization protein HutD and related proteins, e-value not significant /note=NCBI BLAST: top hits with Beagle, Pointis, Odyssey395, Pureglobe5, hypothetical protein, e-value significant. /note= /note=Start codon: original (67397) CDS complement (67454 - 67726) /note=Phagesdb Function: N/A, 0 entries /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, Pureglobe5, e-value significant, function unknown /note=HPRED: hits with Complex I, NADH dehydrogenase, Mitochondrion proton pumping, Ubiquinone, Oxidoreductase, e-value not significant /note=NCBI BLAST: top hits with Beagle, Pointis, Odyssey395, Pureglobe5, hypothetical protein, e-value significant. /note= /note=Start codon: edited to position 67726 for 1:1 BLASTP alignment with Pointis, Beagle, MellowYellow CDS complement (68019 - 68792) /note=Phagesdb Function: hits with subcluster CZ1 (10 count) at 29% frequency, minor tail protein /note=Phagesdb BLAST: top hit with Beagle, Odyssey395, Pointis, Pureglobe5, e-value significant, function unknown /note=HPRED: hits with C-type lectin domain family 12 member A, e-value not significant /note=NCBI BLAST: top hits with Beagle, Pointis, Odyssey395, Pureglobe5, hypothetical protein, e-value significant. /note= /note=Start codon: original (68792) Genes added in gaps • SEA_KUBULIX_1 (706-1068): Hypothetical protein (BLAST hits with AP phages) • SEA_KUBULIX_26 (14133-14276): Hypothetical protein (BLAST hits with AP phages) • SEA_KUBULIX_122 (67737-67943) REVERSE: Hypothetical protein (BLAST hits with AP phages) Deleted phams in PECAAN • Gene 1: The first gene in PECAAN is reversed from 384 to 68880. This gene does not appear to be annotated in other cluster AP phages. This gene was deleted in the DNA Master minimal file. • Gene 106: Deleted in the DNA Master minimal file. Overlaps with other reverse genes. AP phages appear to mostly have genes encoded on the top strand through the first 36-38k nucleotides and reverse genes on the right side of the genome.