CDS 105 - 413
  /gene="1"
  /product="gp1"
  /function="terminase, small subunit"
  /locus tag="StuartMinion_1"
    /note=Original Glimmer call @bp 105 has strength 9.13; Genemark calls start at 105
    /note=SSC: 105-413 CP: yes SCS: both ST: SS BLAST-Start: [terminase small subunit [Arthrobacter phage Brynnie] ],,NCBI, q1:s1 100.0% 8.86674E-59 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.893, -2.935178901640172, yes F: terminase, small subunit SIF-BLAST: ,,[terminase small subunit [Arthrobacter phage Brynnie] ],,QOP64900,97.0588,8.86674E-59 SIF-HHPRED: Terminase_4 ; Phage terminase, small subunit,,,PF05119.17,75.4902,95.3 SIF-Syn: According to Phamerator, it shows that there is synteny with the other genes such as PhluffyCoco & AmiCi24.
    /note=According to phagesDB BLAST, this program predicts terminase, small subunit as a function in other phages, with PhluffyCoco showing a score of 205. In addition, this function has a high probability score of 95.3% in HHPRED.

CDS 373 - 1995
  /gene="2"
  /product="gp2"
  /function="terminase, large subunit"
  /locus tag="StuartMinion_2"
    /note=Original Glimmer call @bp 373 has strength 14.81; Genemark calls start at 373
    /note=SSC: 373-1995 CP: yes SCS: both ST: SS BLAST-Start: [terminase large subunit [Arthrobacter phage Renna12]],,NCBI, q1:s1 100.0% 0.0 GAP: -41 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.11, -7.042289884676138, no F: terminase, large subunit SIF-BLAST: ,,[terminase large subunit [Arthrobacter phage Renna12]],,UXE05354,98.1481,0.0 SIF-HHPRED: Terminase large subunit; genome packaging, bacteriophage, ATPase, nuclease, VIRAL PROTEIN; HET: BR; 2.2A {Enterobacteria phage HK97},,,6Z6D_A,91.4815,100.0 SIF-Syn: Stuartminion gene 2 showcases synteny within the AS3 subcluster alongside AmiCi24, Atlantica, Babushka, Camara, DanHam62, Glotell, HamCheese, Juno112, Khumphrey, Leona, PhluffyCoCo, Rattail, and RedFox.
    /note=Evidence given by HHpred and PhagesDb supports the function of this gene being a Terminase large subunit.

CDS 2011 - 2325
  /gene="3"
  /product="gp3"
  /function="RNA binding protein"
  /locus tag="StuartMinion_3"
    /note=Original Glimmer call @bp 2011 has strength 11.96; Genemark calls start at 2005
    /note=SSC: 2011-2325 CP: yes SCS: both-gl ST: SS BLAST-Start: [RNA binding protein [Arthrobacter phage Andrew] ],,NCBI, q1:s9 100.0% 2.87413E-45 GAP: 15 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.648, -3.4470622626190464, yes F: RNA binding protein SIF-BLAST: ,,[RNA binding protein [Arthrobacter phage Andrew] ],,YP_009815690,82.5688,2.87413E-45 SIF-HHPRED: Small nuclear ribonucleoprotein F; U1 snRNP, Spliceosome, Pre-mRNA splicing, Ribonucleoprotein, splicing; HET: MG, EPE, GTP; 3.3A {Homo sapiens},,,4PJO_F,71.1538,99.3 SIF-Syn: 

CDS 2342 - 4264
  /gene="4"
  /product="gp4"
  /function="portal protein"
  /locus tag="StuartMinion_4"
    /note=Original Glimmer call @bp 2342 has strength 15.07; Genemark calls start at 2342
    /note=SSC: 2342-4264 CP: yes SCS: both ST: SS BLAST-Start: [portal protein [Arthrobacter phage Leona]],,NCBI, q1:s1 100.0% 0.0 GAP: 16 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.728, -3.791591111008034, yes F: portal protein SIF-BLAST: ,,[portal protein [Arthrobacter phage Leona]],,QRI45413,97.0266,0.0 SIF-HHPRED: Portal protein; Archaeal virus, portal, portal capsid interface, Mg ions, VIRUS; HET: MG, HIP; 2.342A {Haloferax tailed virus 1},,,8QQN_PL,95.7812,100.0 SIF-Syn: 

CDS 4289 - 5038
  /gene="5"
  /product="gp5"
  /function="Capsid maturation protease"
  /locus tag="StuartMinion_5"
    /note=Original Glimmer call @bp 4289 has strength 12.16; Genemark calls start at 4289
    /note=SSC: 4289-5038 CP: yes SCS: both ST: SS BLAST-Start: [capsid maturation protease [Arthrobacter phage RedFox] ],,NCBI, q1:s1 100.0% 1.5902E-146 GAP: 24 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.648, -3.3060637489568596, no F: Capsid maturation protease SIF-BLAST: ,,[capsid maturation protease [Arthrobacter phage RedFox] ],,WNM72792,95.6,1.5902E-146 SIF-HHPRED: Peptidase_S78 ; Caudovirus prohead serine protease,,,PF04586.22,61.4458,99.9 SIF-Syn: StuartMinion gene 5 show synteny with other genes like RedFox, Rattail, PhluffyCoco, Leona, KHumphrey, Juno112, HamCheese, Glotell, DanHam62, Camara, Babushka, Atlantica, and AmiCi24.
    /note=Capsid maturation protease is the only predicted function for this gene seen when using PhagesDB BLAST, and the HHPRED data shows that it has an e-value of 2.3e-19 and a probability of 99.9, which supports capsid maturation protease being the function of this gene.

CDS 5120 - 6427
  /gene="6"
  /product="gp6"
  /function="Major capsid protein"
  /locus tag="StuartMinion_6"
    /note=Original Glimmer call @bp 5120 has strength 16.49; Genemark calls start at 5120
    /note=SSC: 5120-6427 CP: yes SCS: both ST: SS BLAST-Start: [major capsid protein [Arthrobacter phage Leona]],,NCBI, q1:s12 100.0% 0.0 GAP: 81 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.164, -4.376977241479107, no F: Major capsid protein SIF-BLAST: ,,[major capsid protein [Arthrobacter phage Leona]],,QRI45415,95.5157,0.0 SIF-HHPRED: Scaffolding domain delta; Prohead I, icosahedral symmetry, HK97, phage, capsid, VIRUS; 3.5A {Escherichia phage HK97},,,8FQK_A,99.5402,100.0 SIF-Syn: StuartMinion gene 6 show synteny with other genes like RedFox, Rattail, PhluffyCoco, Leona, KHumphrey, Juno112, HamCheese, Glotell, DanHam62, Camara, Babushka, Atlantica, and AmiCi24.
    /note=Major capsid protein was the only predicted function for this gene seen when using PhagesDB BLAST. HHPRED data also gives an e-score of 2.8e-34, coverage of 99.5402, and probability of 100. These pieces of evidence support major capsid protein being the function of this gene.

CDS 6509 - 6718
  /gene="7"
  /product="gp7"
  /function="hypothetical protein"
  /locus tag="StuartMinion_7"
    /note=Original Glimmer call @bp 6509 has strength 18.4; Genemark calls start at 6509
    /note=SSC: 6509-6718 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein HOU53_gp08 [Arthrobacter phage Andrew] ],,NCBI, q1:s1 100.0% 4.17247E-21 GAP: 81 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.062, -2.583959800616441, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein HOU53_gp08 [Arthrobacter phage Andrew] ],,YP_009815694,89.6552,4.17247E-21 SIF-HHPRED: conserved hypothetical protein; T1445, NYSGXRC, UNKNOWN ORF, COG3027, PSI, Protein Structure Initiative, New York SGX Research Center for Structural; HET: MSE; 2.5A {Pseudomonas aeruginosa PAO1} SCOP: d.244.1.1,,,1T3U_A,31.8841,79.0 SIF-Syn: According to Phamerator, it shows that there is synteny with the other genes such as PhluffyCoco & AmiCi24.
    /note=The function of the gene in the subcluster AS3 of phage StuartMinion remains elusive. First, PhagesDB Function Frequency only provides a function for subcluster AS1, not AS3. Furthermore, Phages BLAST predicts the function as "unknown," while both HHPRED and NCBI BLAST predominantly display hypothetical functions. Given the lack of conclusive evidence and the inconsistent information provided by these four programs, it is not possible to determine a specific function for gene 7 in StuartMinion`s AS3 subcluster. Consequently, the function will be classified as "unknown" until further data becomes available.

CDS 6740 - 7327
  /gene="8"
  /product="gp8"
  /function="head-to-tail adaptor"
  /locus tag="StuartMinion_8"
    /note=Original Glimmer call @bp 6740 has strength 9.88; Genemark calls start at 6740
    /note=SSC: 6740-7327 CP: yes SCS: both ST: SS BLAST-Start: [head-to-tail adaptor [Arthrobacter phage Leona]],,NCBI, q1:s1 100.0% 3.19689E-123 GAP: 21 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.648, -3.368377069717189, yes F: head-to-tail adaptor SIF-BLAST: ,,[head-to-tail adaptor [Arthrobacter phage Leona]],,QRI45417,95.3846,3.19689E-123 SIF-HHPRED: Head-to-tail adaptor; Bacteriophage, portal, VIRAL PROTEIN;{Mycobacterium phage Bxb1},,,9D94_Ga,91.7949,97.6 SIF-Syn: StuartMinion Gene 8 exhibits synteny within the AS3 subcluster, alongside RedFox, Rattail, PhluffyCoco, Leona, KHumphrey, Juno112, HamCheese, Glotell, DanHam62, Camara, Babushka, Atlantica, and AmiCi24.
    /note=Evidence by HHPred, NCBI BLAST, and PhagesDB supports the function of this gene to be a head-to-tail adaptor.

CDS 7338 - 7676
  /gene="9"
  /product="gp9"
  /function="head-to-tail stopper"
  /locus tag="StuartMinion_9"
    /note=Original Glimmer call @bp 7338 has strength 9.85; Genemark calls start at 7338
    /note=SSC: 7338-7676 CP: yes SCS: both ST: SS BLAST-Start: [head-to-tail stopper [Arthrobacter phage Leona]],,NCBI, q1:s1 100.0% 8.58878E-70 GAP: 10 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.991, -4.73920793635854, no F: head-to-tail stopper SIF-BLAST: ,,[head-to-tail stopper [Arthrobacter phage Leona]],,QRI45418,95.5357,8.58878E-70 SIF-HHPRED: Head-to-tail stopper; Bacteriophage, portal, VIRAL PROTEIN;{Mycobacterium phage Bxb1},,,9D94_Hc,91.0714,99.7 SIF-Syn: StuartMinion Gene 9 exhibits synteny within the AS3 subcluster, alongside RedFox, Rattail, PhluffyCoco, Leona, KHumphrey, Juno112, HamCheese, Glotell, DanHam62, Camara, Babushka, Atlantica, and AmiCi24.
    /note=Evidence shown by HHPred, NCBI BLAST, and PhagesDB supports the function of this gene to be a head-to-tail stopper.

CDS 7681 - 7935
  /gene="10"
  /product="gp10"
  /function="hypothetical protein"
  /locus tag="StuartMinion_10"
    /note=Original Glimmer call @bp 7681 has strength 10.15; Genemark calls start at 7681
    /note=SSC: 7681-7935 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_ORCANUS_11 [Arthrobacter phage Orcanus]],,NCBI, q1:s1 100.0% 2.25349E-38 GAP: 4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.556, -5.725362593517833, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ORCANUS_11 [Arthrobacter phage Orcanus]],,QOI67656,91.6667,2.25349E-38 SIF-HHPRED: DUF5403 ; Family of unknown function (DUF5403),,,PF17395.7,77.381,95.0 SIF-Syn: 
    /note=GeneMark graph shows that it captures all of the gene coding potential
    /note=This is a gene because there are still pieces of evidence that is is a gene such as GeneMark showing coding potential being captured
    /note=Starterator: Not much evidence on starterator to come to a conclusion so a lot of the evidence comes from PECAAN

CDS 7928 - 8371
  /gene="11"
  /product="gp11"
  /function="tail terminator"
  /locus tag="StuartMinion_11"
    /note=Original Glimmer call @bp 7928 has strength 9.24; Genemark calls start at 7916
    /note=SSC: 7928-8371 CP: yes SCS: both-gl ST: SS BLAST-Start: [tail terminator [Arthrobacter phage RedFox] ],,NCBI, q1:s1 98.6395% 6.08645E-92 GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.556, -5.5843640798556455, no F: tail terminator SIF-BLAST: ,,[tail terminator [Arthrobacter phage RedFox] ],,WNM72798,97.2603,6.08645E-92 SIF-HHPRED: Tail terminator; Bacteriophage, portal, VIRAL PROTEIN;{Mycobacterium phage Bxb1},,,9D94_Ic,95.9184,99.8 SIF-Syn: 
    /note=The best option according to the national database shows for the function, tail terminator and the following NCBI blast results show for tail terminator so the abundance in that result shows how gene 11 is most likely a tail terminator gene.

CDS 8406 - 8987
  /gene="12"
  /product="gp12"
  /function="major tail protein"
  /locus tag="StuartMinion_12"
    /note=Original Glimmer call @bp 8406 has strength 17.82; Genemark calls start at 8406
    /note=SSC: 8406-8987 CP: yes SCS: both ST: SS BLAST-Start: [major tail protein [Arthrobacter phage Leona] ],,NCBI, q1:s1 100.0% 6.80402E-126 GAP: 34 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.648, -3.368377069717189, yes F: major tail protein SIF-BLAST: ,,[major tail protein [Arthrobacter phage Leona] ],,QRI45421,94.8186,6.80402E-126 SIF-HHPRED: Major tail protein; Bacteriophage, tail tube, VIRUS, VIRAL PROTEIN;{Mycobacterium phage Bxb1},,,9D9L_J,97.9275,99.9 SIF-Syn: 100% functional synteny with other members of the pham.
    /note=excellent e-value and probability scores from HHPRED as well as synteny with other members of the pham led to this functional call.

CDS 9092 - 9445
  /gene="13"
  /product="gp13"
  /function="tail assembly chaperone"
  /locus tag="StuartMinion_13"
    /note=Original Glimmer call @bp 9092 has strength 13.36; Genemark calls start at 9092
    /note=SSC: 9092-9445 CP: yes SCS: both ST: SS BLAST-Start: [tail assembly chaperone [Arthrobacter phage Cupello]],,NCBI, q1:s1 100.0% 1.90784E-62 GAP: 104 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.745, -5.189725184110477, yes F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Arthrobacter phage Cupello]],,WPM94339,92.3077,1.90784E-62 SIF-HHPRED: GP24_25 ; Mycobacteriophage tail assembly protein,,,PF17388.7,87.1795,99.1 SIF-Syn: Synteny with other AS3 phages.
    /note=not a gene on its own. this is part of a sequence containing all of genes 13/14/15. glimmer and genemark misinterpreted a frameshift and read it as 3 separate genes, but based on starterator data this sequence likely goes from 9092bp-9442bp for a total length of 350 bp.

CDS join(9092..9418,9418..9816)
  /gene="14"
  /product="gp14"
  /function="tail assembly chaperone"
  /locus tag="StuartMinion_14"
    /note=
    /note=SSC: 9092-9816 CP: yes SCS: neither ST: SS BLAST-Start: [tail assembly chaperone [Arthrobacter phage Leona] ],,NCBI, q1:s1 100.0% 2.57858E-144 GAP: -354 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.745, -5.189725184110477, yes F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Arthrobacter phage Leona] ],,QRI45423,92.5311,2.57858E-144 SIF-HHPRED: GP24_25 ; Mycobacteriophage tail assembly protein,,,PF17388.7,41.4938,98.5 SIF-Syn: Synteny w/ other AS3 phages
    /note=-1 frame shift CDS join (9092-9418, 9418-9816)

CDS 9820 - 12108
  /gene="15"
  /product="gp15"
  /function="tape measure protein"
  /locus tag="StuartMinion_15"
    /note=Original Glimmer call @bp 9820 has strength 8.84; Genemark calls start at 9871
    /note=SSC: 9820-12108 CP: yes SCS: both-gl ST: SS BLAST-Start: [tape measure protein [Arthrobacter phage Leona]],,NCBI, q1:s1 99.7375% 0.0 GAP: 2 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.164, -4.455662434380964, no F: tape measure protein SIF-BLAST: ,,[tape measure protein [Arthrobacter phage Leona]],,QRI45424,93.4383,0.0 SIF-HHPRED: Tape Measure Protein, gp57; phage tail, tail tip, tape measure protein, VIRAL PROTEIN; 3.7A {Staphylococcus virus 80alpha},,,6V8I_BF,11.1549,99.9 SIF-Syn: According to Starterator, there is high syteny of not only the same basepair start site, but also high probability of matching a tape measure protein sequence in the HHpred database.
    /note=All members of this pham share this function, HHPRED maintains the prediction that this is a Tape Measure protein with an e value of 3.3e-15 and a probability of 99.9. NCBI BLAST matches this top hit naming all observable similar genes of other genomes as a tape measure protein.

CDS 12108 - 12959
  /gene="16"
  /product="gp16"
  /function="minor tail protein"
  /locus tag="StuartMinion_16"
    /note=Original Glimmer call @bp 12108 has strength 12.59; Genemark calls start at 12108
    /note=SSC: 12108-12959 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Arthrobacter phage Andrew] ],,NCBI, q1:s1 100.0% 7.90782E-165 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.049, -4.6353190821692705, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage Andrew] ],,YP_009815703,91.1661,7.90782E-165 SIF-HHPRED: Baseplate Centerpiece, gp25; Myophage, redox trigger, disulfides, VIRUS; 3.2A {Agrobacterium phage Milano},,,8FQC_e1,47.3498,86.4 SIF-Syn: On the PECAAN platform, when scrolled below at the phagesdb function frequency, there are 7 genes in the same pham and same AS3 subcluster which have been recognized to have the function of a minor tail protein.
    /note=As suggested in the bioinformatics guide, paying attention to the e-value on the Phagesdb BLAST, the value being 1e-159 is considered relatively good because it means there is a high likelihood of matching the other sequences with that function.

CDS 12969 - 14216
  /gene="17"
  /product="gp17"
  /function="minor tail protein"
  /locus tag="StuartMinion_17"
    /note=Original Glimmer call @bp 12969 has strength 10.66; Genemark calls start at 12969
    /note=SSC: 12969-14216 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Arthrobacter phage RedFox] ],,NCBI, q1:s1 99.759% 0.0 GAP: 9 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.991, -4.6768946155982105, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage RedFox] ],,WNM72804,96.1353,0.0 SIF-HHPRED: Prophage MuSo2, 43 kDa tail protein; MuSo2, Shewanella oneidensis MR-1, Structural Genomics, PSI-2, Protein Structure Initiative, Midwest Center for Structural Genomics, MCSG; HET: MSE; 2.1A {Shewanella oneidensis} SCOP: b.106.1.1,,,3CDD_A,91.3253,99.9 SIF-Syn: Has syntenty with most phages in AS3 subcluster also in other clusters. For example, PhluffyCoco, Leona, KHumphrey, etc
    /note=Data from Phagesdb, NCBI BLAST supports minor tail protein as the function. HHPRED although omits the word "minor" supports the function as a tail protein.

CDS 14221 - 15183
  /gene="18"
  /product="gp18"
  /function="minor tail protein"
  /locus tag="StuartMinion_18"
    /note=Original Glimmer call @bp 14221 has strength 7.71; Genemark calls start at 14221
    /note=SSC: 14221-15183 CP: no SCS: both ST: SS BLAST-Start: [minor tail protein [Arthrobacter phage Leona]],,NCBI, q4:s5 99.0625% 9.83383E-160 GAP: 4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.382, -4.512227065926137, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage Leona]],,QRI45427,93.7695,9.83383E-160 SIF-HHPRED: DUF3540 ; Protein of unknown function (DUF3540),,,PF12059.13,31.875,91.5 SIF-Syn: Syntenty with phages in the AS3 cluster like Leona, Juno112, Renna12, etc.
    /note=Data from Phagesdb, NCBI BLAST supports function as minor tail protein. However, HHPRED supports baseplate assembly protein V, with low coverage and e-value but high probability percent.

CDS 15193 - 15816
  /gene="19"
  /product="gp19"
  /function="minor tail protein"
  /locus tag="StuartMinion_19"
    /note=Original Glimmer call @bp 15193 has strength 10.16; Genemark calls start at 15223
    /note=SSC: 15193-15816 CP: yes SCS: both-gl ST: SS BLAST-Start: [minor tail protein [Arthrobacter phage RedFox] ],,NCBI, q1:s1 98.5507% 2.1962E-115 GAP: 9 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.836, -5.080292895251628, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage RedFox] ],,WNM72806,85.9903,2.1962E-115 SIF-HHPRED: Gp15 protein; Listeria, homotrimeric, receptor binding protein, Bacteriophage, VIRAL PROTEIN; HET: 1PE, ACT; 1.7A {Listeria phage PSA},,,6R5W_B,57.4879,94.8 SIF-Syn: yes, phagesdb function frequency and phagesdb blast show synteny.
    /note=PhagesDB function frequency and phagesdb blast show this function. additionally NCBI blast shows good values for this function.

CDS 15817 - 16239
  /gene="20"
  /product="gp20"
  /function="membrane protein"
  /locus tag="StuartMinion_20"
    /note=Original Glimmer call @bp 15817 has strength 6.21; Genemark calls start at 15817
    /note=SSC: 15817-16239 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_EESA_21 [Arthrobacter phage Eesa]],,NCBI, q1:s1 100.0% 7.42924E-60 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.92, -4.825272756372522, no F: membrane protein SIF-BLAST: ,,[hypothetical protein SEA_EESA_21 [Arthrobacter phage Eesa]],,WAB09131,83.5714,7.42924E-60 SIF-HHPRED: DUF3325 ; Protein of unknown function (DUF3325),,,PF11804.13,65.0,63.2 SIF-Syn: no, which also supports no known function.
    /note=this is supported by lack of data in the given programs.

CDS 16249 - 16545
  /gene="21"
  /product="gp21"
  /function="hypothetical protein"
  /locus tag="StuartMinion_21"
    /note=Original Glimmer call @bp 16249 has strength 14.08; Genemark calls start at 16249
    /note=SSC: 16249-16545 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_LEONA_21 [Arthrobacter phage Leona]],,NCBI, q1:s1 100.0% 3.1771E-55 GAP: 9 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.394, -3.8366550365551095, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_LEONA_21 [Arthrobacter phage Leona]],,QRI45430,96.9388,3.1771E-55 SIF-HHPRED: ZapG-like ; Z-ring associated protein G-like,,,PF06295.17,71.4286,97.0 SIF-Syn: This gene shows synteny with Renna 12, Glotell, and Andrew in the AS3 subcluster.
    /note=The function was determined using data from NCBI Blast, HHpred, and PhagesDB.

CDS 16606 - 17655
  /gene="22"
  /product="gp22"
  /function="endolysin"
  /locus tag="StuartMinion_22"
    /note=Original Glimmer call @bp 16606 has strength 15.89; Genemark calls start at 16606
    /note=SSC: 16606-17655 CP: yes SCS: both ST: SS BLAST-Start: [endolysin [Arthrobacter phage Renna12]],,NCBI, q1:s1 100.0% 0.0 GAP: 60 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.739, -3.1165893723772498, no F: endolysin SIF-BLAST: ,,[endolysin [Arthrobacter phage Renna12]],,UXE05374,89.5184,0.0 SIF-HHPRED: Endolysin; amidase-2 domain, HYDROLASE; HET: ZN; 2.27A {Staphylococcus phage GH15},,,4OLS_A,57.8797,99.2 SIF-Syn: StuartMinion-22 has synteny with PhluffyCoco
    /note=This function is well supported by the data given in the following programs: Phagesdb FF, Phagesdb BLAST, HHPRED, and NCBI BLAST

CDS 17655 - 17969
  /gene="23"
  /product="gp23"
  /function="holin"
  /locus tag="StuartMinion_23"
    /note=Original Glimmer call @bp 17655 has strength 10.74; Genemark calls start at 17655
    /note=SSC: 17655-17969 CP: yes SCS: both ST: SS BLAST-Start: [holin [Arthrobacter phage RedFox]],,NCBI, q1:s1 97.1154% 1.46018E-55 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.046, -4.702334767722353, no F: holin SIF-BLAST: ,,[holin [Arthrobacter phage RedFox]],,WNM72810,90.6542,1.46018E-55 SIF-HHPRED: Phage_r1t_holin ; Putative lactococcus lactis phage r1t holin,,,PF16945.10,56.7308,70.7 SIF-Syn: StuartMinion-24 has synteny with RedFox
    /note=This function is well supported by Phagesdb FF and Phagesdb BLAST

CDS complement (18103 - 18429)
  /gene="24"
  /product="gp24"
  /function="helix-turn-helix DNA binding domain"
  /locus tag="StuartMinion_24"
    /note=Original Glimmer call @bp 18294 has strength 5.52; Genemark calls start at 18294
    /note=SSC: 18429-18103 CP: yes SCS: both-cs ST: NI BLAST-Start: [DNA binding protein [Arthrobacter phage Galaxy] ],,NCBI, q46:s1 58.3333% 8.25087E-33 GAP: -139 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.62, -3.3633765724101092, yes F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[DNA binding protein [Arthrobacter phage Galaxy] ],,YP_009594372,95.2381,8.25087E-33 SIF-HHPRED: Recombination Directionality Factor RdfS; Excisionase, Recombination Directionality Factor, winged helix-turn-helix, superhelix, DNA BINDING PROTEIN; HET: GOL; 2.45A {Mesorhizobium japonicum R7A},,,8DGL_B,51.8519,98.8 SIF-Syn: General synteny with RedFox gene_26
    /note=PhagesDB information doesn’t correlate with what is shown on Phamerator and therefore the information provided may not be accurate. PhagesDB FF and PhagesDB BLAST show no evidence. However, HHPred and NCBI-BLAST supports function as a DNA Binding Protein.

CDS complement (18291 - 18503)
  /gene="25"
  /product="gp25"
  /function="hypothetical protein"
  /locus tag="StuartMinion_25"
    /note=Genemark calls start at 18503
    /note=SSC: 18503-18291 CP: yes SCS: genemark ST: SS BLAST-Start: [hypothetical protein SEA_LEONA_26 [Arthrobacter phage Leona]],,NCBI, q1:s1 98.5714% 7.46847E-33 GAP: 2 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.915, -5.885737104557237, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_LEONA_26 [Arthrobacter phage Leona]],,QRI45434,90.0,7.46847E-33 SIF-HHPRED: DUF1854 ; Domain of unknown function (DUF1854),,,PF08909.16,34.2857,32.0 SIF-Syn: 
    /note=Gene 26 on phamerator
    /note=Most evidence from phagesdb BLAST

CDS complement (18506 - 18898)
  /gene="26"
  /product="gp26"
  /function="hypothetical protein"
  /locus tag="StuartMinion_26"
    /note=Original Glimmer call @bp 18898 has strength 2.74; Genemark calls start at 18898
    /note=SSC: 18898-18506 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_JAMUN_51 [Arthrobacter phage Jamun]],,NCBI, q16:s41 87.6923% 4.52939E-14 GAP: 78 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.149, -4.99877661786583, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_JAMUN_51 [Arthrobacter phage Jamun]],,UXE05197,40.6897,4.52939E-14 SIF-HHPRED: DUF6384 ; Family of unknown function (DUF6384),,,PF19911.4,86.9231,88.0 SIF-Syn: NA
    /note=Evidence mostly Phagesdb BLAST, not enough evidence for any one function

CDS complement (18977 - 19696)
  /gene="27"
  /product="gp27"
  /function="hypothetical protein"
  /locus tag="StuartMinion_27"
    /note=Original Glimmer call @bp 19696 has strength 7.7; Genemark calls start at 19696
    /note=SSC: 19696-18977 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein HOU53_gp30 [Arthrobacter phage Andrew] ],,NCBI, q1:s1 99.1632% 1.44726E-105 GAP: 106 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.986, -3.5088110057482766, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein HOU53_gp30 [Arthrobacter phage Andrew] ],,YP_009815716,76.0331,1.44726E-105 SIF-HHPRED: fvmX5 ; FtsH ternary system domain X5,,,PF20003.4,26.3598,82.3 SIF-Syn: PhluffyCoco and AmiCi24- no function determined
    /note=-All hit functions (I.e lysm-like endolysin, lipoprotein, minor tail protein, tape measure protein, endolysin) are from genes in different, separate phases from different Subclusters than StuartMinion
    /note=-Other phages in the same subcluster (AS3) does not call for a protein as well.

CDS complement (19803 - 20996)
  /gene="28"
  /product="gp28"
  /function="tyrosine integrase"
  /locus tag="StuartMinion_28"
    /note=Original Glimmer call @bp 20996 has strength 7.03; Genemark calls start at 20996
    /note=SSC: 20996-19803 CP: yes SCS: both ST: SS BLAST-Start: [tyrosine integrase [Arthrobacter phage Renna12]],,NCBI, q1:s1 100.0% 0.0 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.818, -5.03807843799195, no F: tyrosine integrase SIF-BLAST: ,,[tyrosine integrase [Arthrobacter phage Renna12]],,UXE05386,97.4811,0.0 SIF-HHPRED: Integrase; PROTEIN-DNA COMPLEX, DNA BINDING PROTEIN-DNA COMPLEX; HET: PTR; 3.8A {Enterobacteria phage lambda} SCOP: d.163.1.1, d.10.1.4,,,1Z1B_A,94.7103,100.0 SIF-Syn: 

CDS complement (20989 - 21354)
  /gene="29"
  /product="gp29"
  /function="immunity repressor"
  /locus tag="StuartMinion_29"
    /note=Genemark calls start at 21354
    /note=SSC: 21354-20989 CP: yes SCS: genemark ST: SS BLAST-Start: [immunity repressor [Arthrobacter phage Leona]],,NCBI, q1:s1 98.3471% 5.87745E-69 GAP: 205 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.199, -2.8084998623841946, yes F: immunity repressor SIF-BLAST: ,,[immunity repressor [Arthrobacter phage Leona]],,QRI45444,89.6,5.87745E-69 SIF-HHPRED: Stl; Mobile Genetic Element, Repressor, SaPI, HTH domain, DNA BINDING PROTEIN; HET: MSE; 2.901A {Staphylococcus aureus},,,7P4A_A,96.6942,98.2 SIF-Syn: 
    /note=PhagesDB Function Frequency
    /note=--> Immunity Repressor was chosen due to the fact that StuartMinion’s Pham number (3796) was also chosen from other phages in the same subcluster (AS3)
    /note=
    /note=PhagesDB BLAST
    /note=--> PhluffyCoco shows the function “Immunity Repressor” with a score of 207 and an E-Value of 8e-54
    /note=
    /note=HHPred
    /note=--> 7P4A_A shows a description of Repressor with a coverage of 97%, probability of 98.2%, and E-Value of 0.00058 (the lowest and closest to 0)
    /note=
    /note=NCBI-BLAST
    /note=--> YP_009815794 shows the low E-Value at 1.59789e-57 and has a coverage of 99% and has high identity/alignment at >76.2% at 2025-03-10T05:27:21
    /note=adamson2 changed the coding capacity to yes at 2025-03-10T05:27:21
    /note=adamson2 changed synteny to PhagesDB Function Frequency
    /note=--> Recommends both functions: Immunity Repressor (Frequency (%): 7)
    /note=
    /note=PhagesDB BLAST
    /note=--> Other phages that have high synteny in the same subcluster (AS3) have called for Immunity Repressor with the same E-Value
    /note=
    /note=NCBI-BLAST
    /note=--> Similar results from the previous programs–Repressor

CDS 21560 - 21829
  /gene="30"
  /product="gp30"
  /function="helix-turn-helix DNA binding domain"
  /locus tag="StuartMinion_30"
    /note=Original Glimmer call @bp 21560 has strength 13.67; Genemark calls start at 21560
    /note=SSC: 21560-21829 CP: yes SCS: both ST: SS BLAST-Start: [helix-turn-helix DNA binding domain protein [Arthrobacter phage Leona] ],,NCBI, q1:s1 100.0% 2.09701E-50 GAP: 205 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.404, -3.893834241316366, no F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding domain protein [Arthrobacter phage Leona] ],,QRI45445,96.6292,2.09701E-50 SIF-HHPRED: a.35.1.2 (C:) P22 C2 repressor, DNA-binding domain {Salmonella bacteriophage P22 [TaxId: 10754]} | CLASS: All alpha proteins, FOLD: lambda repressor-like DNA-binding domains, SUPFAM: lambda repressor-like DNA-binding domains, FAM: Phage repressors,,,SCOP_d3jxbc_,68.5393,98.7 SIF-Syn: In the Phagesdb BLAST, Renna12, Andrew, KHumphrey, RedFox, Juno112,  PhluffyCoco, and Leona all have synteny. NCBI BLAST also gives us a good idea of this.
    /note=phages db function frequency and  NCBI BLAST support this gene to have the helix-turn-helix DNA binding protein function.

CDS 21830 - 22069
  /gene="31"
  /product="gp31"
  /function="excise"
  /locus tag="StuartMinion_31"
    /note=Original Glimmer call @bp 21830 has strength 10.15; Genemark calls start at 21830
    /note=SSC: 21830-22069 CP: yes SCS: both ST: SS BLAST-Start: [helix-turn-helix DNA binding domain protein [Arthrobacter phage Renna12]],,NCBI, q1:s1 98.7342% 1.19646E-40 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.362, -4.25339475005661, no F: excise SIF-BLAST: ,,[helix-turn-helix DNA binding domain protein [Arthrobacter phage Renna12]],,UXE05389,87.8049,1.19646E-40 SIF-HHPRED: Recombination Directionality Factor RdfS; Excisionase, Recombination Directionality Factor, winged helix-turn-helix, superhelix, DNA BINDING PROTEIN; HET: GOL; 2.45A {Mesorhizobium japonicum R7A},,,8DGL_C,70.8861,98.8 SIF-Syn: In the Phagesdb BLAST, Renna12, Andrew, KHumphrey, RedFox, Juno112,  PhluffyCoco, and Leona all have synteny. NCBI BLAST also gives us a good idea of this.
    /note=phages db function frequency and NCBI BLAST support this gene to have the helix-turn-helix DNA binding protein function.

CDS 22066 - 23079
  /gene="32"
  /product="gp32"
  /function="RecE-like exonuclease"
  /locus tag="StuartMinion_32"
    /note=Original Glimmer call @bp 22066 has strength 14.39; Genemark calls start at 22081
    /note=SSC: 22066-23079 CP: yes SCS: both-gl ST: SS BLAST-Start: [RecE-like exonuclease [Arthrobacter phage RedFox] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.141, -2.356391881054909, no F: RecE-like exonuclease SIF-BLAST: ,,[RecE-like exonuclease [Arthrobacter phage RedFox] ],,WNM72827,97.3294,0.0 SIF-HHPRED: Uncharacterized protein R354; MIMIVIRE, Cas4-like, nuclease, R354, NUCLEAR PROTEIN; 2.806A {Acanthamoeba polyphaga mimivirus},,,5YET_B,91.6914,99.9 SIF-Syn: Synteny with other AS pahges

CDS 23080 - 23877
  /gene="33"
  /product="gp33"
  /function="RecT-like DNA pairing protein"
  /locus tag="StuartMinion_33"
    /note=Original Glimmer call @bp 23080 has strength 16.58; Genemark calls start at 23080
    /note=SSC: 23080-23877 CP: yes SCS: both ST: SS BLAST-Start: [RecT-like DNA pairing protein [Arthrobacter phage KHumphrey]],,NCBI, q1:s1 100.0% 0.0 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.141, -2.2763497933341483, yes F: RecT-like DNA pairing protein SIF-BLAST: ,,[RecT-like DNA pairing protein [Arthrobacter phage KHumphrey]],,XIJ70206,98.1132,0.0 SIF-HHPRED: RecT; DNA Recombination, DNA Annealing, DNA BINDING PROTEIN; 4.5A {Listeria innocua Clip11262},,,7UBB_E,93.2076,100.0 SIF-Syn: 

CDS 23874 - 24473
  /gene="34"
  /product="gp34"
  /function="DNA methyltransferase"
  /locus tag="StuartMinion_34"
    /note=Original Glimmer call @bp 23874 has strength 4.21; Genemark calls start at 23874
    /note=SSC: 23874-24473 CP: yes SCS: both ST: SS BLAST-Start: [DNA methyltransferase [Arthrobacter phage Renna12]],,NCBI, q1:s1 100.0% 6.06652E-121 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.611, -6.376677624715306, no F: DNA methyltransferase SIF-BLAST: ,,[DNA methyltransferase [Arthrobacter phage Renna12]],,UXE05392,92.5743,6.06652E-121 SIF-HHPRED: DNA (cytosine-5-)-methyltransferase; DNA methyltransferase, TRANSFERASE; HET: SAH;{Neurospora crassa},,,9BAP_A,94.9749,99.9 SIF-Syn: Has high syntony however displaced from the two closest phages.
    /note=Agreed upon heavily by all sources.

CDS 24526 - 25020
  /gene="35"
  /product="gp35"
  /function="DNA methyltransferase"
  /locus tag="StuartMinion_35"
    /note=Original Glimmer call @bp 24430 has strength 9.03; Genemark calls start at 24625
    /note=SSC: 24526-25020 CP: yes SCS: both-cs ST: NI BLAST-Start: [DNA methyltransferase [Arthrobacter phage RedFox] ],,NCBI, q3:s46 98.7805% 1.14198E-81 GAP: 52 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.078, -5.595837784824238, no F: DNA methyltransferase SIF-BLAST: ,,[DNA methyltransferase [Arthrobacter phage RedFox] ],,WNM72830,65.534,1.14198E-81 SIF-HHPRED: Cyt_C5_DNA_methylase; Cytosine-C5 specific DNA methylases; Methyl transfer reactions play an important role in many aspects of biology.,,,cd00315,33.5366,97.1 SIF-Syn: High synteny (100% of pham is called for this function)
    /note=All fully annotated members of this pham have been called as DNA methylase or methyltransferase
    /note=Although HHPRED shows coverage as only 43.5115%, the probability shown is 96.6%.

CDS 25038 - 25397
  /gene="36"
  /product="gp36"
  /function="helix-turn-helix DNA binding domain"
  /locus tag="StuartMinion_36"
    /note=Original Glimmer call @bp 25038 has strength 10.23; Genemark calls start at 25017
    /note=SSC: 25038-25397 CP: yes SCS: both-gl ST: NI BLAST-Start: [helix-turn-helix DNA binding domain protein [Arthrobacter phage KHumphrey]],,NCBI, q1:s1 100.0% 1.21962E-69 GAP: 17 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.648, -3.3060637489568596, yes F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding domain protein [Arthrobacter phage KHumphrey]],,XIJ70209,92.437,1.21962E-69 SIF-HHPRED: Conserved protein; Transcription regulation, winged helix turn helix motif, Antibiotic resistance, DNA BINDING PROTEIN; 3.15A {Mycobacterium tuberculosis H37Rv},,,8JXK_A,75.6302,98.9 SIF-Syn: Synteny with other AS phages

CDS 25394 - 25864
  /gene="37"
  /product="gp37"
  /function="RusA-like resolvase"
  /locus tag="StuartMinion_37"
    /note=Original Glimmer call @bp 25394 has strength 11.43; Genemark calls start at 25394
    /note=SSC: 25394-25864 CP: yes SCS: both ST: SS BLAST-Start: [RusA-like resolvase [Arthrobacter phage Leona]],,NCBI, q1:s1 100.0% 2.46355E-99 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.354, -4.000014702296222, yes F: RusA-like resolvase SIF-BLAST: ,,[RusA-like resolvase [Arthrobacter phage Leona]],,QRI45452,96.7949,2.46355E-99 SIF-HHPRED: d.79.6.1 (A:) automated matches {Escherichia coli [TaxId: 562]} | CLASS: Alpha and beta proteins (a+b), FOLD: Bacillus chorismate mutase-like, SUPFAM: Holliday junction resolvase RusA, FAM: Holliday junction resolvase RusA,,,SCOP_d2h8ea_,96.1538,99.8 SIF-Syn: Synteny with other AS3 phages
    /note=The gene under consideration does not exhibit synteny with other genes (e.g., PhluffyCoco or AmiCi24), which limits the confidence in its functional assignment. Synteny is one of the key factors that contribute to determining a gene`s function, alongside sequence homology. However, multiple bioinformatics tools, including PhagesDB Function Frequency, PhagesDB BLAST, HHPRED, and NCBI BLAST, consistently predict that this gene encodes a RusA-like resolvase. This convergent evidence from multiple computational analyses provides substantial support for the proposed functional annotation of this gene.

CDS 25861 - 26658
  /gene="38"
  /product="gp38"
  /function="helix-turn-helix DNA binding domain"
  /locus tag="StuartMinion_38"
    /note=Original Glimmer call @bp 25861 has strength 10.38; Genemark calls start at 25876
    /note=SSC: 25861-26658 CP: yes SCS: both-gl ST: SS BLAST-Start: [helix-turn-helix DNA binding domain protein [Arthrobacter phage Renna12]],,NCBI, q1:s1 94.717% 1.08722E-128 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.493, -3.769471247018311, yes F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding domain protein [Arthrobacter phage Renna12]],,UXE05396,81.0811,1.08722E-128 SIF-HHPRED: Hypothetical Protein; Structural Genomics, NIAID, National Institute of Allergy and Infectious Diseases, Center for Structural Genomics of Infectious; HET: MSE, SO4; 1.8A {Vibrio cholerae O1 biovar El Tor},,,4RO3_B,21.1321,98.1 SIF-Syn: Shows solid synteny between AS3 phages.
    /note=Phages BLAST and frequency analysis agree on this Helix however HHPRED has no considerable contribution

CDS 26655 - 28058
  /gene="39"
  /product="gp39"
  /function="DNA methyltransferase"
  /locus tag="StuartMinion_39"
    /note=Original Glimmer call @bp 26655 has strength 11.71; Genemark calls start at 26655
    /note=SSC: 26655-28058 CP: yes SCS: both ST: SS BLAST-Start: [DNA methyltransferase [Arthrobacter phage Andrew] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.069, -4.864188174661353, no F: DNA methyltransferase SIF-BLAST: ,,[DNA methyltransferase [Arthrobacter phage Andrew] ],,YP_009815734,99.1435,0.0 SIF-HHPRED: c.66.1.11 (A:) m.PvuII N4 cytosine-specific DNA methyltransferase {Proteus vulgaris [TaxId: 585]} | CLASS: Alpha and beta proteins (a/b), FOLD: S-adenosyl-L-methionine-dependent methyltransferases, SUPFAM: S-adenosyl-L-methionine-dependent methyltransferases, FAM: Type II DNA methylase,,,SCOP_d1booa_,97.2163,99.9 SIF-Syn: Yes, this function shares synteny with other phages/ genes in its cluster.
    /note=This function is suported by the synteny and values in phagesdb function frequency, phagesdb BLAST, HHPRED and NCBI BLAST. This function is also known as DNA methylase.

CDS 28055 - 28516
  /gene="40"
  /product="gp40"
  /function="hypothetical protein"
  /locus tag="StuartMinion_40"
    /note=Original Glimmer call @bp 28055 has strength 9.47; Genemark calls start at 28061
    /note=SSC: 28055-28516 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein HOU53_gp49 [Arthrobacter phage Andrew] ],,NCBI, q1:s1 99.3464% 3.75577E-93 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.792, -5.092497832398658, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein HOU53_gp49 [Arthrobacter phage Andrew] ],,YP_009815735,95.4545,3.75577E-93 SIF-HHPRED: replisome organizer; helical  bipartite  natively unfolded domain, replication; HET: MSE; 2.4A {Bacillus phage SPP1} SCOP: l.1.1.1, a.179.1.1,,,1NO1_B,45.7516,99.3 SIF-Syn: Synteny with AS3
    /note=PhagesDB Function Frequency does not give any function for the AS3 subcluster. Every phage within the AS3 subcluster had called for an unknown function with the highest E-Value of 9e-77 (PhluffyCoco). NCBI Blast’s top accession: YP_009815735, shows one of the lowest E-Value out of all accessions of 3.75577e-93 and 99% coverage as well as high identity/alignment. This accession’s description calls for hypothetical protein.

CDS 28521 - 29105
  /gene="41"
  /product="gp41"
  /function="hypothetical protein"
  /locus tag="StuartMinion_41"
    /note=Original Glimmer call @bp 28521 has strength 9.47; Genemark calls start at 28476
    /note=SSC: 28521-29105 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein HOU53_gp50 [Arthrobacter phage Andrew] ],,NCBI, q1:s1 96.9072% 1.75708E-100 GAP: 4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.76, -3.0713502170045657, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein HOU53_gp50 [Arthrobacter phage Andrew] ],,YP_009815736,85.641,1.75708E-100 SIF-HHPRED: A2L_zn_ribbon ; A2L zinc ribbon domain,,,PF08792.15,15.9794,95.9 SIF-Syn: Synteny with other AS3 phages
    /note=There doesn’t appear to be a known function for this gene since there is no function noted for genes in the same pham according to Phagesdb.

CDS 29271 - 29498
  /gene="42"
  /product="gp42"
  /function="hypothetical protein"
  /locus tag="StuartMinion_42"
    /note=Original Glimmer call @bp 29271 has strength 9.15; Genemark calls start at 29229
    /note=SSC: 29271-29498 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein SEA_REDFOX_50 [Arthrobacter phage RedFox] ],,NCBI, q1:s1 100.0% 3.01924E-37 GAP: 165 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.281, -4.7230958680478965, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_REDFOX_50 [Arthrobacter phage RedFox] ],,WNM72837,90.6667,3.01924E-37 SIF-HHPRED: DUF6296 ; Family of unknown function (DUF6296),,,PF19813.4,22.6667,60.1 SIF-Syn: Synteny with other AS3 phages

CDS 29563 - 29718
  /gene="43"
  /product="gp43"
  /function="hypothetical protein"
  /locus tag="StuartMinion_43"
    /note=Original Glimmer call @bp 29563 has strength 13.84; Genemark calls start at 29563
    /note=SSC: 29563-29718 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_LEONA_50 [Arthrobacter phage Leona]],,NCBI, q1:s1 100.0% 8.44998E-5 GAP: 64 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.296, -2.0949393225970705, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_LEONA_50 [Arthrobacter phage Leona]],,QRI45458,94.1176,8.44998E-5 SIF-HHPRED: DUF4017 ; Protein of unknown function (DUF4017),,,PF13209.11,54.902,52.7 SIF-Syn: Synteny with other AS3 phages

CDS 29715 - 29963
  /gene="44"
  /product="gp44"
  /function="hypothetical protein"
  /locus tag="StuartMinion_44"
    /note=Original Glimmer call @bp 29724 has strength 10.61; Genemark calls start at 29715
    /note=SSC: 29715-29963 CP: yes SCS: both-gm ST: SS BLAST-Start: [hypothetical protein SEA_RENNA12_52 [Arthrobacter phage Renna12] ],,NCBI, q1:s1 98.7805% 1.40188E-43 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.92, -4.905314844093283, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_RENNA12_52 [Arthrobacter phage Renna12] ],,UXE05403,93.8272,1.40188E-43 SIF-HHPRED: b.82.1.20 (A:1-174) automated matches {Cupriavidus metallidurans [TaxId: 266264]} | CLASS: All beta proteins, FOLD: Double-stranded beta-helix, SUPFAM: RmlC-like cupins, FAM: 3-hydroxyanthranilic acid dioxygenase-like,,,SCOP_d6viaa1,76.8293,81.5 SIF-Syn: PhagesDB BLAST
Other phages that have high synteny in the same subcluster (AS3) have called for Function Unknown with similar E-Value and pham number (194220)
    /note=PhagesDB BLAST
    /note=AmiCi24 shows the function “Function Unknown” with a score of 142 and an E-Value of 4e-34
    /note=
    /note=NCBI-BLAST
    /note=QRI45460 shows the low E-Value at 1.47952e-40 and has a coverage of 98.7342% and has high identity/alignment at >85%

CDS 29956 - 30324
  /gene="45"
  /product="gp45"
  /function="nucleoside deoxyribosyltransferase"
  /locus tag="StuartMinion_45"
    /note=Original Glimmer call @bp 29935 has strength 9.54; Genemark calls start at 29956
    /note=SSC: 29956-30324 CP: yes SCS: both-gm ST: NI BLAST-Start: [nucleoside deoxyribosyltransferase [Arthrobacter phage Juno112]],,NCBI, q1:s1 95.9016% 2.88617E-70 GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.185, -4.271191715599192, no F: nucleoside deoxyribosyltransferase SIF-BLAST: ,,[nucleoside deoxyribosyltransferase [Arthrobacter phage Juno112]],,XIJ70449,90.2439,2.88617E-70 SIF-HHPRED: c.23.14.2 (A:1-118) Hypothetical protein PA1492 {Pseudomonas aeruginosa [TaxId: 287]} | CLASS: Alpha and beta proteins (a/b), FOLD: Flavodoxin-like, SUPFAM: N-(deoxy)ribosyltransferase-like, FAM: Hypothetical protein PA1492,,,SCOP_d1t1ja1,81.9672,99.7 SIF-Syn: PhluffyCoco has a high synteny with StuartMinion
    /note=Phages within subcluster AS3 called for this function with a 35% frequency

CDS 30321 - 30833
  /gene="46"
  /product="gp46"
  /function="SSB protein"
  /locus tag="StuartMinion_46"
    /note=Original Glimmer call @bp 30321 has strength 8.56; Genemark calls start at 30321
    /note=SSC: 30321-30833 CP: yes SCS: both ST: SS BLAST-Start: [SSB protein [Arthrobacter phage RedFox]],,NCBI, q1:s1 100.0% 2.54259E-91 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.628, -3.4283035164720754, yes F: SSB protein SIF-BLAST: ,,[SSB protein [Arthrobacter phage RedFox]],,WNM72842,93.4911,2.54259E-91 SIF-HHPRED: Single-stranded DNA-binding protein; DNA binding, quaternary structure, plasticity, inhibitor development, DNA BINDING PROTEIN; HET: FMT; 1.92A {Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)},,,7F5Y_A,70.0,100.0 SIF-Syn: Syntey with other AS3 phages
    /note=Phagesdb Function Frequency predicts ssdna binding protein

CDS 30988 - 31722
  /gene="47"
  /product="gp47"
  /function="NucT-like nuclease"
  /locus tag="StuartMinion_47"
    /note=Original Glimmer call @bp 30988 has strength 11.73; Genemark calls start at 30988
    /note=SSC: 30988-31722 CP: no SCS: both ST: SS BLAST-Start: [endonuclease [Arthrobacter phage Andrew] ],,NCBI, q1:s1 99.5902% 4.72509E-165 GAP: 154 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.648, -3.3060637489568596, yes F: NucT-like nuclease SIF-BLAST: ,,[endonuclease [Arthrobacter phage Andrew] ],,YP_009815744,95.9016,4.72509E-165 SIF-HHPRED: Mitochondrial cardiolipin hydrolase; piRNA, Phospholipase D, Nuclease, HYDROLASE; HET: EDO; 1.756A {Drosophila melanogaster},,,4GEL_A,56.5574,99.3 SIF-Syn: Synteny with AS3 phages
    /note=This function was determined using the NCBI blast table. This table shows NucT-like nuclease being the most probable function of the gene with an e-value o f4.72509e-165 which falls below the given limit of e^-7. This function is also supported by phagesDB, looking at the pham which is 152060, 43 of the 66 genes are noted down with a function of NucT-like nuclease. Additionally the HHPred table showcases an e-value of 1.1e-9 which further supports the function of the gene being RusA-like resolvase.

CDS 31723 - 32067
  /gene="48"
  /product="gp48"
  /function="terminase, small subunit"
  /locus tag="StuartMinion_48"
    /note=Original Glimmer call @bp 31723 has strength 8.55; Genemark calls start at 31723
    /note=SSC: 31723-32067 CP: yes SCS: both ST: NI BLAST-Start: [terminase small subunit [Arthrobacter phage RedFox]],,NCBI, q11:s16 91.2281% 6.55018E-64 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.98, -4.840756012219902, yes F: terminase, small subunit SIF-BLAST: ,,[terminase small subunit [Arthrobacter phage RedFox]],,WNM72844,84.8739,6.55018E-64 SIF-HHPRED: Terminase small subunit; genome packaging, bacteriophage, DNA binding, VIRAL PROTEIN; 1.4A {Enterobacteria phage HK97},,,6Z6E_A,83.3333,99.8 SIF-Syn: There is synteny in other subclusters with the same function,
    /note=HHPRED gives a good probability of 99.8% that the gene is a terminase, small subunit. Phagesdb Function Frequency shows many other subclusters genes having the same function. 
    /note=Phagesdb BLAST and NCBI BLAST also show high probability of this being the genes function

CDS 32064 - 32225
  /gene="49"
  /product="gp49"
  /function="hypothetical protein"
  /locus tag="StuartMinion_49"
    /note=Original Glimmer call @bp 32109 has strength 10.43
    /note=SSC: 32064-32225 CP: no SCS: glimmer-cs ST: SS BLAST-Start: [hypothetical protein HOU53_gp62 [Arthrobacter phage Andrew] ],,NCBI, q1:s1 100.0% 1.19527E-27 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.907, -2.7653702713535186, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein HOU53_gp62 [Arthrobacter phage Andrew] ],,YP_009815748,96.2264,1.19527E-27 SIF-HHPRED: LysW-like_globular ; Alpha-aminoadipate carrier protein LysW-like, globular domain,,,PF21344.2,18.8679,89.3 SIF-Syn: 
    /note=This gene only had 38 amino acids, making it very difficult for function to be discovered by platforms such as HHpred. It may be hypothesized that since the alignment consists of only 9 sequences, that causes a low probability that this protein matches another protein in the system.
    /note=According to the other genes in the same 221523 pham, this gene has an incredibly short length, at only 117 basepairs, without any function assigned.

CDS 32222 - 32347
  /gene="50"
  /product="gp50"
  /function="hypothetical protein"
  /locus tag="StuartMinion_50"
    /note=Original Glimmer call @bp 32222 has strength 7.43; Genemark calls start at 32222
    /note=SSC: 32222-32347 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_KHUMPHREY_60 [Arthrobacter phage KHumphrey] ],,NCBI, q4:s3 92.6829% 1.25105E-14 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.998, -4.741344217713455, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_KHUMPHREY_60 [Arthrobacter phage KHumphrey] ],,XIJ70225,90.0,1.25105E-14 SIF-HHPRED: DUF3649 ; Protein of unknown function (DUF3649),,,PF12365.13,29.2683,21.6 SIF-Syn: This gene exhibits synteny among phages within the same cluster.
    /note=NCBI BLAST, PhagesDB, and HHPRed data suggest the function of this gene is unknown.

CDS 32523 - 32750
  /gene="51"
  /product="gp51"
  /function="hypothetical protein"
  /locus tag="StuartMinion_51"
    /note=Original Glimmer call @bp 32526 has strength 7.99; Genemark calls start at 32526
    /note=SSC: 32523-32750 CP: yes SCS: both-cs ST: SS BLAST-Start: [hypothetical protein SEA_REDFOX_61 [Arthrobacter phage RedFox]],,NCBI, q2:s1 93.3333% 4.04253E-25 GAP: 175 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.648, -3.3060637489568596, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_REDFOX_61 [Arthrobacter phage RedFox]],,WNM72848,81.6901,4.04253E-25 SIF-HHPRED: DUF1290 ; Protein of unknown function (DUF1290),,,PF06947.17,32.0,32.0 SIF-Syn: Synteny with other AS3 phages
    /note=NCBI BLAST, PhagesDB, and HHPRed data suggest the function of this gene is unknown.

CDS 32747 - 33058
  /gene="52"
  /product="gp52"
  /function="hypothetical protein"
  /locus tag="StuartMinion_52"
    /note=Original Glimmer call @bp 32747 has strength 9.8; Genemark calls start at 32747
    /note=SSC: 32747-33058 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_REDFOX_62 [Arthrobacter phage RedFox]],,NCBI, q1:s1 100.0% 3.07215E-40 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.046, -4.702334767722353, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_REDFOX_62 [Arthrobacter phage RedFox]],,WNM72849,80.198,3.07215E-40 SIF-HHPRED: DUF3088 ; Protein of unknown function (DUF3088),,,PF11287.13,28.1553,50.9 SIF-Syn: There is synteny with similar phages in the same cluster; function unknown.
    /note=BLAST data from NCBI and Phagesdb indicate that the function for these gene/pham is unknown as well as HHPRED.

CDS 33046 - 33285
  /gene="53"
  /product="gp53"
  /function="hypothetical protein"
  /locus tag="StuartMinion_53"
    /note=Original Glimmer call @bp 33055 has strength 12.54; Genemark calls start at 33055
    /note=SSC: 33046-33285 CP: yes SCS: both-cs ST: NI BLAST-Start: [hypothetical protein SEA_REDFOX_63 [Arthrobacter phage RedFox]],,NCBI, q4:s1 81.0127% 4.14797E-28 GAP: -13 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.194, -4.393717312640863, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_REDFOX_63 [Arthrobacter phage RedFox]],,WNM72850,72.0,4.14797E-28 SIF-HHPRED: orf, hypothetical protein; a+b protein, Montreal-Kingston Bacterial Structural Genomics Initiative, BSGI, Structural Genomics, TRANSCRIPTION, PROTEIN BINDING; NMR {Escherichia coli} SCOP: b.137.1.2,,,1SG5_A,54.4304,73.8 SIF-Syn: 

CDS 33282 - 33491
  /gene="54"
  /product="gp54"
  /function="hypothetical protein"
  /locus tag="StuartMinion_54"
    /note=Original Glimmer call @bp 33282 has strength 14.48; Genemark calls start at 33282
    /note=SSC: 33282-33491 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein SEA_RENNA12_64 [Arthrobacter phage Renna12]],,NCBI, q2:s4 92.7536% 3.71276E-9 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.881, -4.905249357754755, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_RENNA12_64 [Arthrobacter phage Renna12]],,UXE05415,71.4286,3.71276E-9 SIF-HHPRED: DUF4350 ; Domain of unknown function (DUF4350),,,PF14258.11,69.5652,92.4 SIF-Syn: Synteny with other AS3 phages

CDS 33509 - 33676
  /gene="55"
  /product="gp55"
  /function="hypothetical protein"
  /locus tag="StuartMinion_55"
    /note=Original Glimmer call @bp 33488 has strength 5.89; Genemark calls start at 33488
    /note=SSC: 33509-33676 CP: no SCS: both-cs ST: NA BLAST-Start: [hypothetical protein SEA_LITTLETOKYO_64 [Arthrobacter phage LittleTokyo]],,NCBI, q6:s15 89.0909% 3.60114E-18 GAP: 17 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.165, -4.840589514456627, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_LITTLETOKYO_64 [Arthrobacter phage LittleTokyo]],,QGZ16956,70.3125,3.60114E-18 SIF-HHPRED: GEO11417p1; NADH:ubiquinone oxidoreductase, TRANSLOCASE, OXIDOREDUCTASE; HET: PC1, WSF, C14, ZMP, FMN, CDL, NDP, SF4, U10, 3PE, D12, DGT;{Drosophila melanogaster},,,8ESZ_B2,32.7273,69.5 SIF-Syn: Synteny with other AS phages
    /note=There doesn`t appear to be a known function for this gene since there is no function noted for genes in the same according to PhagesDB and HHPRED.

CDS 33673 - 33888
  /gene="56"
  /product="gp56"
  /function="hypothetical protein"
  /locus tag="StuartMinion_56"
    /note=Original Glimmer call @bp 33673 has strength 11.69; Genemark calls start at 33673
    /note=SSC: 33673-33888 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_RENNA12_66 [Arthrobacter phage Renna12]],,NCBI, q1:s1 100.0% 1.53744E-16 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.907, -2.8454123590742793, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_RENNA12_66 [Arthrobacter phage Renna12]],,UXE05417,65.7143,1.53744E-16 SIF-HHPRED: ZIP_Gal4-like_2; Leucine zipper Dimerization coil of Gal4-like transcription factors. The Gal4p family of transcriptional activators contain an N-terminal DNA-binding domain with a Zn2Cys6 binuclear cluster that interact with CCG triplets and a leucine zipper-like heptad repeat that dimerizes.,,,cd14725,29.5775,37.2 SIF-Syn: Has synteny with other phages in AS3, with similar base pair lengths.
    /note=PhagesDB and NCBI BLAST does not give any information on the function, either unknown function or hypothetical protein. The protein suggested by HHPRED have low data to be considered reliable.

CDS 33885 - 34013
  /gene="57"
  /product="gp57"
  /function="hypothetical protein"
  /locus tag="StuartMinion_57"
    /note=Original Glimmer call @bp 33885 has strength 2.22
    /note=SSC: 33885-34013 CP: yes SCS: glimmer ST: NI BLAST-Start: [hypothetical protein SEA_LEONA_65 [Arthrobacter phage Leona]],,NCBI, q1:s1 100.0% 8.66396E-17 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.436, -5.9139150158552, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_LEONA_65 [Arthrobacter phage Leona]],,QRI45473,90.4762,8.66396E-17 SIF-HHPRED: DUF1660 ; Prophage protein (DUF1660),,,PF07874.16,90.4762,71.3 SIF-Syn: Syntey with other AS3 phages

CDS 34010 - 34144
  /gene="58"
  /product="gp58"
  /function="hypothetical protein"
  /locus tag="StuartMinion_58"
    /note=Original Glimmer call @bp 34010 has strength 4.75
    /note=SSC: 34010-34144 CP: yes SCS: glimmer ST: SS BLAST-Start: [hypothetical protein SEA_REDFOX_68 [Arthrobacter phage RedFox]],,NCBI, q1:s1 100.0% 3.31485E-17 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.907, -2.9063687850157054, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_REDFOX_68 [Arthrobacter phage RedFox]],,WNM72855,88.8889,3.31485E-17 SIF-HHPRED: DUF1660 ; Prophage protein (DUF1660),,,PF07874.16,84.0909,97.7 SIF-Syn: Synteny with other AS3 pahges
    /note=The only piece of evidence to suggests the function is from HHPRED, which called for Prophage Protein. However, there is no other evidence to support this function, therefore, it can not be fully determine.

CDS 34182 - 34346
  /gene="59"
  /product="gp59"
  /function="hypothetical protein"
  /locus tag="StuartMinion_59"
    /note=Original Glimmer call @bp 34170 has strength 1.49
    /note=SSC: 34182-34346 CP: no SCS: glimmer-cs ST: NA BLAST-Start: [hypothetical protein SEA_LITTLETOKYO_70 [Arthrobacter phage LittleTokyo]],,NCBI, q26:s2 53.7037% 1.81022E-6 GAP: 37 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.552, -3.85780864677805, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_LITTLETOKYO_70 [Arthrobacter phage LittleTokyo]],,QGZ16962,83.3333,1.81022E-6 SIF-HHPRED: ELF ; ELF protein,,,PF03317.18,25.9259,37.3 SIF-Syn: Orpham
    /note=PhagesDB FF gives no function info for the AS3 subcluster, Every phage within the AS3 subcluster had called for an unknown function with the lowest E-Value of 2e-9 (Rattail_Draft) according to PhagesDB BLAST, HHPred`s top result shows a description of an ELF protein in the PF03317.18 hit with a 29% coverage, 25.4 probability and an E-Value of 170, NCBI BLAST`s top and only accession: QGZ16962, shows an e-value of 0.00000177094 and 50% coverage as well as high identity/alignment. This accession’s description calls for a hypothetical protein.

CDS 34325 - 34597
  /gene="60"
  /product="gp60"
  /function="helix-turn-helix DNA binding domain"
  /locus tag="StuartMinion_60"
    /note=Original Glimmer call @bp 34325 has strength 5.49; Genemark calls start at 34325
    /note=SSC: 34325-34597 CP: yes SCS: both ST: NI BLAST-Start: [helix-turn-helix DNA binding domain protein [Arthrobacter phage Renna12]],,NCBI, q11:s4 88.8889% 1.26187E-45 GAP: -22 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.907, -2.9063687850157054, yes F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding domain protein [Arthrobacter phage Renna12]],,UXE05422,93.9759,1.26187E-45 SIF-HHPRED: Recombination Directionality Factor RdfS; Excisionase, Recombination Directionality Factor, winged helix-turn-helix, superhelix, DNA BINDING PROTEIN; HET: GOL; 2.45A {Mesorhizobium japonicum R7A},,,8DGL_C,68.8889,99.2 SIF-Syn: When looking at the Phagesdb function frequency, the genes which are in the same 214403 pham and AS3 subcluster have been annotated to have the helix-turn-helix DNA binding domain.
    /note=NCBI blast, HHpred, and PhagesDb all support the function of helix-turn-helix binding domain as the function of gene 63 with good e-values and percentages.

CDS 34829 - 35122
  /gene="61"
  /product="gp61"
  /function="HNH endonuclease"
  /locus tag="StuartMinion_61"
    /note=
    /note=SSC: 34829-35122 CP: yes SCS: neither ST: NA BLAST-Start: [HNH endonuclease [Arthrobacter phage RedFox] ],,NCBI, q5:s3 95.8763% 8.35074E-57 GAP: 231 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.94, -4.924073590240254, yes F: HNH endonuclease SIF-BLAST: ,,[HNH endonuclease [Arthrobacter phage RedFox] ],,WNM72858,92.6316,8.35074E-57 SIF-HHPRED: CRISPR-associated endonuclease Cas9; Inhibitor, Complex, VIRAL PROTEIN; HET: NI; 2.097A {Staphylococcus aureus},,,7ENH_A,73.1959,98.3 SIF-Syn: Synteny with other AS phages