LOCUS CabbageMan 15612 bp DNA linear 30-AUG-2024 DEFINITION Arthrobacter phage CabbageMan. ACCESSION VERSION KEYWORDS . SOURCE Arthrobacter phage CabbageMan ORGANISM Arthrobacter phage CabbageMan Viruses;dsDNA viruses, no RNA stage;Caudovirales;Podoviridae. REFERENCE 1 (bases 1 to 15612) AUTHORS Alford,J.R., Dayton,A.M., Droin,L.M., Bawab,T.F., Gojan,E.S., Fox,B.D., Aiyana,O., Abuelsamen,N.R., Araji,R., Hernandez-Morrison,M., Nyiligira1,C., Valera,L.C., Lypaczewski,P.C., Klyczek,K., Viland,M.D., Lewis,C.M., Garlena,R.A., Russell,D.A., Jacobs-Sera,D. and Hatfull,G.F. TITLE Direct Submission JOURNAL Submitted (30-AUG-2024) Biology, University of Wisconsin-River Falls, 410 South Third Street, River Falls, WI 54016, USA COMMENT Phage isolation, DNA extraction, and annotation analysis performed at McGill University, Montreal, Canada Sequencing by Illumina to approximately 4281x coverage performed at the Pittsburgh Bacteriophage Institute, PIttsburgh, PA Assembly performed using Newbler and Consed as of January, 2024 Supported by Science Education Alliance, Howard Hughes Medical Institute, Chevy Chase, MD This genome has defined ends with 15-base 3' overhangs (CCACGTATACCGTCC). FEATURES Location/Qualifiers source 1..15612 /organism="Arthrobacter phage CabbageMan" /mol_type="genomic DNA" /isolation_source="soil, Montreal, Canada" /lab_host="Arthrobacter globiformis B-2979" /country="Canada" /lat_lon="45.51499 N 73.81362 W" /collection_date="01-Oct-2023" /collected_by="Ximena Iraheta" /identified_by="Ximena Iraheta" /note="complete genome" gene 258..440 /gene="1" /locus_tag="SEA_CABBAGEMAN_1" CDS 258..440 /gene="1" /locus_tag="SEA_CABBAGEMAN_1" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MKHGSLAPWPECLCGGPSWQECQGGLAATARPAGPPDQLDPGTL VSRARIDYLFWRGFDT" gene 437..1996 /gene="2" /locus_tag="SEA_CABBAGEMAN_2" CDS 437..1996 /gene="2" /locus_tag="SEA_CABBAGEMAN_2" /codon_start=1 /transl_table=11 /product="terminase" /translation="MSNTTLEAPAFHDLDGWPLDLGSIDWDGAYQRMVGSWGEKRGVV YAAVPSALSPVPDGTDIAAAVLGSRMLGMELTPQGCQVAAVLEARNPFGLPLYDDVTV EVPRRATKTTSIQAVLLGRCETRPGYRIIQTAQDGTRASMVFMDMVRTLERVTPDEAD RTWKVFKSTGREYLEWENGSRWWVGPPKPSTYRGLAADVLWFDESGELSPEESDDLEA GALPVMDTRDDGQVIKSGTPGLVRAGLFWKSLAAARATPDQLGVVAYGARESEVVDLD EDGAMALLWRCHPGLACGLTTEAKMRKRFQVMDLAQFIREYLCVWPPDTTKTALDMVK FAAGEVNPVPLPTGVPFAFGYNVAQGGVAAAVAVAWYDGDQLRGQVMEYRVKADWLAD DLGRILKAHAGVPIGYDAIGDNLAKAQEVARRPRTRAAALKALTLKEVAAGTAALAMA VDAGTFRWGTSPSLAKAAGAASWRDSGGSRLFRRIEGQDISSLLALVHAVAAVSTMKK RRSGGGGYAAQ" gene 2076..2330 /gene="3" /locus_tag="SEA_CABBAGEMAN_3" CDS 2076..2330 /gene="3" /locus_tag="SEA_CABBAGEMAN_3" /codon_start=1 /transl_table=11 /product="helix-turn-helix DNA binding domain" /translation="MTTKKKTYETADYAAMMRRMMKAHGRRVADGDVEDLAELMELQA YLDYVVRGAVQAQREVQGKSWADIARAAGTSRQAAQKKYS" gene 2437..3570 /gene="4" /locus_tag="SEA_CABBAGEMAN_4" CDS 2437..3570 /gene="4" /locus_tag="SEA_CABBAGEMAN_4" /codon_start=1 /transl_table=11 /product="portal protein" /translation="MPRLFDTARTIMGWTAGTLNTGASSLTGVTWATSTALSPILPPS LPRASVSVADALRCPPVARAVGLYSAAFLKSEWRASDPTLAWLNKSSGPVSPAVRNVR TLVDLFMANRALWITSGTGTDRQEAVHLYAHLWSVDEVGVVRLGGVGPDANGDYVVGG KVITNTANLIYFPGAMPLGFLDAAEDSLTYYKDLAATIKSRGKTPMPLMELKMLDAYD GPLPGDADYDEATDLILNTQKSYAAARNSPDGAVTVTPQGVDLIVHQPDDDGGMLIEA RNAVRVDVANHANINASMLDGNTGGSDDYANTLQKRNEFEDLSLALFTAPILARLSLD DVTPPGELVELVPFDSGADASGNTGDAVGIPQTRAAGLLTGNA" gene 3627..5267 /gene="5" /locus_tag="SEA_CABBAGEMAN_5" CDS 3627..5267 /gene="5" /locus_tag="SEA_CABBAGEMAN_5" /codon_start=1 /transl_table=11 /product="minor capsid and protease fusion protein" /translation="MKHVLGELVLASAQDRTLKYRVLPFGEQSAVMSSGHQFSVEPGA VELPTDPSTVHVNEEHDRTRPIGRFTSLEEAPDGIYGTVTVADTTAGNDALALASAGL RKGISVEIGNLVIRGGKLLKGTLEAAALVVAPSFGSALALGSALPPAEDQGDLSDDLA AAAAAIAAAQERLAPPAQENTPDGGADSPPSGDTTATASTSTSTTKEGTMFKTAQAAQ EAADAVATAAATATAAKAVNPLLALGSALQAASSPLSGQVAKAAALDTIVQADVFDAV TVPDYVGELWKGRRYQGRYLDLVSDAPMVSQSVVGWRWIEGKTPQVARWTAPSSGGTM NDIPTNEVKAESKTYNGFRIAGGHQVDRIHVDLPNAEWWASYNLESADDYARKLDAEI IAHVTDPANFSTVTLDAGATYEDQLLEGIGAAIEFADPDHILVGWNVFKFLALQKMLD QKAVHPGEFAQANGAFKVSYLDIPLKVAPRSLTAVAEKVQVGSRYANVLHSLPGGPIR VDAEEIQKATVQHGVFGYHILRPTDTRGSINVAVAPAG" gene 5272..5613 /gene="6" /locus_tag="SEA_CABBAGEMAN_6" CDS 5272..5613 /gene="6" /locus_tag="SEA_CABBAGEMAN_6" /codon_start=1 /transl_table=11 /product="head-to-tail adaptor" /translation="MAGLESTIGWLSVQEVIDLWPDAPGEEENAYAELANLMGTAHEV LETKGPPLDPPPERYKTAQLLYMKHLWARQRAGNGDQLGVDGLAVSTWPLVQEAFGLM RLNRRGVRGLR" gene 5610..6035 /gene="7" /locus_tag="SEA_CABBAGEMAN_7" CDS 5610..6035 /gene="7" /locus_tag="SEA_CABBAGEMAN_7" /codon_start=1 /transl_table=11 /product="tail terminator" /translation="MSDTDNGLEALSAAEQLRDKFQAALTNWTWKAAPEPPGNVTRKR YGVIWRTGLGKGSRDGRLVTKMQINIYAARTAGEALERELDDALDDVLLELQRISDVR FQEATRVTFAETFPGWEVLLEIEHNNYYAAAVRQEAGTA" gene 6035..6448 /gene="8" /locus_tag="SEA_CABBAGEMAN_8" CDS 6035..6448 /gene="8" /locus_tag="SEA_CABBAGEMAN_8" /codon_start=1 /transl_table=11 /product="major tail protein" /translation="MAHTQLEIKNATVKAAVGAGTLADFADAIDNVKLQTAYTTTRWN GVSGKSAVTATPLKDTIVVNVGDSLKTGEFWAMLRANHGLAGKVEFCPLGGTSPKIAA DVLLVAPGALGGAEGGNSSTVTLECLAPATITYAP" gene 6453..6983 /gene="9" /locus_tag="SEA_CABBAGEMAN_9" CDS 6453..6983 /gene="9" /locus_tag="SEA_CABBAGEMAN_9" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MGSILTPSAKSVDELRAAAIGLRLLPRNIRNDLNKNRRAVLNPM WKEAVNARAVTTLDKLVLAKGARVAPGNPATAMAATSKRPLSGGLVPDNRDQAVAIEF GTPERQAVETYTRKSRKSGKQHKVTRHTKRQLPVIQRKGRVIYAAWRDIAPRIISLDA QTIARRIYEAFEGKAQ" gene 6980..8647 /gene="10" /locus_tag="SEA_CABBAGEMAN_10" CDS 6980..8647 /gene="10" /locus_tag="SEA_CABBAGEMAN_10" /codon_start=1 /transl_table=11 /product="tape measure protein" /translation="MTMKFTFASDVDGVVRGTKDIADRFDEVSDTLVDLARRGEDSGD QLADSLGDSGQAADKLEAKLKDALDAAQDLAKGEKAADALGDGMKDAGKDVERFEDKA KGAFKSLGDSARKSGDVVGSAMKQGTKEAEGGLEDFRDEANSTAKETAASFDGSFDSI AGMAQEVAANAFAGFGPAGAVAGLAAAAGIGIAITQMQAGAEAANKMNEAAVDLAGQI VEAGGDIRDVDLGAIVSAWGREVIEDNWITPWANEASTKFQETAKDAQDANRDVAESV RAAAGSADDSRKFLDDTAETWQRLTRRIQEGTTTTMDGLPVQDEAARAAQKERDALSD LRGQAEENIKTKEAAVDIAEIETAATGDNTAAIEANIQALEDKAAAMADAAGVAMDAT TAEIQYADTMAQGAKDIAANGKGLELNTATGRANQQTLVDMAKSANELRDAQVAAGGS TADITAKQNQARTAFINAAVAAGMGKTEAGKLATQYGLIPGNVDTYVKAHNVQKTKDE IDGVAEPRTVPIHLTRGSESISSWIQGLSGRTIPVNMAVRGGRAVDD" gene 8753..10153 /gene="11" /locus_tag="SEA_CABBAGEMAN_11" CDS 8753..10153 /gene="11" /locus_tag="SEA_CABBAGEMAN_11" /codon_start=1 /transl_table=11 /product="minor tail protein" /translation="MAAISAELLPQSAAVRLAINAPAGIRSITRSDANGVNEVRPGPG VLDVQPPLVDDATNLVPNPSFQVNTTGWTASRSVVTSYTFAGSFLNYPGQLAGTKGVK VVPDGTVGTVTTSAGDFTVAPGAWVAVAGNVSASAGNARLGIAFYNGSTWLSETYSDP IPGGTSVYKLTRLSVAAQAPANTTKYRLLVRLYAADGSAAPGASCTVYGDKFSATPDA GSEAAALAQVAFYHDGSTSDSPMEFETSWSGTAHNSASTRARPVTPFYVTDYEAASGP IRYDVVDGNGLFETLDVNGFVLDSPWLFTPIIPGYSRQLRTVRGIGADLENLSTVHAG LLGRTDPIVVMRPVGLRSGTMTIWAGTYAAALDIVSPLKQATPMMLRQPDHAGLDMYF APLGAALVHEDQEGAGTTWGVQVRYQEIKRPEGDIAGALGWTYADLEAAVPRYSDLPR TFATYADMRLNKRITP" gene 10150..11286 /gene="12" /locus_tag="SEA_CABBAGEMAN_12" CDS 10150..11286 /gene="12" /locus_tag="SEA_CABBAGEMAN_12" /codon_start=1 /transl_table=11 /product="minor tail protein" /translation="MTATTARAGRYQDRALDIVKQSHRQDAAGQLEVLGAAPETLAVV LRDPVVTFAEDWAPYVQVSADGVAPVDVAELAKIDPRQAVQVEVKAGYVYDDGEADVQ RLALVHLRSRRAVLPDGNMPLVGESAERLAQDAKWLPATITKTFPGVLEAMQFMVNYA QGHTDAVWITTIEPGWRPDLVTSVVLEQGTELWEPLAALALSAGLRLWADEDGAWNLA PKTTLAGVTAAFLKQGPNTTVKKVEDVLSREGWYNAAALTYQWKDAQGVEKTVVGTWS PAAPAGLDKGAGCRTYAADRPGPVSQWQANENARLTVANLSSRGWSYVVDSVAMYWLR PGMTVQVDLANGTTARHIVRRVVFQVAEGTMTVTTREPSNTEES" gene 11291..11695 /gene="13" /locus_tag="SEA_CABBAGEMAN_13" CDS 11291..11695 /gene="13" /locus_tag="SEA_CABBAGEMAN_13" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MPVTTNRAYRYPGAGVEPDVNGDIQRLGEDVDADMAATLAALAK LPGRIQTGSATISIGSAVASATQVITFPVAFTSTPNVFLQSTANVAGRASLLSLYVNA TSATQFTTKIQTADNANTGTSYAIAYNWIAVL" gene 11695..12432 /gene="14" /locus_tag="SEA_CABBAGEMAN_14" CDS 11695..12432 /gene="14" /locus_tag="SEA_CABBAGEMAN_14" /codon_start=1 /transl_table=11 /product="endolysin" /translation="MEFYLPHNGPRTQVFGNIQPDGHPHAGDDFGYTGADGQVHPEVY AAAPGVVLYAGDSRALGWPNPYFLNPDFDRTDARDDSAGNAVIIRHDDGGMTTYNHLE SWSVRRGQRVTGRQRIATTGSTGRSTGKHLHFEWIPYPCNFNTPTYGRVRPTFKPRGI FMYLTQKQELEVLTAARAINLRAKYLDAPVSAVPRKSAAEYLDTAIPRKGGRTGNTTP RNTIAYQDANLDAVKAAAAARDQAVAE" gene 12429..12629 /gene="15" /locus_tag="SEA_CABBAGEMAN_15" CDS 12429..12629 /gene="15" /locus_tag="SEA_CABBAGEMAN_15" /codon_start=1 /transl_table=11 /product="membrane protein" /translation="MTAPAPRNVGPVTTGASAGYAGSVVLVYVIEQLTARDVPDPVEL ALGVLLTVGGGFLVRSRAVAGG" gene 12622..12864 /gene="16" /locus_tag="SEA_CABBAGEMAN_16" CDS 12622..12864 /gene="16" /locus_tag="SEA_CABBAGEMAN_16" /codon_start=1 /transl_table=11 /product="membrane protein" /translation="MAEAPVQDVTAWRLGKIEIALDAVLVKLDDRPDWKDVRNIEDQL AARITRLEDWQTWAIRIVVGAVLLAVVGLALAVGRT" gene 13096..13374 /gene="17" /locus_tag="SEA_CABBAGEMAN_17" CDS 13096..13374 /gene="17" /locus_tag="SEA_CABBAGEMAN_17" /codon_start=1 /transl_table=11 /product="helix-turn-helix DNA binding domain" /translation="MQMPGRYRHPDDTARVVTPSDLIGAQEACALLGIDRSTLTRWIQ SGATLPLAQLDGPGGAYVFDRTDVEAQARNIQAATAAMPLVKKGPQKE" gene 13371..13559 /gene="18" /locus_tag="SEA_CABBAGEMAN_18" CDS 13371..13559 /gene="18" /locus_tag="SEA_CABBAGEMAN_18" /codon_start=1 /transl_table=11 /product="helix-turn-helix DNA binding domain" /translation="MRARPVFVSITDAANILGVSARTVKRYVADGHLDAGELPHGQVR LARAQVIGLVRTTPRNPE" gene 13556..14431 /gene="19" /locus_tag="SEA_CABBAGEMAN_19" CDS 13556..14431 /gene="19" /locus_tag="SEA_CABBAGEMAN_19" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MNQPGKTFTVRRGQTAMPNRTIYDSYTSLGGGGEDARQLSIQAL GVLLLGLSRPDTAAQGYRAFLGRGLGRDGLLKALKELNLTGHRHQFKRNGPGGHMVTD TLLSEVPMTPDAASEEWKALVLAMDADARQAAKDGAAAYARQAEAMARELAEQDAAEA AAACQRLADQLAAEAAGMPGRDRAPENGASGTVEGSAPCDGSPAYGEAAHLSVPDSQV SKETSSFRDRDNQASEKSEDQERPAQGGAAVSNDAATGPGHAAVLDILAERRSKMRAL KTKSLRSGHGQEAGA" gene 14431..14682 /gene="20" /locus_tag="SEA_CABBAGEMAN_20" CDS 14431..14682 /gene="20" /locus_tag="SEA_CABBAGEMAN_20" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MSRATDKTLRAAAFKEAAFTARMAEESRKRARAAWKDAQARGAG QDELRALEVQTRIAYARATTAADLEDEAWQHYAATLPHG" gene 14675..15058 /gene="21" /locus_tag="SEA_CABBAGEMAN_21" CDS 14675..15058 /gene="21" /locus_tag="SEA_CABBAGEMAN_21" /codon_start=1 /transl_table=11 /product="HNH endonuclease" /translation="MARRPAPPQGQLFRMDPVQVPGQDGHRAKKWGGRKVAKARDTVR AMLPAACWRGCGRMITRDWPEKDWHAGHVNGRAEGGPDNVTNYLPECRWCNLSEGGKV GAAITNGKRVAITTDAIRERTLKWY" gene 15049..15219 /gene="22" /locus_tag="SEA_CABBAGEMAN_22" CDS 15049..15219 /gene="22" /locus_tag="SEA_CABBAGEMAN_22" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MVLENETGEPGTDLFRCDSCPQEIEGRTWTWLERQVSNHTRANK GHRMFMVLDGEA" gene 15216..15362 /gene="23" /locus_tag="SEA_CABBAGEMAN_23" CDS 15216..15362 /gene="23" /locus_tag="SEA_CABBAGEMAN_23" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MSRKGGGQRRHAMDPVTLVHMARLEPAAAQYILDRRRRGVPARG LGRP" gene 15359..15574 /gene="24" /locus_tag="SEA_CABBAGEMAN_24" CDS 15359..15574 /gene="24" /locus_tag="SEA_CABBAGEMAN_24" /codon_start=1 /transl_table=11 /product="membrane protein" /translation="MNPGLDQDLAVLALLLLALVLVAAGVGLWWDSCRRRGHEDLELT ALCNDGRVAWRARRCQRCGSLRFEELV" ORIGIN 1 gcttccattg cgcgcgtggg ggccatggtg aaggtggacc gccagctgct tcccgccatg 61 caacgcgagg acgaagcggc caggaaattc ctggcgaaaa tgcgcccgga aaatgccctg 121 gaaaatcggc cggaattttt gccggcgtcg gtcctgattc ccgtcacctg gacgtgcgcc 181 agggacgggg tttttagatc ccgggcgccg ctgtccaagc tggacgagtg gctggcccag 241 ggctggcgga tagcggattg aaacacggca gcctggcccc atggccggaa tgcctatgcg 301 gcgggccgtc ctggcaggaa tgccagggcg gcctggccgc gacagctagg ccagctggtc 361 cgccggacca gctggacccg ggaacgctgg tgtcccgggc acggattgac tacctgtttt 421 ggagagggtt cgacacatga gtaacaccac cctggaggcc ccggccttcc atgacctgga 481 cggctggccg ctggacctgg gcagcattga ttgggacggc gcctaccagc gcatggtggg 541 gtcctggggc gagaagcgcg gcgtggtcta cgccgccgta ccgtccgccc tgtcgcccgt 601 cccggacggc acggacatag cggcggcggt cctggggtcc cggatgctgg gcatggaact 661 gacgccccag ggctgccagg tggccgccgt cctggaggcc cggaacccgt tcgggctgcc 721 gctgtacgac gacgtgacgg tggaggttcc caggcgggcg actaagacca cgtccattca 781 ggcggtgctg ctgggccggt gcgagacacg cccgggctac cgcatcatcc agacggccca 841 ggacggcacc cgggcgtcca tggtgtttat ggacatggtg cggaccctgg agcgcgtgac 901 gccggacgaa gcggaccgga cctggaaggt gtttaagtcc accgggcgcg agtacctgga 961 gtgggagaac ggaagccgct ggtgggtagg tccgccgaag ccgtccacct accggggcct 1021 ggcggcggac gtgctgtggt ttgacgagtc cggcgaactg tcccccgagg aatcggacga 1081 cctggaggcg ggcgccctgc cggtcatgga taccagggat gacggccagg tgatcaagag 1141 cggcaccccg ggcctggtcc gggcgggcct gttctggaag tccctggccg ccgcccgggc 1201 cacgccggac cagctgggcg tggtggcata cggggccagg gaatccgaag tggtggacct 1261 ggacgaggac ggcgccatgg ccctgttgtg gcggtgccac ccgggcctgg cgtgtggact 1321 gacgacggaa gcgaagatgc gcaagcggtt ccaggtcatg gacctggccc agttcatccg 1381 ggaatacctg tgcgtgtggc cgccggacac cacgaaaacg gccctggata tggtcaagtt 1441 tgccgccggg gaagtgaacc cggtcccgct gccgacgggc gttccgttcg cctttggcta 1501 caacgtggcc cagggcggcg tggctgccgc cgtggccgtc gcctggtacg acggggacca 1561 gctgcgcggc caggtcatgg aataccgcgt gaaggcggac tggctggccg acgacctggg 1621 ccggattctg aaggcccacg ccggtgtccc catcggctac gacgcgatag gcgacaacct 1681 ggcgaaggcc caggaagtcg cccggcgccc caggacccgg gcggcggcgt tgaaggccct 1741 gacgctaaag gaagtcgcgg cgggcaccgc tgccctggcc atggccgtgg acgcggggac 1801 cttccggtgg ggcacgtccc cgtccctggc gaaggctgcc ggggcggcgt cctggcgcga 1861 cagcggcggg tcccggctgt tccgccggat tgagggccag gacattagtt cgctgctggc 1921 cctggtccat gccgtggcgg cggtgtccac catgaagaaa cgccgaagcg gcggcggcgg 1981 atacgccgcc cagtagggcc tggaacggcc cgccccgggc agctggggcg ggccggtgct 2041 tgtgtccaat tcactgtcaa catattgttg acagcatgac gacgaagaaa aagacctacg 2101 aaacagcgga ctacgccgcc atgatgcgcc gcatgatgaa ggcccacgga cgccgcgtgg 2161 cggacgggga cgtggaggac ctggccgaac ttatggaact ccaggcgtac ctggactacg 2221 tggtgcgcgg cgccgtccag gcccagcggg aagtccaggg caagtcctgg gcggacattg 2281 cccgggccgc tggcacgtca cgccaggccg cccagaagaa atacagctga acccaaaccg 2341 gccatagctg gacaccgctg gacagccgga aagtagtacc ccgggcgggg ccaaaacagg 2401 cccggaacgg ccccgcccgg gcctagtgtt ctgtccgtgc cgagactatt tgataccgcc 2461 cgaaccatca tgggatggac cgccggaacc ctgaacaccg gggccagttc ccttacgggc 2521 gtgacctggg cgacgagcac ggcactatcc cccatcctgc cgccgtcgct gccccgggcg 2581 tcggtgtccg tggcggacgc cctgcggtgt cccccggtgg cccgggccgt ggggctgtac 2641 tccgctgcgt tcctgaaatc ggaatggcgg gcatcggacc ccaccctggc gtggctgaac 2701 aaatcgtccg gcccggtgtc cccggcggtc cgcaacgtgc ggacgctggt ggacctgttc 2761 atggcgaacc gtgccctgtg gatcaccagc ggcaccggca cggaccgcca ggaggccgtt 2821 cacctgtacg cccatctgtg gagcgtggac gaagtgggcg tggtccgcct gggcggcgtc 2881 gggccggacg ctaacgggga ctacgtggtg ggcgggaagg tcatcaccaa cacggcgaac 2941 ctgatctatt tcccgggcgc catgcccctg gggttcctgg acgccgccga agattccctg 3001 acgtactaca aagacctggc cgccaccatc aagtcccggg gcaagactcc catgcccctt 3061 atggaactga agatgcttga cgcctatgac ggaccgctgc ccggtgacgc ggactacgac 3121 gaagccacgg acctgattct gaacacgcag aagtcctacg ctgcggcccg caattccccg 3181 gacggcgccg tgaccgtgac gccccagggc gtggacctga tagtgcacca gccggacgac 3241 gacggcggca tgctcattga ggcccggaac gcggtccgcg tggacgtggc caaccatgcg 3301 aacatcaacg cttccatgct ggacgggaac accggcgggt ccgacgacta cgccaacacg 3361 ctgcaaaagc gcaacgagtt tgaggacctg tccctggccc tgttcaccgc cccgattctg 3421 gcccggctgt ccctggacga cgtgacgccg cccggggaac tggtggagct cgtgcccttt 3481 gacagcggcg ccgacgcatc cgggaacacc ggcgacgctg taggaatccc acaaactcga 3541 gccgccggcc ttttgaccgg gaacgcctag aacacgcgga acccgtaaat cacccgttgt 3601 acgaacccaa caaaagacag gaacccatga aacacgttct aggggaactg gtgctggcat 3661 cggcccagga ccgcaccctg aaatatcgcg tcctcccgtt cggggaacag tccgccgtga 3721 tgtccagcgg ccaccagttc agcgtggaac ccggcgccgt ggaactcccc acggacccgt 3781 ccaccgtcca cgtcaacgag gaacacgaca ggacccggcc catcgggcgc ttcacgtccc 3841 tggaggaagc cccggacggc atctacggca ccgtgaccgt ggcggacacc accgccggga 3901 acgacgccct ggcgctggcg tccgccgggc tgcggaaggg catcagcgtt gaaatcggga 3961 acctggtaat ccggggcggc aagctgctga aaggcaccct ggaggccgcc gccctggtag 4021 tcgccccgtc ctttgggtcc gccctggccc tggggtccgc cctgccccca gctgaggacc 4081 agggcgacct gtccgacgac ctggccgccg ccgccgccgc tatcgctgcg gcccaggaac 4141 gcctggcgcc accggcccag gagaacaccc cggacggcgg cgccgattcc ccgccgtccg 4201 gggacaccac cgccaccgca tccaccagca cgtccaccac gaaagaagga accatgttca 4261 aaaccgccca ggctgcccag gaggccgccg acgccgtcgc cacggctgcc gccacggcga 4321 ccgctgcgaa ggccgtaaac ccgctgctgg ccctggggtc cgcactccag gccgcgtcgt 4381 ccccgctgtc cggccaggtg gcgaaggccg ccgccctgga caccatcgtt caggccgacg 4441 tgttcgacgc cgtcaccgtc ccggactacg tgggcgaact gtggaagggc cgccgctacc 4501 agggccgcta cctggacctg gtgagcgacg cgcccatggt gtcccagtcc gtggtgggct 4561 ggcgctggat tgagggcaaa acgccccagg tggcccgctg gaccgccccg tcgtccggcg 4621 gcaccatgaa cgacattccc accaacgagg tgaaagcgga atccaagacc tacaacgggt 4681 tccgtatcgc gggcggccac caggttgacc gcatccacgt tgaccttccg aacgccgaat 4741 ggtgggcgtc ctacaacctt gagtccgccg acgactacgc ccggaagctg gacgcggaaa 4801 tcatcgccca cgtcacggac ccggcgaact tttccaccgt caccctggac gccggggcga 4861 cctacgaaga ccagctgctg gaaggcatcg gcgccgctat cgagttcgcg gacccggacc 4921 acatcctggt gggctggaac gtgtttaagt tcctggcgct ccagaagatg ctggaccaga 4981 aggccgtcca cccgggcgag ttcgcccagg cgaacggcgc gttcaaagtg tcctacctgg 5041 acatcccgct gaaggtggcc ccgcgctccc ttacggcggt ggccgaaaag gtccaggtgg 5101 ggtccaggta cgccaacgtg ctgcactccc tgcccggcgg ccccatccgc gtggacgccg 5161 aagaaatcca gaaggcgacc gtccagcatg gtgtgttcgg ctaccacatc ctgcggccca 5221 cggacacgcg cggttcgatc aacgtcgctg tagctccggc gggctaggac ggtggcgggc 5281 ctggaatcca ccatcggctg gctgtctgtc caggaggtca tcgacctgtg gccggacgcc 5341 ccgggcgagg aagaaaacgc ctacgccgaa ctggccaacc ttatgggcac cgcccacgag 5401 gtcctggaga caaaaggccc gccgctggac ccgcccccgg aacgctacaa aacggcccag 5461 ctgctctaca tgaagcatct gtgggcgcgg cagcgggccg ggaacgggga ccagctgggc 5521 gtggacggcc tggcggtgtc cacctggccg ctggtccagg aagcattcgg acttatgcgc 5581 ctgaaccggc gcggcgtaag gggccttaga tgagtgacac ggacaacggc ctggaggccc 5641 tgtcagcggc ggaacagctg cgcgacaagt tccaggccgc cctgacgaac tggacctgga 5701 aggcggcccc tgaaccgccc gggaacgtga ccaggaagcg ctacggcgtg atctggcgga 5761 cgggcctggg caaagggtcc agggacgggc gcctggtaac gaagatgcaa atcaacatct 5821 acgccgccag gaccgccggg gaagccctgg aacgggaact ggacgacgcc ctggacgacg 5881 tgctgctgga gctacaacgc atcagtgacg tgaggttcca ggaagccacc agggtgacgt 5941 ttgccgagac gttcccgggc tgggaagtcc tgctggaaat tgagcacaac aactactatg 6001 ccgccgccgt gcggcaggaa gcggggacgg cctaatggca catacccaac tggaaatcaa 6061 gaatgccacc gtgaaggctg cggtgggcgc cgggaccctg gcggactttg ccgacgcaat 6121 cgacaacgtc aagcttcaga cggcctacac cacgacgcgc tggaacggcg tgtcgggcaa 6181 gtccgccgtg acggccacgc cgctgaagga caccatcgtg gtgaacgtgg gcgactccct 6241 gaagacgggc gagttctggg ccatgctgcg ggcgaaccac ggcctggccg ggaaggtgga 6301 gttctgcccg ctgggcggca cgtccccgaa gattgcggcg gacgtgctgc tggtggcccc 6361 cggcgccctg ggcggtgctg aaggcggcaa cagctccacc gtgacgctgg agtgcctggc 6421 cccggccacg atcacctacg ccccgtagtt ccgtgggcag catccttacc ccgtcggcta 6481 agtccgtgga cgagttgcgg gccgccgcca tcgggctaag gctgctgccc cggaacatcc 6541 gtaacgacct gaacaaaaac cgccgggccg tcctgaaccc catgtggaaa gaggcggtca 6601 acgcccgggc cgtcaccacc ctggacaaac tggtgctagc caagggcgcc agggtggccc 6661 cggggaaccc ggccaccgcc atggccgcca cgtccaaacg gcccctgtcc ggcggcctgg 6721 tcccggacaa cagggaccag gcggtggcta tcgagttcgg caccccggaa cgccaggcgg 6781 tggagacgta cacccggaag tccaggaagt ccgggaagca acacaaagtc acccgccaca 6841 ccaaacgcca gctgcccgtt atccagcgga agggccgcgt gatctatgcg gcctggcggg 6901 acattgcccc gcgcatcatc tccctggacg cccagaccat cgcccgccgc atctacgaag 6961 cattcgaagg aaaagcacaa tgaccatgaa attcacgttc gcgtccgacg tggacggcgt 7021 ggtgcgcggc actaaggaca ttgccgacag gttcgacgaa gtatcggaca ccctggtgga 7081 cctggcccgc cggggcgagg attccgggga ccagctggcg gactccctgg gcgattccgg 7141 ccaggcggcg gacaagctgg aagcgaagct gaaagacgcc ctggacgccg cccaggacct 7201 ggcgaagggc gagaaggcag cggacgccct gggcgacggc atgaaggacg ccgggaagga 7261 cgttgagcgg ttcgaggaca aggcgaaggg cgcgttcaag tccctgggcg attccgcccg 7321 gaagtccggg gacgtggtgg ggtccgccat gaaacagggc acgaaggagg ccgaaggcgg 7381 cctggaggac ttcagggacg aagccaacag cacggcgaag gaaaccgccg cttcctttga 7441 cgggtccttt gattcaatcg cgggcatggc ccaggaagtc gcggcaaacg cctttgccgg 7501 gttcggcccg gcgggcgctg tcgcgggcct ggccgccgcc gccggtatcg gtatcgccat 7561 aacccaaatg caagcgggcg ccgaagcggc caacaaaatg aacgaagccg ccgtggacct 7621 ggcgggccaa atcgtggagg ccggtggcga catccgggac gtggacctgg gcgccattgt 7681 gtccgcctgg ggccgggaag tcatcgaaga caactggata acgccctggg ccaacgaagc 7741 gtccacaaag ttccaggaga cagccaagga cgcccaggac gctaaccggg acgttgcgga 7801 atccgtgcgg gccgccgccg ggtccgccga cgactccagg aaattcctgg acgacaccgc 7861 cgaaacctgg cagcgactga cacgccgcat ccaggaaggc accaccacca ccatggacgg 7921 gctgcccgtc caggacgaag cggcccgggc cgcccagaag gaacgcgacg ccctttcgga 7981 cctgcgcggc caggccgagg agaacatcaa aaccaaggaa gcggcggtgg acattgccga 8041 gattgaaacc gccgccaccg gggacaacac ggcagccatt gaagcgaaca ttcaggcgct 8101 ggaggacaag gccgccgcca tggccgacgc cgccggggta gccatggacg ccaccaccgc 8161 cgaaatccag tacgccgaca ccatggccca gggcgcgaag gacatagcgg cgaacggaaa 8221 gggcctggaa ctgaacacgg ccaccgggcg ggcgaaccag caaaccctgg tggatatggc 8281 caaatccgcc aacgagctgc gcgacgccca ggtggcggcg ggcgggtcca cggcggacat 8341 aacggccaaa caaaaccagg cccggacagc gttcatcaac gccgctgtag ctgccgggat 8401 gggcaagacg gaggccggga aactggccac acagtacggc ctgattcccg ggaacgtgga 8461 cacctacgtg aaggcccaca acgtccagaa aaccaaggat gaaattgacg gcgtggccga 8521 accccggacc gtccccatcc acctgacgcg gggcagcgag tccattagtt cctggattca 8581 gggcctatcg ggccggacta tcccggtgaa tatggccgtc cgtggtgggc gggccgtgga 8641 cgactagagg aacccgacaa accgcggccg ccggccagga tccaaaaaaa ccctagaaca 8701 cgcggaaccc tgaaaagggc tcatgtagga acccaacaaa aggcaggaca ccatggcagc 8761 gattagcgcc gaactactcc cccagtccgc cgccgtgcgg ctggcaatca acgcgcccgc 8821 cggtatccgt tcaatcaccc ggagcgacgc taacggtgtc aacgaggtcc gccccggccc 8881 cggcgtcctg gacgtacagc cgcccctggt ggacgacgcc acaaacctgg tgccgaaccc 8941 gtccttccag gtcaacacca ccggctggac ggcgtcgcgt tccgtggtca cgtcctacac 9001 gttcgccggt agcttcctga actaccccgg ccagctggcc gggacaaagg gcgtgaaggt 9061 agtcccggac ggcaccgtgg gcaccgtgac cacgtccgcc ggggacttca ccgtggcccc 9121 cggggcatgg gtggcggtgg ccgggaacgt gtcagcttcc gccgggaacg cccgcctggg 9181 catcgcgttc tacaacggtt ccacctggct gtccgaaacc tattcggacc cgattccggg 9241 cggcacgtcg gtctacaaac tcacccgcct gtccgtggcc gcccaggcgc ccgccaacac 9301 gaccaaatac cgtctgctgg tccggctgta cgctgcggac ggttccgccg cccctggcgc 9361 gtcctgcacc gtctacgggg acaagttcag cgccaccccg gacgcgggca gcgaagccgc 9421 cgccctggcc caggtggcgt tctaccacga cggcagcaca tccgattccc ccatggaatt 9481 tgaaacgtcc tggtccggca cggcccacaa cagcgcgtcc accagggcgc ggcctgtcac 9541 gccgttctat gtcacggact acgaagccgc atcggggcct atccgctatg acgtcgtgga 9601 cgggaacggc ctgttcgaga ctctggacgt gaacggtttt gtcctggact ctccctggct 9661 gttcacgccc atcattccgg gctattcccg ccagctgaga accgtccggg gcatcggggc 9721 ggacctggag aacctgtcta ccgtccatgc cgggctgctg ggccggacgg accccattgt 9781 ggtgatgcgc ccggtggggc tgcggtccgg gaccatgacc atctgggccg ggacctacgc 9841 ggcggccctg gacattgttt cgccgttgaa acaggccacg cccatgatgc ttaggcagcc 9901 ggaccacgcg ggcctagata tgtactttgc cccgctgggc gcggccctgg tccacgagga 9961 ccaggaaggc gccgggacca cctggggagt ccaggtccga taccaggaaa tcaaacgccc 10021 ggaaggtgac atagcgggcg ccctgggctg gacctacgcg gacctggaag ccgccgtgcc 10081 ccgctattcg gacctgcccc ggacctttgc cacctacgcc gatatgcgac tgaacaagag 10141 gatcacgcca tgaccgccac caccgcccgg gccggacgct accaggaccg cgccctggac 10201 atcgtgaaac agtcccaccg ccaggacgcc gccgggcagc tggaagtcct gggcgccgcc 10261 ccggaaaccc tggccgtggt gctgcgggac cctgttgtga cctttgccga ggactgggcg 10321 ccctacgtcc aggtatcggc ggacggcgtg gccccggtgg acgtggcgga actggccaaa 10381 atcgaccccc gccaggccgt ccaggtggaa gtgaaggcgg gctatgtcta cgacgacggg 10441 gaagccgacg ttcaacgcct ggccctggtc cacctgcgaa gccgccgggc ggtcctgccg 10501 gacgggaaca tgcccctggt gggcgagtcc gccgaacgcc tggcccagga cgccaaatgg 10561 ctgcccgcca cgattaccaa gacattcccc ggcgtcctgg aggccatgca atttatggtc 10621 aactacgccc agggccacac ggacgccgtc tggataacca ccatcgaacc cggctggcgc 10681 ccggacctgg tcacgtccgt ggtgctggaa caggggacgg aactgtggga acccctggcg 10741 gccctggcgc tgtccgccgg gctgcggctg tgggcggacg aggacggcgc ctggaacctg 10801 gccccgaaaa ccaccctggc gggcgtcacg gcggccttcc tgaaacaagg cccgaatacc 10861 accgtcaaaa aggtggagga cgtactaagc cgggaaggct ggtacaacgc ggcggccctg 10921 acgtaccagt ggaaggacgc ccagggcgtt gaaaagaccg tggtgggcac ctggtcccca 10981 gcggcgcccg ccgggctgga caagggcgcg ggctgccgga cctacgcggc ggaccggccc 11041 ggccccgtca gtcagtggca agcaaacgag aatgcccgcc tgacggtggc caacctgtcg 11101 tcccggggct ggtcctacgt ggtggattcc gtggccatgt actggctgcg gcccggtatg 11161 accgtccagg tggacctggc gaacggcacc acggcccggc acattgtccg ccgtgtcgtg 11221 ttccaggtgg ccgaaggaac catgaccgtg acaacccgag aacccagcaa cacagaggag 11281 agctaacacc atgcccgtaa caactaaccg cgcctaccga taccccgggg cgggcgttga 11341 acccgacgta aacggcgaca ttcagcgcct gggcgaggac gtggacgccg acatggccgc 11401 caccctggcc gccctggcga agctgcctgg ccgcatccag acggggtccg ccaccatcag 11461 catcgggtcc gccgtcgcgt ccgccaccca ggtgattacc ttcccggtgg cgttcacttc 11521 cacgcccaac gtgttcctac agtccacggc gaacgtcgcg ggccgggcgt cgctgctgtc 11581 gctgtacgtg aacgccacca gtgctaccca gttcacaacg aagattcaga cggcggacaa 11641 cgccaacacc ggcacgtcct acgcgattgc ctacaactgg attgcggttc tgtagtggag 11701 ttctatctgc cgcacaacgg ccccaggacc caggtattcg ggaacatcca gccggacggg 11761 cacccccacg ccggggacga ttttgggtac accggggcgg acggccaggt gcaccccgag 11821 gtctacgccg ctgcgcccgg cgtggtgctg tacgccgggg actccagggc gctgggctgg 11881 ccgaacccgt acttcctgaa cccggacttt gaccgcacgg acgcccggga cgattccgcc 11941 gggaacgccg taatcattcg gcacgacgac ggcggcatga ccacctacaa ccacctggag 12001 tcctggagcg tccgccgggg ccagcgcgtg acgggccgcc agcgtatcgc caccaccggg 12061 tccaccgggc ggtccaccgg gaagcatttg cattttgagt ggattcccta cccctgcaat 12121 ttcaacaccc cgacgtatgg ccgggtccgg cccacgttca aaccgagagg aatatttatg 12181 tacctgaccc agaaacagga actggaagtg ctgacagctg cccgggctat caatctgcgg 12241 gctaagtacc tggacgcccc ggtgtccgcc gtccccagga agtccgccgc cgagtacctg 12301 gacaccgcca ttccccggaa gggcgggcgc accgggaaca ccacgccccg caacaccatt 12361 gcgtaccagg acgccaacct ggacgccgtg aaggcagctg cggcggcccg ggaccaggcg 12421 gtggccgaat gaccgccccg gcgccccgga acgtcggccc ggtcacgacg ggcgctagcg 12481 cgggctatgc cgggtccgtg gtcctggtct atgtcatcga acagttgaca gcgcgggacg 12541 tgccggaccc ggtggaactt gccctgggcg tcctcctgac ggtgggcggc gggttcctgg 12601 tccgctccag ggcggtggcc ggtggctgag gcgccggtcc aggacgtgac ggcctggcgc 12661 ctggggaaga ttgagatcgc cctagacgcg gtcctggtca agctggacga ccgcccggac 12721 tggaaggacg ttcgcaacat cgaggaccag ctggcggcca gaatcaccag gctggaggac 12781 tggcaaacct gggcaatccg catcgtggta ggcgcggtcc tgctggccgt cgtcggcctg 12841 gccctggctg tcgggagaac ctgacgtttt gtaggttccg tcaagccggc ctttttgaaa 12901 aaccccggaa tctagggact ttcacaaagg ccggcctttg agctccgctg taggaaccct 12961 acaaagggcc gcccaggcgg acgggaccac gccacgcggg acacgctggc gtggtcctgt 13021 ctgcaaggtt ccttgcctta tgctttcaac ggttgcaagg gaccttgcaa ctacggaggc 13081 aaacgaagtg aggctatgca aatgcccgga cgctacagac accccgacga cacggcccgc 13141 gtggtcacgc ccagcgacct aatcggcgcc caggaggctt gtgcactcct gggaatcgac 13201 cggtccaccc ttacccgctg gattcagtca ggggccaccc tgccgctggc ccagctggac 13261 ggcccgggcg gcgcctacgt gttcgacagg acggacgtgg aggcccaggc acgaaacatt 13321 caggccgcca cggcagccat gcccctggtc aagaaaggac cacaaaagga atgagagcac 13381 gacctgtatt cgtcagcatc accgacgccg ccaacatcct gggcgtatcc gccaggaccg 13441 tgaagcgcta cgtggcagac ggacacctgg acgcgggcga gctgccccat gggcaggtac 13501 gcctggcccg cgcccaggtc attggcctgg tacgaactac cccgaggaac ccggaatgaa 13561 ccaacctgga aagactttca cagttaggcg tggacagaca gccatgccta accgcacgat 13621 ctacgacagc tacacgtccc tgggcggcgg cggcgaggac gcccgccagc tgtccattca 13681 ggcgctgggc gtcctgctgc tgggcctgtc gcggcctgac accgccgccc agggctaccg 13741 cgccttcctg ggccggggcc tgggccggga cgggctgctg aaggccctga aggaactgaa 13801 cctgacgggg caccgccacc agttcaaacg caacggaccc ggcggccaca tggtcacgga 13861 cacgctgctg tctgaggtcc ccatgacccc ggacgcggcc agcgaggaat ggaaggcgct 13921 ggtgctggcc atggacgccg acgcccgcca ggcagccaag gacggggcgg cagcctacgc 13981 ccgccaggcc gaagccatgg cccgggaact ggcggaacag gacgccgccg aagctgcggc 14041 agcctgtcag cggctggcgg accagctggc cgccgaagcc gccggaatgc ctggccgtga 14101 ccgtgcgccg gaaaacggag catcgggcac tgtggagggg tccgcaccgt gcgacggttc 14161 accggcatac ggtgaagcgg cgcatctatc tgtaccagat agccaagtct ctaaagagac 14221 ttcgtccttt agagacagag acaaccaagc aagcgagaaa agcgaggacc aggaacggcc 14281 agcacaaggc ggggccgccg tgtcgaatga cgcggccacc ggacccggcc acgcggcagt 14341 cctggacatc ctggccgaac ggcggtccaa gatgcgggca ctcaaaacga agtcgctccg 14401 ctccggccat ggacaagagg cgggcgcgtg atgagtcggg cgacggacaa gacgctgcgg 14461 gcggcagcat tcaaagaggc cgcgttcacc gcacgtatgg ctgaggagtc caggaagcgg 14521 gccagggccg cctggaagga cgcccaggcc cgtggggctg ggcaggacga attgagggcg 14581 ttagaagtcc aaacacggat tgcctacgcc agggccacca cggcggcgga cctggaggac 14641 gaagcctggc agcactacgc cgccaccctg ccccatggct aggcgtccgg caccgcccca 14701 gggccagctg ttccgcatgg acccggtgca agtcccgggc caggacgggc accgggccaa 14761 gaagtggggc ggacgcaagg tggcgaaggc ccgggacacc gtaagggcca tgctgcccgc 14821 cgcttgctgg aggggctgcg gacggatgat aaccagggac tggccggaaa aggactggca 14881 cgccgggcac gtcaacggac gggccgaagg cgggccggac aacgtaacga actacctacc 14941 cgaatgccgc tggtgcaacc tgtcagaggg cggcaaggtg ggcgcggcaa tcacgaacgg 15001 aaagcgcgtg gcaatcacca cggacgcgat tagggaaagg accctgaagt ggtactagag 15061 aacgaaaccg gcgaaccagg gacagacctg ttccgctgcg acagctgccc ccaggaaatc 15121 gagggccgga cctggacctg gctggaacgc caggtgtcca accacacccg ggccaacaag 15181 ggccaccgga tgttcatggt cctggacggt gaagcatgag ccgcaagggc ggcgggcagc 15241 ggcgccacgc catggacccc gtcacgctgg tgcacatggc caggctggaa cccgctgcgg 15301 cccagtacat cctggaccgc cgccgccgtg gggtcccggc ccggggactg gggcggccat 15361 gaacccggga ctggaccagg acctggccgt gctggcgctg ctgctgctgg cgctggtcct 15421 ggtggctgcg ggcgtcgggc tgtggtggga cagctgccgc cgccggggcc acgaggacct 15481 ggagctaacc gccctgtgca acgacgggcg cgtggcatgg cgggcgcggc ggtgtcagcg 15541 ctgcggcagc ctgagattcg aggaactggt atgagttttt cgacagaggc cggaagccca 15601 cgtataccgt cc //