CDS 55 - 444 /gene="1" /product="gp1" /function="terminase, small subunit" /locus tag="Venti_1" /note=Original Glimmer call @bp 55 has strength 13.97; Genemark calls start at 151 /note=SSC: 55-444 CP: yes SCS: both-gl ST: SS BLAST-Start: [terminase small subunit [Mycobacterium phage Bartholomew] ],,NCBI, q1:s1 100.0% 5.9599E-88 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.557, -5.79598068500287, no F: terminase, small subunit SIF-BLAST: ,,[terminase small subunit [Mycobacterium phage Bartholomew] ],,YP_009964726,100.0,5.9599E-88 SIF-HHPRED: Terminase small subunit; genome packaging, bacteriophage, DNA binding, VIRAL PROTEIN; 1.4A {Enterobacteria phage HK97},,,6Z6E_B,76.7442,99.7 SIF-Syn: /note=Glimmer and Genemark do not match one another. NCBI blast had many 1:1 which was a good thing to see. Along with that, the pages DB and HHPRED both agreed that this was a small subunit, terminase. CDS 413 - 2089 /gene="2" /product="gp2" /function="terminase, large subunit" /locus tag="Venti_2" /note=Original Glimmer call @bp 413 has strength 15.37; Genemark calls start at 629 /note=SSC: 413-2089 CP: yes SCS: both-gl ST: NI BLAST-Start: [terminase large subunit [Mycobacterium phage Shipwreck] ],,NCBI, q1:s1 100.0% 0.0 GAP: -32 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.867, -3.949209453443199, yes F: terminase, large subunit SIF-BLAST: ,,[terminase large subunit [Mycobacterium phage Shipwreck] ],,YP_009303760,99.8208,0.0 SIF-HHPRED: Terminase large subunit; genome packaging, bacteriophage, ATPase, nuclease, VIRAL PROTEIN; HET: BR; 2.2A {Enterobacteria phage HK97},,,6Z6D_A,82.0788,100.0 SIF-Syn: /note=Glimmer and GeneMark start sites are very off. In starterator, it is part of the genes that do not have the "most annotated" start so it`s not very informative to us. Both NCBI BLAST and HHPRED gave the same function of terminase large subunits and a small subunit. For NCBI blast there are a lot of 1:1 ratios and percent coverage. CDS 2106 - 3560 /gene="3" /product="gp3" /function="portal protein" /locus tag="Venti_3" /note=Original Glimmer call @bp 2106 has strength 12.88; Genemark calls start at 2148 /note=SSC: 2106-3560 CP: no SCS: both-gl ST: NA BLAST-Start: [portal protein [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 0.0 GAP: 16 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.293, -2.356391881054909, yes F: portal protein SIF-BLAST: ,,[portal protein [Mycobacterium phage Fishburne] ],,YP_008051228,100.0,0.0 SIF-HHPRED: SIF-Syn: Follows terminase /note=GeneMark and Glimmer don`t agree, but based on the evidence given, I think Glimmer is the correct start site. CDS 3551 - 4888 /gene="4" /product="gp4" /function="capsid maturation protease" /locus tag="Venti_4" /note=Original Glimmer call @bp 3551 has strength 18.43; Genemark calls start at 3551 /note=SSC: 3551-4888 CP: yes SCS: both ST: SS BLAST-Start: [ATP-dependent Clp protease proteolytic subunit [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 0.0 GAP: -10 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.762, -3.9797067239234645, no F: capsid maturation protease SIF-BLAST: ,,[ATP-dependent Clp protease proteolytic subunit [Mycobacterium phage Fishburne] ],,YP_008051229,100.0,0.0 SIF-HHPRED: c.14.1.1 (A:) Clp protease, ClpP subunit {Helicobacter pylori [TaxId: 210]},,,d2zl4a_,36.8539,99.9 SIF-Syn: Follows portal protein /note=This particular gene has a good NCBI blast match with many 1:1 matches. The HHPRED however calls it a capsid maturation protease with little coverage and a high probability. We made sure to go through some pages to ensure that they were all being called that and they were. This led us to also calling it a capsid maturation protease. CDS 4892 - 5257 /gene="5" /product="gp5" /function="scaffolding protein" /locus tag="Venti_5" /note=Original Glimmer call @bp 4892 has strength 14.08; Genemark calls start at 4892 /note=SSC: 4892-5257 CP: yes SCS: both ST: SS BLAST-Start: [scaffolding protein [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 2.11656E-75 GAP: 3 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.209, -2.583959800616441, yes F: scaffolding protein SIF-BLAST: ,,[scaffolding protein [Mycobacterium phage Fishburne] ],,YP_008051230,100.0,2.11656E-75 SIF-HHPRED: SIF-Syn: Follows capsid maturation protease /note=NCBI and HHPREd gave different functions. NCBI Blast gave a lot of 1:1 ratios and coverage is good. HHPRED was not helpful with function information because it was inconsistent, with coverage of around 80-99%. Phages DB and NCBI blast give the same function info. Longest ORF, gap is okay. Starterator informative. CDS 5294 - 6238 /gene="6" /product="gp6" /function="major capsid protein" /locus tag="Venti_6" /note=Original Glimmer call @bp 5294 has strength 17.73; Genemark calls start at 5294 /note=SSC: 5294-6238 CP: yes SCS: both ST: SS BLAST-Start: [major capsid protein [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 0.0 GAP: 36 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.57, -3.7091199845487814, no F: major capsid protein SIF-BLAST: ,,[major capsid protein [Mycobacterium phage Fishburne] ],,YP_008051231,100.0,0.0 SIF-HHPRED: d.183.1.1 (A:104-383) Major capsid protein gp5 {Bacteriophage HK97 [TaxId: 37554]},,,d2fsya1,92.3567,99.8 SIF-Syn: Follows scaffolding protein CDS 6238 - 6588 /gene="7" /product="gp7" /function="hypothetical protein" /locus tag="Venti_7" /note=Original Glimmer call @bp 6238 has strength 14.42; Genemark calls start at 6238 /note=SSC: 6238-6588 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_FISHBURNE_7 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 8.91944E-76 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.437, -4.623784469830713, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_7 [Mycobacterium phage Fishburne] ],,YP_008051232,100.0,8.91944E-76 SIF-HHPRED: SIF-Syn: /note=According to the HHPRED there were two hypothetical proteins that could have been called, however, there just was not enough information to call it. Along with little evidence on HHPRED there were no functions that were called on pages DB. CDS 6592 - 6954 /gene="8" /product="gp8" /function="head-to-tail adaptor" /locus tag="Venti_8" /note=Original Glimmer call @bp 6592 has strength 9.48; Genemark calls start at 6592 /note=SSC: 6592-6954 CP: yes SCS: both ST: SS BLAST-Start: [head-to-tail adaptor [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 3.78034E-81 GAP: 3 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.131, -4.657213748971, no F: head-to-tail adaptor SIF-BLAST: ,,[head-to-tail adaptor [Mycobacterium phage Fishburne] ],,YP_008051233,100.0,3.78034E-81 SIF-HHPRED: SIF-Syn: CDS 6954 - 7304 /gene="9" /product="gp9" /function="head-to-tail stopper" /locus tag="Venti_9" /note=Original Glimmer call @bp 6954 has strength 17.4; Genemark calls start at 6954 /note=SSC: 6954-7304 CP: yes SCS: both ST: NI BLAST-Start: [head-to-tail stopper [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 1.8002E-79 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.736, -5.711596282398514, no F: head-to-tail stopper SIF-BLAST: ,,[head-to-tail stopper [Mycobacterium phage Fishburne] ],,YP_008051234,100.0,1.8002E-79 SIF-HHPRED: Phage_attach ; Phage Head-Tail Attachment,,,PF05354.14,99.1379,94.3 SIF-Syn: /note=Doesn`t have the best SD sequence but all other evidence points to this being the correct start site. Coding potential ends before the stop site. CDS 7319 - 7663 /gene="10" /product="gp10" /function="hypothetical protein" /locus tag="Venti_10" /note=Original Glimmer call @bp 7319 has strength 15.18; Genemark calls start at 7319 /note=SSC: 7319-7663 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_FISHBURNE_10 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 2.91144E-74 GAP: 14 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.467, -2.58321678164666, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_10 [Mycobacterium phage Fishburne] ],,YP_008051235,100.0,2.91144E-74 SIF-HHPRED: SIF-Syn: /note=Not the longest ORF. PhagesDB is not helpful for function. On starterator, in the most annotated list. A lot of other P1 proteins went with the suggested start site. According to NCBI Blast it is a hypothetical protein, not called yet because there`s not enough information. HHPred gives inconsistent function and very low coverage. CDS 7663 - 7932 /gene="11" /product="gp11" /function="hypothetical protein" /locus tag="Venti_11" /note=Original Glimmer call @bp 7663 has strength 12.5; Genemark calls start at 7663 /note=SSC: 7663-7932 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_FISHBURNE_11 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 1.14897E-56 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.838, -5.238109312333945, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_11 [Mycobacterium phage Fishburne] ],,YP_008051236,100.0,1.14897E-56 SIF-HHPRED: SIF-Syn: /note=There was not enough information to call this a protein. According to the HHPRED there were some that could have possibly been proteins, however, there was very little coverage and a lower percentage. Phages DB also had similar results that called no function, therefore it not being called. CDS 7932 - 8309 /gene="12" /product="gp12" /function="hypothetical protein" /locus tag="Venti_12" /note=Original Glimmer call @bp 7932 has strength 14.42; Genemark calls start at 7932 /note=SSC: 7932-8309 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein PBI_FISHBURNE_12 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 6.04163E-86 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.338, -4.1673872239336225, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_12 [Mycobacterium phage Fishburne] ],,YP_008051237,100.0,6.04163E-86 SIF-HHPRED: Uncharacterized protein Rv2865; heterotetramer, 1:1 ratio, Structural Genomics, PSI-2, Protein Structure Initiative, TB Structural Genomics Consortium, TBSGC, Integrated Center; 2.0A {Mycobacterium tuberculosis},,,3G5O_D,20.0,55.1 SIF-Syn: /note=Not the best SD score or the longest ORF but all other evidence points to this being the correct start site. CDS 8306 - 8743 /gene="13" /product="gp13" /function="tail terminator" /locus tag="Venti_13" /note=Original Glimmer call @bp 8306 has strength 13.49; Genemark calls start at 8306 /note=SSC: 8306-8743 CP: yes SCS: both ST: NI BLAST-Start: [tail terminator [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 1.06518E-103 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.884, -3.147993589957652, yes F: tail terminator SIF-BLAST: ,,[tail terminator [Mycobacterium phage Fishburne] ],,YP_008051238,100.0,1.06518E-103 SIF-HHPRED: Tail terminator protein Rcc01690; "neck", "portal", "capsid", "tail tube", VIRUS; 3.58A {Rhodobacter capsulatus},,,6TE9_F,93.1034,98.9 SIF-Syn: /note=Longest ORF. Starterator is not helpful. Gene is not listed as most annotated. NCBI blast gives good 1:1 ratios and probability. CDS 8834 - 9832 /gene="14" /product="gp14" /function="major tail protein" /locus tag="Venti_14" /note=Original Glimmer call @bp 8834 has strength 18.24; Genemark calls start at 8834 /note=SSC: 8834-9832 CP: no SCS: both ST: SS BLAST-Start: [major tail subunit [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 0.0 GAP: 90 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.456, -2.0949393225970705, yes F: major tail protein SIF-BLAST: ,,[major tail subunit [Mycobacterium phage Fishburne] ],,YP_008051239,100.0,0.0 SIF-HHPRED: YSD1_22 major tail protein; Bacteriophage tail, helical assembly, VIRAL PROTEIN; 3.5A {Bacteriophage sp.},,,6XGR_M,42.1687,98.4 SIF-Syn: CDS 9941 - 10540 /gene="15" /product="gp15" /function="tail assembly chaperone" /locus tag="Venti_15" /note=Original Glimmer call @bp 9941 has strength 18.84; Genemark calls start at 9941 /note=SSC: 9941-10540 CP: yes SCS: both ST: NI BLAST-Start: [tail assembly chaperone [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 2.37192E-145 GAP: 108 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.946, -3.0248543014571307, yes F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Mycobacterium phage Fishburne] ],,YP_008051241,100.0,2.37192E-145 SIF-HHPRED: GP24_25 ; Mycobacteriophage tail assembly protein,,,PF17388.5,32.1608,95.0 SIF-Syn: CDS join(9941..10531,10533..10859) /gene="16" /product="gp16" /function="tail assembly chaperone" /locus tag="Venti_16" /note= /note=SSC: 9941-10859 CP: yes SCS: neither ST: NI BLAST-Start: [tail assembly chaperone [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 0.0 GAP: -600 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.946, -3.0248543014571307, yes F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Mycobacterium phage Fishburne] ],,YP_008051240,100.0,0.0 SIF-HHPRED: SIF-Syn: CDS 10873 - 14643 /gene="17" /product="gp17" /function="tape measure protein" /locus tag="Venti_17" /note=Original Glimmer call @bp 10873 has strength 13.76; Genemark calls start at 10873 /note=SSC: 10873-14643 CP: yes SCS: both ST: SS BLAST-Start: [tape measure protein [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 0.0 GAP: 14 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.836, -3.3226484487933954, no F: tape measure protein SIF-BLAST: ,,[tape measure protein [Mycobacterium phage Fishburne] ],,YP_008051242,100.0,0.0 SIF-HHPRED: Tape Measure Protein, gp57; phage tail, tail tip, tape measure protein, VIRAL PROTEIN; 3.7A {Staphylococcus virus 80alpha},,,6V8I_AF,39.5701,93.3 SIF-Syn: /note=There wasn`t very much information that was given however with the HHPRED there was evidence of tape measure and phages DB also called tape measure protein. This was also the longest in the whole entire genome confirming that this was in a tape measure protein. CDS 14640 - 16385 /gene="18" /product="gp18" /function="minor tail protein" /locus tag="Venti_18" /note=Original Glimmer call @bp 14640 has strength 13.67; Genemark calls start at 14640 /note=SSC: 14640-16385 CP: yes SCS: both ST: NA BLAST-Start: [minor tail protein [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.952, -3.780848860724383, yes F: minor tail protein SIF-BLAST: ,,[minor tail protein [Mycobacterium phage Fishburne] ],,YP_008051243,100.0,0.0 SIF-HHPRED: SIF-Syn: Follows tape measure protein /note=Gene in a list of genes without the most annotated start. HHPRED did not give good coverage, the function is inconsistent. PhagesDB and NCBI blast gives the same function. Probability is good on NCBI Blast CDS 16385 - 18121 /gene="19" /product="gp19" /function="minor tail protein" /locus tag="Venti_19" /note=Original Glimmer call @bp 16385 has strength 18.27; Genemark calls start at 16385 /note=SSC: 16385-18121 CP: yes SCS: both ST: NI BLAST-Start: [minor tail protein [Mycobacterium phage Brusacoram] ],,NCBI, q1:s1 100.0% 0.0 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.006, -4.966271270034708, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Mycobacterium phage Brusacoram] ],,YP_009193916,99.827,0.0 SIF-HHPRED: SIF-Syn: /note=Not the best SD score or longest ORF but all other evidence points to this being the correct start site. CDS 18144 - 19037 /gene="20" /product="gp20" /function="minor tail protein" /locus tag="Venti_20" /note=Original Glimmer call @bp 18144 has strength 15.86; Genemark calls start at 18144 /note=SSC: 18144-19037 CP: yes SCS: both ST: NA BLAST-Start: [minor tail protein [Mycobacterium phage Shipwreck] ],,NCBI, q1:s1 100.0% 0.0 GAP: 22 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.752, -3.875461547814295, yes F: minor tail protein SIF-BLAST: ,,[minor tail protein [Mycobacterium phage Shipwreck] ],,YP_009303777,99.6633,0.0 SIF-HHPRED: SIF-Syn: CDS 19038 - 21299 /gene="21" /product="gp21" /function="minor tail protein" /locus tag="Venti_21" /note=Original Glimmer call @bp 19038 has strength 14.12; Genemark calls start at 19038 /note=SSC: 19038-21299 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Mycobacterium phage Arib1] ],,NCBI, q1:s1 100.0% 0.0 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.825, -5.53477478450612, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Mycobacterium phage Arib1] ],,YP_009964905,99.6016,0.0 SIF-HHPRED: SIF-Syn: /note=Not the best SD sequence but all other evidence points to this being the correct start site, BLASTs say that this is a minor tail protein but the gene I did before this one was also a minor tail protein? Is that possible? CDS 21299 - 22336 /gene="22" /product="gp22" /function="minor tail protein" /locus tag="Venti_22" /note=Original Glimmer call @bp 21299 has strength 9.27; Genemark calls start at 21299 /note=SSC: 21299-22336 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein PBI_FISHBURNE_22 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 0.0 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.006, -5.87535162071584, no F: minor tail protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_22 [Mycobacterium phage Fishburne] ],,YP_008051247,100.0,0.0 SIF-HHPRED: SIF-Syn: /note=HHPRED percent coverage is not good. Phagesdb blast and NCBI blast give minor tail protein as functions. Not the longest ORF but the gap is good. The longest ORF has a different start from glimmer and genemark. CDS 22351 - 22500 /gene="23" /product="gp23" /function="Hypothetical Protein" /locus tag="Venti_23" /note=Original Glimmer call @bp 22351 has strength 6.08 /note=SSC: 22351-22500 CP: yes SCS: glimmer ST: NA BLAST-Start: [hypothetical protein PBI_FISHBURNE_23 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 4.5401E-26 GAP: 14 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.293, -2.417348306996335, yes F: Hypothetical Protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_23 [Mycobacterium phage Fishburne] ],,YP_008051248,100.0,4.5401E-26 SIF-HHPRED: SIF-Syn: /note=Genemark start not given. Start given by Glimmer is not the longest ORF. Phagesdb didn`t give any info. CDS 22517 - 22879 /gene="24" /product="gp24" /function="hypothetical protein" /locus tag="Venti_24" /note=Original Glimmer call @bp 22517 has strength 10.16; Genemark calls start at 22517 /note=SSC: 22517-22879 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein PBI_FISHBURNE_24 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 1.02656E-80 GAP: 16 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.54, -4.295041304450046, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_24 [Mycobacterium phage Fishburne] ],,YP_008051249,100.0,1.02656E-80 SIF-HHPRED: SIF-Syn: /note=There weren`t very many matches that were on HHPRED and the matches that did come up had very little coverage with love possibility which was an indication of no function. Along with that there on phages DB they also agreed that there was not a function present. CDS 22879 - 23049 /gene="25" /product="gp25" /function="membrane protein" /locus tag="Venti_25" /note=Original Glimmer call @bp 22879 has strength 17.31; Genemark calls start at 22876 /note=SSC: 22879-23049 CP: yes SCS: both-gl ST: NA BLAST-Start: [hypothetical protein PBI_FISHBURNE_25 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 5.50162E-31 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.623, -5.5843640798556455, no F: membrane protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_25 [Mycobacterium phage Fishburne] ],,YP_008051250,100.0,5.50162E-31 SIF-HHPRED: SIF-Syn: /note=We called this a membrane protein it was matched with good quality coverage on the NCBI blasts. They also called this a membrane protein. There was also evidence that this was a membrane protein from the TmHmm. Leading us to make the final call of membrane protein. CDS 23120 - 24055 /gene="26" /product="gp26" /function="lysin A" /locus tag="Venti_26" /note=Original Glimmer call @bp 23120 has strength 15.58; Genemark calls start at 23120 /note=SSC: 23120-24055 CP: yes SCS: both ST: SS BLAST-Start: [lysin A [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 0.0 GAP: 70 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.138, -2.9334385989924945, yes F: lysin A SIF-BLAST: ,,[lysin A [Mycobacterium phage Fishburne] ],,YP_008051251,100.0,0.0 SIF-HHPRED: General Secretion Pathway Protein; Type 2 Secretion System protein, Peptidoglycan-binding protein; 1.55A {Vibrio vulnificus},,,4G54_A,23.4727,96.5 SIF-Syn: /note=Starterator called it as the most annotated/suggested start site. CDS 24052 - 25218 /gene="27" /product="gp27" /function="lysin B" /locus tag="Venti_27" /note=Original Glimmer call @bp 24052 has strength 12.14; Genemark calls start at 24052 /note=SSC: 24052-25218 CP: yes SCS: both ST: NA BLAST-Start: [lysin B [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.401, -4.123435721190396, no F: lysin B SIF-BLAST: ,,[lysin B [Mycobacterium phage Fishburne] ],,YP_008051252,100.0,0.0 SIF-HHPRED: Gene 12 protein; alpha/beta sandwich, CELL ADHESION; 2.0A {Mycobacterium phage D29},,,3HC7_A,71.9072,100.0 SIF-Syn: /note=Starterator did not have as most annotated start site. CDS 25240 - 25488 /gene="28" /product="gp28" /function="holin" /locus tag="Venti_28" /note=Original Glimmer call @bp 25240 has strength 16.49; Genemark calls start at 25240 /note=SSC: 25240-25488 CP: yes SCS: both ST: SS BLAST-Start: [holin [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 6.35085E-48 GAP: 21 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.671, -3.649482460397077, yes F: holin SIF-BLAST: ,,[holin [Mycobacterium phage Fishburne] ],,YP_008051253,100.0,6.35085E-48 SIF-HHPRED: Phage_r1t_holin ; Putative lactococcus lactis phage r1t holin,,,PF16945.8,86.5854,99.9 SIF-Syn: CDS 25485 - 25844 /gene="29" /product="gp29" /function="hypothetical protein" /locus tag="Venti_29" /note=Original Glimmer call @bp 25485 has strength 15.86; Genemark calls start at 25485 /note=SSC: 25485-25844 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_FISHBURNE_29 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 5.13911E-78 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.101, -4.778071418780228, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_29 [Mycobacterium phage Fishburne] ],,YP_008051254,100.0,5.13911E-78 SIF-HHPRED: DUF2746 ; Protein of unknown function (DUF2746),,,PF10874.11,90.7563,99.9 SIF-Syn: CDS complement (25919 - 26875) /gene="30" /product="gp30" /function="tyrosine integrase" /locus tag="Venti_30" /note=Original Glimmer call @bp 26875 has strength 12.42; Genemark calls start at 26812 /note=SSC: 26875-25919 CP: yes SCS: both-gl ST: NA BLAST-Start: [integrase [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 0.0 GAP: 70 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.589, -3.670670413150579, no F: tyrosine integrase SIF-BLAST: ,,[integrase [Mycobacterium phage Fishburne] ],,YP_008051255,100.0,0.0 SIF-HHPRED: Gp33; Bacteriophage, Brujita, DNA-binding, Integrase, DNA BINDING PROTEIN; HET: ACT, GOL; 1.847A {Mycobacterium phage Brujita},,,5VFZ_A,98.1132,100.0 SIF-Syn: /note=Do not have most annotated start, Starterator not applicable. CDS complement (26946 - 27347) /gene="31" /product="gp31" /function="immunity repressor" /locus tag="Venti_31" /note=Original Glimmer call @bp 27347 has strength 0.7; Genemark calls start at 27347 /note=SSC: 27347-26946 CP: yes SCS: both ST: SS BLAST-Start: [immunity repressor [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 1.84857E-92 GAP: 116 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.711, -5.9375763810740265, no F: immunity repressor SIF-BLAST: ,,[immunity repressor [Mycobacterium phage Fishburne] ],,YP_008051256,100.0,1.84857E-92 SIF-HHPRED: SIF-Syn: CDS 27464 - 27709 /gene="32" /product="gp32" /function="hypothetical protein" /locus tag="Venti_32" /note=Original Glimmer call @bp 27464 has strength 10.13; Genemark calls start at 27464 /note=SSC: 27464-27709 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein PBI_FISHBURNE_32 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 2.00836E-49 GAP: 116 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.292, -6.302157836014727, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_32 [Mycobacterium phage Fishburne] ],,YP_008051257,100.0,2.00836E-49 SIF-HHPRED: SIF-Syn: /note=Has the most annotated start, but does not call it. CDS 27706 - 27942 /gene="33" /product="gp33" /function="hypothetical protein" /locus tag="Venti_33" /note=Original Glimmer call @bp 27706 has strength 11.36; Genemark calls start at 27706 /note=SSC: 27706-27942 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein PBI_FISHBURNE_33 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 1.05285E-44 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.63, -5.649978513542845, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_33 [Mycobacterium phage Fishburne] ],,YP_008051258,100.0,1.05285E-44 SIF-HHPRED: SIF-Syn: /note=Does not have the most annotated start, and does not call it. CDS 27939 - 28805 /gene="34" /product="gp34" /function="antirepressor" /locus tag="Venti_34" /note=Original Glimmer call @bp 27939 has strength 12.49; Genemark calls start at 27939 /note=SSC: 27939-28805 CP: yes SCS: both ST: SS BLAST-Start: [antirepressor [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.077, -6.746778599452446, no F: antirepressor SIF-BLAST: ,,[antirepressor [Mycobacterium phage Fishburne] ],,YP_008051259,100.0,0.0 SIF-HHPRED: ANT ; Phage antirepressor protein KilAC domain,,,PF03374.17,37.5,98.5 SIF-Syn: /note=Starterator called as suggested/most annotated start site. CDS 28802 - 29101 /gene="35" /product="gp35" /function="hypothetical protein" /locus tag="Venti_35" /note=Original Glimmer call @bp 28802 has strength 18.21; Genemark calls start at 28802 /note=SSC: 28802-29101 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_FISHBURNE_35 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 3.81221E-59 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.672, -3.6476983004329377, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_35 [Mycobacterium phage Fishburne] ],,YP_008051260,100.0,3.81221E-59 SIF-HHPRED: SIF-Syn: /note=Has and calls the most annotated start. CDS 29098 - 29346 /gene="36" /product="gp36" /function="hypothetical protein" /locus tag="Venti_36" /note=Original Glimmer call @bp 29098 has strength 14.92; Genemark calls start at 29098 /note=SSC: 29098-29346 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_FISHBURNE_36 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 3.20338E-49 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.224, -4.535723274102247, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_36 [Mycobacterium phage Fishburne] ],,YP_008051261,100.0,3.20338E-49 SIF-HHPRED: SIF-Syn: /note=Starterator called as suggested/most annotated start site. CDS 29343 - 29744 /gene="37" /product="gp37" /function="hypothetical protein" /locus tag="Venti_37" /note=Original Glimmer call @bp 29343 has strength 17.66; Genemark calls start at 29343 /note=SSC: 29343-29744 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_FISHBURNE_37 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 2.44483E-90 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.006, -4.905314844093283, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_37 [Mycobacterium phage Fishburne] ],,YP_008051262,100.0,2.44483E-90 SIF-HHPRED: SIF-Syn: /note=Has and calls the most annotated start. CDS 29807 - 30310 /gene="38" /product="gp38" /function="hypothetical protein" /locus tag="Venti_38" /note=Original Glimmer call @bp 29807 has strength 12.82; Genemark calls start at 29807 /note=SSC: 29807-30310 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein PBI_FISHBURNE_38 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 4.45858E-115 GAP: 62 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.519, -4.3372389842445, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_38 [Mycobacterium phage Fishburne] ],,YP_008051263,100.0,4.45858E-115 SIF-HHPRED: DUF732 ; Protein of unknown function (DUF732),,,PF05305.17,41.9162,99.5 SIF-Syn: /note=Starterator does not have it as the most annotated/suggested start site. CDS 30310 - 30543 /gene="39" /product="gp39" /function="hypothetical protein" /locus tag="Venti_39" /note=Original Glimmer call @bp 30310 has strength 5.46; Genemark calls start at 30310 /note=SSC: 30310-30543 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_FISHBURNE_39 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 6.08875E-49 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.762, -4.234979229026771, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_39 [Mycobacterium phage Fishburne] ],,YP_008051264,100.0,6.08875E-49 SIF-HHPRED: SIF-Syn: /note=Has and calls the most annotated start. CDS 30540 - 30815 /gene="40" /product="gp40" /function="hypothetical protein" /locus tag="Venti_40" /note=Original Glimmer call @bp 30540 has strength 14.47; Genemark calls start at 30540 /note=SSC: 30540-30815 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_FISHBURNE_40 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 9.73221E-58 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.71, -5.7625728666356775, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_40 [Mycobacterium phage Fishburne] ],,YP_008051265,100.0,9.73221E-58 SIF-HHPRED: SIF-Syn: /note=Starterator calls as most annotated/suggested start site. CDS 30812 - 31084 /gene="41" /product="gp41" /function="hypothetical protein" /locus tag="Venti_41" /note=Original Glimmer call @bp 30812 has strength 12.06; Genemark calls start at 30812 /note=SSC: 30812-31084 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_FISHBURNE_41 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 1.48948E-59 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.006, -4.905314844093283, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_41 [Mycobacterium phage Fishburne] ],,YP_008051266,100.0,1.48948E-59 SIF-HHPRED: SIF-Syn: /note=Has and calls the most annotated start. CDS 31081 - 31260 /gene="42" /product="gp42" /function="hypothetical protein" /locus tag="Venti_42" /note=Original Glimmer call @bp 31081 has strength 11.69; Genemark calls start at 31081 /note=SSC: 31081-31260 CP: yes SCS: both ST: SS BLAST-Start: [gp43 [Mycobacterium phage Brujita] ],,NCBI, q1:s1 100.0% 1.91418E-35 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.006, -5.3524728754355015, yes F: hypothetical protein SIF-BLAST: ,,[gp43 [Mycobacterium phage Brujita] ],,YP_002242026,100.0,1.91418E-35 SIF-HHPRED: FYDLN_acid ; Protein of unknown function (FYDLN_acid),,,PF09538.13,98.3051,91.0 SIF-Syn: /note=Starterator calls as most annotated/suggested start site. CDS 31257 - 31388 /gene="43" /product="gp43" /function="hypothetical protein" /locus tag="Venti_43" /note=Original Glimmer call @bp 31257 has strength 5.91 /note=SSC: 31257-31388 CP: yes SCS: glimmer ST: SS BLAST-Start: [gp44 [Mycobacterium phage Brujita] ],,NCBI, q1:s1 100.0% 1.41233E-23 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.676, -5.542591178556752, no F: hypothetical protein SIF-BLAST: ,,[gp44 [Mycobacterium phage Brujita] ],,YP_002242027,100.0,1.41233E-23 SIF-HHPRED: SIF-Syn: /note=There are two distinct peaks of coding potential in the gene 43 ORF, but spaced between those two peaks is gene 42 in a completely different frame. This pattern was not repeated in the GeneMarkS. Also has and calls the most annotated start. CDS 31385 - 31471 /gene="44" /product="gp44" /function="hypothetical protein" /locus tag="Venti_44" /note=Original Glimmer call @bp 31349 has strength 2.77 /note=SSC: 31385-31471 CP: yes SCS: glimmer-cs ST: NI BLAST-Start: [hypothetical protein I5J45_gp43 [Mycobacterium phage Bartholomew] ],,NCBI, q1:s1 100.0% 3.53477E-10 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.589, -4.197870532213559, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein I5J45_gp43 [Mycobacterium phage Bartholomew] ],,YP_009964768,100.0,3.53477E-10 SIF-HHPRED: SIF-Syn: /note=There is no coding potential on the Host-Trained GeneMark. However, there is a small amount of coding potential displayed on GeneMarkS. Switched the start site for a smaller overlap. Also, did not choose the automated start site because it was a large overlap. I switched the start site because the overlap was shorter and it has the optimal base pair overlap. CDS 31468 - 31620 /gene="45" /product="gp45" /function="hypothetical protein" /locus tag="Venti_45" /note=Original Glimmer call @bp 31471 has strength 18.0; Genemark calls start at 31468 /note=SSC: 31468-31620 CP: no SCS: both-gm ST: NA BLAST-Start: [hypothetical protein SEA_ZILIZEBETH_45 [Mycobacterium phage Zilizebeth]],,NCBI, q1:s1 100.0% 1.04709E-26 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.248, -4.487636275856737, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ZILIZEBETH_45 [Mycobacterium phage Zilizebeth]],,QBI97979,100.0,1.04709E-26 SIF-HHPRED: SIF-Syn: /note=There is little evidence on HHPRED that leads us to believe there is a function that can be called. CDS 31617 - 32546 /gene="46" /product="gp46" /function="exonuclease" /locus tag="Venti_46" /note=Original Glimmer call @bp 31617 has strength 12.89; Genemark calls start at 31617 /note=SSC: 31617-32546 CP: yes SCS: both ST: NI BLAST-Start: [RecE [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.497, -6.3610730471974195, no F: exonuclease SIF-BLAST: ,,[RecE [Mycobacterium phage Fishburne] ],,YP_008051270,100.0,0.0 SIF-HHPRED: Exodeoxyribonuclease 8; Exonuclease, Recombination, Hydrolase, Nuclease; 2.8A {Escherichia coli},,,3H4R_A,87.055,99.9 SIF-Syn: /note=agreement that function is exonuclease, but RecE-like and RecB-like are both chosen without clear evidence CDS 32543 - 33535 /gene="47" /product="gp47" /function="RecT-like ssDNA binding protein" /locus tag="Venti_47" /note=Original Glimmer call @bp 32543 has strength 14.26; Genemark calls start at 32543 /note=SSC: 32543-33535 CP: yes SCS: both ST: NA BLAST-Start: [RecT [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.671, -3.5707972674952195, yes F: RecT-like ssDNA binding protein SIF-BLAST: ,,[RecT [Mycobacterium phage Fishburne] ],,YP_008051271,100.0,0.0 SIF-HHPRED: RecT ; RecT family,,,PF03837.17,57.2727,100.0 SIF-Syn: /note=There was good evidence that this was a RecT-like ssDNA binding protein. The evidence came from HHPRED which had a decent coverage with a high possibility and along with that, there was good evidence on NCBI blast. CDS 33532 - 33813 /gene="48" /product="gp48" /function="hypothetical protein" /locus tag="Venti_48" /note=Original Glimmer call @bp 33532 has strength 13.8; Genemark calls start at 33532 /note=SSC: 33532-33813 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein PBI_FISHBURNE_47 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 1.64837E-61 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.089, -4.741344217713455, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_47 [Mycobacterium phage Fishburne] ],,YP_008051272,100.0,1.64837E-61 SIF-HHPRED: SIF-Syn: /note=Not the longest ORF but all other evidence points to this being the correct start site. CDS 33810 - 34877 /gene="49" /product="gp49" /function="hypothetical protein" /locus tag="Venti_49" /note=Original Glimmer call @bp 33810 has strength 14.81; Genemark calls start at 33810 /note=SSC: 33810-34877 CP: yes SCS: both ST: SS BLAST-Start: [FtsK-like protein [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.02, -2.8793565916978188, yes F: hypothetical protein SIF-BLAST: ,,[FtsK-like protein [Mycobacterium phage Fishburne] ],,YP_008051273,100.0,0.0 SIF-HHPRED: SIF-Syn: /note=There is not enough evidence to call a function. There is low coverage but a high probability on HHRPED however, this is not enough information to call it. Along with that there is little evidence that this has a function on phagesDB CDS 34877 - 35377 /gene="50" /product="gp50" /function="RusA-like resolvase (endonuclease)" /locus tag="Venti_50" /note=Original Glimmer call @bp 34877 has strength 11.42; Genemark calls start at 34877 /note=SSC: 34877-35377 CP: yes SCS: both ST: NI BLAST-Start: [RusA-like protein [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 3.02646E-117 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.006, -4.966271270034708, no F: RusA-like resolvase (endonuclease) SIF-BLAST: ,,[RusA-like protein [Mycobacterium phage Fishburne] ],,YP_008051274,100.0,3.02646E-117 SIF-HHPRED: SIF-Syn: /note=Not the longest ORF but all other evidence points to this being the correct start site CDS 35371 - 35613 /gene="51" /product="gp51" /function="hypothetical protein" /locus tag="Venti_51" /note=Original Glimmer call @bp 35371 has strength 13.84; Genemark calls start at 35371 /note=SSC: 35371-35613 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_FISHBURNE_50 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 5.72895E-52 GAP: -7 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.537, -3.8362837638757656, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_50 [Mycobacterium phage Fishburne] ],,YP_008051275,100.0,5.72895E-52 SIF-HHPRED: SIF-Syn: /note=There is not enough evidence that leads us to think that this has a function to it. There is little coverage that is on HHPRED and the coverage that is high are functions unrelated to ours. Along with that phagesDB agrees that there should not be a function called. CDS 35610 - 35894 /gene="52" /product="gp52" /function="hypothetical protein" /locus tag="Venti_52" /note=Original Glimmer call @bp 35610 has strength 13.2; Genemark calls start at 35610 /note=SSC: 35610-35894 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_FISHBURNE_51 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 1.80455E-59 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.672, -3.6476983004329377, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_51 [Mycobacterium phage Fishburne] ],,YP_008051276,100.0,1.80455E-59 SIF-HHPRED: SIF-Syn: CDS 36036 - 36263 /gene="53" /product="gp53" /function="hypothetical protein" /locus tag="Venti_53" /note=Original Glimmer call @bp 36036 has strength 10.75; Genemark calls start at 36036 /note=SSC: 36036-36263 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein PBI_FISHBURNE_52 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 8.20257E-46 GAP: 141 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.224, -4.4747668481608205, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_52 [Mycobacterium phage Fishburne] ],,YP_008051277,100.0,8.20257E-46 SIF-HHPRED: SIF-Syn: /note=There is very little evidence that a function can be called for this. The coverage on HHPRED was very low and on top of that, there was little probability. Along with that, there is no evidence on phagesDB that there is a function to be called. CDS 36260 - 36583 /gene="54" /product="gp54" /function="hypothetical protein" /locus tag="Venti_54" /note=Original Glimmer call @bp 36260 has strength 14.96; Genemark calls start at 36260 /note=SSC: 36260-36583 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein I5J45_gp53 [Mycobacterium phage Bartholomew] ],,NCBI, q1:s1 100.0% 1.62665E-70 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.202, -2.5969056218148614, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein I5J45_gp53 [Mycobacterium phage Bartholomew] ],,YP_009964778,100.0,1.62665E-70 SIF-HHPRED: SIF-Syn: CDS 36580 - 36819 /gene="55" /product="gp55" /function="hypothetical protein" /locus tag="Venti_55" /note=Original Glimmer call @bp 36580 has strength 14.08; Genemark calls start at 36580 /note=SSC: 36580-36819 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein I5J45_gp54 [Mycobacterium phage Bartholomew] ],,NCBI, q1:s1 100.0% 3.05954E-48 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.838, -5.299065738275371, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein I5J45_gp54 [Mycobacterium phage Bartholomew] ],,YP_009964779,100.0,3.05954E-48 SIF-HHPRED: DUF5076 ; Domain of unknown function (DUF5076),,,PF16826.8,44.3038,45.8 SIF-Syn: /note=Starterator called as the most annotated/suggested start site. CDS 36816 - 37484 /gene="56" /product="gp56" /function="helix-turn-helix DNA binding domain" /locus tag="Venti_56" /note=Original Glimmer call @bp 36816 has strength 15.45; Genemark calls start at 36816 /note=SSC: 36816-37484 CP: yes SCS: both ST: SS BLAST-Start: [helix-turn-helix DNA binding protein [Mycobacterium phage Bartholomew] ],,NCBI, q1:s1 100.0% 4.79894E-158 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.129, -2.7423981586358774, yes F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding protein [Mycobacterium phage Bartholomew] ],,YP_009964780,100.0,4.79894E-158 SIF-HHPRED: PROTEIN (GAMMA DELTA RESOLVASE); PROTEIN-DNA COMPLEX, DOUBLE HELIX, OVERHANGING BASE, DNA BINDING PROTEIN-DNA COMPLEX; 3.0A {Escherichia coli} SCOP: a.4.1.2, c.53.1.1,,,1GDT_A,47.2973,98.4 SIF-Syn: CDS 37477 - 37629 /gene="57" /product="gp57" /function="hypothetical protein" /locus tag="Venti_57" /note=Original Glimmer call @bp 37477 has strength 8.99; Genemark calls start at 37477 /note=SSC: 37477-37629 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein I5J45_gp56 [Mycobacterium phage Bartholomew] ],,NCBI, q1:s1 100.0% 1.27051E-28 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.046, -3.4175091270247986, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein I5J45_gp56 [Mycobacterium phage Bartholomew] ],,YP_009964781,100.0,1.27051E-28 SIF-HHPRED: DUF6579 ; Family of unknown function (DUF6579),,,PF20219.1,16.0,61.8 SIF-Syn: CDS 37622 - 38488 /gene="58" /product="gp58" /function="DNA methyltransferase" /locus tag="Venti_58" /note=Original Glimmer call @bp 37622 has strength 5.86; Genemark calls start at 37721 /note=SSC: 37622-38488 CP: yes SCS: both-gl ST: NA BLAST-Start: [DNA methylase [Mycobacterium phage Flathead] ],,NCBI, q1:s1 100.0% 0.0 GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.944, -3.0478325568523097, yes F: DNA methyltransferase SIF-BLAST: ,,[DNA methylase [Mycobacterium phage Flathead] ],,YP_009957264,100.0,0.0 SIF-HHPRED: Adenine specific DNA methyltransferase (DpnA); M1.HpyAVI, DNA BINDING PROTEIN; 3.0A {Helicobacter pylori (strain ATCC 700392 / 26695)} SCOP: c.66.1.0,,,5HEK_C,81.9444,100.0 SIF-Syn: /note=Starterator did not have as the most annotated/suggested start site. CDS 38535 - 38618 /gene="59" /product="gp59" /function="hypothetical protein" /locus tag="Venti_59" /note= /note=SSC: 38535-38618 CP: yes SCS: neither ST: NA BLAST-Start: [hypothetical protein I5J42_gp66 [Mycobacterium phage GreaseLightnin] ],,NCBI, q1:s22 100.0% 6.82763E-12 GAP: 46 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.381, -6.063367366410245, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein I5J42_gp66 [Mycobacterium phage GreaseLightnin] ],,YP_009964554,56.25,6.82763E-12 SIF-HHPRED: SIF-Syn: /note=Added this gene based on a review of the genome looking for gaps and coding potential. This gene is called by several other P1 phages, but we cannot achieve a 1:1 BLAST match with this ORF. Went with shorter ORF avoid significant overlap with preceding gene CDS 38622 - 39617 /gene="60" /product="gp60" /function="helix-turn-helix DNA binding domain" /locus tag="Venti_60" /note=Original Glimmer call @bp 38622 has strength 9.46; Genemark calls start at 38622 /note=SSC: 38622-39617 CP: yes SCS: both ST: SS BLAST-Start: [helix-turn-helix DNA binding domain protein [Mycobacterium phage Xula] ],,NCBI, q1:s1 100.0% 0.0 GAP: 3 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.046, -2.7653702713535186, yes F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding domain protein [Mycobacterium phage Xula] ],,YP_010104198,92.7711,0.0 SIF-HHPRED: SIF-Syn: /note=Calls and has the most annotated start. CDS 39614 - 40162 /gene="61" /product="gp61" /function="hypothetical protein" /locus tag="Venti_61" /note=Original Glimmer call @bp 39614 has strength 17.35; Genemark calls start at 39614 /note=SSC: 39614-40162 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein I5J45_gp59 [Mycobacterium phage Bartholomew] ],,NCBI, q1:s1 100.0% 1.21605E-129 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.53, -4.694587342996602, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein I5J45_gp59 [Mycobacterium phage Bartholomew] ],,YP_009964784,100.0,1.21605E-129 SIF-HHPRED: SIF-Syn: /note=Does not have the most annotated start site CDS 40159 - 40455 /gene="62" /product="gp62" /function="hypothetical protein" /locus tag="Venti_62" /note=Original Glimmer call @bp 40159 has strength 5.36; Genemark calls start at 40159 /note=SSC: 40159-40455 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein CM06_gp66 [Mycobacterium phage Babsiella] ],,NCBI, q1:s1 100.0% 5.55315E-63 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.376, -2.253377680616507, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein CM06_gp66 [Mycobacterium phage Babsiella] ],,YP_009013083,100.0,5.55315E-63 SIF-HHPRED: SIF-Syn: CDS 40452 - 41354 /gene="63" /product="gp63" /function="helix-turn-helix DNA binding domain" /locus tag="Venti_63" /note=Original Glimmer call @bp 40452 has strength 9.18; Genemark calls start at 40452 /note=SSC: 40452-41354 CP: yes SCS: both ST: SS BLAST-Start: [helix-turn-helix DNA binding protein [Mycobacterium phage Bartholomew] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.554, -5.800928850169731, no F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding protein [Mycobacterium phage Bartholomew] ],,YP_009964786,100.0,0.0 SIF-HHPRED: SIF-Syn: CDS 41394 - 41537 /gene="64" /product="gp64" /function="hypothetical protein" /locus tag="Venti_64" /note=Original Glimmer call @bp 41394 has strength 1.76 /note=SSC: 41394-41537 CP: yes SCS: glimmer ST: NI BLAST-Start: [hypothetical protein I5J45_gp62 [Mycobacterium phage Bartholomew] ],,NCBI, q1:s1 100.0% 4.61395E-25 GAP: 39 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.984, -4.948730469737535, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein I5J45_gp62 [Mycobacterium phage Bartholomew] ],,YP_009964787,100.0,4.61395E-25 SIF-HHPRED: SIF-Syn: CDS 41542 - 41796 /gene="65" /product="gp65" /function="hypothetical protein" /locus tag="Venti_65" /note=Original Glimmer call @bp 41542 has strength 11.98; Genemark calls start at 41542 /note=SSC: 41542-41796 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein I5J45_gp63 [Mycobacterium phage Bartholomew] ],,NCBI, q1:s1 100.0% 4.4411E-53 GAP: 4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.944, -3.0301037898918786, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein I5J45_gp63 [Mycobacterium phage Bartholomew] ],,YP_009964788,100.0,4.4411E-53 SIF-HHPRED: SIF-Syn: CDS 41796 - 41978 /gene="66" /product="gp66" /function="hypothetical protein" /locus tag="Venti_66" /note=Original Glimmer call @bp 41796 has strength 9.75; Genemark calls start at 41796 /note=SSC: 41796-41978 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_FISHBURNE_67 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 1.46912E-31 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.335, -6.15485315075281, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_67 [Mycobacterium phage Fishburne] ],,YP_008051292,100.0,1.46912E-31 SIF-HHPRED: SIF-Syn: CDS 42051 - 42551 /gene="67" /product="gp67" /function="hypothetical protein" /locus tag="Venti_67" /note=Original Glimmer call @bp 42051 has strength 15.96; Genemark calls start at 42051 /note=SSC: 42051-42551 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein I5J38_gp71 [Mycobacterium phage Willsammy] ],,NCBI, q1:s1 100.0% 4.06144E-118 GAP: 72 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.293, -2.338663114094478, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein I5J38_gp71 [Mycobacterium phage Willsammy] ],,YP_009964246,100.0,4.06144E-118 SIF-HHPRED: SIF-Syn: CDS 42620 - 42763 /gene="68" /product="gp68" /function="hypothetical protein" /locus tag="Venti_68" /note=Original Glimmer call @bp 42620 has strength 3.93; Genemark calls start at 42620 /note=SSC: 42620-42763 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein CM06_gp68 [Mycobacterium phage Babsiella] ],,NCBI, q1:s1 100.0% 3.62304E-25 GAP: 68 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.857, -3.2017655760970833, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein CM06_gp68 [Mycobacterium phage Babsiella] ],,YP_009013085,100.0,3.62304E-25 SIF-HHPRED: SIF-Syn: CDS 42760 - 43086 /gene="69" /product="gp69" /function="hypothetical protein" /locus tag="Venti_69" /note=Original Glimmer call @bp 42760 has strength 11.69; Genemark calls start at 42760 /note=SSC: 42760-43086 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein I5J45_gp67 [Mycobacterium phage Bartholomew] ],,NCBI, q1:s1 100.0% 3.28025E-73 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.006, -5.3524728754355015, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein I5J45_gp67 [Mycobacterium phage Bartholomew] ],,YP_009964792,100.0,3.28025E-73 SIF-HHPRED: SIF-Syn: /note=Do not have most annotated start, Starterator not applicable. CDS 43158 - 43469 /gene="70" /product="gp70" /function="hypothetical protein" /locus tag="Venti_70" /note=Original Glimmer call @bp 43158 has strength 16.54; Genemark calls start at 43158 /note=SSC: 43158-43469 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein I5J45_gp68 [Mycobacterium phage Bartholomew] ],,NCBI, q1:s1 100.0% 3.12913E-65 GAP: 71 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.046, -2.7653702713535186, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein I5J45_gp68 [Mycobacterium phage Bartholomew] ],,YP_009964793,100.0,3.12913E-65 SIF-HHPRED: SIF-Syn: /note=Does not have most annotated start, Starterator not applicable. CDS 43466 - 43765 /gene="71" /product="gp71" /function="hypothetical protein" /locus tag="Venti_71" /note=Original Glimmer call @bp 43466 has strength 13.33; Genemark calls start at 43466 /note=SSC: 43466-43765 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein I5J45_gp69 [Mycobacterium phage Bartholomew] ],,NCBI, q1:s1 100.0% 1.66088E-65 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.977, -4.963787326180191, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein I5J45_gp69 [Mycobacterium phage Bartholomew] ],,YP_009964794,100.0,1.66088E-65 SIF-HHPRED: SIF-Syn: CDS 43762 - 43935 /gene="72" /product="gp72" /function="hypothetical protein" /locus tag="Venti_72" /note=Original Glimmer call @bp 43762 has strength 4.26 /note=SSC: 43762-43935 CP: yes SCS: glimmer ST: NA BLAST-Start: [hypothetical protein I5H92_gp077 [Mycobacterium phage StAnnes] ],,NCBI, q1:s1 100.0% 2.39536E-33 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.107, -2.7863799983944713, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein I5H92_gp077 [Mycobacterium phage StAnnes] ],,YP_009962737,100.0,2.39536E-33 SIF-HHPRED: SIF-Syn: /note=Does not have most annotated start, Starterator not applicable. CDS 43932 - 44048 /gene="73" /product="gp73" /function="hypothetical protein" /locus tag="Venti_73" /note=Original Glimmer call @bp 43932 has strength 13.83 /note=SSC: 43932-44048 CP: yes SCS: glimmer ST: SS BLAST-Start: [hypothetical protein I5J41_gp71 [Mycobacterium phage Jung] ],,NCBI, q1:s1 100.0% 8.73572E-19 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.944, -3.3188993291388478, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein I5J41_gp71 [Mycobacterium phage Jung] ],,YP_009964482,56.7164,8.73572E-19 SIF-HHPRED: SIF-Syn: CDS 44118 - 44489 /gene="74" /product="gp74" /function="hypothetical protein" /locus tag="Venti_74" /note=Genemark calls start at 44118 /note=SSC: 44118-44489 CP: yes SCS: genemark ST: SS BLAST-Start: [hypothetical protein CM06_gp69 [Mycobacterium phage Babsiella] ],,NCBI, q1:s10 100.0% 1.8574E-87 GAP: 69 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.223, -6.903978873041489, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein CM06_gp69 [Mycobacterium phage Babsiella] ],,YP_009013086,93.1818,1.8574E-87 SIF-HHPRED: SIF-Syn: CDS 44578 - 44751 /gene="75" /product="gp75" /function="ribbon-helix-helix DNA binding domain" /locus tag="Venti_75" /note=Original Glimmer call @bp 44578 has strength 9.12; Genemark calls start at 44578 /note=SSC: 44578-44751 CP: yes SCS: both ST: SS BLAST-Start: [gp72 [Mycobacterium virus Fruitloop] ],,NCBI, q1:s11 100.0% 7.73531E-31 GAP: 88 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.258, -4.4057176022952405, no F: ribbon-helix-helix DNA binding domain SIF-BLAST: ,,[gp72 [Mycobacterium virus Fruitloop] ],,YP_002241757,85.0746,7.73531E-31 SIF-HHPRED: SIF-Syn: CDS 44950 - 45288 /gene="76" /product="gp76" /function="hypothetical protein" /locus tag="Venti_76" /note=Original Glimmer call @bp 44950 has strength 14.72; Genemark calls start at 44950 /note=SSC: 44950-45288 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein I5J45_gp74 [Mycobacterium phage Bartholomew] ],,NCBI, q1:s1 100.0% 1.12994E-73 GAP: 198 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.376, -2.1924212546750814, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein I5J45_gp74 [Mycobacterium phage Bartholomew] ],,YP_009964799,100.0,1.12994E-73 SIF-HHPRED: SIF-Syn: CDS 45285 - 45653 /gene="77" /product="gp77" /function="hypothetical protein" /locus tag="Venti_77" /note=Original Glimmer call @bp 45285 has strength 13.75; Genemark calls start at 45285 /note=SSC: 45285-45653 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_FISHBURNE_75 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 1.38918E-84 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.745, -3.361207249949735, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_75 [Mycobacterium phage Fishburne] ],,YP_008051300,100.0,1.38918E-84 SIF-HHPRED: SIF-Syn: CDS 45640 - 45888 /gene="78" /product="gp78" /function="hypothetical protein" /locus tag="Venti_78" /note=Original Glimmer call @bp 45640 has strength 13.92; Genemark calls start at 45640 /note=SSC: 45640-45888 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_FISHBURNE_76 [Mycobacterium phage Fishburne] ],,NCBI, q1:s1 100.0% 4.37282E-51 GAP: -14 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.659, -6.039334287572722, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_FISHBURNE_76 [Mycobacterium phage Fishburne] ],,YP_008051301,100.0,4.37282E-51 SIF-HHPRED: SIF-Syn: CDS 45893 - 46276 /gene="79" /product="gp79" /function="HNH endonuclease" /locus tag="Venti_79" /note=Original Glimmer call @bp 45893 has strength 4.44; Genemark calls start at 45893 /note=SSC: 45893-46276 CP: yes SCS: both ST: SS BLAST-Start: [HNH endonuclease [Mycobacterium phage Phineas] ],,NCBI, q1:s80 100.0% 2.32542E-87 GAP: 4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.001, -5.3628583592769, no F: HNH endonuclease SIF-BLAST: ,,[HNH endonuclease [Mycobacterium phage Phineas] ],,YP_009964725,61.6505,2.32542E-87 SIF-HHPRED: SIF-Syn: